【如何快速有效準備好您的genomic DNA】DirectPCR Lysis Reagents一個步驟反應，即可將DNA由組織中釋放，並且不須經由任何純化的步驟，進行genotyping分析。 @ 太鼎生物科技/太鼎食安科技

基因型鑑定知多少?

【如何快速有效準備好您的genomic DNA】

組織均質後, 某些複合物會影響後續PCR反應，專利研發DirectPCR Lysis Reagents具有抑制PCR抑制物的反應，因此，一個步驟反應，即可將DNA由組織中釋放，並且不須經由任何純化的步驟(例如Phenol/Chloroform萃取, 或管柱純化)，溶液可以直接PCR，進行genotyping分析。如此方便快速的方法, 可以應用於尾巴genomic DNA萃取、細胞genomic DNA萃取、耳朵genomic DNA萃取、卵黃囊genomic DNA萃取。

最快速及操作簡單的genotyping kit,

1. 只須一個步驟，無須再經DNA純化步驟，效率100%。

一個步驟反應，即可將DNA由組織中釋放，並且不須經由任何純化的步驟

2. 專利研發專為genotyping分析而設計。

「基因型鑑定」是全面搜尋人類致病基因的重要分析技術。Genotyping基因型鑑定，快速有效萃取動物組織, 尾巴基因型DNA (genomic DNA)，不須經由Phenol/Chloroform萃取步驟, 不須再通過colum等繁瑣步驟純化，只要一個步驟，效率100%。

DirectPCR DNA Extraction Reagents
Genotyping without DNA isolation

Description: Viagen DirectPCR® DNA Extraction System is a single-tube system for rapid preparation of DNA from mouse tails, ear pieces, yolk sac, and culture cells. The patent-pending components developed by scientists at Viagen Biotech Inc. allow that the resulting DNA extracts are compatible with genomic PCR for genotyping. (more)

Simplified procedure:
1. Lyse tails in DirectPCR reagents.
2. Incubate for 45 min at 85°C.
3. PCR using 1 µl lysates.

Detailed protocols: Tail, Ear, Yolk Sac, Cultured cells.

Representative PCR results

DirectPCR Products: Tail, Ear, Yolk Sac, Cultured cells.

New !! Proteinase K Solution : For a small number of tails, genomic PCR quality..

Order / Prices

DirectPCR DNA Extraction Reagents	Cat#	Comments	Price
DirectPCR Lysis Reagent (mouse tail)	[101-T]	250 mouse tails (50 ml)	$3950	Order
DirectPCR Lysis Reagent (mouse tail)	[102-T]	500 mouse tails (100 ml)	$6950	Order
DirectPCR Lysis Reagent (yolk sac)	[201-Y]	yolk sacs (50 ml)	$3950	Order
DirectPCR Lysis Reagent (yolk sac)	[202-Y]	yolk sacs (100 ml)	$6950	Order
DirectPCR Lysis Reagent (cell)	[301-C]	cultured cells (50 ml)	$3950	Order
DirectPCR Lysis Reagent (cell)	[302-C]	cultured cells (100 ml)	$6950	Order
DirectPCR Lysis Reagent (mouse ear)	[401-E]	mouse ear (25 ml)	$3950	Order
DirectPCR Lysis Reagent (mouse ear)	[402-E]	mouse ear (50 ml)	$6950	Order
Proteinase K Solution	[501-PK]	US and Canada only	$4950	Order