太鼎生物科技/太鼎食安科技

Service

Description

Cat. No.

Adenovirus Purification

Amplify and purify (2-3 ml of 10⁹ pfu/ml)

C070

Amplify and purify (2-3 ml of 10¹⁰ pfu/ml)

C071

Amplify and purify (5 x 200 μl of 10¹¹ pfu/ml)

C072

Amplify and purify (5 x 200 μl of 10¹² pfu/ml)

C005

Vector

pAdenoG-IRES-GFP

Vector Size

37302bp

Promoter

CMV

Insert

TBX18

Insert Size

1824 bp

Organism

Human

Accession #

BC132715

Chromosome

6Q14.1-Q15

Tags

GFP reporter (IRES)

Gene Type

Wild Type. Mutants can be constructed via custom services. Please refer to our custom service page or contact our technical support for more info.

Titer

1x10⁶pfu/mL

Storage Buffer

DMEM with 2.5% glycerol

Shipping & Storage

Adenoviruses are stable at room temperature for short term storage and are shipped with gel ice packs. Do not store at -20°C or 4°C for long term. For long term storage, it is recommended to store the viruses at -80°C and avoid repeated freeze-thaw cycles. Before storing at -80°C, it is advisable to make small aliquots to avoid repeated freeze-thaw cycles. Screw cap vials are recommended to decrease chances of the storage buffer desiccating which leads to decrease in infectivity. Diluting the virus is not recommended.

Disclaimer

1. Disclaimer for transcript variants: The provided accession number refers to the transcript (mRNA) sequence for this product. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. All sales are final.
2. The provided accession number refers to the transcript (mRNA) sequence for this product. Please verify that this is the desired transcript sequence by cross-referencing. This is important because a single gene can have multiple different transcripts owing to naturally occurring variations. All sales are final.
3. Disclaimer for intended use: All of abm's vectors and viral particles are for research use ONLY and NOT for therapeutic/diagnostic applications. abm is not liable for any repercussions arising from the use of its vector(s) in therapeutic/diagnostic application(s).
4. Disclaimer for extra nucleotides: Cloning may lead to the insertion of extra nucleotides at the 5' or 3' end of the gene of interest which, in most cases, is innocuous to the stability/functionality and the expression of that gene.

Guarantee

abm guarantees that the correct ORF construct is provided and the mRNA expression is displayed upon successful transduction. If this is not the case, we will provide a one-time replacement. Customers must provide adequate data to show >80% transfection efficiency with a positive control, plus additional qPCR data or a western blot to evaluate the level of gene expression. The replacement will not be covered by the same guarantee.

Please note that due to the large number of variables applicable, any further expression analysis (e.g. protein expression) is not covered by the guarantee, as such analysis is dependent on the end user's experimental conditions.