太鼎生物科技/太鼎食安科技

HRP抗體標誌套組

只需一個步驟

不須Dialysis或desalt步驟。效能近乎100%。

(貨號#701-0000)     

抗體標誌directly labeling kit，一定選擇英國innova biosciences公司專利研發之lightning-link套組。市面上antibody labeling kit，在pH8~9環境下做反應。如此地環境易造成抗體活性失效，即使照著原廠提供的Protocol下進行反應，抗體回收的效能也很差。因為多太抗體未被標誌，必須移去未結合的抗體。如此浪費珍貴的抗體。

英國INNOVA BIOSCIENCES，以生物結合為主要事業。於1996年專利研發，中性環境下進行抗體結合反應，只要一個步驟，30秒hands on time。其抗體回收效能近似100%。不須再做Dialysis或desalt的步驟。

Lightning-Link 只要一個步驟，即可將蛋白質/抗體/胜肽做標誌，具專利研發Quencher reagent化學反應徹底中止殘餘反應及停止非專一性結合，因此無須desalt及dialysis，抗體標誌回收率100%。

Lightning-link
抗體標誌原理:

Lightning-Link以共價鍵結，辨視amino
groups，性質極為穩定，一次反應後，可冷凍保存，節省實驗時間。

Lightning-link
抗體標誌V.S.保存液:

抗體保存於Azide、Gelatin及BSA溶液下，會干擾一般結合的效果，所以會再經過純化步驟，浪費許多抗體。Lightning-Link克服Azide、Gelatin及BSA干擾結合的效率，省下您寶貴的抗體。別於其它結合的方式，Lightning-Link無須在High pH的溶液下操作，所以只要是具有amino groups (lysin/a-amino)的蛋白質/胜肽/抗體，都可反應。

Lightning-link
抗體標誌應用:

可以應用在ELISA. immunofluorescent. Flow. confocol. Western
Blot等檢診及分子病毒之研究!!直接在一抗上做標誌 (可標誌螢光蛋白
螢光dye HRP Biotin等) 只要一個步驟 回收100%。這個方法克服二抗所造成的背景值過高及Host來源不同所造成Crosstalk的現象.

Lightning-link
產品種類:

抗體標誌產品，可分為五大類產品。(此部份僅介紹酵素型--抗體標誌產品)

1. 酵素型標誌產品；HRP、AP、Glucose
Oxidase等。

2. 螢光蛋白標誌產品；APC、PE、PerCP、RPE等螢光蛋白。

3. Biotin/Streptavidin標誌產品；Avidin、Streptavidin、Biotin等。

4. Tandem螢光標誌產品；應用FRET原理，使螢光emission於較遠的波段，例如APC/Cy5.5、PE/Cy7、PE/Cy5.5等。

Lightning-Link®
Alkaline Phosphatase (AP)

Lightning-Link®
Glucose Oxidase

Lightning-Link®
Horseradish Peroxidase (HRP)

Lightning-Link®
Avidin

Lightning-Link®
Biotin

Lightning-Link®
Streptavidin

Lightning-link
酵素型抗體標誌---期刊發表:

Lightning-Link
HRP  ---期刊發表

• Measurement of MMP-9 and -12 degraded elastin (ELM)
  provides unique
  
  information on lung tissue degradation - BMC Pulmonary
  Medicine (2012)


• The carboxy-terminus of TFPIα is required for its
  interaction with factor V and Va - Journal of Thrombosis and
  Haemostasis (2012)


• Development of monoclonal antibody-based immunoassays
  for detection of Helicobacter pylori neutrophil-activating protein
  - Journal of Immunological Methods (2012)


• Sensitive quantitative assays for tau and phospho-tau
  in transgenic mouse models - Neurobiology of Aging (2012)


• A Novel System for Rapid and Cost-Effective
  Production of Detection and Diagnostic Reagents of West Nile Virus in
  Plants - Journal of Biomedicine and Biotechnology (2012)


• Porcine Reproductive and Respiratory Syndrome Virus
  Activates the Transcription of Interferon alpha/beta (IFN-α/β) in
  Monocyte-derived Dendritic Cells (Mo-DC) - Veterinary
  Microbiology (2012)


• A Human PrM Antibody That Recognizes a Novel Cryptic
  Epitope on Dengue E Glycoprotein - PLoS One (2012)


• A Conserved Tandem Cyclophilin-Binding Site in
  Hepatitis C Virus Nonstructural Protein 5A Regulates Alisporivir
  Susceptibility - Journal of Virology (2012)

• Bacitracin inhibits the reductive activity of protein
  disulfide isomerase by disulfide bond formation with free cysteines in the
  substrate-binding domain - FEBS Journal (2011)


• Dual
  Neofunctionalization of a Rapidly Evolving Aquaporin-1 Paralog Resulted in
  Constrained and Relaxed Traits Controlling Channel Function during Meiosis
  Resumption in Teleosts - Mol Biol Evol (2011)


• Comparison of the efficiency of antibody selection
  from semi-synthetic scFv and non-immune Fab phage display libraries
  against protein targets for rapid development of diagnostic immunoassays
  - Journal of Immunological Methods (2011)


• Protein biomarkers in exfoliated cells collected from
  the human rectal mucosa: implications for colorectal disease detection and
  monitoring - International Journal of Colorectal Disease
  (2011)


• Assessment
  of proteolytic degradation of the basement membrane: a fragment of type IV
  collagen as a biochemical marker for liver fibrosis - Fibrogenesis
  & Tissue Repair (2011)


• Canine specific ELISA for coagulation factor VII
  - The Veterinary Journal (2011)


• Aberrant splicing of the hRasGRP4 transcript and
  decreased levels of this signaling protein in the peripheral blood
  mononuclear cells in a subset of patients with rheumatoid arthritis
  - PLoS One (2011)


• MMP Mediated Degradation of Type VI Collagen Is
  Highly Associated with Liver Fibrosis – Identification and Validation of a
  Novel Biochemical Marker Assay - PLoS One (2011)
  
  


• Ubiquitin Fold Modifier 1 (UFM1) and Its Target
  UFBP1 Protect Pancreatic Beta Cells from ER Stress-Induced Apoptosis - PLoS One (2011)


• A novel marker for assessment of liver matrix
  remodeling: An enzyme-linked immunosorbent assay (ELISA) detecting a MMP
  generated type I collagen neo-epitope (C1M) - Biomarkers
  (2011)


• Immunological detection of the type V collagen
  propeptide fragment, PVCP-1230, in connective tissue remodeling associated
  with liver fibrosis - Biomarkers (2011)


• Methodologic issues in the measurement of
  interleukin-16 in clinical blood samples using immunoassays -
  Cytokine (2011)


• Histidine-rich Glycoprotein Binds Fibrin(ogen) with
  High Affinity and Competes with Thrombin for Binding to the γ′-Chain
  - JBC (2011)

• Monoclonal antibody to novel cell surface epitope on
  Hsc70 promotes morphogenesis of bile ducts in newborn rat liver
  - Cell Stress and Chaperones (2010)


• Immunogenicity of attenuated measles virus engineered
  to express Helicobacter pylori neutrophil-activating protein
  - Vaccine (2010)


• Peptide Route Functionalization of ZnSe Crystals
  Preserves Activity and Structure of Proteins while Adsorption -
  The Journal of Physical Chemistry C (2010)


• Monoclonal antibody to novel cell surface epitope on
  Hsc70 promotes morphogenesis of bile ducts in newborn rat liver
  - Cell Stress and Chaperones (2010)


• Clathrin-independent
  carriers form a high capacity endocytic sorting system at the leading edge
  of migrating cells - JCB (2010)


• Regeneration of Dental-Pulp-like Tissue by
  Chemotaxis-Induced Cell Homing - Tissue Eng Part A. (2010)


• The Phosphatase Interactor NIPP1
  Regulates the Occupancy of the Histone Methyltransferase EZH2 at Polycomb
  Targets – Nucleic Acids Research (2010)

• Extinction-Reconsolidation
  Boundaries: Key to Persistent Attenuation of Fear Memories - Science
  (2009)


• Development
  of a highly sensitive ELISA for aldosterone in mouse urine: Validation in
  physiological and pathophysiological states of aldosterone excess and
  depletion - Steroids (2009)


• Contributions
  to Neutropenia from PFAAP5 (N4BP2L2), a Novel Protein Mediating
  Transcriptional Repressor Cooperation between Gfi1 and Neutrophil Elastase - Molecular and
  Cellular Biology (2009)

LifeXtend
HRP Conjugagte Stabilizer

• Sensitive quantitative assays for tau and phospho-tau
  in transgenic mouse models - Neurobiology of Aging (2012)

Lightning-Link
Biotin  ---期刊發表

• Controlled release of thymosin β4 from injected
  collagen–chitosan hydrogels promotes angiogenesis and prevents tissue loss
  after myocardial infarction - Regenerative Medicine (2012)


• Engrailed-2 (EN2): A Tumor
  Specific Urinary Biomarker for the Early Diagnosis of Prostate Cancer
  – Clinical Cancer Research (2011)


• Human Alpha Defensin 5 Expression in the Human Kidney
  and Urinary Tract - PLoS One (2011)


• Ribonuclease 7 is a potent antimicrobial peptide
  within the human urinary tract - Kidney International (2011)

• Method for Decreasing Immunogenicity ; Patent
  publication number US2011/0152505 (2010)


• Targeting
  MHC Class I Monomers to Dendritic Cells Inhibits the Indirect Pathway of
  Allorecognition and the Production of IgG Alloantibodies Leading to
  Long-Term Allograft Survival - Journal of Immunology (2010)


• Stromal
  Cell-Derived Factor-1α Activation of Tissue-Engineered Endothelial
  Progenitor Cell Matrix Enhances Ventricular Function After Myocardial
  Infarction by Inducing Neovasculogenesis - Cell Transplantation
  and Tissue Regeneration (2010)


• In Vivo
  Studies of Mutant Fibrillin-1 Microfibrils - JBC (2010)


• Serum
  insulin-like growth factor binding protein-1 (IGFBP-1) phosphorylation
  status in subjects with and without ischaemic heart disease - Artherosclerosis
  (2010)


• HER2 Phosphorylation Is
  Maintained by a PKB Negative Feedback Loop in Response to Anti-HER2
  Herceptin in Breast Cancer – PLoS Biology (2010)

• Human
  Mesenchymal Stem Cells Self-Renew and Differentiate According to a
  Deterministic Hierarchy – PLoS One (2009)


• Bovine
  lactoferrin and lactoferricin interfere with intracellular trafficking of
  Herpes simplex virus-1- Biochemie (2009)

• Functional studies of host-specific ephrin-B ligands
  as Henipavirus receptors - Virology (2008)

Lightning-Link
Avidin  ---期刊發表:

• Targeting MHC Class I Monomers
  to Dendritic Cells Inhibits the Indirect Pathway of Allorecognition and
  the Production of IgG Alloantibodies Leading to Long-Term Allograft
  Survival – Journal of Immunology (2010)

Lightning-Link
AP  ---期刊發表

• Large molecular size EDTA-resistant complexes
  containing S100A12, ERAC, in serum during inflammatory conditions
  - Scandinavian Journal of Clinical & Laboratory Investigation
  (2012)


• Broad-Spectrum Inhibition of HIV-1 by a
  Monoclonal Antibody Directed against a gp120-Induced Epitope of CD4 - PLoS One (2011)


• Fluorescent
  Labeling of Antibody Fragments Using Split GFP - PLoS One (2011)


• In Vivo
  Studies of Mutant Fibrillin-1 Microfibrils - JBC (2010)


• Microarray immunoassay for the detection of grapevine
  and tree fruit viruses - Journal of Virological Methods
  (2009)