太鼎生物科技/太鼎食安科技

 太鼎生物科技有限公司(02)86609496

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   As a world leader in lentiviral technology, ABM has developed a variety of different lentiviral expression systems which can be used to temporarily alter expression within a sample of cells or to moniter gene expression using reporter genes. These systems allow other scientists to produce their own lentiviruses.

Lentiviral 表現系統產品種類

Lentiviral Systems

Lentivirus Constitutive Vectors表現載體

Lentivirus CMV啟動子載體 

His Tag Lentiviral 載體 | HA Tag Lentiviral 載體 | HA-III Tag Lentiviral 載體

Lentivirus UbC, PGK or EF1α啟動子載體 

UbC Promoter Lentiviral載體 | PGK Promoter Lentiviral 載體 | EF1α Promoter Lentiviral 載體

Lentivirus 啟動子缺失載體

 Promoterless Lentiviral 載體 | Promoterless GFP Lentiviral 載體

Bicistronic/Tricistronic Lentiviral 載體

shRNA and miRNA Lentiviral 表現載體

RFP Fusion Tag Lentiviral 載體

綠螢光蛋白GFP Fusion Tag Lentiviral 載體

綠螢光蛋白GFP Bicistronic Lentiviral載體

Reporter Lentiviral 載體

Therapeutic Lentiviral 載體

Lentivirus 包裝系統

   As a world leader in lentiviral technology, ABM has developed two unique packaging mixtures. Each contains two or three plasmids to seperate the lentiviral structural and replication genes until time of viral production, and preventing the structural genes from being present in the packaged viral genome of the produced virus. This increases the safety for the user by making the produced viruses replication incompetent.

Products List

Lentiviral 包裝系統

產品介紹

  For the production of lentiviral particles, three components are generally required: 1) a lentiviral vector containing your inserts of interests (cDNA, shRNA, or miRNA), 2) one or two packaging vectors which contain all necessary viral structure proteins, 3) an envelope vector expressing Vesicular Stomatitis Virus (VSV) glycoprotein (G). The 3^rd(third) generation packaging system offers maximal biosafety as the lentiviral Rev gene is supplied as an independent vector from other structure genes, further eliminating the possibility of reverse recombinantion of vectors into a replication competent viral particles. The 3^rd(third) generation lentiviral packaging mix will only support lentiviral expression vector with a chimeric 5' LTR in which the HIV promoter is replaced with CMV or RSV, thus making it TAT-independent. Thus the 3^rd(third) generation lentiviral vectors will not support the production of 2^nd(second) generation lentiviral particle productions. All of the lentiviral vectors marketed by ABM are TAT-independent.

2^nd Generation Packaging Mix

3^rd Generation Packaging Mix

Lentivirus純化及定量套組

Lentivirus 純化 Kit

PuRetro™ Lentivirus Purification Kits產品特點

easy to use

very reliable

very affordable

Speedy Lentivirus 純化

Speedy Lentivirus 純化產品特點

  Recombinant lentiviral vectors have been shown to be a powerful tool for stable gene transfer to both dividing and non-dividing cells in vitro and in vivo.Through years of experience with lentiviral vectors, ABM has developed its own proprietary pLenti-combo packaging mix and an efficient protocol for rapid production of recombinant lentiviral vectors with titers up to 107IU/ml. The quickest and easiest way to purify and concentrate lentiviral particles up to 100x. The concentrated viral preparations can be used for in vivo injection or like applications that require high titer of viral particles.

ABM's Lentiviral表現載體產品特點

stable plasmid; rare plasmid DNA re-arrangement

very high yielding plasmid

no special competent cells required

His and HA tag compatible

ABM is THE source for your lentiviral expression needs!

Viral Stock Buffer Exchange Kit

Viral Stock Buffer Exchange Kit (Cat.# G130) is supplied separately from the recombinant viral purification kits. All recombinant viral purification kits do not contain the viral stock buffer exchange kit.
  The eluted recombinant virus is in a salt buffer but can be used directly for in vitro target cell transduction if the viral dilution is over 5X (i.e. 1.0μl of viral stock to 4μl culture medium). For higher titers of viral stock or viral dilutions less than 5X during transduction, a buffer exchange is required to make the viral stock suitable for such applications. This can be easily performed using ABM Inc.'s viral buffer exchange kit (Cat.# G130). The kit includes five exchange filters that are applicable for all recombinant viruses, as well as two specialized buffers, in sufficient quantities to run either five adenoviral or five lenti/retroviral or any combination of purifications.
Adenoviral storage buffer: 2.5% glycerol (w/v), 25mM NaCl, and 10mM Tris-HCl, pH 8.0 (Hoganson, et al., 2002).
Lentiviral/Retroviral storage buffer: 1X PBS buffer, pH at 7.2.

qPCR Lentivirus Titer (Titration) Kit

The Lentivirus Titration Kit provides a fast and simple method for titrating lentivirus. This kit employs a quick RNA extraction step and determines viral RNA using qRT-PCR. The whole assay could be completed in only 2 hours. The titer primers supplied in this kit are designed for all HIV-1 based vectors detection. With the help of the on-line titer calculator, titration of any lentiviral preparation could be easier than ever before.

ABM's Lentiviral qPCR Titer Kit病毒力價定量產品特點

no RNA purification -- saves time and eliminates inaccuracy

ready-to-use reagent mix -- reduces variability

qRT-PCR in one step -- more sensitive and accurate than other methods

no NTC amplification -- our titeration kit is the only one on the market that completely elimites NTC signal due to our unique primer design. Similar kits from other companies all give NTC amplification, which compromise accuracy for viral titeration

free qPCR mastermix -- a deal that can not be beat

詳細產品資訊，請登錄www.abmgood.com 查詢，或與太鼎生物科技有限公司(02)86609496聯繫。 

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太鼎生物科技有限公司(02)86609496