Growth factors, cytokines, chemokines and hormones share many similarities and act as essential biochemical messengers for housekeeping functions, as well as, having crucial roles in pathological and stem-cell related growth and regulation.
Each performs both homeostatic and stimulatory functions. Moreover, typical implications of a positive effect on cell division associated with growth factors are also evident with some cytokine and hormonal stimulatory signals. Indeed, some cytokines and hormones are growth factors and the distinction between them is frequently arbitrary and stems more from the manner of their discovery rather than from a clear difference in function. In addition, some immunomodulatory effects of cytokines are systemic rather than only local. In hormonal terms, this means cytokines may be autocrine or paracrine in chemotaxis and endocrine as a pyrogen.Chemokines, a subclass of cytokines, also show pro-inflammatory and homeostatic activities. BioVision offers a huge repertoire of these essential biomolecules. Our product line comprises over 300 items (with the exception of two, all are recombinant). In addition, we offer a huge complementary line of antibodies to recognize many of these proteins.
Anti-15 K allergen of house dust mite Dermatophagoides farinae, excretion (DfA) Antibody
Antibody Type
Primary Antibody
Description
Anti-15 K allergen of house dust mite Dermatophagoides farinae, excretion (DfA) is a This product is sold as an antibody preparation for research use only. Standard Laboratory Practices should be followed when handling this material. Contains sodium azide (0.1 %) as preservative. Although the amount of sodium azide is very small appropriate care must be taken when handling this product.
Immunogen
Purified DfA
Specificity
DfA
Tested Applications
ELISA and WB
Application Notes
Detection of DfA by ELISA and Western blotting.
Raised In
Df10 Hybridoma clone has been derived from hybridization of Sp2/0 myeloma cells with spleen cells of Balb/c mice immunized with purified DfA.
Clonality
Monoclonal
Isotype
IgG1 for MAb Df10
Purity
Chromatography on protein G Sepharose
Storage Buffer
PBS, pH 7.4 with 0.05% sodium azide.
Formulation
Liquid
Concentration
1ug/ul
Storage
+ 4 °C
Caution
Anti-15 K allergen of house dust mite Dermatophagoides farinae, excretion (DfA) is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information.
Bischoff JR, Anderson L, Zhu Y, Mossie K, Ng L, Souza B, Schryver B, Flanagan P, Clairvoyant F, Ginther C, Chan CS, Novotny M, Slamon DJ, Plowman GD. A homologue of Drosophila aurora kinase is oncogenic and amplified in human colorectal cancers. EMBO J. 1998, 17(11):3052-65.
Marumoto T, Zhang D, Saya H. Aurora-A - a guardian of poles. Nat Rev Cancer. 2005, 5(1):42-50.
Lin SY, Makino K, Xia W, Matin A, Wen Y, Kwong KY, Bourguignon L, Hung MC. Nuclear localization of EGF receptor and its potential new role as a transcription factor. Nat Cell Biol. 2001, (9):802-8.
上述這些訊號傳遞作用和細胞週期之進行具有什麼相關呢？基本上細胞週期是由三件事調控，即生長刺激因子(mitogens 例如growth factors，cytokines )、與其他細胞之接觸(contact with other cells )、固著於基質層(anchorage to a substratum )。細胞週期之進行需要生長刺激因子，即這類訊號會使細胞進入細胞週期。然而細胞週期是受接觸抑制的(contact inhibtion)，所以當空間太小時，細胞便不會進入細胞週期。此外細胞也必須固著至某個基質層(例如透過ECM)，而integrin之重要性即在於此---它們調控了細胞週期之固著依存性（anchorage-dependence）。由上所述我們知道 mitogens 和integrins會一起協同調控細胞週期之進行，單獨一項是無法成功達成的。如果我們同時將細胞置於ECM上並給予生長因子（一種mitogens），細胞週期便會啟動。然而這對癌細胞而言又如何呢？癌細胞生長常為不依存生長刺激因子及基質層固著。
Reference:
Vuori, K. (1998). Journal of Membrane Biology 165: 191-199.
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Commercially available antibodies often contain substances (e.g. BSA, glycine, tris, azide) that interfere in labeling reactions. The AbSelect™ Purification Kit quickly removes these contaminants. It can also be used to purify antibodies from crude samples such as ascites fluid or immune serum.
The antibody to be purified or cleaned up ideally is in a volume of 100ul to 0.5ml. Up to 500µg of antibody can be purified in each run. The AbSelect method involves capture of the antibody on protein A resin and the removal of unwanted substances by a simple wash procedure. The purified product is then eluted and neutralized.
The components of the AbSelect kit are fully compatible with the Lightning-Link™ antibody labeling system (available separately), which allows the purified antibody to be rapidly conjugated with a wide variety of labels.
Antibodies generated from ascites fluid and serum are often supplied as crude formulations. Both ascites fluid and serum contain many substances that interfere with antibody labeling reactions. Successful labeling therefore requires antibodies to be purified prior to labeling. The AbSelect Serum system is a fast and simple method to purify antibodies from these types of media.
AbSelect Serum is prepared by coupling highly purified protein A to agarose beads which can be used to purify IgG fractions from ascites fluid and serum. The method involves the capture of the antibody on the AbSelect Serum resin and the removal of unwanted substances using a simple wash procedure. The purified product is then eluted and neutralized.
The components of the AbSelect Serum purification system are fully compatible with the Lightning-Link™ antibody labeling kits (available separately), which allows the purified antibody to be immediately labeled with an enzyme, fluorescent protein or fluorescent dye, with a hands-on time of under 30 seconds.
Antibodies are often generated from hybridoma cell lines and supplied in tissue culture supernatant (TCS). TCS itself contains many substances that interfere with antibody labeling reactions. Successful labeling therefore requires antibodies to be purified prior to labeling. The AbSelect TCS system is a fast and simple method to purify these antibodies.
AbSelect TCS is prepared by coupling highly purified protein A to agarose beads which can be used to purify IgG fractions from hybridoma supernatants. The method involves the capture of the antibody on the AbSelect TCS resin and the removal of unwanted substances using a simple wash procedure. The purified product is then eluted and neutralized.
The components of the AbSelect TCS are fully compatible with the Lightning-Link™ antibody labeling kits (available separately), which allows the purified antibody to be immediately labeled with an enzyme, fluorescent protein or fluorescent dye, with a hands-on time of under 30 seconds.
Antibodies are sometimes only available at low concentrations and often contain low molecular weight substances that interfere in labeling reactions with enzymes, biotin, streptavidin and fluorophores. The Antibody Concentration and Clean Up Kit allows for the quick and easy concentration of antibodies and proteins. The kit can also be used to reduce the concentration of many unwanted additives often found in antibody formulations such as azide, glycine or tris.
The antibody clean up kit method utilizes a simple spin column to easily and quickly remove excess buffer from the antibody thereby providing a more concentrated antibody solution. The clean up kit also allows the experimenter to perform a simple buffer exchange to transfer the antibody into a more favorable conjugation buffer.
★Signalway Antibody (SAB) will be attending or supporting the American Association for Cancer Research - AACR,Neuroscience 2011,American Society for Cell Biology-ASCB.Please feel free to come to our booth and meet with us.Both Technical and Sales personnel will be attending the meetings listed and will be happy to answer any questions you may have.
2011 Promotion!
Free antibody you can get Now purchasing 3 antibodies in a single order,you will get the cheapest one in the order free! Please pay attention that the promotion will expire on June 30,2011.
2 free antibodies you can get Publishing papers using Signalway Antibody primary antibodies,you will have a chance to get 2 free antibodies (50ul) from SAB.More details about the promotion,please kindly click the link!
Hybridoma cell linesAntibodies are often generated from hybridoma cell lines and supplied in tissue culture supernatant (TCS). TCS itself contains many substances that interfere with antibody labeling reactions. Successful labeling therefore requires antibodies to be purified prior to labeling. The AbSelect TCS system is a fast and simple method to purify these antibodies.
AbSelect TCS is prepared by coupling highly purified protein A to agarose beads which can be used to purify IgG fractions from hybridoma supernatants. The method involves the capture of the antibody on the AbSelect TCS resin and the removal of unwanted substances using a simple wash procedure. The purified product is then eluted and neutralized.
The components of the AbSelect TCS are fully compatible with the Lightning-Link™ antibody labeling kits (available separately), which allows the purified antibody to be immediately labeled with an enzyme, fluorescent protein or fluorescent dye, with a hands-on time of under 30 seconds.
Hands on time to set up conjugation reaction is 20-30 seconds.
Supplied dialysis cartridge ensures conjugates are recovered in high yield.
BSA, Ovalbumin or Keyhole Limpet Hemocyanin (KLH) stimulate a large immune response and are typically used as carrier proteins to generate antibodies against small antigens (peptides or organic compounds). For success the antigen (normally referred to as a hapten) needs to be covalently linked to the carrier protein.
The imm-Link™ sulfhydryl conjugation kit allows sulfhydryl containing haptens to be conjugated to a carrier protein simply by adding a solution of the hapten to a proprietary lyophilised mixture containing the carrier protein and all the required conjugation chemistry. The hands-on time to set up the conjugation reaction is typically 20-30 seconds.
Following successful conjugation the conjugate is dialysed in the supplied dialysis cartridge to remove unwanted by products. The hapten conjugate can then be easily recovered and used.
INNOVA BIOSCIENCES集力開發免疫標誌產品 Innova Biosciences (Cambridge, UK) the inventor of the World's easiest to use antibody labeling kits, has extended its product portfolio.
CAMBRIDGE, ENGLAND, February 24, 2011 /Science and Research PR News/ -- Innova Biosciences (Cambridge, UK) the inventor of the World's easiest to use antibody labeling kits, has extended its product portfolio. For scientists to generate novel antibodies, it is necessary to perform a conjugation reaction (the joining of two molecules) to link the target protein or peptide to a carrier protein such as BSA or Ovalbumin. Conjugation reactions are traditionally daunting and laborious to the non-specialist.
Innova Biosciences imm-Link kits have been designed to take the difficulty out of this conjugation process through simplifying both the linking chemistry and the methodology itself - the conjugate is created simply by adding the target protein or peptide to a freeze dried mixture containing the relevant chemistry and the carrier protein.
BACKGROUND: Green fluorescent protein (GFP) is a spontaneously fluorescent protein isolated from pacific jellyfish, Aequorea victoria. It transduces the blue chemiluminescence into green fluorescent light. Since the molecular cloning of GFP cDNA and demonstration of GFP as a functional transgene, GFP has become a powerful tool with exciting applications in developmental, cell and molecular biology. GFP fluorescence is not species specific and can be expressed in bacteria, yeast, plant and mammalian cells. GFP can fuse with proteins of interest without interfering significantly with their assembly and function.
SPECIFICITY: The antibody reacts with GFP, and its variants EGFP, RFP, YFP, and CFP, etc. Recombinant EGFP (Cat.# 4999-100), RFP (Cat.# 4997-100), YFP (Cat.# 4998-100) and CFP (Cat.# 4996-100) can be used as positive controls.
APPLICATION AND USAGE: The antibody can be used for Western blotting (0.5-4 ug/ml). Based on researcher's feedback, it can also be used in immunoprecipitation (10-20 ug/ml). However, the optimal conditions should be determined individually.
STORAGE CONDITIONS: Store at -20oC. For long-term storage, aliquot and freeze at -70oC. Avoid repeated freeze/defrost cycles.
(sufficient for 8 purifications with 200 ml of rabbit cell culture supernatant and/or ascites fluid/each)
Rabbit IgG Binding Gel (SepharoseTM fast flow) (Code : RbIKG-FF) : 5 ml gel column. Binding capacity : approx. 10 mg Rabbit IgG/ml wet gel. Purity : 90% by SDS-PAGE. Maximum pressure : 3 bars (43 psi, 0.3 MPa). Gel life : approx. 50 cycles with routine regeneration.
Rabbit IgG Binding Buffer (Code : BBRbG) 2x concentrated: 1,000 ml (add 1000 ml of distilled water before use).
Rabbit IgG Elution Buffer (Code : EBRbG) 4x concentrated: 125 ml (add 375 ml of distilled water before use).
Rabbit IgG Precipitating Agent (Code : PARbG) : 8 x 1 sachet of sufficient quantity for precipitating all IgG from 200 ml of rabbit cell culture supernatant and/or ascites fluid.
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