【GST標誌蛋白質純化產品】Biovision, #6555-1GST-Tag親和層析蛋白質純化試劑, 只要經過Loading、Washing、Elute這三個步驟就可以得到一定純度以上的蛋白質。 @ 太鼎生物科技/太鼎食安科技

GST標誌重組蛋白純化

親和層析法為廣泛應用的蛋白質純化方法之一。使用His-Tag 或是GST-Tag 純化的流程相當簡單，只要經過Loading、Washing、Elute這三個步驟就可以得到一定純度以上的蛋白質。搭配FPLC 儀器進行純化，可獲得更高純度或更大量的重組蛋白。

【GST-Tag 純化原理】GST-Tag fusion protein 是使用glutathione S-transferase (GST)與glutathione 的親和特性（酵素和基質）進行重組蛋白質純化。Glutathione 在進行蛋白質純化前預先 coating 在Agarose beads 上作為glutathione-Agarose resin，當GST-fusion protein 通過glutathione-Agarose resin 時，GST-Tag 與glutathione 會產生親和性反應，並結合在glutathione coated resin 上，此時未含GST 的蛋白質會通過resin 並使用wash buffer 達到純化目的。最後在elute 步驟中放入含有free glutathione 的elute buffer，就能使GST-protein 脫離resin 並溶解於elute buffer。

Glutathione Sepharose

Price: $49.00

Catalog#:
6555-1 | Size: 1 ml

Product Highlights:

• CONTENTS: Supplied as a 50 % slurry in 20 % Ethanol / PBS; > 3 mg (10
µmol) glutathione per ml Sepharose beads.

• FEATURES: The Glutathione-S-Transferase (GST) gene fusion system has been
widely used for the over expression of foreign genes in E. Coli. The expressed
fusion protein with a GST tail can be easily purified by affinity
chromatography on Glutathione-Sepharose beads from the bacterial lysate.
BioVison’s Glutathione-Sepharose is designed for the purpose of purification of
such GST fusion proteins or any other kinds of glutathione binding proteins.
This formulation exhibits excellent binding capacity, high flow rate, no
significant loss of the glutathione ligand and a pH stability range of 2-10.

• APPLICATIONS: Purification of GST-fusion proteins or other
glutathione-binding proteins.

Description: Glutathione-Sepharose is
prepared by covalently coupling glutathione to epoxy-activated 4 % cross-linked
sepharose beads to form a stable thioether linkage. The coupling was optimized
to give a high binding capacity and could be greater than 5 mg of
Glutathione-S-transferase (GST) per ml of wet gel.

Storage Temp.: +4°C

Shipping: gel pack

Usage: For Research Use Only! Not For Use in Humans.

其它GST標誌蛋白質純化-相關產品

Displaying 1 to 14 (of 14
products)

Result Pages: 1

Catalog#	Product Name	Size	Price+
K263-100	GST Colorimetric Activity Assay Kit	100 assays	$325.00
K260-100	GST Fluorometric Activity Assay Kit	100 assays	$325.00
1555R-1000	GST Inhibitor-1 (Cibacron Blue 3G-A, Sodium Salt)	1 g	$119.00
1556-1000	GST Inhibitor-2 (Ethacrynic acid))	1 g	$79.00
3997-100	GST-Tag Antibody	100 µg	$225.00
1243-1	Active GST Protein	1 mg	$109.00
7001-100	c-Jun (1-79)-GST Fusion Protein	100 µg	$145.00
6555-1	Glutathione Sepharose	1 ml	$49.00
6555-10	Glutathione Sepharose	10 ml	$225.00
6555-50	Glutathione Sepharose	50 ml	$775.00
7003-100	GSK-3a-GST Fusion Protein	100 µg	$125.00
4846-10	UbcH1, human recombinant (GST-tag)	10 μg	$125.00
4846-100	UbcH1, human recombinant (GST-tag)	100 μg	$335.00
4855-50	UCHL1, human recombinant (GST-tag)	50 μg	$275.00