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UVssDNA單股DNA--單株抗體 (C3B6)


UVssDNA單株抗體(C3B6)







Trevigen
is pleased to provide highly qualified enzymes, antibodies, and assay kits for
DNA damage and repair research. As always, our products are accompanied by
detailed product data sheets or instructions for use, and backed with expert
technical support.





DNA Repair
Deficient Cell Lines




DNA repair
pathways maintain the integrity of the genome reducing the onset of cancer,
disease and aging phenotypes. To further study repair pathways, Trevigen offers
panels of human cell lines each deficient in proteins associated with DNA
repair. This consists of cell lines deficient for proteins in the Base
Excision Repair Pathway
, Non
Homologous End Joining,
Homologous
Recombination
and Mismatch
Repair
.
For the availability of other DNA repair deficient
cell lines contact Trevigen.





PARP/PAR/PARG




PARP-1
contributes to the sequence of events that occurs during DNA base excision
repair. Trevigen offers kits that measure the in vivo and in vitro activities
of PARP and PARG.





CometAssay®




The single
cell gel electrophoresis or CometAssay®
is a state-of-the-art technique for quantitating DNA damage and repair from in
vivo and in vitro samples of eukaryotic cells and some prokaryotic cells. In
conjunction with Trevigen’s new CometAssay® Electrophoresis System, which
eliminates known causes of assay variability, it is the only technique that
directly measures DNA damage in individual cells and as a result has rapidly
gained importance in the fields of genetic toxicology and human biomonitoring.







DNA Repair Enzymes
and Antibodies




Trevigen’s
unique FLARE™ Assays characterize DNA damage in single cells using a variety of
DNA repair enzymes that recognize oxidative and UV damage in conjunction with
Trevigen’s CometAssay® Electrophoresis System.
A
variety of DNA repair enzymes and antibodies are available as individual
components with optimized assay conditions and buffers for research in areas of
double-strand break repair, base excision repair, DNA methylation, oxidative
damage, UV damage, and radiation damage.





 








































Anti-UVssDNA Monoclonal Antibody (clone
C3B6)




中文名稱




UVssDNA單株抗體(C3B6)




供應商




Trevigen




產品貨號




4350-MC-100




產品資料




應用於UV損傷皮膚之研究、UV損傷DNA之修復機理。




免疫原




UV輻射的小牛胸腺偶聯甲基化BSA之單鏈DNA (UV輻射的dT-mBSA聚合物)




來源宿主




抗體subtypeIgG1/κ。抗體經由小鼠腹水免疫後,再純化。抗體力價高。抗體濃度含有1mg/mlPBS 0.01%疊氮鈉。




反應性




識別單鏈DNA4個核苷酸長度的(6-4)-dipyrimidines。能特異識別(6-4)-dithymidine,不能識別(6-4)-dicytidines or cyclo-butadipyrimidines,與寡核苷酸序列裡的(6-4)-thymine/cytidines有較低的交叉反應。


Immunological assays for DNA photoproducts provide a rapid means to quantitate photodamage in DNA. Photochemical damage is considered to be an important factor in the development of skin cancer. Sensitive immunological methods that detect individual DNA adducts facilitate the study of the biological significance of DNA lesions. Trevigen’s monoclonal UVssDNA antibody (clone C3B6) (cat# 4350-MC-100) detects (6-4)-dipyrimidine photoproducts. Specificity has been demonstrated for (6-4)-dithymidine, whereas the (6-4)-dicytidines or cyclobutadipyrimidines are not recognized. A low level of cross reactivity was observed with repeating (6-4)-thymine/cytidines within an oligonucleotide sequence. The UVssDNA clone C3B6 antibody recognizes (6-4)-dipyrimidines in single stranded DNA at least 4 nucleotides long. 




保存建議




保存於-20貯存,避免反復冷凍解凍。








【供應廠商】太鼎生物科技有限公司



【連絡人】許虹宜0920-312382



【連絡信箱】taiding.biotech@msa.hinet.net



【台北公司】02-86609496



【公司網址】www.biopioneer.com.tw





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