Anti-H7N 9 HA蛋白質 (HA1+HA2)





40103-V08H4操作手冊


 Influenza
A H7N9 (A/Anhui/1/2013) HA / Hemagglutinin Protein Datasheet







H7N9
(A/Anhui/1/2013) HA / Hemagglutinin Protein




H7N9
(A/Anhui/1/2013) HA / Hemagglutinin Protein Product Information

























Synonym :




HA




Protein Construction:




A DNA sequence
encoding the Influenza A virus (A/Anhui/1/2013(H7N9)) hemagglutinin
(AGJ51953.1) (Met1-Val524) (cleavage between Arg339 and Gly440, HA1+HA2) was
expressed with a C-terminal polyhistidine tag.




Source:




Influenza A virus




Expression Host:




Human Cells








H7N9
(A/Anhui/1/2013) HA / Hemagglutinin Protein
QC Testing


































Purity:




(53.8+44.7) % as determined by SDS-PAGE




SDS-PAGE:

 



H7N 9 HA protein




Endotoxin:




< 1.0 EU per μg of the protein as determined by the
LAL method




Stability:




Samples are stable for up to twelve months from date of
receipt at -70




Predicted N terminal:




Asp 19




Molecular Mass:




The recombinant
hemagglutinin of Influenza A virus (A/Anhui/1/2013(H7N9)) comprises 517 amino
acids and has a predicted molecular mass of 57.6 kDa. The apparent molecular
mass of the protein is approximately 43 and 27 kDa in SDS-PAGE under reducing
conditions.




Formulation:




Lyophilized from sterile PBS, pH 7.4.




  1. Normally 5 % -
    8 % trehalose and mannitol are added as protectants before
    lyophilization. Specific concentrations are included in the hardcopy of
    COA.

  2. Please contact
    us
    for any concerns or special requirements.







H7N9
(A/Anhui/1/2013) HA / Hemagglutinin Amino Acid Sequence












DKICLGHHAV SNGTKVNTLT ERGVEVVNAT ETVERTNIPR ICSKGKRTVD
LGQCGLLGTI TGPPQCDQFL EFSADLIIER REGSDVCYPG KFVNEEALRQ ILRESGGIDK EAMGFTYSGI
RTNGATSACR RSGSSFYAEM KWLLSNTDNA AFPQMTKSYK NTRKSPALIV WGIHHSVSTA EQTKLYGSGN
KLVTVGSSNY QQSFVPSPGA RPQVNGLSGR IDFHWLMLNP NDTVTFSFNG AFIAPDRASF LRGKSMGIQS
GVQVDANCEG DCYHSGGTII SNLPFQNIDS RAVGKCPRYV KQRSLLLATG MKNVPEIPKG RGLFGAIAGF
IENGWEGLID GWYGFRHQNA QGEGTAADYK STQSAIDQIT GKLNRLIEKT NQQFELIDNE FNEVEKQIGN
VINWTRDSIT EVWSYNAELL VAMENQHTID LADSEMDKLY ERVKRQLREN AEEDGTGCFE IFHKCDDDCM
ASIRNNTYDH SKYREEAMQN RIQIDPVKLS SGYKDVAHHH HHHHHHH








H7N9
(A/Anhui/1/2013) HA / Hemagglutinin Protein Usage Guide

















Storage:




Store it under sterile conditions at -70 . It is recommended that
the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw
cycles
.




Reconstitution:




A hardcopy of COA with reconstitution instruction is
sent along with the products. Please refer to it for detailed information.








H7N9
(A/Anhui/1/2013) HA / Hemagglutinin Protein Related Products & Topics







Related Areas:





Proteins:





Antibodies:







H7N9
(A/Anhui/1/2013) HA / Hemagglutinin Protein Description







H7N9 is a subtype of Influenza
virus A. On April 1, 2013, the World Health Organization (WHO) first reported 3
human infections with a new influenza A (H7N9) virus in China . Since
then, additional cases have been reported. This new H7N9 virus is an avian
(bird) influenza (flu) virus. Influenza (flu) is a respiratory infection in
mammals and birds. The virus is divided into three main types (Influenza A, Influenza
B, and Influenza C), which are distinguished by differences in two major
internal proteins (hemagglutinin (HA) and neuraminidase (NA).





Influenza A is further divided
into subtypes based on differences in the membrane proteins hemagglutinin (HA)
and neuraminidase (NA), which are the most important targets for the immune
system. The notation HhNn is used to refer to the subtype comprising the hth
discovered Hemagglutinin (HA) protein and the nth discovered neuraminidase (NA)
protein. The influenza viral Hemagglutinin (HA) protein is a homo trimer with a
receptor binding pocket on the globular head of each monomer.





The influenza virus
Hemagglutinin (HA) protein is translated in cells as a single protein, HA0, or
hemagglutinin precursor protein. For viral activation, hemagglutinin precursor
protein (HA0) must be cleaved by a trypsin-like serine endoprotease at a
specific site, normally coded for by a single basic amino acid (usually
arginine) between the HA1 and HA2 domains of the protein. After cleavage, the
two disulfide-bonded protein domains produce the mature form of the protein
subunits as a prerequisite for the conformational change necessary for fusion
and hence viral infectivity.







References







1.                      
Barman S. et al., 2000, J Virol. 74: 6538-45.





2.                      
Suzuki T. et al., 2005, J Virol. 79: 11705-15.





3.                      
Shinya K. et al., 2006, Nature. 440 (7083): 435-6.





4.                      
Marjuki H. et al., 2006, J Biol Chem. 281: 16707-15.





5.                      
Christophe F. et al., 2009, Science. 324: 1557-61.





6.                      
Von Itzstein M. 2007, Nat Rev Drug Discov. 6: 967-74.





 


 

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