DNA Fragmentation試劑套組-FITC 


ApoBrdU
DNA Fragmentation Assay Kit



Detects DNA
Fragmentation by FACS or FL (細胞)


原位觀察DNA Fragmentation (Brdu-FITC單株抗體)
靈敏度高於DIG方式。一個小時完成實驗,適用於懸浮或貼附型細胞,可應用螢光顯微鏡/流氏細胞儀。



Background Descriptions: Internucleosomal DNA fragmentation is a hallmark of apoptosis
in mammalian cells. BioVision’s Apo-BrdU In Situ DNA Fragmentation Assay Kit
provides complete components including positive and negative control cells for
conveniently detecting DNA fragmentation by fluorescence microscopy or flow
cytometry. The kit utilizes Br-dUTP (bromolated deoxyuridine triphosphate
nucleotides) which is more readily incorporated into DNA strand breaks than
other larger ligands (e.g., fluorescein, biotin or digoxigenin). The greater
incorporation gives rise to brighter signal when the Br-dUTP sites are
identified by a fluorescein labeled anti-BrdU monoclonal antibody.




產品貨號#k401-60(包括PCNC細胞)


 


產品介紹






Detection method- Flow cytometry (Ex/Em = 488/520 nm for FITC, and 488/623 nm
for PI) and fluorescence microscopy (FITC and rhodamine filters)

• Sample type- Cell culture (adherent and suspension), parafin embedded tissue
sections

• Species reactivity- Mammalian

• Applications- The TUNEL-based assay kit provides complete components
including positive and negative control cells for convenient detection of DNA
fragmentation in cultured cells and tissue sections.





Features
& Benefits:


• Simple procedure; takes ~ 1 hour

• Fast and convenient

• The assay is sensitive and stable

• The incorporation of BrdU in the procedure provides greater sensitivity than
biotinylated or digoxigenylated methods.





Kit
components:


• Positive Control Cells

• Negative Control Cells

• Wash Buffer

• Reaction Buffer

• TdT Enzymes

• Br-dUTP

• Rinse Buffer

• Anti-BrdU-FITC Antibody

• PI/RNase Staining Buffer










DNA Fragmentation相關產品連結

















































 Catalog# 



 Product Name 



 Size 



 



 



 3618-100 



 BrdU單株抗體, 100ug


Anti-BrdU
antibody (Clone B1G5) 
  


資料整理如下所示


http://tw.myblog.yahoo.com/taiding2000/article?mid=1150&prev=1151&next=1149



 100 µg 



 



 



 K401-60 



 BrdU分析套組 (BrdU-FITC抗體), 60個分析(附對照細胞)


適用懸浮型及貼附型細胞。Flow、顯微鏡螢光觀察。


ApoBrdU
DNA Fragmentation Assay Kit
  


資料整理如下所示


http://tw.myblog.yahoo.com/taiding2000/article?mid=1151&prev=1152&next=1150



 60 assays 



 



 



 K404-60 



BrdU分析套組 (BrdU-Red抗體), 60個分析(附對照細胞)


適用組織切片或福馬林固定之細胞。Flow、顯微鏡螢光 


ApoBrdU
Red DNA Fragmentation Kit
  


資料整理如下所示http://tw.myblog.yahoo.com/taiding2000/article?mid=1152&prev=-1&next=1151



 60 assays 



 



 



 K403-50 



BrdU分析套組 (BrdU-Biotin抗體), 50個分析(附對照細胞)


適用組織切片。顯微鏡觀察 


ApoBrdU-IHC
DNA Fragmentation Assay Kit
  


資料整理如下所示http://tw.myblog.yahoo.com/taiding2000/article?mid=1153&prev=1154&next=1152



 50 assays 



 



 



 K402-50 



BrdU分析套組 (FITC-dUTP), 50個分析(附對照細胞)


適用懸浮型及貼附型細胞。螢光顯微鏡觀察或Flow


ApoDIRECT
DNA Fragmentation Assay Kit
  


資料整理如下所示http://tw.myblog.yahoo.com/taiding2000/article?mid=1154&prev=-1&next=1153



 50 assays 



 



 









【連絡人】許虹宜 0920-312382 Hung-Yi Hsu





E-mailtaiding.biotech@msa.hinet.net





【公司電話】02-86609496【公司傳真】02-86609342





【相關網址】www.biopioneer.com





【公司網址】www.biopioneer.com.tw





 


 

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