~【NEW!!活動快報】~ 全景玻片影像掃描器,精準對焦與掃描,染色結果從此數位化
MoticEasyScan 全景玻片影像掃描器,3D多層次掃描,快速對焦精準掃描為數位玻片。提供最佳解析度0.25um/pixel ;6片式片夾,walk-away設計。搭配簡易操作軟體,優質影像數位永久保存。歡迎來電洽詢(02)86609496許虹宜

目前日期文章:201106 (25)

瀏覽方式: 標題列表 簡短摘要

太鼎生物科技 發表在 痞客邦 留言(0) 人氣()


PEG病毒濃縮產品,有效提高病毒力價。可應用於Lentivirus 病毒濃縮,提供病毒力價或環境sample病毒的濃縮.


PEG Virus Precipitation Kit (Biovision)
Catalog#: K904-200  | Size: 200 preparat



業務專員 許 虹宜 0920312382



 


PEG病毒濃縮產品特點:


 


不須超高速離心


安全無毒性


可將病毒液濃縮10 100


適用於 LentivirusRetrovirus、等病毒。


包裝為5 倍無菌濃縮液方便使用


 



 


PEG病毒濃縮產品介紹:




• Kit size- Convenient sizes (50 and 200 preparations)
• Application- The kit can be used for small lab samples or large scale virus preparation with high yield and high viral titer and the kit can be used to concentrate retroviruses, baculoviruses, lentiviruses, and phages etc in cell culture medium or environmental samples.


Features & Benefits:
• A quick, easy and inexpensive method is desired to concentrate virus and remove impurities
• Easy, convenient and time-saving method to concentrate virus without ultra-centrifugation
• Virus can be concentrated over 100 folds
• The whole process uses non-toxic reagents


 



 


PEG病毒濃縮產品規格:


 



Kit components:
• PEG Solution (5X)
• Virus Re-suspension Solution (1X)


 


Description:
Virus was usually produced at a low titer. It often needs to be concentrated for storage or further applications. A quick, easy and inexpensive method is desired to concentrate virus and remove impurities. BioVision’s PEG Virus Precipitation Kit provides an easy, convenient and time-saving method to concentrate virus without ultra-centrifugation. The kit can be used for small lab samples or large scale virus preparation with high yield and high viral titer. The kit can be used to concentrate retroviruses, baculoviruses, lentiviruses, and phages etc. in cell culture medium or environmental samples. Virus can be concentrated over 100 folds. An optimized Virus Re-suspension Solution is provided to maximize viral recovery by 40-100% depending on the virus type and sources. The whole process uses non-toxic reagents. The concentrated virus can be used for infection, viral DNA or RNA purification, etc.


 



 


PEG病毒濃縮產品貯存:


Storage Conditions:



-20°C

 


【太鼎生物科技有限公司02-86609496www.biopioneer.com.tw


 

太鼎生物科技 發表在 痞客邦 留言(0) 人氣()




 

西方轉漬分析-ECL【市售ECL主要市場比較】

太鼎生物科技有限公司 (02)86609496



業務專員 許 虹宜 0920312382



www.biopioneer.com.tw




 


 


1. PerkinElmer


市面上大部份ECL的等級是nanogram,PerkinElmer Lightning plus靈敏度為1-10 picogram,包裝規格如下: 170mlx4 特價~7000元  (~10元/ml)


Western Blot Chemiluminescence Reagent Plus



(~10/ml)



 




















目錄號碼

包裝

可使用面積

NEL104

2 x 170 ml

2,500 cm2 membrane

NEL105

4 x170 ml

5,000 cm2 membrane



2. Pierce


SuperSignal West Pico Chemiluminescent Substrate 


靈敏度為picogram 250mlx2   特價~7000元  (~14元/ml) 第二件半價 (~10元/ml)SuperSignal West Pico Chemiluminescent Substrate 


(~10~14/ml)



 


T-PRO新一代高靈敏度冷光試劑ECL


 



() fetagram ECL,靈敏度接近fg (靈敏度接近millipore ECL)


200mlx2 特價3500元


() Picogram ECL,靈敏度接近pg (靈敏度高於Pierce ECL, PerkinElmer )



200mlx2 特價2500元



 



(~6.25/ml)-->picogram等級


 




 

 



【太鼎生物科技有限公司02-86609496


 



(~8.75/ml)-->fetogram等級



 


T-Pro LumiFast Chemiluminescence Detection Kit, 貨號#JT96-K001S


400ml特價2500


T-Pro LumiFast Plus Chemiluminescence Detection Kit, 貨號#JT96-K002S  400ml特價3500



 



冷光試劑介紹:


T-Pro LumiFast Chemiluminescence Detection Kit & T-Pro LumiFast Plus Chemiluminescence Detection Kit  為一方便用於偵測直接或間接帶有HRPWestern blottingSouthern Northern化學發光之試劑套組。在有過氧化氫存在的情形下,Horseradish peroxidase (HRP)會催化環狀二酰基酰肼(cyclic diacylhydrazides)的氧化,像是發光胺(luminol)。氧化當下,發光胺(luminol)的中間產物處於激發態,此產物藉由發光的方式衰退為基態。強化劑可增強發光的能量。利用此方法可以藉由直接或間接標定有HRP的抗體/鏈親和素(streptavidin)進而偵測特殊抗原或是核酸序列。


操作流程:


1.      T-Pro LumiFast Chemiluminescence Detection Kit & T-Pro LumiFast Plus Chemiluminescence Detection Kit 中的試劑A及試劑B等體積混合至足夠蓋過membrane的使用量(平均用量最少為0.1ml/cm2)。於室溫使此混合試劑均勻混合作用1-2分鐘。


2.      membrane上過量的緩衝液倒掉,但要保持轉漬膜的濕潤。加入步驟1混合好的試劑於轉漬膜上,在室溫下作用1-2分鐘。


3.      將步驟2過多的混合試劑倒掉,且將轉漬膜置於兩層保鮮膜中間或用兩片透明塑膠片密封,注意不可以有氣泡,並將轉漬膜吸附蛋白正面朝上,同時轉漬膜請勿過濕。


4.      將步驟3轉漬膜置入底片夾中。置入底片,要避光或是使用紅光。曝光約3090秒。


5.      置入新的底片前,請先將原先片夾中的底片取出,並將底片放入顯影劑中。


6.      第二張底片的曝光時間可依據第一張的時間做調整。


7.      如果訊號過強,可在加入呈色劑15~30分鐘後再進行壓片,或作其他時間的調整。


注意事項:


1.      轉漬膜在接上一級抗體後須保持濕潤,不能乾掉。


2.      請在壓片前再配製化學發光試劑,配製量足夠覆蓋轉漬膜即可,勿將使用過混合試劑重複或混合使用。


3.      請使用乾淨微量吸管(Tip)並單一取用每種試劑,勿重複多次試用避免污染。


4.      除了取用底片、壓片曝光及洗片處理外,其他步驟可不必在暗室中操作。


5.      壓片後轉漬膜可經由T-Pro Western Blot Stripping Reagent  (JB11-K002) 洗去原有抗體後,重複再次使用。

太鼎生物科技 發表在 痞客邦 留言(0) 人氣()


進行細胞 immortalization,只須將定量好的SV40 Lentivirus 感染細胞,即可讓細胞immortalization,其不須要任何的抗生素篩選,即可讓primary的細胞成為immortalize細胞株。客戶不須再自己養病毒, 表現病毒及定量病毒力價!!



SV40全名猿猴空泡病毒40Simian vacuolating virus 40)或猿猴病毒40Simian virus 40),是一種多瘤病毒,也是一種DNA病毒,有造成腫瘤發生的潛在能力,不過通常會維持於潛伏感染(latent infection)狀態。這種病毒是在1960年,於用來生產脊髓灰質炎疫苗恆河猴腎臟細胞中發現。



abmgood一系列病毒表現產品. 幫您生產好重組sv40 Lentivirus病毒,且經由QPCR定量Lentiviru的病毒力價( 106 c fu/ml),立即可以感染細胞,即可讓細胞immortalize, 培養液不需添加抗生素, 針對對抗生素敏感的primary cell, 可以cultrure更多代。另有更多相關產品包括hTERT,p53,Myc,Rb,Ras V12, SV40 T antigen等都是做好的lentivirus病毒,可以直接進行細胞感染。



 


產品貨號 Abmgood #G258                太鼎生物科技有限公司代理02-06689496



 








































Product



Vector Map


(Click to View)



Description



Quantity



Cat. #



Adeno-SV40 Adenovirus



pAdeno-SV40



Adenovirus expressing SV40 large and small T antigens (106cfu/ml).



250ul



G210



Retro-SV40 Retrovirus



pRetro-E2-SV40



Retrovirus expressing SV40 large and small T antigens (106cfu/ml).



10ml



G212



Lenti-SV40 Lentivirus



pLenti-SV40



Lentivirus expressing SV40 whole gene (106cfu/ml).



10ml



G203



Lenti-SV40T Lentivirus



pLenti-SV40-T



Lentivirus expressing SV40 large T antigen (106cfu/ml).



10ml



G256



Lenti-SV40 T+t Lentivirus



pLenti-SV40-Tt



Lentivirus expressing SV40 large and small T antigens (106cfu/ml).



10ml



G258



 


 


 


 



業務專員 許 虹宜 0920312382



 


詳細產品資訊,請登錄www.abmgood.com 查詢,或與太鼎生物科技有限公司(02)86609496聯繫。 


WWW.BIOPIONEER.COM.TW


太鼎生物科技有限公司(02)86609496


 


 

太鼎生物科技 發表在 痞客邦 留言(0) 人氣()

 


 太鼎生物科技有限公司(02)86609496


                                                        

業務專員 許 虹宜 0920312382





Lentiviral 表現系統介紹

   As a world leader in lentiviral technology, ABM has developed a variety of different lentiviral expression systems which can be used to temporarily alter expression within a sample of cells or to moniter gene expression using reporter genes. These systems allow other scientists to produce their own lentiviruses.


 


Lentiviral 表現系統產品種類


Lentiviral Systems


Lentivirus Constitutive Vectors表現載體


Lentivirus CMV啟動子載體 


His Tag Lentiviral 載體 | HA Tag Lentiviral 載體 | HA-III Tag Lentiviral 載體


Lentivirus UbC, PGK or EF1α啟動子載體 


UbC Promoter Lentiviral載體 | PGK Promoter Lentiviral 載體 | EF1α Promoter Lentiviral 載體


Lentivirus 啟動子缺失載體


 Promoterless Lentiviral 載體 | Promoterless GFP Lentiviral 載體


Bicistronic/Tricistronic Lentiviral 載體


shRNA and miRNA Lentiviral 表現載體


RFP Fusion Tag Lentiviral 載體


綠螢光蛋白GFP Fusion Tag Lentiviral 載體


綠螢光蛋白GFP Bicistronic Lentiviral載體


Reporter Lentiviral 載體


Therapeutic Lentiviral 載體


 


Lentivirus 包裝系統


   As a world leader in lentiviral technology, ABM has developed two unique packaging mixtures. Each contains two or three plasmids to seperate the lentiviral structural and replication genes until time of viral production, and preventing the structural genes from being present in the packaged viral genome of the produced virus. This increases the safety for the user by making the produced viruses replication incompetent.


Products List


Lentiviral 包裝系統


產品介紹


  For the production of lentiviral particles, three components are generally required: 1) a lentiviral vector containing your inserts of interests (cDNA, shRNA, or miRNA), 2) one or two packaging vectors which contain all necessary viral structure proteins, 3) an envelope vector expressing Vesicular Stomatitis Virus (VSV) glycoprotein (G). The 3rd(third) generation packaging system offers maximal biosafety as the lentiviral Rev gene is supplied as an independent vector from other structure genes, further eliminating the possibility of reverse recombinantion of vectors into a replication competent viral particles. The 3rd(third) generation lentiviral packaging mix will only support lentiviral expression vector with a chimeric 5' LTR in which the HIV promoter is replaced with CMV or RSV, thus making it TAT-independent. Thus the 3rd(third) generation lentiviral vectors will not support the production of 2nd(second) generation lentiviral particle productions. All of the lentiviral vectors marketed by ABM are TAT-independent.


2nd Generation Packaging Mix





 



























Product



Vector Map


(Click to View)



Select. Marker



Quantity



Cat. #



2nd Generation Packaging System Mix



pLenti-P2A
pLenti-P2B



N/A



200ul



LV003



GFP Control Lentivirus



pLenti-GFP



Neomycin



10ml



LV006



β-Gal Control Lentivirus



Map Unavailable



Neomycin



10ml



LV007



3rd Generation Packaging Mix





 

 



























Product



Vector Map


(Click to View)



Select. Marker



Quantity



Cat. #



3rd Generation Packaging System Mix



pLenti-P3A
pLenti-P3B
pLenti-P3C



N/A



200ul



LV053



GFP Control Lentivirus



pLenti-GFP



Neomycin



10ml



LV006



β-Gal Control Lentivirus



Map Unavailable



Neomycin



10ml



LV007



 


Lentivirus純化及定量套組


 


Lentivirus 純化 Kit





















 


Product



Description



Cat. No.



PuRetro™ Lentivirus Purification Kit



2 Stardard Units



G171



PuRetro™ Lentivirus Purification Kit



5 Stardard Units



G172



PuRetro™ Lentivirus Purification Kit



2 Giga Units



G173



 


PuRetro™ Lentivirus Purification Kits產品特點


easy to use


very reliable


very affordable


Speedy Lentivirus 純化


 


 










Cat.No.:



LV999



Quantity:



100ml



 


 


Speedy Lentivirus 純化產品特點


  Recombinant lentiviral vectors have been shown to be a powerful tool for stable gene transfer to both dividing and non-dividing cells in vitro and in vivo.Through years of experience with lentiviral vectors, ABM has developed its own proprietary pLenti-combo packaging mix and an efficient protocol for rapid production of recombinant lentiviral vectors with titers up to 107IU/ml. The quickest and easiest way to purify and concentrate lentiviral particles up to 100x. The concentrated viral preparations can be used for in vivo injection or like applications that require high titer of viral particles.


 


ABM's Lentiviral表現載體產品特點


 


stable plasmid; rare plasmid DNA re-arrangement


very high yielding plasmid


no special competent cells required


His and HA tag compatible


ABM is THE source for your lentiviral expression needs!


 


Viral Stock Buffer Exchange Kit


 










Cat.No.:



G130



Quantity:



5 units



 


Viral Stock Buffer Exchange Kit (Cat.# G130) is supplied separately from the recombinant viral purification kits. All recombinant viral purification kits do not contain the viral stock buffer exchange kit.
  The eluted recombinant virus is in a salt buffer but can be used directly for in vitro target cell transduction if the viral dilution is over 5X (i.e. 1.0μl of viral stock to 4μl culture medium). For higher titers of viral stock or viral dilutions less than 5X during transduction, a buffer exchange is required to make the viral stock suitable for such applications. This can be easily performed using ABM Inc.'s viral buffer exchange kit (Cat.# G130). The kit includes five exchange filters that are applicable for all recombinant viruses, as well as two specialized buffers, in sufficient quantities to run either five adenoviral or five lenti/retroviral or any combination of purifications.
Adenoviral storage buffer: 2.5% glycerol (w/v), 25mM NaCl, and 10mM Tris-HCl, pH 8.0 (Hoganson, et al., 2002).
Lentiviral/Retroviral storage buffer: 1X PBS buffer, pH at 7.2.


 


qPCR Lentivirus Titer (Titration) Kit



 










Cat.No.:



LV900



Quantity:



100 Reactions



 


The Lentivirus Titration Kit provides a fast and simple method for titrating lentivirus. This kit employs a quick RNA extraction step and determines viral RNA using qRT-PCR. The whole assay could be completed in only 2 hours. The titer primers supplied in this kit are designed for all HIV-1 based vectors detection. With the help of the on-line titer calculator, titration of any lentiviral preparation could be easier than ever before.


 


ABM's Lentiviral qPCR Titer Kit病毒力價定量產品特點


 


no RNA purification -- saves time and eliminates inaccuracy


ready-to-use reagent mix -- reduces variability


qRT-PCR in one step -- more sensitive and accurate than other methods


no NTC amplification -- our titeration kit is the only one on the market that completely elimites NTC signal due to our unique primer design. Similar kits from other companies all give NTC amplification, which compromise accuracy for viral titeration


free qPCR mastermix -- a deal that can not be beat


 


詳細產品資訊,請登錄www.abmgood.com 查詢,或與太鼎生物科技有限公司(02)86609496聯繫。 


WWW.BIOPIONEER.COM.TW


太鼎生物科技有限公司(02)86609496

太鼎生物科技 發表在 痞客邦 留言(0) 人氣()

彗星試驗結果分析方法



業務專員 許 虹宜 0920312382




 





 



1.      將影像儲存為 8-bit BMP圖檔。


2.      開啟彗星試驗分析軟體                         


3.      開啟新檔案 File | Open  選取欲分析的圖檔。


4.      調整右邊工具列Cut off值,避免過飽和。


5.      選定欲分析之細胞,按住滑鼠左鍵,拉出一個長方形框,將分隔線對準細胞核的中心。


6.      出現儲存檔案的視窗,將檔案命名後(*. txt)儲存。


7.      再隨機選取75~100個細胞進行框選分析。


8.      可以將框選結果另存新檔 File | Export BMP,將檔案命名後儲存(*. BMP)


9.      結果顯示:打開儲存的txt檔案,選擇另存新檔,將檔名命名為*.xls


10. 結果分析:可以選擇Tail Moment作為計算指標。


 


太鼎生物科技有限公司  02-86609496 


每台電腦要用此軟體分析前,先讀取有距離刻度的標準圖片,


選取Setting | calibration ,按+或減,長度會改變,若長度為五個刻度,請按右鍵在數值處輸入5即完成校正工作






 


 


 


 


 


 


 


 


 


 


 


 


 


 


使用TriTek CometScore™ 免費軟體分析彗星試驗結果之參考文獻


1.      M. Aghi, S. Rabkin, and R.L. Martuza. 2006. Effect of Chemotherapy-Induced DNA Repair on Oncolytic Herpes Simplex Viral Replication. Journal of the National Cancer Institute, Vol. 98, No. 1, 38-50


2.      E. Gallmeier, J.M. Winter, S.C. Cunningham, S.R.Kahn and S.E. Kern. 2005. Novel genotoxicity assays identify norethindrone to activate p53 and phosphorylate H2AX Carcinogenesis vol.26 no.10 pp.1811–1820


3.      R.V. Pusapati, R.J. Rounbehler, S. Hong, J.T. Powers, M. Yan, K. Kiguchi, M.J. McArthur, P.K. Wong, and D.G. Johnson. 2006. ATM promotes apoptosis and suppresses tumorigenesis in response to Myc. PNAS 103;1446-1451


4.      J. R. Alt, A. Bouska, M.R. Fernandez, R.L. Cerny, H. Xiao, and C.M. Eischen. 2006. Mdm2 Binds to Nbs1 at Sites of DNA Damage and Regulates Double Strand Break Repair.Joural of Biological Chemistry Vol. 280, No.19, pp.18771–18781


 

太鼎生物科技 發表在 痞客邦 留言(0) 人氣()

                         RNAi專用的轉染試劑


                        RNAifectin™ Transfection Reagent, G073



 


【太鼎生物科技有限公司02-86609496abmgood台灣總代理


 










Cat.No.:G073
Quantity:1.0ml (100-500 transfections)





















Description



Escpecially developed for the efficient transfection of eukaryotic cells with RNAi oligo's. This transfection reagent is perfect for any of your RNAi transfection needs.



Application



RNAi knockdown experiments in a wide variety of cell lines (HeLa, HEK-293 etc). Highly specific and non-toxic transfection.



Protocol





Storage



Store at 4ºC. Do not freeze.



Notes



This product is distributed for laboratory research only. Caution: Not for diagnostic use .



產品介紹 Description


ANAifectin™ is a transfection reagent specially formulated with multiple cationic polymers. It is suitable for the transfection of RNAi oligoes into cultured eukaryotic cells.


RNAi轉染操件手冊:


These conditions are recommended as guidelinesonly. A 6-well or 35mm dish is adequate for most applications, but larger vessels are sometimes required. In that case, consult table 1.



1. In a 6-well plate, seed cells at a density of 1-3x10⁵ per well in 2.0ml of the appropriate
growth medium (with serum if cells are cultured in presence of serum). Incubate the cells at 37°C until cells are 60-90% confluent. This will usually take 18-24 hours, depending on cell types. Since transfection efficiency is sensitive to culture confluence, it is important to maintain a standard seeding protocol from experiment to experiment


2. Prepare the following solutions in sterile 12x75 mm or microcentrifuge tubes: Solution A: For each transfection, dilute 1-3μg of RNAi oligoes into 125μl serum-free
medium. Solution B: For each transfection, dilute 4-10μl of
RNAifectin™ reagent into 125μl of serum-free medium. (Vortex RNAifectin™ reagent before use)


3. Combine the solutions, mix thoroughly and incubate at room temperature for 20 mins to
allow DNA-lipsome complexes to form.


4. Remove growth medium from the cells and add 800μl of serum-free medium to the cells.


5. Add the RNAifectin™-oligo complexes to thecells, mix gently to ensure uniform distribution
and incubate at 37°C for 2-24 hours. We recommend starting with 5 hours.


6. Remove the transfection solution and add 2.0ml of the appropriate complete growth medium(with serum) to each well. Incubate cells at 37°Cfor a total of 24-72 hours.


7. Assay cell extracts for gene activity 24-72 hours a􀄞er the start of transfection depending on the cell types and promoter activity.


8. A similar procedure can be used to transfect an RNAi vector for stable expression. At 72 hours a􀄞er transfection, split the cells at a ratio of 1:10 into the selective medium for the marker gene transfected.


【太鼎生物科技有限公司02-86609496abmgood台灣總代理



業務專員: 許 虹宜Hung-Yi Hsu       


電話: 0920312382




太鼎生物科技 發表在 痞客邦 留言(0) 人氣()

文章引用來源:http://proj.ncku.edu.tw/research/articles/c/20100326/2.html




細胞核內EGFRSTAT5蛋白相互作用能活化Aurora-A基因的表現





Aurora-A激脢在許多的腫瘤細胞以及癌細胞株中均有大量表現的情形。研究顯示,Aurora-A激脢是一個致癌基因1。目前認為,在腫瘤細胞中Aurora-A激脢表現量的增加,除了來自DNA的增幅、蛋白質穩定度的增加之外,還有其基因轉錄的活化2。對於在腫瘤細胞中Aurora-A激脢基因轉錄的調控機制,目前則還不清楚。另外,表皮生長因子受體(EGFR)是一種位於細胞膜上的受體酪胺酸激脢(receptor tyrosine kinase),在許多的腫瘤細胞以及癌細胞株中亦有高量的表現。近幾年來,有許多的研究證據顯示,表皮生長因子受體在受到刺激之後,會由細胞膜進到細胞核內,直接扮演一個轉錄活化因子(transcription activator)的角色3。而這種能在細胞核內偵測到EGFR的現象,大多與細胞的高度增生(highly proliferation)是有關的3。根據文獻報導,會受到細胞核內EGFR所轉錄活化的基因,其啟動子區域(promoter region)都存在著所謂的ATRSs(AT-rich sequence sites)序列3。有趣的是,EGFR本身並不具有DNA結合的能力,它必須要先與其他具有DNA結合能力的轉錄因子形成一個複合體之後,才能去活化標的基因的表現。



 


圖一、(A) EGF的刺激會增加Aurora-A啟動子的活性。 (B)細胞核內EGFR與STAT5會一起結合上Aurora-A基因的啟動子。A431細胞處理(–)或不處理(+)EGF,以1% formaldehyde固定,sonication之後,收集nuclear extract,以normal rabbit IgG (NRIgG)、EGFR或STAT5抗體作免疫沈澱反應,再將共同沈澱下來的DNA以PCR的方法偵測Aurora-A promoter區域。


本研究觀察到,在高度表現EGFR的細胞株中,其細胞核內的確可以偵測到EGFR的存在;且在這些有EGFR表現的細胞株中,可以看到EGF的刺激會增加Aurora-A啟動子的活性(圖一、A)。分析Aurora-A激脢的啟動子區域,發現有五個可能的ATRSs,其中三個ATRSs則包含了可能的STATs (signal transducers and activators of transcription)蛋白結合的序列。作ChIP assay以及re-ChIP assay則發現,在EGF刺激之下,細胞核內的EGFR的確可以與STAT5蛋白共同聚集到Aurora-A激脢的啟動子區域(圖一、B)。此外,利用免疫螢光染色(immunofloursence assay)及共同免疫沈澱反應(co-immunoprecipitation assay)也可以偵測到細胞在EGF的刺激之下,細胞核內EGFR與STAT5蛋白之間存在相互作用。而在Aurora-A啟動子上,EGFR與STAT5蛋白可能結合的序列區域,也利用DNA-affinity purification assay得到確認。有趣的是,在A431細胞中,EGF的刺激使得Aurora-A蛋白激脢的增加,會造成細胞內中心體的增殖以及微小管的不規則(圖二)。利用RNAi的技術,將細胞內EGFR或是STAT5A的表現量減少,則此時Aurora-A基因的表現便不再受到EGF的刺激而增加;反之,若是在細胞株中大量表現EGFR或是STAT5A,那麼EGF增加Aurora-A基因表現的情形會更明顯,若同時表現EGFR及STAT5A則有加成的效果(圖三)。減少或是大量表現STAT5B,並不會影響EGF增加Aurora-A表現的效應(圖三)。這說明了細胞核內EGFR與STAT5A在調控Aurora-A蛋白的表現上是具有特異性的。
 


圖二、EGF處理細胞會造成(A)中心體數增加,及(B)微小管排列不規律。Aurora-A (左)或a-tubulin (右)(綠色),γ-tubulin(紅色)。藍色代表細胞核。


 



圖三、EGFR與STAT5A協同活化Aurora-A基因。


總結本研究成果,我們發現,活化的EGFR訊息傳遞途徑能增加Aurora-A激脢的表現,造成染色體的不穩定性,最後導致腫瘤的形成。

參考文獻



  1. Bischoff JR, Anderson L, Zhu Y, Mossie K, Ng L, Souza B, Schryver B, Flanagan P, Clairvoyant F, Ginther C, Chan CS, Novotny M, Slamon DJ, Plowman GD. A homologue of Drosophila aurora kinase is oncogenic and amplified in human colorectal cancers. EMBO J. 1998, 17(11):3052-65.
  2. Marumoto T, Zhang D, Saya H. Aurora-A - a guardian of poles. Nat Rev Cancer. 2005, 5(1):42-50.
  3. Lin SY, Makino K, Xia W, Matin A, Wen Y, Kwong KY, Bourguignon L, Hung MC. Nuclear localization of EGF receptor and its potential new role as a transcription factor. Nat Cell Biol. 2001, (9):802-8.

太鼎生物科技 發表在 痞客邦 留言(0) 人氣()

www.abmgood.com


 



初代細胞 (primary cells) 較一般細胞株 (cell line) 取得不易,且具有繼代培養代數有限和培養基費用較高等…問題,因此Abmgood針對一系列初代細胞、幹細胞等,直接銷售初代培養細胞,滿足即時細胞影像攝影、細胞遷移、傷口癒合、共同培養等…多樣化的實驗設計與需求


 


Products


·       Primary Adipose Cells


·       Primary Airway Cells


·       Primary Blood Cells


·       Primary Blood Vessel Cells


·       Primary Bone & Cartilage Cells


·       Primary Bone Marrow Cells


·       Primary Brain Cells


·       Primary Breast Cells


·       Primary Colon Cells


·       Primary Eye Cells


·       Primary Hair Follicle Cells


·       Primary Heart Cells


·       Primary Liver Cells


·       Primary Mesentery Tissue Cells


·       Primary Oral Cells


·       Primary Pancreas Cells


·       Primary Pharynx Cells


·       Primary Placenta Cells


·       Primary Renal System Cells


·       Primary Reproductive System Cells


·       Primary Skeletal Muscle Cells


·       Primary Skin Cells


·       Primary Umbilical Cord Cells


詳細產品資訊,請登錄www.ABMGOOD.com 查詢,或與太鼎生物科技有限公司(02)86609496聯繫。 


更多參考內容:


WWW.BIOPIONEER.COM.TW


 太鼎生物科技有限公司(02)86609496

太鼎生物科技 發表在 痞客邦 留言(0) 人氣()

Lentivirus 化產品. 其能有效增強病毒力價。



業務專員 許 虹宜 0920312382


 






















Product



Description



Cat. No.



PuRetro™ Lentivirus Purification Kit



2 Stardard Units



G171



PuRetro™ Lentivirus Purification Kit



5 Stardard Units



G172



PuRetro™ Lentivirus Purification Kit



2 Giga Units



G173



 Both recombinant retrovirus and lentivirus are widely used in the transduction of a varity of target cells and can be produced by transient transfection, from which viral supernatant can be collectedand used for target cell transduction. The titer of viral supernatant by transient transfection is approximately 106 cfu (colony forming unit)/ml, which is sufficient Purification Experimental Flow Chart for the generation of most stable cell lines in vitro. However, there are applications that require higher purity and titers. These applications include experiments demanding higher gene transduction efficiency and in vivo gene delivery. In addition, crude viral supernatants are not suitable for in vivo administration due to various contaminants contained in cell culture supernatant. Thus, the viral supernatant needs to be further concentrated and purified before use.
  Traditionally, both recombinant retrovirus and lentivirus have been concentrated via ultracentrifugation. Although the method works well, it requires specific ultracentrifugation equipment and it is technically demanding. In addition, the total viral supernatant volume is limited to the size of ultracentrifugation tubes. Reports have also stated that the ultracentrifugation process has some detrimental physical effects on the recombinant virus.
  Due to limitations on the existing technique, there is great necessity for a quick and efficient method to purify and concentrate recombinant retrovirus and lentivirus. Scientists at ABM Inc. have had years of experience with Ion-Exchange-based filter membranes and have successfully developed PuRetro™, the most effective retroviral and lentiviral purification method based on this technology.


Advantages of PuRetro™ Lentivirus Purification Kits


·        easy to use


·        very reliable


·        very affordable





















Product



Description



Cat. No.



PuRetro™ Lentivirus Purification Kit



2 Stardard Units



G171



PuRetro™ Lentivirus Purification Kit



5 Stardard Units



G172



PuRetro™ Lentivirus Purification Kit



2 Giga Units



G173



 


【太鼎生物科技有限公司02-86609496ABMGOOD台灣總代理



Citations


1.    Buchschacher, G. L., Jr., and Wong-Staal, F. (2000) Development of Lentiviral Vectors for Gene Therapy for Human Diseases. Blood 95, 2499-2504.


2.    Burns, J. C., Friedmann, T., Driever, W., Burrascano, M., and Yee, J.-K. (1993) Vesicular Stomatitis Virus G Pseudotyped Retroviral Vectors: Concentration to a Very High Titer and Efficient Gene Transfer into Mammalian and Nonmammalian Cells. Proc. Natl. Acad. Sci. USA 90, 8033-8037.


3.    Demmer, W. and Nussbaumer, D. 1999. Large-scale Membrane Adsorbers. J. Chromatogr. A., 852:73-81.


4.    Dull, T., Zufferey, R., Kelly, M., Mandel, R. J., Nguyen, M., Trono, D., and Naldini, L. (1998) A Third-Generation Lentivirus Vector with a Conditional Packaging System. J. Virol. 72, 8463-8471.


5.    Graham, R.L., Smiley, J., Russel, W.C., and Narin, R. 1977. Characteristics of a human cell line transformed by DNA from human adenovirus type 5. J. Gen. Virol., 36:59-72.


6.    Hoganson, D.K., Ma, J.C., Asato, L., Ong, M., Printz, M.A., Huyghe, B.G., Sosnowshi, B.A. and D'Andrea, M.J. 2002. Development of a stable adenoviral vector formulation. Bioprocessing J. 1(1):43-48.


7.    Hutchins, B. 2002. Development of a reference material for characterizing adenovirus vectors. Bioprocessing J., 1(1):25-28.


8.    Lochmuller, H., Jani, A., Huard, J., Prescott, S., Simoneau, M., Massie, B., Karpati, G., and Acsadi, G. (1994). Emergence of Early Region 1-Containing Replication-Competent Adenovirus in Stocks of Replication-Defective Adenovirus Recombinants (Delta E1 + Delta E3) During Multiple Passages in 293 Cells. Hum. Gene Ther. 5, 1485-1491.


9.    Wang, I. I. , and Huang, I. I. (2000). Adenovirus Technology for Gene Manipulation and Functional Studies. Drug Discovery Today 5, 10-16.


10. Wivel, N. A. (1999) Adenoviral Vectors. In The Development of Human Gene Therapy, T. Friedmann, ed.( Cold Spring Harbor , NY : Cold Spring Harbor Laboratory Press), pp. 87-110.


11. Yamada, K., McCarty, D.M., Madden, V.J., and Walsh, C.E. (2003). Lentivirus Vector Purification Using Anion Exchange HPLC Leads to Improved Gene Transfer. Biotechniques 34:1074-1080.


What is the difference between Retro-, Lenti-, and Adeno- viruses?


Retrovirus: Classic, can integrate into the genome but with low transduction efficiency. They are useful for gene transfer and protein expression in cells that have low transfection efficiency with other transfection reagents.

Lentivirus: Can integrate into the genome with relatively high transduction efficiency and they are very useful for cells that have low transfection efficiency with other transfection reagents. No special competent cells required, as they are stable plasmids. Lentiviruses are a powerful tool for stable gene transfer to both dividing and non-dividing cells in vitro and in vivo.

Adenovirus: Only work transiently (about 7 days) but have almost 100% transduction efficiency. Adenoviruses can infect a broad range of cell types with the highest efficiency and infection is not dependent on active host cell division. A second key feature is that high virus titers and high-level gene expression can be obtained in most mammalian cells.


What are the correct concentration units for each recombinant viral particle?


For lentiviruses and retroviruses, they are measured in CFU/ml (colony-forming units per millilitre). Transduction with lentiviruses and retroviruses can cause the formation of colonies, which can be quantified for concentration. Adenoviruses are measured as PFU/ml (plaque-forming units per millilitre). Transduction with adenoviruses will kill packaging cells, forming plaques in the process for quantification. The concentration for all three types of viruses can also be classified as IU/ml (Infectious Units/ml). Ultimately, the units refers to the viral particles and different units reflect the different assays involved.


Both recombinant retrovirus and lentivirus are widely used in the transduction of a varity of target cells and can be produced by transient transfection, from which viral supernatant can be collectedand used for target cell transduction. The titer of viral supernatant by transient transfection is approximately 106 cfu (colony forming unit)/ml, which is sufficient Purification Experimental Flow Chart for the generation of most stable cell lines in vitro. However, there are applications that require higher purity and titers. These applications include experiments demanding higher gene transduction efficiency and in vivo gene delivery. In addition, crude viral supernatants are not suitable for in vivo administration due to various contaminants contained in cell culture supernatant. Thus, the viral supernatant needs to be further concentrated and purified before use.
  Traditionally, both recombinant retrovirus and lentivirus have been concentrated via ultracentrifugation. Although the method works well, it requires specific ultracentrifugation equipment and it is technically demanding. In addition, the total viral supernatant volume is limited to the size of ultracentrifugation tubes. Reports have also stated that the ultracentrifugation process has some detrimental physical effects on the recombinant virus.
  Due to limitations on the existing technique, there is great necessity for a quick and efficient method to purify and concentrate recombinant retrovirus and lentivirus. Scientists at ABM Inc. have had years of experience with Ion-Exchange-based filter membranes and have successfully developed PuRetro™, the most effective retroviral and lentiviral purification method based on this technology.


Advantages of PuRetro™ Lentivirus Purification Kits


·        easy to use


·        very reliable


·        very affordable


 


 

太鼎生物科技 發表在 痞客邦 留言(0) 人氣()


Western Q 之簡易操作流程 (高速西方點墨反應儀) WTQ101


高速西方點墨反應儀 Western Q 跟傳統的方式比較,時間從原有的4小時縮短至40分。不論是定量表現、再現性及靈敏度,測試結果都與原先傳統的方法相同或更具優勢。

業務專員 許 虹宜 0920312382










   データシート
   ※ 4℃ o/n の抗体もご使用可能です











高速免疫染色装置 Western Q® は、従来振とうで4時間以上かかっていたウエスタンブロッティングの免疫染色の工程*を、わずか40分に短縮します。





















  従来法と同等以上のイメージ / 高い定量性・再現性・感度

  

    従来法

    240 分
 
Western Q
循環パルス法(A)
40 分
 
Western Q
循環パルス法(B)
40 分


定量性、再現性、感度といった結果のクオリティは従来法と同等以上です。


タンパク質がブロッティングされたメンブレンを、ブロッキングに始まり、一次抗体反応、二次抗体反応、洗浄する全工程。



A. 裝置架設


 


1) 運轉功能確認


首先連接機體與電源供應器,並準備一廢液瓶。將排放管開口置入廢液瓶中,並加入適量二次水於機體上方拖盤內。打開電源,按下邦浦啟動鍵(綠色),檢查二次水是否經邦浦吸入且經排放管流入廢液瓶中。此時可同時檢查液體流速是否可藉邦浦速度調整鈕進行調整,以及按下脈衝按鈕(黃色)確認邦浦是否可間些性做動。


2) 安裝membrane


首先請先將孔狀金屬板及尼龍網片以二次水沾濕,並由下往上依序將孔狀金屬板及尼龍網片置於機體上方拖盤內,將以清洗溶液沾濕之membrane以蛋白質面朝上之方向放置於尼龍網片中央,再將適當開口(75x 60m m)之矽膠蓋片放置於membrane之上。將排放管開口置入廢液瓶中,按下邦浦啟動鍵(綠色)以去除多餘之溶液。此時在確認溶液排空後矽膠蓋片是否緊密吸附於尼龍網片上。若確認正常,最後加入數滴清洗溶液並觀察溶液是否可完全經membrane上之孔洞快速流出。


3) 安裝排放管


如圖所示,將支撐器插入機後方,並將排放管固定於支撐器上,使排放管末段超出支撐器卡榫。


B. 免疫染色流程


1) 封鎖(Blocking, 5分鐘)


首先將邦浦速度調整鈕設定至2,並且加入8毫升封鎖溶液在membrane上,按下邦浦啟動鍵使溶液進行循環反應,並確認此一循環反應過程中封鎖溶液能覆蓋整張membrane5分鐘後將排放管開口置入廢液瓶中,以去除多餘之封鎖溶液。


2) 一級抗體反應(15分鐘)


首先按下脈衝按鈕並將邦浦速度調整鈕設定至2,並且加入8毫升一級抗體溶液在membrane上,按下邦浦啟動鍵使溶液進行脈衝式循環反應,15分鐘後將排放管開口置入廢液瓶中,去除多餘之一級抗體溶液(若需重複使用一級抗體溶液,只需將排放管開口置入乾淨離心管中進行回收即可)。


3) 二級抗體反應(15分鐘)


如同上述一級抗體反應步驟,加入8毫升二級抗體溶液在membrane上,按下邦浦啟動鍵及脈衝按鈕使溶液進行脈衝式循環反應,15分鐘後將排放管開口置入廢液瓶中,去除多餘之二級抗體溶液。


4) 清洗(Washing, 5分鐘)


將排放管開口置入廢液瓶中並移除機身上之支撐器,將清洗套件放置於矽膠蓋片上,並加壓使其黏附於矽膠蓋片上。將邦浦速度調整鈕設定至3,並且緩慢加入100毫升清洗溶液在membrane上,在所有清洗溶液流出機體至廢液瓶後將membrane取出浸泡於清洗溶液中等待訊號偵測。













  高速免疫染色装置 Western Q® の特徴






 
























 


1. Immunostaining by Western Q Circulated Pulse Method (Standard Protocol)The following data are immunostaining of western blotting using various antibodies as primary antibodies. Two times
dilution series of total protein from 10mg to 0.625mg derived from IGF-1 stimulated (50ng/ml, 5minutes) mouse cultured cell, P19, was blotted on PVDF membrane. Shaking in antibody solution was conducted for traditional method, and Circulated Pulse Method was conducted for Western Q. Same concentrations of antibodies (recommendedby manufacturers) were used for traditional shaking method and for Western Q Circulated Pulse Method. A) β-Actin (C4) (Santa Cruz) 1/3000 dilution Traditional Method: 4 hours Western Q Circulated PulseMethod: 40minutes exposure: 40 seconds exposure: 40 seconds
Traditional Method: Blocking 1hour (5% skim milk), Primary antibody1hour, Secondary antibody 1hour, Washing total 60 minutes, Total 4 hoursWestern Q Standard Protocol: Blocking 5 minutes (0.5% skim milk), Primary antibody 15minutes, Secondary antibody 15minutes, Washing 5 minutes, Total 40minutes
Secondary antibody: Polyclonal goat anti-mouse immunoglobulins/HRP (Dako), 1/7500 dilutionECL (GE Healthcare) was used for chemiluminescence reaction. Exposed on X ray film
B) Phospho-p44/42 MAPK (Thr202/Tyr204) (E10) Mouse mAb
Cell Signaling 1/2000 dilution
4℃ overnight reaction is recommended by the manufacturer
Traditional Method: Primary antibody 16 hours Western Q CirculatedPulse Method: 40 minutes exposure 2.5 minutes exposure 10 minutes
Traditional Method: Blocking 1hour (5% skim milk), Primary antibody16 hours, Secondary antibody 1hour, Washing total 60 minutes, Total18 hours Western Q Standard Protocol: Blocking 5 minutes (0.5% skim milk), Primary antibody 15minutes, Secondary antibody 15minutes,Washing 5 minutes, Total 40minutes
Secondary antibody: Polyclonal goat anti-mouse immunoglobulins/HRP (Dako), 1/7500dilution ECL Plus (GE Healthcare) was used for chemiluminescence reaction. Exposed on X ray film C) Phospho-Akt (Ser473) (Cell Signaling) 1/1000 dilution 4℃ overnight reaction is recommended by the manufacturer Traditional Method: Primary antibody 16 hours Western Q Circulated Pulse Method: 85 minutes
exposure: 5 minutes exposure: 15 minutes Traditional Method: Blocking 1hour (5% skim milk), Primary antibody 16 hours ( 4℃ ), Secondaryantibody 1hour, Washing total 60 minutes, Total 18 hours
Western Q modified protocol: Blocking 5 minutes (0.5% skim milk), Primary antibody 60 minutes, Secondary antibody 15minutes, Washing 5 minutes, Total 85minutes Secondary antibody: Polyclonal swine anti-rabbit IgG/HRP (Dako), 1/7500dilution ECL Plus (GE Healthcare) was used for chemiluminescence reaction. Exposed on X ray film
Reaction speed by Western Q (Circulated Pulse Method) is several todozen times higher than one by traditional shaking method.
Protocol adjustment such as increasing time or raising concentration may be necessary for immunostaining which requires overnight reaction by traditional method because of antibodies or samples



 


 





簡単操作 40分
標準プロトコール(循環パルス法)で40分の操作で免疫染色が完了します。抗体濃度・プロトコールによっては30分程度に短縮可能です。振とうでオーバーナイトが必要な抗体では、40分以上かかることがあります。
用途に合わせた2種類のプロトコール
高感度ハイクオリティを実現する「循環パルス法」と、複数の抗体反応を簡易的に行うための「インキュベーション法」の2種類の基本プロトコールを準備しました。
ハイクオリティな結果
循環パルス法では最適化されたフローコントロールにより、高い定量性・再現性・感度を実現します。
 →  詳細は データシート をご参照ください。
抗体濃度の最適化不要
抗体濃度は基本的に従来法と同様で結構です。多くの場合、抗体メーカーの推奨濃度で反応できます。 必要な溶液量はワイドゲル(140×80mm)15ml、ミニゲル(80×80mm)8ml 、80×40mmでは4mlとなります。
ゲルに合わせたコンパクトボディ
狭いスペースでも利用でき、移動も楽に行えます。
 
 製品リーフレット(PDF) のダウンロード
 
 ご利用者の感想 もご覧いただけます。 
 
 Click to download  Leaflet on Western Q
 
  and  Datasheet on Western Q
 


























   従来法と同等以上のイメージ / 高い定量性・再現性・感度

  

    従来法

    240 分
 
Western Q
循環パルス法(A)
40 分
 
Western Q
循環パルス法(B)
40 分



  • lane1~5: ヒト培養細胞由来全タンパクを10μgから0.625μgまで2倍希釈系列をブロッティング
  • ブロッキング: 従来法は5%スキムミルク、Western Q 循環パルス法は0.5%スキムミルク使用


  • 一次抗体: Mouse monoclonal β-Actin (C4) (Santa Cruz)、1/3000希釈
  • 二次抗体: Polyclonal goat anti-mouse immunogloblins/HRP (Dako)、1/7500希釈
  • ECL(GE Healthcare)で反応させた後、X線フィルムに40秒間露光
   同じ条件での比較


  • Western Q 循環パルス法はA, B 2回の反復実験






   Western Q® を用いた高速免疫染色の原理



Western Q を用いた方法では、抗体溶液をメンブレンの微細孔内部に継続的に流し通過させることにより抗原と抗体の会合を促進します。さらにパルスフローにより分子の安定的な結合を促進し、短時間で高いシグナル強度を実現します。ポンプにより溶液を循環させるため一定量の抗体溶液で抗原抗体反応を効率よく行うことが可能になりました。






   循環パルス法を用いた標準操作



  1. メンブレンをセットし、メンブレンカバーを重ね合わせます。

     
     
  2. ブロッキング溶液 8ml をメンブレン上に満たし、5 分間循環させた後、排出。

     
     
  3. 一次抗体溶液を 8ml メンブレン上に満たし、15 分間パルスフローで循環させた後、排出。

     
     
  4. 二次抗体溶液 8ml を加え、15分間パルスフローで循環させた後、排出。
  5. 洗浄ユニットをセットし、洗浄溶液を 100ml 加え、すぐ排出。(約5分)
  6. メンブレンを取り出し、軽く洗浄溶液で濯ぎ、化学発光試薬で検出。
    簡単操作、40分!







   Western Q® 仕様

 

























型式WTQ101 (ミニゲル対応)WTQ102 (ワイドゲル対応)
外形寸法(チューブ固定具装着時)150(W)×150(D)×157(H) mm190(W)×150(D)×157(H) mm
重量1.6 kg1.7 kg
最大メンブレンサイズ90×90 mm140×90 mm
必要抗体溶液量8ml (90×90mmメンブレン)15ml (140×90mmメンブレン)
ポンプ速度5~40ml/分

太鼎生物科技 發表在 痞客邦 留言(0) 人氣()



萃取gDNA很簡單


傳統萃取 “尾巴gDNA”,無法直接PCR;因組織內含有PCR反應抑制因子,抑制PCR反應。須經由有機溶劑或kit萃取gDNA


專利研發DirectPCR Lysis Reagents具有抑制PCR抑制物的反應,只要一個步驟反應,即可將DNA由組織中釋放,


並且不須經由任何純化的步驟,溶液可以直接PCR,進行genotyping分析。


最經濟的萃取試劑, 500次尾巴, 只須6250  (0.5cm尾巴加入200ul為例                                                                                                                                   活動期間: 100630日止


Genotyping基因型鑑定」是全面搜尋人類致病基因的重要分析技術。DirectPCR DNA Extraction System,可以  快速有效萃取動物組織, 尾巴基因型DNA (genomic DNA),不須經由Phenol/Chloroform萃取步驟, 不須再通過column  等繁瑣步驟純化,只要一個步驟,效率100%。如此方便快速的方法, 可以應用於尾巴genomic DNA萃取、細胞 genomic DNA萃取、耳朵genomic DNA萃取、卵黃囊genomic DNA萃取。


更多資料:  http://www.viagenbiotech.com/
























DirectPCR DNA Extraction Reagents
Genotyping without DNA isolation





Description:  Viagen DirectPCR® DNA Extraction System is a single-tube system for rapid preparation of DNA from mouse tails, ear pieces, yolk sac, and culture cells.  The patent-pending components developed by scientists at Viagen Biotech Inc. allow that the resulting DNA extracts are compatible with genomic PCR for genotyping.  (more)


Simplified procedure:
1.  Lyse tails in DirectPCR reagents.
2.  Incubate for 45 min at 85°C.
3.  PCR using 1 µl lysates.


Detailed protocols:  Tail, Ear, Yolk Sac, Cultured cells.


Representative PCR results


DirectPCR Products:  Tail, Ear, Yolk Sac, Cultured cells.  


New !! Proteinase K Solution : For a small number of tails, genomic PCR quality..



  



 



 



Order / Prices
































































DirectPCR DNA Extraction Reagents Cat# Comments  
DirectPCR Lysis Reagent (mouse tail) [101-T] 250 mouse tails (50 ml)   
DirectPCR Lysis Reagent (mouse tail) [102-T] 500 mouse tails (100 ml)
DirectPCR Lysis Reagent (yolk sac) [201-Y] yolk sacs (50 ml)
DirectPCR Lysis Reagent (yolk sac) [202-Y] yolk sacs (100 ml)  
DirectPCR Lysis Reagent (cell) [301-C] cultured cells (50 ml)  
DirectPCR Lysis Reagent (cell) [302-C] cultured cells (100 ml)
DirectPCR Lysis Reagent (mouse ear) [401-E] mouse ear (25 ml)
DirectPCR Lysis Reagent (mouse ear) [402-E] mouse ear (50 ml)
Proteinase K Solution [501-PK] US and Canada only  


 


若有任何產品上的問題, 歡迎來信或來電洽詢!!


太鼎生技虹宜敬上


 


誠摯的心為您服務


祝您實驗順利 心想事成


 




















Hung-Yi Hsu



 許虹宜



Product Specialist



產品專員



BioPioneer Inc.



太鼎生物科技有限公司



TEL: (02)8660-9496



FAX: (02)8660-9342



Cell: 0920312382


更多代理產品請參考:



 


Http://www.biopioneer.com.tw




太鼎生物科技 發表在 痞客邦 留言(0) 人氣()


細胞

細胞外受質---integrin蛋白質

            



Abmgood公司integrin蛋白質與抗體產品【台灣總代理-太鼎生物科技有限公司02-86609496】


 



細胞表面有許多受體,與其結合的物質(ligand)可分為可溶與不可溶於水兩類。與不可溶物質結合的受體對細胞彼此之附著相當重要。有些受體可與其他表面之類似受體結合(如cadherins),也有一類受體和胞外基質(extracellular matrix)結合,可使細胞嵌合在體內正確位置,而integrin便屬於後者,因此我們在此形容 integrin 為細胞外基質的受器。integrins是由α、β次單元膜蛋白組合而成,α、β次單元各為一個蛋白質,各擁有一個穿透膜的domainsingle-trans membrane domain),雙體併成為integrins。其α、β次單元各有許多同型體 (isoforms),可以組合成至少22種不同integrins作為細胞外基質的受體。它們與細胞外基質的蛋白質結合,而在細胞內的部分則與許多種細胞結構相關連性的蛋白質結合。當其與細胞外基質結合後,它們會彼此聚集,與一些細胞內蛋白質結合,共同形成一個附著點被稱為--focal adhesion 集中附著點,集中附著點為一巨大蛋白質複合物(見下圖,只顯示出細胞內蛋白質分子)。細胞外基質包括膠原蛋白質(collagens)及延展蛋白質(elastins),與細胞外蛋白醣大分子(proteoglycans),與附著性分子(例如fibronectins laminins)結合,附著性分子與其受器結合,integrins 即為附著性分子之受器。


 


引用資料來自: http://vschool.scu.edu.tw/biology/content/cytology/%E7%B4%B0%E8%83%9E%E5%A4%96%E5%9F%BA%E8%B3%AA%E7%9A%84%E5%8F%97%E5%99%A8.htm


由圖示我們可以看到integrin利用其β次單元使一些蛋白質的複合物聚集在一起,並決定了這些蛋白質和其後續引發之訊號的專一性。β次單元可以直接結合在細胞內的talin及α-actinintalin是集中附著點的主要構造蛋白質,並與actinvinculinFAK結合。α-actinin是一個可將微絲(microfilamentG-actin組成) 兩兩 作聯結(cross- linked)的蛋白質,α-actinin並可與vinculin分子結合。vinculin集中附著點的含量最高的構造蛋白質,透過α-actinintalin結合上 integrinvinculin亦可與tensin和α-actin相結合;如此integrin和微絲細胞骨骼就連接在一起了。


      那麼integrin與訊號傳遞之關係又如何呢?FAK(focal adhesion kinase,一種非受體型的tyrosine kinase ,便在此過程扮演要角。FAK不具任何SH2SH3 domains (SH2 domain for binding to phospho- tyrosine; SH3 domain for binding to proline-rich regions)FAK如何能在不具這些domains的情況下固定在聚焦吸附處呢?實際上FAK是由其C端與talinpaxillin結合。一旦固定在該處後,FAK會在其第397個氨基酸tyrosine上自我磷酸化,此磷酸化之tyrosine便可與一非受體型之tyrosine kinase Src上之 SH2 domain結合。此時Src便可磷酸化FAK上三處胺基酸,其中之一便做為Grb2SH2 domain之結合位。FAK上具有一些富含proline的區域,而這些區域便可與p130 CAS上之SH3 domain結合。p130CAS又可與其他一些蛋白質結合(諸如CrkNck),這些蛋白質便可以引發細胞內一些後續的訊號。此外,p130CAS本身之tyrosine則可被FAK-Src複合物磷酸化,做為與上述蛋白質之結合位。Src除了會對FAKp130CAS進行磷酸化外,對paxillin tensin 也有相同功用。這兩種結構蛋白都可能參與了聚焦吸附的調控。


 目前知道Ras-MARK反應路徑可以是需要或不需要FAK的,而integrins是否會活化Ras呢?研究顯示integrins α-次單元位於胞外的區域可與其他膜上蛋白質結合,此蛋白質又會結合到Shc上。Shc上某些tyrosine若被磷酸化,其便可以將Grb2-SOS複合物帶至膜上並活化Ras


 


ECM → integrins (plasma membrane) → FAK/Src


 


FAK/Src → PI 3-K → Akt → Cell survival


FAK/Src → Grb2/Sos → Ras → Erk → Gene expression / Cell Proliferation 


FAK/Src → Cas → Crk/C 3G → Rap 1? Cytoskeleton changes


FAK/Src → Paxillin → Crk ? → Rac → migration


FAK/Src → Paxillin → Crk ? → Rac → JNK → Gene expression 


FAK/Src CSK


      上述這些訊號傳遞作用和細胞週期之進行具有什麼相關呢?基本上細胞週期是由三件事調控,即生長刺激因子(mitogens 例如growth factorscytokines )、與其他細胞之接觸(contact with other cells )、固著於基質層(anchorage to a substratum )。細胞週期之進行需要生長刺激因子,即這類訊號會使細胞進入細胞週期。然而細胞週期是受接觸抑制的(contact inhibtion),所以當空間太小時,細胞便不會進入細胞週期。此外細胞也必須固著至某個基質層(例如透過ECM),而integrin之重要性即在於此---它們調控了細胞週期之固著依存性(anchorage-dependence)。由上所述我們知道 mitogens integrins會一起協同調控細胞週期之進行,單獨一項是無法成功達成的。如果我們同時將細胞置於ECM上並給予生長因子(一種mitogens),細胞週期便會啟動。然而這對癌細胞而言又如何呢?癌細胞生長常為不依存生長刺激因子及基質層固著。


Reference:


Vuori, K. (1998). Journal of Membrane Biology 165: 191-199.


 


 

 


 


 



To order, check the corresponding box(es) on the right, and then click 'Add to Cart'.



表單的頂端

























































































































































Product



Size



Concentration



Cat. No.



Integrin β3 (Phospho-Tyr773) Antibody



100ug



1ug/ul



Y011060



Integrin β3 (phospho-Tyr785) Antibody



100ug



1ug/ul



Y011282



Integrin β3 (Ab-773) Antibody



100ug



1ug/ul



Y021082



Integrin β3 (Ab-785) Antibody



100ug



100ug/100ul



Y021274



Anti-ADAM17(a disintegrin and metalloproteinase domain 17 isoform 1 preproprotein)



100ug



1ug/ul



Y050477



Anti-ADAMTS-1(A disintegrin and metalloproteinase with thrombospondin motifs 1)



100ug



1ug/ul



Y050478



Anti-ITGA5(Integrin alpha-5)



100ug



1ug/ul



Y050579



Anti-ITGA2(Integrin alpha-2)



100ug



1ug/ul



Y050735



Anti-ITGAV(Integrin alpha-V)



100ug



1ug/ul



Y050790



Anti- ADAM-8 (A Disintegrin And Metalloproteinase-8, CD156, MS2)



100μg



1ug/ul



Y051488



Anti-ADAM-8 (A Disintegrin And Metalloproteinase-8, CD156, MS2)



100μg



1ug/ul



Y051489



Anti-ADAM-8 (A Disintegrin And Metalloproteinase-8, CD156, MS2)



100μg



1ug/ul



Y051490



Anti-ADAM-13 (A Disintegrin And Metalloproteinase-13, X-ADAM-13); Propeptide domain



100μg



1ug/ul



Y051501



Anti-ADAM-13 (A Disintegrin And Metalloproteinase-13, X-ADAM-13); Cytoplasmic domain



100μg



1ug/ul



Y051502



Anti-ADAM-17 (A Disintegrin And Metalloproteinase-17, TNF-a Converting Enzyme, TACE) ; Propeptide domain



100μg



1ug/ul



Y051506



Anti-ADAM-17 (A Disintegrin And Metalloproteinase-17, TNF-a Converting Enzyme, TACE) ; Cytoplasmic domain



100μg



1ug/ul



Y051507



Anti-ADAM-17 (A Disintegrin And Metalloproteinase-17, TNF-a Converting Enzyme, TACE) ; Activation site



100μg



1ug/ul



Y051508



Anti-ADAM-17 (A Disintegrin And Metalloproteinase-17, TNF- Converting Enzyme, TACE), Amino end of catalytic domain



100μg



1ug/ul



Y051509



Anti-ADAM-29 (A Disintegrin And Metalloproteinase-29); Cytoplasmic domain



100μg



1ug/ul



Y051515



Anti-ADAM-29 (A Disintegrin And Metalloproteinase-29); Propeptide domain



100μg



1ug/ul



Y051516



Anti-ADAM-33 (A Disintegrin And Metalloproteinase-33); Propeptide domain



100μg



1ug/ul



Y051517



Anti-ADAM-33 (A Disintegrin And Metalloproteinase-33); Aminoterminal end



100μg



1ug/ul



Y051518



Anti-ADAM-33 (A Disintegrin And Metalloproteinase-33); Cytoplasmic domain



100μg



1ug/ul



Y051519



Anti-ADAMTS-9 (A Disintegrin And Metalloproteinase with ThromboSpondin motif #9); Propeptide domain



100μg



1ug/ul



Y051533



Anti-ADAMTS-9 (A Disintegrin And Metalloproteinase with ThromboSpondin motif #9); spacer between TS1 and TS2



100μg



1ug/ul



Y051534



Anti-ADAMTS-9 (A Disintegrin And Metalloproteinase with ThromboSpondin motif #9); between first and second furin site



100μg



1ug/ul



Y051535



Anti-ADAMTS-9 (A Disintegrin And Metalloproteinase with ThromboSpondin motif #9); carboxy end of propeptide domain



100μg



1ug/ul



Y051536



Rat Anti-Mouse CD18/Integrin β2-UNLB



100ug



100ug/200ul



Y053723



太鼎生物科技 發表在 痞客邦 留言(0) 人氣()


 

Fc receptors是一種glycoproteins, 存在於血液中B細胞、單核細胞、及顆粒細胞。與抗體中Fc region具高親和力,造成非專一性及背景值形成 去除所有非專一性及背景值的問題 針對血液、骨髓或淋巴組織最適用!



 


www.innovexbiosciences.com


Fc Receptor Blocker


 



innovex biosciences公司Fc Receptor blocker【台灣總代理-太鼎生物科技有限公司】 02-86609496


 



 


 


 


 


 


 


 


 


 


 


 


 


 


ADVANCED and UNIQUE FEATURES:
• 30 minute incubation step at room temperature
• Blocks Fc Receptors in both human and animal cells
• Eliminates false positive staining of white blood cells, lymphoid tissues, cytosmears (blood & bone marrow)
• Insures accurate lymphoma and leukemia typing
• Eliminates negative control staining of lymphomas, lymphoid tissues, and blood and bone marrow smears
• Eliminates false positive staining for Kappa and Lambda staining
• Eliminates false positive staining of Reed Sternberg cells
• A must for CD markers staining in immunohistochemistry, immunofluorescence
• A must for CD phenotyping via flow cytometry
• A must for Immunoglobulins (Igs) staining via immunohistochemistry, flow cytometry and immunofluorescence
• A must for kappa and lambda staining


 


Innovex Fc Receptor Blocker does not contain any antibodies, immunoglobulins or immunoglobulin fragments 

Now also available in azide-free for live cell/functional assays
Azide-Free Fc Blocker can be used on live cells and for functional assays without cytotoxicity
Ordering info, scroll down



Innovex Fc Receptor Block is a peerless technology designed to block Fc Receptors present on all leukocytes (white blood cells), lymphomas, and melanomas. Fc receptors are also expressed on a majority of tumors. Blocking Fc receptors is essential for accurate typing of lymphoid and tumor tissues and cells. Fc receptor staining occurs by the binding of Fc receptors present on cell to the Fc region of the primary and/or secondary antibody. Fc Receptor Block can be used for IHC (frozen and paraffin) flow cytometry, immunoflorescence and in-situ hybridization and for live cell functional assays.



Fc Receptor Blocker is a must for accurate lymphoma and leukemia typing.



ADVANCED and UNIQUE FEATURES:
• 30 minute incubation step at room temperature
• Blocks Fc Receptors in both human and animal cells
• Eliminates false positive staining of white blood cells, lymphoid tissues, cytosmears (blood & bone marrow)
• Insures accurate lymphoma and leukemia typing
• Eliminates negative control staining of lymphomas, lymphoid tissues, and blood and bone marrow smears
• Eliminates false positive staining for Kappa and Lambda staining
• Eliminates false positive staining of Reed Sternberg cells
• A must for CD markers staining in immunohistochemistry, immunofluorescence
• A must for CD phenotyping via flow cytometry
• A must for Immunoglobulins (Igs) staining via immunohistochemistry, flow cytometry and immunofluorescence
• A must for kappa and lambda staining




 


Please click this icon to download the data sheet in PDF file.


 








































Item# Size# of SlidesShelf life
Buy

NB309

60 ml

700-1000

3 yrs


NB309-15

15 ml

150 - 250

3 yrs



NB335 (NEW)

30 ml

Azide - Free

1 yr

     

 



 


太鼎生物科技 發表在 痞客邦 留言(0) 人氣()



SouthernBiotech公司高品質二抗【台灣總代理-太鼎生物科技有限公司02-86609496】


Southernbiotech高品質二抗

































































































































































































































































































































<

太鼎生物科技 發表在 痞客邦 留言(0) 人氣()

 



6100-01Goat Anti-Chicken IgG(H+L)-UNLBIgG1.0 mg
6100-02Goat Anti-Chicken IgG(H+L)-FITCIgG1.0 mg
6100-04Goat Anti-Chicken IgG(H+L)-APIgG1.0 mL
6100-05Goat Anti-Chicken IgG(H+L)-HRPIgG1.0 mL
6100-08Goat Anti-Chicken IgG(H+L)-BIOTIgG1.0 mg
6100-09Goat Anti-Chicken IgG(H+L)-PEIgG0.5 mg
6110-01Goat Anti-Turkey IgG(H+L)-UNLBIgG1.0 mg
6110-02Goat Anti-Turkey IgG(H+L)-FITCIgG1.0 mg
6110-04Goat Anti-Turkey IgG(H+L)-APIgG1.0 mL
6110-05Goat Anti-Turkey IgG(H+L)-HRPIgG1.0 mL
6110-08Goat Anti-Turkey IgG(H+L)-BIOTIgG1.0 mg
6110-09Goat Anti-Turkey IgG(H+L)-PEIgG0.5 mg
8200-01Mouse Anti-Chicken CD3-UNLBIgG10.5 mg
8200-02Mouse Anti-Chicken CD3-FITCIgG10.5 mg
8200-08Mouse Anti-Chicken CD3-BIOTIgG10.5 mg
8200-09Mouse Anti-Chicken CD3-PEIgG10.1 mg
8200-13Mouse Anti-Chicken CD3-SPRDIgG10.1 mg
8210-01Mouse Anti-Chicken CD4-UNLBIgG10.5 mg
8210-02Mouse Anti-Chicken CD4-FITCIgG10.5 mg
8210-08Mouse Anti-Chicken CD4-BIOTIgG10.5 mg
8210-09Mouse Anti-Chicken CD4-PEIgG10.1 mg
8220-01Mouse Anti-Chicken CD8a-UNLBIgG10.5 mg
8220-02Mouse Anti-Chicken CD8a-FITCIgG10.5 mg
8220-08Mouse Anti-Chicken CD8a-BIOTIgG10.5 mg
8220-09Mouse Anti-Chicken CD8a-PEIgG10.1 mg
8220-15Mouse Anti-Chicken CD8a-CY5IgG10.1 mg
8230-01Mouse Anti-Chicken TCRgd-UNLBIgG10.5 mg
8230-02Mouse Anti-Chicken TCRgd-FITCIgG10.5 mg
8230-08Mouse Anti-Chicken TCRgd-BIOTIgG10.5 mg
8230-09Mouse Anti-Chicken TCRgd-PEIgG10.1 mg
8240-01Mouse Anti-Chicken TCRab/Vb1-UNLBIgG10.5 mg
8240-02Mouse Anti-Chicken TCRab/Vb1-FITCIgG10.5 mg
8240-08Mouse Anti-Chicken TCRab/Vb1-BIOTIgG10.5 mg
8240-09Mouse Anti-Chicken TCRab/Vb1-PEIgG10.1 mg
8250-01Mouse Anti-Chicken TCRab/Vb2-UNLBIgG10.5 mg
8250-02Mouse Anti-Chicken TCRab/Vb2-FITCIgG10.5 mg
8250-08Mouse Anti-Chicken TCRab/Vb2-BIOTIgG10.5 mg
8250-09Mouse Anti-Chicken TCRab/Vb2-PEIgG10.1 mg








8255-02Mouse Anti-Chicken CD4-FITCIgM0.5 mg
8255-08Mouse Anti-Chicken CD4-BIOTIgM0.5 mg
8255-09Mouse Anti-Chicken CD4-PEIgM0.1 mg
8260-01Mouse Anti-Chicken CD28-UNLBIgG10.5 mg
8260-02Mouse Anti-Chicken CD28-FITCIgG10.5 mg
8260-08Mouse Anti-Chicken CD28-BIOTIgG10.5 mg
8260-09Mouse Anti-Chicken CD28-PEIgG10.1 mg
8270-01Mouse Anti-Chicken CD45-UNLBIgM0.5 mg
8270-02Mouse Anti-Chicken CD45-FITCIgM0.5 mg
8270-08Mouse Anti-Chicken CD45-BIOTIgM0.5 mg
8270-09Mouse Anti-Chicken CD45-PEIgM0.1 mg
8270-11Mouse Anti-Chicken CD45-APCIgM0.1 mg
8270-13Mouse Anti-Chicken CD45-SPRDIgM0.1 mg
8280-01Mouse Anti-Chicken CD8b-UNLBIgG2a0.5 mg
8280-02Mouse Anti-Chicken CD8b-FITCIgG2a0.5 mg
8280-08Mouse Anti-Chicken CD8b-BIOTIgG2a0.5 mg












ApoSENSOR™ ATP Cell Viability Assay Kit

【細胞活性ATP分析系列產品】

ATP冷光細胞活性分析套組 【太鼎生物科技有限公司02-86609496】BIOVISION台灣代理經銷商




Catalog#: K254-200  | Size: 200 assays
 

 















Product Details


Kit Summary:
• Detection method- Luminometer or Beta Counter.
• Sample type- Cell and Tissue lysates, culture media, urine, soil, sludge, plasma and serum, as well as many other biological fluids
• Species reactivity- Mammalian
• Kit size- 200, 1000 assays
• Applications- Bioluminescent detection of the ATP level via luciferase catalyzed reaction for a rapid screening of apoptosis and cell viability in mammalian cells.


Features & Benefits:
• Simple one-step procedure; takes only 30 minutes
• Fast and convenient
• The assay can be done directly in culture plates requiring no harvest/washing/or sample preparations. The assay can be fully automatic for high throughput (10 seconds/sample) and is highly sensitive (detects 10-100 mammalian cells/well).


Kit components:
• Nucleotide Releasing Buffer
• ATP Monitoring Enzyme
• Enzyme Reconstitution Buffer
• ATP (MW 551)


Description:
Cell death (especially apoptosis) is an energy-dependent process that requires ATP. As ATP levels fall to a point where the cell can no longer perform basic metabolic functions, the cell will die. A typical apoptotic cell exhibits a significant decrease in ATP level. Therefore, loss of ATP level in cell has been used as an indicator of cell death. In contrast, cell proliferation has been recognized by increased levels of ATP. The ApoSENSOR™ Cell Viability Assay Kit utilizes bioluminescent detection of the ATP levels for a rapid screening of apoptosis and cell proliferation simultaneously in mammalian cells. The assay utilizes luciferase to catalyze the formation of light from ATP and luciferin, and the light can be measured using a luminometer or Beta Counter. The assay can be fully automatic for high throughput (10 seconds/sample) and is extremely sensitive (detects 10-100 mammalian cells/well). The high sensitivity of this assay has led to many other applications for detecting ATP production in various enzymatic reactions, as well as for detecting low level bacterial contamination in samples such as blood, milk, urine, soil, and sludge.


Storage Conditions:
-70°C


Shipping Conditions:
gel pack


USAGE: For Research Use Only! Not For Use in Humans.


其它相關產品









































7902-100 



 StayBrite™ D-Luciferin, Sodium Salt  



 100 mg 



 7902 -1G  



 StayBrite™ D-Luciferin, Sodium Salt  



  1 g  



 7902 -10G  



 StayBrite™ D-Luciferin, Sodium Salt  



  10 g  



 K791-100 



 StayBrite™ Highly Stable ATP Assay Kit  



 100 assays 



 K791-1000 



 StayBrite™ Highly Stable ATP Assay Kit  



 1000 assays 



 7901-150 



 StayBrite™ Highly Stable Luciferase  



 150 ug 



 K790-100 



 StayBrite™ Highly Stable Luciferase/Luciferin Reagent  



 100 assays 



 K790-1000 



 StayBrite™ Highly Stable Luciferase/Luciferin Reagent  



 1000 assays 



 K790-10000 



 StayBrite™ Highly Stable Luciferase/Luciferin Reagent  



 10000 assays 



太鼎生物科技 發表在 痞客邦 留言(0) 人氣()

【太鼎生物科技有限公司02-86609496


<如何以肉眼就能判讀細菌污染>coming soon


























Check ThreeTM NEO






































Unscrew a tube out in the direction of an arrow.Use a swab to take a sample by wiping or moistening.
Avoid a swab contact with any part other than the specimen attentively.
Screw and snap-on a tube tightly in the direction of an arrow till medium falls into a tube.
 
After confirmation of medium-fallen into a test tube, incubate it with incubator for 24 hours at 35C. Once incubation is over, interpret it visually by color alteration with following the table of criteria.After assessment, rotate a cap quarterly (90°) in the direction of the arrow.
  


















  
  
Thrust the cap tightly into the tube to drop the disinfecting solution into the test tube.Shake the test tube to mix the disinfecting solution with the culture solution. Let it rest for approximately 5 minutes and dispose of it.  




















細菌污染檢驗筆
其主要特點:


1.      不須額外購買任何儀器設備, 不須昂貴的細菌培養箱, 就可自行培養細菌



2.      可以偵測五種常見的細菌污染


3.      肉眼即可判讀是否有細菌污染


 








Check ThreeTM NEO   (Non-IVD)




















































  Check ThreeTM NEO is a simple bacterial test device to detect the presence of bacteria that may cause food poisoning, determined by color alteration.
Check ThreeTM NEO is widely used to voluntary inspection of causative organisms “food poisoning” and/or sanitary monitoring and control in the facility.
Check ThreeTM NEO can be performed by anyone with swiftness and precision for sanitary indicator.
You’ve got to try Check ThreeTM NEO. It should contribute to manage / education at your facility.
  
 Check ThreeTM NEO is available in package units of 30 tests for the detection of:
 
 1. Coliform group
 2. Staphylococcus aureus
 3. Salmonella
 4. Vibrio
 5. Coliform group (for lacto-)*
 *Specimen which contains milk solid ingredients, such as Soft-serve dairy ice cream, dairy ice-shake drink.
  
 













 1. 2. 3. 4. 5.
 
  Further details
\  


太鼎生物科技 發表在 痞客邦 留言(0) 人氣()


TdTBrdu方法,專業品質值得信賴­—Tunel assays



業務專員 許 虹宜 0920312382



TACS•XL® In Situ Apoptosis Detection Kits


      TACS•XL®原位凋亡檢測試劑盒,使用bromodeoxyuridine (BrdU),經TdT作用加入凋亡細胞DNA碎片的3’ 端。這與使用生物素或地高辛標記核苷酸的Tunel方法相比,效率更高。通過使用biotin conjugated anti-BrdU antibodystreptavidin-HRP,提高了細胞內染色的信噪比。試劑盒分為:TACS•XL® DABTACS•XL® Blue LabelTACS•XL® Basic三種規格。


 


試劑盒組成:


 



特點:


信號強、背景低


較生物素和地高辛標記檢測,假陽性率更低


• DAB TACS Blue Label™ 可供選擇


獨有的Cytonin™試劑,提高染色的透化作用


• TACS-Nuclease™試劑,供陽性對照片製作


可與螢光檢測試劑配套使用


應用:


石蠟或冰凍切片原位凋亡檢測


有助於凋亡形態學鑒別


儲存: 不同組分-20°C4°C 分別儲存


參考文獻:


1. Murray, N., L.A. Davidson, R.S. Chapkin, W.C. Gustafson, D.G. Schattenberg, and A.P. Fields. 1999. Overexpression of protein kinase C II induces colonic hyperproliferation and increased sensitivity to colon carcinogenesis. J. Cell. Biol. 45:699-711.


2. Bank, N., M. Kiroycheva, P.C. Singhal, G.M. Anthony, G.J. Southan, and C. Szabo. 2000. Inhibition of nitric oxide synthase ameliorates cellular injury in sickle cell mouse kidneys. Kidney Int. 58(1):82-89.


3. Li, X. and Z. Darzynkiewicz. 1995. Labelling DNA strand breaks with BrdUTP. Detection of apoptosis and cell proliferation. Cell Prolif. 28:571-579.


4. Gavrieli. Y., Y. Sherman, and S.A. Ben-Sasson. 1992. Identification of programmed cell death in situ via specific labeling of nuclear DNA fragmentation. J. Cell. Biol. 119:493-501.


 


相關產品:
























































貨號



 產品說明



規格



 4828-30-DK



 TACS.XL DAB Kit



 30 Samples



 4828-30-BK



 TACS.XL Blue Labeling Kit



 30 Samples



 4828-30-K



 TACS.XL Kit



 30 Samples



 4828-30-R



 TACS.XL Replenisher Kit



 30 Samples



 4828-30-AC



 TACS.XL Antibody Module



 30 Samples



 4828-30-BC



 TACS Blue Label Detection Module



 30 Samples



 4828-30-DC



 TACS.XL DAB Detection Module



 30 Samples



 4828-30-N



 TACS.XL Nuclease Module



 15 Samples



 4828-30-04



 TACS B-dNTP Mix



 30 µl 



 4828-30-06



 anti-BrdU antibody



 30 µl



 4828-30-12



 Strep-Diluent



 7.5 ml 



 4828-30-18



 Methyl Green



 50 ml



 


 


 


獨有的陽離子優化系統、Cytonin™透化技術—Tunel assays更清晰


 


TACS™ 2 TdT In Situ Apoptosis Detection Kits


       The TACS™ 2 TdT Kits採用Trevigen所獨有的陽離子優化系統增強了對特殊組織的標記能力。選用高度純化的TdT enzyme,配以生物素化核苷酸,可供DAB TACS Blue Label™顯色或熒光檢測。


 


試劑盒組成:


 


 



特點:


• TdT酶原位標記


陽離子優化系統,信號強,背景低


• DABTACS Blue Label™或熒光檢測可供選擇


獨有的Cytonin™試劑,提高染色的透化作用


• TACS-Nuclease™試劑,供陽性對照片製作


 


應用:


石蠟或冰凍切片原位凋亡檢測


光學顯微鏡


螢光顯微鏡


流式細胞檢測


 


儲存: 不同組分-20°C4°C 、室溫分別儲存


 


參考文獻:


1. Cooper, L.F., J.C. Tiffee, J.P. Griffin, H. Hamano, and Z. Guo. 2000. Estrogen-induced resistance to osteoblast apoptosis is associated with increased hsp27 expression. J. Cell Physiology 185:401-407.


2. Kasahara, Y., R. Tuder, L. Taraseviciene-Stewart, T. LeCras, S. Abman, P. Hirth, J. Waltenberger, and N. Voelkel. 2000. Inhibition of VEGF receptors causes lung cell apoptosis and emphysema. J. Clin. Inves. 106:1311-1319.


3. Gavrieli, Y., Y. Sherman, and S.A. Ben-Sasson. 1992. Identification of programmed cell death in situ via specific labeling of nuclear DNA fragmentation. J. Cell. Biol. 119:493-501.


4. Lovelace, C.I.P., J. Zhang, P.G. Vanek, and G.B. Collier. 1996. Detecting apoptotic cells in situ. Biomedical Products 21:76-77.


 


相關產品:












































































 貨號



 產品說明 



規格 



 4810-30-CI



 Cations (1 of each)



 30 µl each 



 4810-30-CK



 TACS 2 TdT-Core Kit



 30 Samples



 4810-30-K



 TACS 2 TdT-DAB Kit



 30 Samples



 4810-30-R



 TdT 2 Replenisher Kit



 30 Samples



 4811-30-K



 TACS 2 TdT-Blue Label Kit



 30 Samples



 4812-30-K



 TACS 2 TdT-Fluorescein Kit



 30 Samples



 4810-30-02



 TACS 2 TdT Labeling Buffer



 100 ml



 4810-30-03



 TACS 2 TdT Stop Buffer



 100 ml



 4810-30-04



 TACS 2 TdT dNTP



 30 µl



 4810-30-05



 TdT Enzyme



 30 µl



 4810-30-09



 Colbalt Cation (Sold as 4810-90-09)



 30 µl



 4810-30-10



 Magnesium Cation



 30 µl



 4810-30-14



 Manganese Cation (Sold as 4810-90-14)



 30 µl



 4810-90-09



 Cobalt Cation



 3 x  30 µl



 4810-90-10



 Magnesium Cation



 3 x  30 µl



 4810-90-14



 Manganese Cation



 3 x  30 µl



  4876-05-01



 Cytonin



 5 ml



< body>

太鼎生物科技 發表在 痞客邦 留言(0) 人氣()

美國TREVIGEN公司成立於1992年,致力於研發偵測細胞凋零、DNA的斷損與修復,以及基因多樣性分析所需用到的抗體與試劑。

業務專員 許 虹宜 0920312382




細胞凋亡、損傷研究的利器


--Comet Assay(彗星試驗)FLARE檢測試劑盒


 


Comet Assay Single Cell Gel Electrophoresis


(彗星試驗/單細胞凝膠電泳,SCGE)


 


單細胞凝膠電泳(Single-Cell Gel Electrophoresis, SCGE)又稱彗星試驗(Comet Assay)為一項較新的電泳技術,自成功地用於檢測DNA單鏈斷裂以來,經不斷地改進,已成為一種快速、靈敏和簡便地檢測DNA損傷地方法,廣泛地應用於DNA放射損傷、DNA剪切損傷、DNA交聯的檢測、藥物的遺傳毒性評價和細胞凋亡鑒定等工作中。


 


原理:各種因素誘發細胞DNA損傷後會影響DNA的高級結構,使其超螺旋鬆散。這種細胞經過實驗中細胞原位裂解(堿裂解)DNA解鏈等過程後,電泳時損傷的DNA從核中溢出,朝陽極方向泳動,產生一個尾狀帶,未損傷的DNA部分則保持球形,二者共同形成 "彗星"。在一定範圍內,"彗星"的長度(代表DNA遷移距離)和經螢光染色或銀染色後,"彗星"螢光強度或光密度(代表DNA的量)DNA損傷程度相關,因此可以定量檢測單個細胞中的DNA損傷。


 


TREVIGEN公司提供以下三種CometAssay檢測試劑盒


 





































產品種類



CometAssay Kit


(螢光染色)



CometAssay Silver Staining Kit(銀染)



CometSlide™ HT


(高通量)



 



染料



SYBR® Green I核酸染料





SYBR® Green I核酸染料



檢測方式



螢光顯微鏡



光學顯微鏡



螢光顯微鏡



樣品處理數/玻片



2



2



20



優點



 



永久保存染色標本(SYBR® Green I螢光染料易淬滅)



快速、可靠地分析大量標本



共同特點



關鍵技術:獨有的、經特殊處理的CometSlide™玻片,可促使低熔點瓊脂糖粘附在玻片上,避免了傳統方法(需製備基底層瓊脂和預處理)的耗時和不可靠。因此具有以下優點:


1. 快速


2. 方便:將細胞加入低熔點Comet LMAgarose(瓊脂糖)-無需基底層或預處理,並吸移至CometSlide™玻片即可


3. 結果可靠


 


另外在每個CometSlide™玻片有一個疏水屏障,其有助於將細胞直接鋪在玻片上並可使用Trevigens DNA修復酶中的任何一種



應用



1. 檢測和定量DNA損傷


2.  快速、方便地鑒別凋亡細胞


3.  跟蹤DNA損傷



 


相關產品:

















































目 錄 號



產 品 名



規 格



4250-004-03



CometSlide



2



4250-050-03



CometSlide



25



4250-200-03



CometSlide



100



4250-050-K



CometAssay Kit



50 Samples



4254-200-K



CometAssay Silver Staining Kit(銀染試劑)



200 Samples



4251-050-K



CometAssay Silver Kit


(4254-200-K銀染試劑)



50 Samples



4252-040-01



CometAssay HT Slide



2



4252-040-K



CometAssay HT Sample Kit



40 samples



4252-200-01



CometAssay HT Slide



10 samples



4252-500-01



CometAssay HT Slide



25 samples



 


FLARE (Fragment Length Analysis using Repair Enzymes)Assay Kits


(使用修復酶的片段長度分析試劑盒)


用途:使用不同的DNA修復酶來檢測單個細胞中的DNA損傷類型


原理:與Comet Assay(彗星試驗)原理相同,不同點在於在檢測前加入DNA修復酶孵育來修復損傷的DNA,以確定DNA損傷類型。


用法:


誘導待檢測細胞損傷


將細胞浸埋在FLARE Slide(玻片)上的低熔點瓊脂糖內


裂解細胞並與DNA修復酶孵育


進行彗星電泳並分析


產品


TREVIGEN公司提供六種FLARE檢測試劑盒,分別含有六種DNA修復酶,即Fpg, Endonuclease III, hoGG1, T4-PDG, cv-PDGUVDE


每種FLARE檢測試劑盒分為完全試劑盒(FLARE Kit)和模組試劑盒(FLARE Module)兩種


FLARE Kit:包括所有檢測試劑及玻片,如修復酶、試劑、FLARE玻片及低熔點瓊脂糖等


FLARE Module:僅提供酶和緩衝液,無FLARE玻片和低熔點瓊脂糖等,因此需與TREVIGEN公司的Comet Assay Kit配合使用。


























































目錄號



產品名



所含DNA修復酶



所識別的損傷



4040-100-FK


 



E. coli Fpg.


FLARE Kit



Fpg(Formamidopyrimidine-DNAGlycosylase, 甲醯氨基嘧啶-DNA糖基化酶)



8-oxoguanine, DNA containing formamidopyrimidine moieties



4040-100-FM


 



E. coli Fpg


FLARE Module



4045-100-FK



E. coli


Endonuclease III FLAREKit



Endonuclease III(核酸內切酶III)



Thymine glycol, 5,6-dihydrothymine,urea, 5-hydroxy-6-hydrothymine,5,6-dihydrouracil, alloxan,5-hydroxy-5-hydrouracil,uracil glycol,5-hydroxycylosine,5-hydroxyuracil, thymine ring saturated or fragmentation product



4045-100-FM



E. coli


Endonuclease III FLARE Module



4130-100-FK



hoGG1 FLARE Kit



hoGG1(Human 8-oxo-Guanine DNA Glycosylase, 8-氧鳥嘌呤DNA糖基化酶)



8-oxoguanine, DNA containing formamidopyrimidine moieties



4130-100-FM



hoGG1


FLARE Module



4055-100-FK



T4-PDG FLARE Kit



T4-PDG(T4 phage pyrimidine dimer glycosylaseT4噬菌體嘧啶二聚體糖基化酶)



Cis-syn isomers of cyclobutone pyrimidine dimers



4055-100-FM



T4-PDG


FLARE Module



4065-100-FK



cv-PDG FLARE Kit



cv-PDG(chorella virus pyrimidine dimer glycosylase, chorella病毒嘧啶二聚體糖基化酶)



Cis-syn and trans-syn isomers of cyclobutone pyrimidine dimers



4065-100-FM



cv-PDG


FLARE Module



4100-100-FK



S. pombe UVDE


FLARE Kit



UVDE(Ultraviolet DNA Endonuclease,紫外線DNA核酸內切酶)



cyclobutone pyrimidine dimers,(6-4) photoproducts



4100-100-FM



S. pombe UVDE


FLARE Module



 


產品價格

太鼎生物科技 發表在 痞客邦 留言(0) 人氣()

 

【太鼎生物科技有限公司02-86609496】美國Trevigen台灣總代理

Trevigen BME2011年又發表文章了

Small Molecule Kinase Inhibitor Screen Identifies Polo-Like Kinase 1 as a Target for Neuroblastoma Tumor-Initiating Cells

Natalie Grinshtein, Alessandro Datti, Mayumi Fujitani, David Uehling, Michael Prakesch, Methvin Isaac, Meredith S. Irwin, Jeffrey L. Wrana, Rima Al-awar, and David R. Kaplan

Cancer Res; 71(4); 1385–95. (2011)

Ontario Institute for Cancer Research, and University of Perugia, Italy

The cancer cell behavior section is divided into five areas:      

 

Highlights:     

  • Exclusive, Quantitative Directed In Vivo Angiogenesis Assay (DIVAA).      
  • Pioneered unique Cell Invasion Optimization Assay System.    
  • Instituted PCR, MAP and RAP testing for more than 30 infectious agents on both.  Starting biological materials and final products.  
  • Lowered Endotoxin specification from 20 EU to 8 EU on BME.      

Data Posters (available upon request):     

Factors Affecting Basement Membrane Protein Dependent In Vitro Cell Models.      

  1. Tube Assay.
  2. Cell Invasion Assay.
  3. 3-D Culture Assays.  

 

Cultrex® Basement Membrane Extract and Specialty Proteins (mouse)

Catalog # Product Name Size
3430-001-01 Cultrex® BME with phenol red 1 ml
3430-005-01 Cultrex® BME with phenol red 5 ml
3430-50-06 Phenol Red Solution 50 µl
3431-001-01 Cultrex® BME with phenol red, reduced growth factor 1 ml
3431-005-01 Cultrex® BME with phenol red, reduced growth factor 5 ml
3432-001-01 Cultrex® BME, without phenol red 1 ml
3432-005-01 Cultrex® BME without phenol red 5 ml
3433-001-01 Cultrex® BME, without phenol red, reduced growth factor 1 ml
3433-005-01

Cultrex® BME, reduced growth factor without phenol red, PathClear®

5 ml

 

Cultrex® Basement Membrane Extract and Specialty Proteins (mouse)

Catalog # Product Name Size
3430-001-01 Cultrex® BME with phenol red 1 ml
3430-005-01 Cultrex® BME with phenol red 5 ml
3430-50-06 Phenol Red Solution 50 µl
3431-001-01 Cultrex® BME with phenol red, reduced growth factor 1 ml
3431-005-01 Cultrex® BME with phenol red, reduced growth factor 5 ml
3432-001-01 Cultrex® BME, without phenol red 1 ml
3432-005-01 Cultrex® BME without phenol red 5 ml
3433-001-01 Cultrex® BME, without phenol red, reduced growth factor 1 ml
3433-005-01 Cultrex® BME, reduced growth factor without phenol red, PathClear® 5 ml

 

【太鼎生物科技有限公司02-86609496

 

欲瞭解更詳細產品資訊,請登陸http://www.trevigen.com/

 

Cultrex® BME PathClear® (PC)

Catalog # Product Name Size
3430-001-02 Cultrex® BME with phenol red, PathClear® 1 ml
3430-005-02 Cultrex® BME with phenol red PathClear® 5 ml
3431-001-02 Cultrex® BMEwith phenol red, reduced growth factor, PathClear® 1 ml
3431-005-02 Cultrex® BME with phenol red, reduced growth factor, PathClear® 5 ml
3432-001-02 Cultrex®BME, without phenol red, PathClear® 1 ml
3432-005-02 Cultrex®BME, without phenol red, PathClear® 5 ml
3433-001-02 Cultrex® BME, without phenol red, reduced growth factor, PathClear® 1 ml
3433-005-02 Cultrex® BME, without phenol red reduced growth factor, PathClear® 5 ml

 

Cultrex® BME High Protein Concentration (HC20+)

Catalog # Product Name Size
3444-005-02 Cultrex® High Protein Concentration (HC20+) BME, PathClear® 5ml

 

Cultrex® BME Stem Cell Qualified

Catalog # Product Name Size
3434-001-02 Cultrex® Stem Cell Qualified, BME Growth Factor Reduced PathClear® 1 ml
3434-005-02 Cultrex® Stem Cell Qualified, BME Growth Factor Reduced PathClear® 5 ml

 

Cultrex® BME 3-D Culture™ Matrix

Catalog # Product Name Size
3445-001-01 3-D Culture Matrix 1 ml
3445-005-01 3-D Culture Matrix 5 ml

 

Cultrex® Basement Membrane Extract and Specialty Proteins (Human)

Catalog # Product Name Size
3415-001-02 Cultrex® Human BME PathClear® 1ml
3420-001-01 Cultrex® Human Fibronectin, PathClear® 1 mg
3421-001-01 Cultrex® Human Vitronectin, PathClear® 50 µg
3422-001-01 Cultrex® Human Vitronectin, Nucleic Acids Reduced, PathClear® 50 µg

太鼎生物科技 發表在 痞客邦 留言(0) 人氣()

1 2