A/Anhui/01/2005
H5N1 Hemagglutinin Protein Datasheet





 























HA Synonym:




HA




Protein Construction:




A
DNA sequence encoding the extracellular domain of Influenza A virus
(A/Anhui/1/2005(H5N1) (ABD28180.1) hemagglutinin(Met 1-Gln 527)with cleavage
site mutated (RERRRKR→TETR, HA1+HA2, uncleaved) was expressed with a
C-terminal polyhistidine tag.




Source:




Influenza A Virus H5N1




Expression Host:




Human Cells








A/Anhui/01/2005
H5N 1 HA Protein QC
Testing


































Purity:




>97% as determined by SDS-PAGE.




SDS-PAGE:
























11048-V08H1




Endotoxin:




< 1.0 EU per 1μg of the protein as
determined by the LAL method.




Stability:




Samples are stable for up to twelve
months from date of receipt at -70




Predicted N terminal:




Asp 17




Molecular Mass:




The secreted recombinant
hemagglutinin of Influenza A virus (A/Anhui/1/2005(H5N1) comprises 522 amino
acids with the predicted molecular mass of 59 kDa. As a result of
glycosylation, it migrates as an approximately 75 kDa band in SDS-PAGE under
reducing conditions.




Formulation:




Lyophilized
from sterile PBS , pH 7.4



1.   
Normally 5 % - 8 % trehalose and
mannitol are added as protectants before lyophilization. Specific
concentrations are included in the hardcopy of COA.



2.   
Please contact us
for any concerns or special requirements.








A/Anhui/01/2005
H5N 1 HA Protein Usage Guide

















Storage:




Store it under sterile conditions at -70 . It is recommended that
the protein be aliquoted and be used as soon as possible. Avoid repeated
freeze-thaw cycles.




Reconstitution:




A hardcopy of COA with
reconstitution instruction is sent along with the products. Please
refer to it for detailed information.








HA Protein Description







Influenza (flu)
is a respiratory infection in mammals and birds. This virus is divided into
three main types (A, B and C). Influenza A is found in a wide variety of bird
and mammal species. Influenza B is largely confined to humans and is an
important cause of morbidity. Influenza C infects humans, dogs and pigs,
sometimes causing both severe illness and local epidemics. Influenza A is
further divided into subtypes based on differences in the membrane proteins
hemagglutinin (HA) and neuraminidase (NA). The notation HhNn is used to refer
to the subtype comprising the hth discovered HA protein and the nth discovered
NA protein. The HA is a trimer with a receptor binding pocket on the globular
head of each monomer. Subtypes are further divided into strains. Each
genetically distinct virus isolate is usually considered to be a separate
strain.

Influenza A virus subtype H5N1, also known as "bird flu", A(H5N1) or
simply H5N1, is a subtype of the Influenza A virus which can cause illness in
humans and many other animal species. H5N1 is easily transmissible between
birds facilitating a potential global spread of H5N1. It is mainly spread by
domestic poultry, both through the movements of infected birds and poultry
products and through the use of infected poultry manure as fertilizer or feed.
Humans with H5N1 have typically caught it from chickens, which were in turn
infected by other poultry or waterfowl. Influenza H5N1 (A/Anhui/1/2005) virus
was isolated from a specimen of tracheal aspirate. The whole genome sequencing
indicated that all segments were of avian origin. The hemagglutinin receptor
binding site was similar to those of other avian H5N1 viruses, and a polybasic
amino acid cleavage site was present.







Related Influenza Virus Research Tools







·                         
Influenza Virus
Research Tools





·                         
Influenza
Hemagglutinin & Antibody





·                         
Influenza
Hemagglutinin Proteins





·                         
H5N1
Hemagglutinin & Antibody





·                         
H5N1
Hemagglutinin Proteins





·                         
A/Anhui/01/2005







A/Anhui/01/2005 H5N 1 HA
Protein Related Products & Topics







·                         
HA Protein Related Areas:





·                         
Proteins:





·                         
Antibodies:







References







1. Li KS, et
al., 2004, Nature. 430: 209-213.





2. van RD, et
al., 2006, Science. 312: 399.





3. Shinya K, et
al., 2006, Nature. 440 (7083): 435-436.





4. Taubenberger
JK, et al., 2008, Annu Rev Pathol. 3: 499-522.





5. Christophe
F, et al., 2009, Science. 324:1557-1561.





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