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目前日期文章:201201 (2)

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分生專用培養基,方便您進行DNA Cloning、Yeast培養。
老品牌培養基LAB M,英國原裝進口,口碑好,其品質與DIFCO相當。採用鋁質密封封口膜,不易受潮,不會有摻其它原料之疑慮。品質保證、無潮解之風險。

 1. 微生物培養基 (粉末培養基, 500g )
2. 各式選擇性培養基 (粉末培養基, 500g )
3. 食品品管培養基 (粉末培養基, 500g )
4. 分子生物實培養基 (粉末培養基, 500g )
5. 各式培養基添加物 (粉末培養基, 500g )


 The introduction of HarlequinTM chromogenic media, the company´s development of ISO compliant media formulations for food industry testing, and the provision of services such as the SMART QA Proficiency Testing scheme have all confirmed Lab M´s leading position.

Lab M also supplies bulk peptones, extracts and other raw materials to pharmaceutical and large-scale fermentation industries, in compliance with current EDQM certification. Custom-designed and custom-blended products are also a speciality.

Lab M is certified in accordance with ISO 9001:2000 and ISO 13485; products for the clinical market are supplied in compliance with the European IVD directive and carry the CE mark.

太鼎生物科技有限公司 (02)86609496


原廠網站: www.labm.com

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Free Fatty Acid Quantification Kit
Price: 詢問許虹宜0920-312382
Catalog#: K612-100  | Size: 100 assays


()靈敏度高 (偵測長鏈游離脂肪酸)

脂肪酸氧化作用的循環,每經過一次循環中四個酵素的作用,就釋出一個兩碳的acetyl CoA衍生物,藉由螢光或是冷光儀可偵測長鏈脂肪酸的含量或脂肪酸氧化的程度.

() 應用範圍廣: 可直接應用在血清、血漿、 體液、食物、及培養液諸多種類等

() 操作方便:

(1) 準備標準曲線

(2) 將預測的sample (如液狀樣品: 血清 血漿體液 培養液)assay buffer調整至50ul/well

(3) 加入2ul ACS Reagent 標準液及sample 混合均勻後於室溫37度培養30分鐘

(4) 預混50 μl Reaction Mix, 並各別添加至ACS反應好的標準液及sample混合均勻後於室溫37 度培養30分鐘(避光)

(5) Measure O.D. 570 nm for colorimetric assay or fluorescence at

Ex/Em = 535/590 nm in a micro-plate reader.

(6)計算fatty acid含量


Published References:

1) Bobrovnikova-Marjon E et al (2008) PNAS; 105: 16314 - 16319.

Kit Summary:
• Detection method- Absorbance (570 nm) or Fluorescence (Ex/Em 535/587 nm)
• Sample type- Cell and Tissue culture supernatants, urine, plasma and serum, as well as many other biological fluids
• Species reactivity- Mammalian
• Application- The kit has a detection limit 2 µM free fatty acid in variety samples.

Features & Benefits:
• Simple procedure; takes ~40 minutes
• Fast and convenient
• Kit contains all necessary reagents for rapid, sensitive and accurate measurement of Adipolysis in cell culture samples

Kit components:
• Fatty Acid Assay Buffer
• Fatty Acid Probe (lyophilized)
• Dimethylsulfoxide (DMSO; Anhydrous)
• ACS Reagent
• Enzyme Mix
• Enhancer
• Palmitic Acid Standard (1nmol/µl)

Fatty Acids play very important roles in normal metabolism and many disease developments. They are precursors to a number of bioactive classes of compounds such as prostaglandins, leucotrienes and others, and have been implicated in diverse functions such as autism, immune system and inflammation response. BioVision’s Free Fatty Acid Quantification Kit provides a convenient, sensitive enzyme-based method for detecting the long-chain free fatty acids in various biological samples, such as serum, plasma and other body fluids, food, growth media, etc. In the assay, Fatty Acids are converted to their CoA derivatives, which are subsequently oxidized with the concomitant generation of color or fluorescence. C-8 (octanoate) and longer fatty acids can then be easily quantified by either colorimetric (spectrophotometry at λ = 570 nm) or fluorometric (at Ex/Em = 535/587 nm) methods with detection limit 2 µM free fatty acid in variety samples.

Storage Conditions:



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