太鼎生物科技/太鼎食安科技

組蛋白去乙醯酶3（Histone Deacetylase 3）ELISA酵素免疫分析套組

HDAC3 Activity Assay Kit
Catalog#: K343-100  | Size: 100 assay.

Kit Summary:
• Detection method- Fluorescence (Em/Ex = 380/500 nm) 
• Sample type- Cell and Tissue lysates, plasma and serum, other biological fluids 
• Species reactivity- Mammalian 
• Applications- Suitable for high throughput measurement of HDAC3 activity in purified, immunoprecipitated and recombinant or genetically modified HDAC-3 samples.

Features & Benefits: 
• Simple procedure; takes ~ 60 min 
• Fast and convenient 
• Kit contains the necessary reagents for accurate measurement of HDAC3 activity

Kit Components: 
• HDAC3 Assay Buffer 
• HDAC3 Substrate 
• HDAC3 Positive Control 
• AFC Standard (1 mM) 
• Developer 
• Trichostatin A (10 µM)

Description: 
Histone deacetylases (HDACs) represent a large family of enzymes identified as key regulators of nucleosomal histone acetylation, a major event that controls eukaryotic gene transcription and are classified into three groups. HDACs are transcriptional repressors with crucial roles in mammalian development. They are believed to be involved in important biological activities, such as cell differentiation, proliferation, apoptosis, and senescence. In BioVision’s HDAC-3 activity Kit, HDAC-3 and Developer will deacetylate and cleave the substrate [R-H-K-K(Ac)-AFC] to release the quenched fluorescent group (AFC), which can be detected at Em/Ex = 380/500 nm. The kit provides a rapid, simple, sensitive, and reliable test, which is also suitable for high throughput measurement of HDAC-3 activity in purified, immunoprecipitated and recombinant or genetically modified HDAC3 samples.

Storage Conditions: 
-20°C (Upon receipt place HDAC-3 positive control at -80 °C for storage.)

Shipping Conditions: 
gel pack