H6N 1 HA重組蛋白質





H6N1 (A/northern
shoveler/California/HKWF115/2007) HA Protein
重組蛋白質





產品型號及datasheet: 11723-V08H







H6N1 (A/northern
shoveler/California/HKWF115/2007) HA Protein Product Information

























Synonym :




HA




Protein Construction:




A DNA sequence encoding the
extracellular domain ( Met 1 - Gln 529 ) of the influenza A H6N1
hemagglutinin (A/northern shoveler/California/ HKWF115/2007 (H6N1) )
(ACE81692.1) (HA1+HA2, uncleaved) was expressed, fused with a C-terminal
polyhistidine tag




Source:




Influenza A H6N1 




Expression Host:




Human Cells








H6N1
(A/northern shoveler/California/HKWF115/2007) HA Protein QC
Testing


































Purity:




> 93 % as determined by SDS-PAGE




SDS-PAGE:

 



H6N 1 HA protein




Endotoxin:




< 1.0 EU per μg of the protein as
determined by the LAL method.




Stability:




Samples are stable for up to twelve
months from date of receipt at -70




Predicted N terminal:




Asp 17




Molecular Mass:




The
secreted recombinant influenza A H6N 1 HA ( A/northern shoveler/California/HKWF115/2007
(H6N1) ) comprises 524 amino acids and has a predicted molecular mass of 59.2
kDa. As a result of glycosylation, it migrates as an approximately 65 kDa
band in SDS-PAGE under reducing conditions.




Formulation:




Lyophilized
from sterile PBS , pH 7.4




  1. Normally 5 % - 8 % trehalose and mannitol are added as protectants
    before lyophilization. Specific concentrations are included in the
    hardcopy of COA.

  2. Please contact
    us
    for any concerns or special requirements.


 








H6N1
(A/northern shoveler/California/HKWF115/2007) HA Protein Usage Guide

















Storage:




Store it under sterile conditions at -70 . It is recommended that
the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw
cycles
.




Reconstitution:




A hardcopy of COA with reconstitution
instruction is sent along with the products. Please refer to it for detailed
information.








Related Influenza Virus Research Tools







·                         
Influenza Virus
Research Tools





·                         
Influenza
Hemagglutinin & Antibody





·                         
Influenza
Hemagglutinin Proteins





·                         
H6N1
Hemagglutinin & Antibody





·                         
A/northern
shoveler/California/HKWF115/2007







H6N1 (A/northern shoveler/California/HKWF115/2007) HA Protein Description







Influenza (flu)
is a respiratory infection in mammals and birds. This virus is divided into
three main types (A, B and C). Influenza A is found in a wide variety of bird
and mammal species and is further divided into subtypes based on differences in
the membrane proteins hemagglutinin (HA) and neuraminidase (NA).

H6N1 is a subtype of Influenza A. Hemagglutinin (HA) is a single-pass type
I  integral membrane glycoprotein from the influenza virus, and comprises
over 80% of the envelope proteins present in the virus particle. The HA is a
trimer with a receptor binding pocket on the globular head of each monomer. In
natural infection, inactive HA is matured into HA1 and HA2 outside the cell by
one or more trypsin-like, arginine-specific endoprotease secreted by the
bronchial epithelial cells. Binding of HA to sialic acid-containing receptors
on the surface of its target cell brings about the attachment of the virus
particle to the cell and forms a endosome. Low pH in endosomes induce an
irreversible conformational change in HA2, releasing the hydrophobic portion
“fusion peptide”. After which, virus penetrates the cell and pours its contents
including the RNA genome into the cytoplasm mediated by fusion of the
endocytosed virus particle’s own membrane and the endosomal membrane.
Hemagglutinin plays a major role in the determination of host range restriction
and virulence.







References







1.                      
Barman, S. et al., 2000, J. Virol. 74:
6538-45.





2.                      
Suzuki, T. et al., 2005, J. Virol. 79:
11705-15.





3.                      
Shinya K, et al., 2006,
Nature. 440 (7083): 435-6.





4.                      
Marjuki, H. et al., 2006, J. Biol.
Chem. 281: 16707-15.





5.                      
von, Itzstein, M. 2007, Nat. Rev. Drug.
Discov. 6: 967-74.





6.                      
Christophe F, et al., 2009, Science.
324:1557-61.





 


 


H6N1 流感抗體





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