太鼎生物科技/太鼎食安科技

螢光細胞增生分析套組, BIOVISION K303-500

VisionBlue™ Quick Cell Viability Assay
Catalog#: K303-500  | Size: 500 assays

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螢光細胞增生分析套組, BIOVISION K303-500

VisionBlue™ Quick Cell Viability Assay
Catalog#: K303-500  | Size: 500 assays

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安全性佳, 除了測量細胞增殖分析外, 可以再進行下游分析

Very safe: Non-radioactive, non-toxic, and cells can be further used for other experiments..

Kit Summary:
• Detection method- Fluorescence (Ex/Em 530–570/ 590-620 nm)
• Sample type- Cell culture (adherent and suspension)
• Species reactivity- Mammalian
• Kit size- Convenient sizes (500, 2500)
• Applications- This fluorescence-based single-step cell viabilty assay utilizes the redox dye (Resazurin) which is not fluorescent, but upon reduction by metabolically active cells, it becomes highly fluorescent (Ex = 530-570 nm; Em = 590 -620 nm). Therefore, viable metabolically active cells can be easily measured.

Features & Benefits:
• Simple one-step procedure
• Fast and convenient
• The assay is more sensitive than other similar assays (eg, Almar Blue reagent) on the market

Kit components:
• VisionBlue™ Reagent

Description:
The VisionBlue™ Quick Cell Proliferation Assay Kit provides by far the most sensitive and easiest means for quantifying cell proliferation and viability. Simply add the single reagent to cell culture, incubate and read the fluorescence intensity. The VisionBlue™assay utilizes the redox dye resazurin which is not fluorescent, but upon reduction by viable metabolically active cells, the dye becomes highly fluorescent (Ex = 530-570 nm; Em = 590-620 nm). Therefore, viable metabolically active cells can be easily measured.

Storage Conditions:
-20°C

Shipping Conditions:
gel pack

USAGE: For Research Use Only! Not For Use in Humans

The Quick Cell Proliferation Assay Kit provides all reagents and detailed instructions for a fast and sensitive quantification of cell proliferation and viability. The assay is based on the cleavage of the tetrazolium salt WST-1 to formazan by cellular mitochondrial dehydrogenases. Expansion in the number of viable cells resulted in an increase in the activity of the mitochondrial dehydrogenases, which leads to the increase in the amount of formazan dye formed. The formazan dye produced by viable cells can be quantified by multi-well spectrophotometer (microtiter plate reader) by measuring the absorbance of the dye solution at 440 nm. The assay can be used for the measurement of cell proliferation in response to growth factors, cytokines, mitogens, and nutrients, etc. It can also be used for the analysis of cytotoxic compounds like anticancer drugs and many other toxic agents and pharmaceutical compounds. The new method is so simple, requiring no washing, no harvesting, and no solubilization steps, and is faster and more sensitive than MTT, XTT, or MTSbased assays. The entire assay can be performed in a microtiter plate.