【Yeast粒腺體萃取套組】Biovision訂購貨號#K259-50。Yeast Mitochondria Isolation Kit 分離高純度且完整之粒腺體蛋白質。 @ 太鼎生物科技/太鼎食安科技

Yeast
Mitochondria Isolation Kit

Catalog#: K259-50 | Size: 50 assays

Assay Kit Name:

Yeast Mitochondria Isolation Kit

Kit Summary:

Application:

• Isolation of highly pure intact mitochondria from yeast cells.

• Mitochondrial respiration studies, assembly of the complexes, apoptosis,
mtDNA and mtRNA, and for protein profiling.

• Western blot and ELISA

Sample Type:

• Yeast cells culture.

Features & Benefits:

• Simple & convenient protocol

• The kit provides unique formulations of ready-to-use buffers and reagents to
isolate intact mitochondria

Kit Components:

• Buffer A

• Buffer B

• 1 M DTT

• Homogenization Buffer

• Lysis Enzyme Mix

• Storage Buffer

• Protease Inhibitor Cocktail

Description:

Mitochondria are the power house of the cells because they generate most of the
supply of energy in the form of adenosine tri-phosphate (ATP). Mitochondria are
double membrane organelles: an outer membrane and a folded inner membrane
called cristae. Isolated mitochondria is a useful tool to study mitochondrial
respiration, assembly of the respiratory complexes, apoptosis, mtDNA and mtRNA,
and for protein profiling. BioVision’s Yeast Mitochondria Isolation kit will
enable fast and easy purification of mitochondria from yeast cells, utilizing
yeast cell wall lysis and homogenization.

This Mitochondria Isolation Kit is tested for Pichia pastoris &
Saccharomyces cerevisiae & can be used to isolate mitochondria from other
yeast. Under fermentable media, the yield is ~150-200 µg of mitochondria &
under non-fermentable media (e.g. Glycerol) ~200-250 µg of mitochondria from a
culture of OD ~20.