---病毒cDNA clone產品系列---


 

架構好之病毒cDNA Clone, 具有完整之序列報告。每一個cDNA經由最佳化序列設計改良, 在大部份的細胞株中, 具有最大量之蛋白質表現。





Influenza A H1N1
(A/California/07/2009) Hemagglutinin partial cDNA Clone / ORF Clone (codon
optimized)





訂貨資料VG11085-M











VG11085-M




Cloning
vector ($295)

pCMV / hygro ($495)

pCMV / hygro - HA ($495)

pCMV / hygro - His ($495)

pCMV / hygro - Myc ($495)

pCMV / hygro - FLAG ($495)






----H1N1 流感HA cDNA clone


產品資料------







Influenza A H1N1
(A/California/07/2009) Hemagglutinin cDNA Clone Product Information





















cDNA Size:




1617




Gene Synonym:




HA1, Hemagglutinin




Source:




Influenza Virus








Influenza A H1N1
(A/California/07/2009) Hemagglutinin cDNA Clone Description





















cDNA Description:




ORF Clone of Influenza A
H1N1 (A/California/07/2009) HA DNA.



This cDNA clone has gone
through customized codon optimization in order to obtain high level of
protein expression in particular cell lines. Therefore, although the
translated amino acid sequence is identical to the amino sequence on Gene
Bank, the DNA sequence is different from that on Gene Bank.




Vector:




Cloning Vector: pMD18-T Simple Vector.



Expression Vectors: pCMV/hygro,
pCMV/hygro-Myc, pCMV/hygro-His, pCMV/hygro-HA, pCMV/hygro-FLAG




His Tag Sequence:




CATCACCACCATCACCACCATCATCACCAT








Influenza A H1N1 (A/California/07/2009) Hemagglutinin
cDNA Clone Shipping







1.    5-10μg Plasmid DNA is shipped on Whatman FTA elute
card (Cat. No.: WB120410) at room temperature.





2.    It would take 7-14 days for delivery typically. For
bulk orders, additional 1-2 weeks would be needed.







Influenza A H1N1 (A/California/07/2009) Hemagglutinin
cDNA Clone Usage Protocol







1.    Cut along the circle edge of the spot (arrow points
to) to get the filter paper.





2.    Transfer the filter paper to a 1.5ml microfuge tube
containing 150-200 μl of sterile H2O . Ensure the filter paper is
completely immersed in the H2O by briefly centrifuging the tube for
10 seconds.





3.    Transfer the tube to incubation at 95 for 15-30 min.





4.    At the end of the incubation pulse vortex, or gently
tap the tube.





5.    Briefly centrifuge for 30 seconds, to separate the
scattered filter paper from the elute. The elute now contains the purified DNA.





架構之載體資料





Vector Sequence
Download


Description

Vector Name pCMV / hygro - Myc
Vector Size 5684bp
Vector Type Mammalian Expression Vector
Expression Method Constitutive, Stable / Transient
Promoter CMV
Antibiotic Resistance Ampicillin
Selection In Mammalian Cells Hygromycin
Protein Tag Myc
Sequencing Primer Forward:T7(TAATACGACTCACTATAGGG)
Reverse:BGH(TAGAAGGCACAGTCGAGG)


Schematic of pCMV / hygro - Myc Multiple Cloning Sites


Note: The Intrinsic signal peptide, if there is, will be used as default in construction.
Contact us if you would prefer to use the heterogeneous signal peptide (on the vector).


 


更多流感cDNA資料






http://www.sinobiological.com


 


 


【供應廠商】太鼎生物科技有限公司



【連絡人】許虹宜 0920-312382



【台北公司】02-86609496



【相關網址】 www.biopioneer.com.tw



【原廠網址】http://www.sinobiological.com

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