太鼎生物科技/太鼎食安科技

Background Descriptions: Bromodeoxyuridine (5-bromo-2'-deoxyuridine, BrdU, BUdR, BrdUrd)
is a synthetic nucleoside that is an analogue of thymidine. The
immunocytochemical detection of bromodeoxyuridine (BrdU) incorporated into DNA
is a powerful tool to study the cytokinetics of normal and neoplastic cells. In
vitro or in vivo labeling of tumor cells with the thymidine analogue BrdU and
the subsequent detection of incorporated BrdU with specific anti-BrdU
monoclonal antibodies is an accurate and comprehensive method to quantitate the
degree of DNA-synthesis. BrdU is incorporated into the newly synthesized DNA of
S-phase cells may provide an estimate for the fraction of cells in S-phase.
Also dynamic proliferative information such as the S-phase transit rate and the
potential doubling time can be obtained, by means of bivariate BrdU/DNA flow
cytometric analysis. Antibody specific for BrdU is used to immunocytochemically
detect the incorporated BrdU, which is a powerful tool to study cell
proliferation, the cytokinetics of normal and neoplastic cells, etc.

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