BacAway, G285
廣泛型抗生素添加劑，有效預防革蘭氏陰性菌及革蘭氏陰
性菌之污染。
Cat.No.: | G285 |
Quantity: | 2 x 1.25ml |
Description | Bacterial contamination is often associated with long-term culture of primary cells, stable cell lines, and stem cells. It is often frustrating that cells got contaminated after weeks or months of clone selection or precious establishment of primary cells. Most antibiotics like Ampicillin and others found in most labs can not solve the problems, leading to loss of precious cells. To this end, scientists at ABM have successfully developed a new formulation of antibiotics that can kill any bacteria associated with cell culture. The reagent is so effective that you will once-for-all worry-free when culturing precious cells. |
Storage | This product is stored at -20°C , and is stable for 60 months if properly stored. Avoid freeze-thaw cycles by making aliquots upon first thawing this product. |
Data Sheet |
Protocol
Prepare 1. treatment medium by adding BacAway to sterile cell culture medium in a 1:250 dilution.
Wash contaminated cells three times using sterile medium.2.
Add3. treatment medium to the cell culture. Replace with new treament medium after 3 hours and 7 hours.
After incubating the cell culture overnight, visually examine the medium. If 4. bacteria are still visible under the microscope, continue to 5. If not, continue to 7.
Trypsinize and centrifuge the cells. Carefully discard the supernatant, then 5. re-seed the cells in a new culture vessel containing treatment medium.
Incubate the cells for 6-7 hours, then replace the medium with new 6. treatment medium.
After incubating overnight, replace the medium with new 7. treatment medium once per day for one week. This will completely eliminate any remaining bacteria.
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