Anti-
IgG-Fc Antibody-HRP





 


Anti-IgG Antibody





Anti-human IgG (Fc) HRP Conjugated Antibody















Cat.No.:




Y052930




Quantity:




100 μl






 



































































Antibody Type




Primary antibody




Description




Anti-human IgG (Fc) HRP Conjugated is a Produced by hyperimmunizing the
host animal with highly purified human IgG (Fc) fraction. The goat IgG
fraction is prepared from antisera with a Protein G affinity column. The
polyclonal antibody is conjugated with horseradish peroxidase (HRP) using a
modification of the periodate procedure described by Wilson and Nakane1




Immunogen




Highly purified human IgG (Fc) fraction




Species Reactivity




Human, others not tested




Specificity




This antibody recognizes human IgG (Fc) fraction




Tested Applications




ELISA User Optimized




Application Notes




ELISA: For general use, the titer is 1:20,000, Direct ELISA using 0.5
ug/well coating dose of human IgG and TMB as substrate determined this titer.
The OD value of end point is 1.0 when the above dilution was used.




Raised In




Goat




Clonality




Polyclonal




Purity




Affinity purified




Storage Buffer




PBS, pH 7.4 with 0.05% sodium azide.




Formulation




Liquid




Concentration




1ug/ul




Storage




This product is stable for several weeks at 4° C as an undiluted liquid.
Dilute only prior to immediate use. For extended storage, aliquot contents
and freeze at -20° C or below. Avoid cycles of freezing and thawing.
Expiration date is one (1) year from date of receipt.




Caution




Anti-human IgG (Fc) HRP Conjugated is for research use only and is not
intended for therapeutic or diagnostic applications. Please contact a
technical service representative for more information.






 





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