H6N1
流感抗體





Influenza A H6N 1
HA / Hemagglutinin Antibody





產品型號及datasheet下載: 11723-MM04





可應用於WBELISA之分析





偵測靈敏度: WB (2 ng/lane)





ELISA( 0.039 ng/well
)







H6N1 Hemagglutinin
/ HA Antibody Product Information

































Immunogen :




Recombinant
H6N 1 HA
protein




Antibody Type :




Mouse Monoclonal Antibody ( Mouse mAb
Service Platform
)




Clone
ID
:




8A10D8




Ig Type :




Mouse
IgG1




Formulation :




0.2 μm filtered solution in PBS with
5% trehalose




Preparation :




This
antibody was produced from a hybridoma resulting from the fusion of a mouse
myeloma with B cells obtained from a mouse immunized with purified, human
cell-derived, recombinant Influenza A virus H6N1 Hemagglutinin. The IgG
fraction of the cell culture supernatant was purified by Protein A affinity
chromatography.








H6N1
Hemagglutinin / HA Antibody Usage Guide





























Specificity
:




H6N1
(A/northern shoveler/California/HKWF115/2007) HA




 




No
cross-reactivity
in ELISA with



H1N1
(A/California/07/2009) HA



H2N2
(A/Canada/720/2005) HA



H3N2
(A/Wyoming/03/2003) HA



H4N6
(A/mallard/Ohio/657/2002) HA



H5N1
(A/Anhui/1/2005) HA



H7N7
(A/chicken/Netherlands/1/03) HA



H8N4
(A/pintail duck/Alberta/114/1979) HA



H9N2
(A/Hong Kong/1073/99) HA



H10N3
(A/duck/Hong Kong/786/1979) HA



H11N2
(A/duck/Yangzhou/906/2002) HA



H12N5
(A/green-winged teal/ALB/199/1991) HA



H13N8
(A/black-headed gull/Netherlands/1/00) HA



H15N8
(A/duck/AUS/341/1983) HA



H16N3
(A/black-headed gull/Sweden/5/99) HA



Influenza
B (B/Florida/4/2006) HA




Western blot :




This antibody can be used at 1-2
μg/mL with the appropriate secondary reagents to detect H6N1-HA in WB. Using
a DAB detection system, the detection limit for H6N1-HA is approximately 4
ng/lane under non-reducing conditions and 2 ng/lane under reducing
conditions.




Direct ELISA :




This antibody can be used at 0.5-1
μg/mL with the appropriate secondary reagents to detect H6N1-HA. The
detection limit for H6N1-HA is approximately 0.039 ng/well.




Storage :




This antibody can be stored at 2 -8 for one month without
detectable loss of activity. Antibody products are stable for twelve months
from date of receipt when stored at -20 to -70 . Preservative-Free.

Sodium azide is recommended to avoid contamination (final concentration
0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid
repeated freeze-thaw cycles
.








Related Influenza Virus Research Tools







·                  
Influenza Virus
Research Tools





·                  
Influenza
Hemagglutinin & Antibody





·                  
Influenza
Hemagglutinin Proteins





·                  
H6N1
Hemagglutinin & Antibody





·                  
A/northern
shoveler/California/HKWF115/2007







H6N1 Hemagglutinin / HA Antibody Description







Influenza (flu) is a respiratory infection in mammals and
birds. This virus is divided into three main types (A, B and C). Influenza A is
found in a wide variety of bird and mammal species and is further divided into
subtypes based on differences in the membrane proteins hemagglutinin (HA) and
neuraminidase (NA).

H6N1 is a subtype of Influenza A. Hemagglutinin (HA) is a single-pass type
I  integral membrane glycoprotein from the influenza virus, and comprises
over 80% of the envelope proteins present in the virus particle. The HA is a
trimer with a receptor binding pocket on the globular head of each monomer. In
natural infection, inactive HA is matured into HA1 and HA2 outside the cell by
one or more trypsin-like, arginine-specific endoprotease secreted by the
bronchial epithelial cells. Binding of HA to sialic acid-containing receptors
on the surface of its target cell brings about the attachment of the virus
particle to the cell and forms a endosome. Low pH in endosomes induce an
irreversible conformational change in HA2, releasing the hydrophobic portion
“fusion peptide”. After which, virus penetrates the cell and pours its contents
including the RNA genome into the cytoplasm mediated by fusion of the
endocytosed virus particle’s own membrane and the endosomal membrane.
Hemagglutinin plays a major role in the determination of host range restriction
and virulence.







References







1.                      
Barman, S. et al., 2000, J. Virol. 74:
6538-45.





2.                      
Suzuki, T. et al., 2005, J. Virol. 79:
11705-15.





3.                      
Shinya K, et al., 2006,
Nature. 440 (7083): 435-6.





4.                      
Marjuki, H. et al., 2006, J. Biol.
Chem. 281: 16707-15.





5.                      
von, Itzstein, M. 2007, Nat. Rev. Drug.
Discov. 6: 967-74.




Christophe
F, et al., 2009, Science. 324:1557-61.


 


 


H6N 1
HA
重組蛋白質




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