快速CRISPR/Cas9檢測試劑盒

 

品牌ABMGOOD 貨號: G 規格: 25個反應

CRISPR Genomic Cleavage Detection Kit提供快速突變篩選方法。

G932%20image.png

 

CRISPR Genomic cleavage detection產品規格

CRISPR Genomic Cleavage Detection Kit, G932

Cell Lysis Buffer 1.25 ml G932-1

Protein Degrader 50 μl G932-2

Detection Enzyme 13 μl G932-3

10X Detection Buffer 50 μl G932-4

Control Primer & Template 10 μl G932-5

2X PCR Bestaq™ MasterMix 1 ml G464

Nuclease-free H2O 1 ml RT-0


CRISPR-edited samples are used as a template in PCR reactions targeting your specific region of interest. The products are then denatured and re-annealed to produce mismatches within the double strand. Our detection enzyme is able to recognize such mismatches and cleaves the strands to produce band sizes that are easily distinguishable upon gel analysis. The ready-to-use CRISPR Genomic Cleavage Detection Kit conveniently contains all the necessary reagents required, including a set of control template and primers to ensure reliable results. With a short processing time, this ready-to-use assay will be a great addition to any genomic-editing

 

Features:
• Ease of use with simple steps
• Rapid set-up
• Streamlined protocol suitable for high-throughput applications

 

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CRISPR文獻引用

 

Chung, G et al. "CHL-1 provides an essential function affecting cell proliferation and chromosome stability in Caenorhabditis elegans." DNA Repair (Amst.) 10 (11):1174- 1182 (2011). DOI: 10.1016/j.dnarep.2011.09.011. PubMed: 21968058. Application: PCR.

 

Bhagwat, B et al. "An in vivo transient expression system can be applied for rapid and effective selection of artificial microRNA constructs for plant stable genetic transformation." J Genet Genomics 40 (5):261-270 (2013). DOI: 10.1016/j.jgg.2013.03.012. PubMed: 23706301. Application: PCR.

 

Jones, MR et al. "The atm-1 gene is required for genome stability in Caenorhabditis elegans." Mol. Genet. Genomics 287 (4):325-335 (2012). DOI: 10.1007/s00438-012-0681-0. PubMed: 22350747. Application: PCR.

 

Chung, G et al. "CHL-1 provides an essential function affecting cell proliferation and chromosome stability in Caenorhabditis elegans." DNA Repair (Amst.) 10 (11):1174-1182 (2011). DOI: 10.1016/j.dnarep.2011.09.011. PubMed: 21968058. Application: PCR.

 

Lee, G et al. "Inhibition of in vitro tumor cell growth by RP215 monoclonal antibody and antibodies raised against its anti-idiotype antibodies." Cancer Immunol. Immunother. 59 (9):1347-1356 (2010). DOI: 10.1007/s00262-010-0864-7. PubMed: 20473495. Application: PCR.

 

Chaiyakul, M et al. "Cytotoxicity of ORF3 proteins from a nonpathogenic and a pathogenic porcine circovirus." J. Virol. 84 (21):11440-11447 (2010). DOI: 10.1128/JVI.01030-10. PubMed: 20810737. Application: PCR.

 

Lee, G et al. "Inhibition of in vitro tumor cell growth by RP215 monoclonal antibody and antibodies raised against its anti-idiotype antibodies." Cancer Immunol. Immunother. 59 (9):1347-1356 (2010). DOI: 10.1007/s00262-010-0864-7. PubMed: 20473495. Application: PCR.

 

Ahn, CH et al. "Bacterial biofilm-community selection during autohydrogenotrophic reduction of nitrate and perchlorate in ion-exchange brine." Appl. Microbiol. Biotechnol. 81 (6):1169-1177 (2009). DOI: 10.1007/s00253-008-1797-3. PubMed: 19066883. Application: PCR.

 

Lee, G et al. "Cancer cell expressions of immunoglobulin heavy chains with unique carbohydrate-associated biomarker." Cancer Biomark 5 (4):177-188 (2009). DOI: 10.3233/CBM-2009-0102. PubMed: 19729827. Application: PCR. 

 

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