Peroxidase Activity Assay Kit
Catalog#: K772-100 | Size: 100 assays




過氧化酶分析套組




同時可以使用呈色或螢光分析,
二種方式測量



呈色分析之靈敏度為0.1mU,
螢光分析靈敏度為0.01mU





Assa
y Kit Name:
Peroxidase Activity Assay Kit


Kit Summary:
• Detection method- Absorbance (570 nm) or Fluorescence (Ex/Em=535/587)
• Species reactivity- Mammalian
• Application- The kit has a detection limit of 0.1 mU per well via colorimetric or 0.01 mU per well via fluorometric method, based on our unit definition.


Features & Benefits:
• Simple procedure
• Fast and convenient
• The assay is simple, direct, highly sensitive and high-throughput adaptable


Kit Components:
• Assay Buffer
• OxiRed™ Probe (in DMSO)
• H2O2 Substrate (0.88 M)
• HRP Positive Control


Description:
Peroxidases (EC number 1.11.1.x) are a large family of enzymes that typically catalyze a reaction of the form: ROOR' + electron donor (2 e-) + 2H+ → ROH + R'OH. For many of these enzymes the optimal substrate is hydrogen peroxide, but others are more active with organic hydroperoxides such as lipid peroxides. Peroxidases can contain a heme cofactor in their active sites, or alternately redox-active cysteine or selenocysteine residues. BioVision’s Peroxidase Assay Kit provides a convenient colorimetric and fluorometric means to measure the peroxidase activity in biological samples. In the presence of Peroxidase, the OxiRed Probe reacts with H2O2 in a 1:1 stoichiometry to produce the red-fluorescent oxidation product, resorufin. The resorufin is quantified by colorimetric (lmax = 570nm) or fluorometric methods (Ex/Em = 535/587 nm). The assay is simple, direct, highly sensitive and high throughput-ready. The detection limit is 0.1 mU per well via colorimetric or 0.01 mU per well via fluorometric method, based on our unit definition.


Storage Conditions:
-20°C


Shipping Conditions:
gel pack


USAGE: For Research Use Only! Not For Use in Humans.


【供應廠商】太鼎生物科技有限公司



【連絡人】許虹宜 0920-312382



【台北公司】02-86609496



【相關網址】 www.biopioneer.com.tw



【原廠網址】http://www.biovision.com


 

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