Mitochondrial
Protein IP Kit





Catalog#: K285-50 | Size: 50 assays





Assay Kit Name:

Mitochondrial Protein IP Kit





Kit Summary:

Application:

• Optimized for compatibility with immunoprecipitation (IP and co-IP) and
pull-down using tagged proteins.

• Gentle formulation for maintenance of stable mitochondrial complexes.

• Compatible with SDS PAGE, 2D gel, Blue-Native gel, Mass spectrometry.

• Functional Assays and enzymatic assays.





Sample Type:

• Mitochondria, mitochondrial extract or cell lysate.





Features & Benefits:

• Simple & convenient protocol

• The Kit provides unique formulation of ready-to-use buffers & reagents
for IP/Co-IP/Pull down of mitochondrial proteins or complexes.





Kit Components:

• Mitochondria Protein IP Buffer

• Wash Buffer (5X)

• Protease Inhibitor Cocktail

• 10% n-Dodecyl-beta-D-maltoside

• 10% Triton X-100

• 10% Digitonin




Description:


Mitochondria are the power house of the cells and play an essential role in
energy production. Damage to the mitochondria activates signaling pathways that
induce apoptosis. Mitochondria also regulate and mediate transport of the
metabolites and ions needed for oxidative phosphorylation and maintenance of
membrane potential for ATP synthesis. Mitochondrial dysfunction leads to
several disorders like cardiac dysfunction, diabetes, aging and neurological
disorder, mainly caused by mutations in mitochondrial DNA or in nuclear genes
that code for mitochondrial components. Thus, mitochondria have several
different functions in the cell.

BioVision ready to use mitochondria Protein IP Buffer is optimized for
immunoprecipitation (IP and co-IP) using mitochondria and mitochondrial
extracts. The buffer is a gentle formulation, which maintains the stability of
mitochondrial complexes. The Mitochondrial Protein IP kit is provided with
different choices of detergents like n-Dodecyl-beta-D-maltoside, Triton X-100
and digitonin to achieve different stringency conditions for protein-protein
interaction studies. Triton X-100 is the most commonly used detergent
especially for membrane protein solubilization. However, in case of fragile
complexes digitonin or n-Dodecyl-beta-D-maltoside is the choice of detergents.


【供應廠商】太鼎生物科技有限公司



【連絡人】許虹宜 0920-312382



【台北公司】02-86609496 【傳真】02-86609342



【相關網址】 www.biopioneer.com.tw



【原廠網址】 www.biovision.com


 

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