人類71型腸病毒—VP0 (VP4-VP2)重組蛋白


訂購貨號#40013-V20B








 Human Enterovirus 71 VP0 ( VP4-VP2 )Protein























產品描述




VP0,  
VP4-VP2




蛋白質表達之架構




A DNA sequence
encoding the amino acids ( Met 1 - Gln 323 ) of human enterovirus 71 genome
polyprotein ( Q66478-1 ), corresponding to the protein VP0 precusor, a
component of immature procapsids, was fused with the N-terminal
polyhistidine-tagged GST tag at the N-terminus




Source:




Human Enterovirus 71




Expression Host:




Baculovirus-Insect cells








Human
EV-71 VP0 / VP4-VP2 Protein
QC Testing


































Purity:




> 72 % as determined by SDS-PAGE




SDS-PAGE:

 



VP0 protein




Endotoxin:




< 1.0 EU per μg of the protein as determined by the
LAL method




Stability:




Samples are stable for up to twelve months from date of
receipt at -70




Predicted N terminal:




Met




Molecular Mass:




The recombinant
human enterovirus 71 protein VP0 / GST chimera consists of 560 amino acids
and has a calculated molecular mass of 63 kDa. It migrates as an
approximately 58 kDa band in SDS-PAGE under reducing conditions.




Formulation:




Lyophilized from sterile 50 mM Tris, 100mM NaCl, 2 mM GSH, 0.5 mM PMSF,  pH 8.0




  1. Normally 5 % -
    8 % trehalose and mannitol are added as protectants before
    lyophilization. Specific concentrations are included in the hardcopy of
    COA.

  2. Please contact
    us
    for any concerns or special requirements.







Human
EV-71 VP0 / VP4-VP2 Protein Usage Guide

















Storage:




Store it under sterile conditions at -70 . It is recommended that
the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw
cycles
.




Reconstitution:




A hardcopy of COA with reconstitution instruction is
sent along with the products. Please refer to it for detailed information.








Human EV-71 VP0 / VP4-VP2 Protein Related Products & Topics







Related Areas:





Virus Research
Tools
>>VP0





Proteins:





Antibodies:







Human
EV-71 VP0 / VP4-VP2 Protein Description







Human enterovirus 71
genome polyprotein is a member of the picornaviruses polyprotein family. It
contains two peptidase C3 domains, one RdRp catalytic domain,
one SF3 helicase domain. Genome polyprotein is cleaved into the following
12 chains: Protein VP0 (VP4-VP2), Protein VP4 (P 1A ), Protein VP2 (P1B), Protein VP3 (P 1C ), Protein VP1 (P1D), Picornain 2A (P 2A ), Protein 2B (P2B), Protein 2C (P 2C ), Protein 3A
(P 3A ), Protein 3B (P3B),
Picornain 3C (Protease 3C ) and RNA-directed RNA polymerase 3D-POL
(P3D-POL). VP0 precursor is a component of immature procapsids. Capsid proteins
VP1, VP2, VP3 and VP4 form a closed capsid enclosing the viral positive strand
RNA genome. VP4 lies on the inner surface of the protein shell formed by VP1,
VP2 and VP3. All the three latter proteins contain a beta-sheet structure
called beta-barrel jelly roll. Together they form an icosahedral capsid
composed of 60 copies of each VP1, VP2, and VP3, with a diameter of
approximately 300 Angstroms. VP1 is situated at the 12 fivefold axes, whereas
VP2 and VP3 are located at the quasi-sixfold axes.







References







1.                     
Brown B.A., et al., 1995, Virus Res. 39:195-206.





2.                     
Tang W.-F. et al., 2007, J. Biol. Chem. 282:5888-5898.





3.                     
Huang, S.C.
et al.
, 2008, Virus Res. 131 (2):250-9.





 





 





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