【抗體標誌/抗體濃縮及純化套組】適用於腹水及血清產製抗體純化、融合瘤組織培養液(TCS)產製之抗體純化、低濃度抗體之純化及濃縮 @ 太鼎生物科技/太鼎食安科技

抗體純化套組---抗體濃縮及純化

AbSelect Antibody Purification System (860-0010)

Innova Bioseiences提供一系統抗體純化套組，每組套組包括抗體純化之buffer及resin，有效純化不同來源之抗體含量。其純化來源，可為市售抗體之純化(貨號#860-0010/rabbit; 貨號#830-0010/mouse; 貨號#890-0010/rat/sheep/human/goat)、腹水及血清產製抗體之純化(貨號#863-0500/rabbit; 貨號#893-0500/rat/sheep/human/goat/mouse)、融合瘤組織培養液(TCS)產製之抗體(貨號#862-0500/rabbit/human; 貨號#832-0500/mouse)、低濃度抗體之純化及濃縮(貨號#861-0010)。有效移除溶液中BSA、Glycine、Tris、Azide等殘留分子，純化後抗體溶液，可直接應用於lateral flow test奈米金抗體標誌套組 (Innova gold,#230-0010, innova biosciences)、與HRP/螢光抗體標誌套組(Innova lightning-link, #701-0000)。

Antibody Concentration and Clean-Up Kit (861-0010)

抗體濃縮及純化套組

AbSelect Antibody Purification System (860-0010)

------奈米金抗體標誌 (InnovaCoat)----

------HRP/螢光抗體標誌 (lightning-link)----

抗體濃縮及Clean up系統

適合應用: 低濃度抗體之純化及濃縮。

干擾酵素型HRP、Biotin、streptavidin及FITC螢光等之抗體標誌反應。移除溶液中殘留之amino group。純化後之抗體，可直接進行奈米金抗體標誌套組 (Innovagold,#230-0010, innova biosciences)及抗體螢光或HRP標誌(lightning_link)。

抗體濃縮及Clean up流程

Step 1 加入 0.5ml 以上的抗體量至 spin cartridge.

Step 2 15,000g離心 1 to 3 分, 使抗體量降低至100ul

Step 3 吸掉離心管的溶液

Step 4 加入 400μl conjugation buffer至spin
cartridge中的抗體

Step 5 15,000g離心 1 to 3 分, 使抗體量降低至100ul

Step 6吸掉離心管的溶液.

Step 7 重覆1-6步驟, 置換抗體溶液。

Step 8 由spin cartridge中回收純心後的抗體

Antibody Concentration and Cleanup Kit

Antibodies are sometimes only available at low concentrations and often contain low molecular weight substances that interfere in labeling reactions with enzymes, biotin, streptavidin and fluorophores. The Antibody Concentration and Clean Up Kit allows for the quick and easy concentration of antibodies and proteins. The kit can also be used to reduce the concentration of many unwanted additives often found in antibody formulations such as azide, glycine or tris.

The antibody clean up kit method utilizes a simple spin
column to easily and quickly remove excess buffer from the antibody thereby providing a more concentrated antibody solution. The clean up kit also allows the experimenter to perform a simple buffer exchange to transfer the antibody into a more favorable conjugation buffer.

DIY抗體標誌螢光(FITC,PE,Cy3,Cy5,Cy7 dye)

注意事項

請確認抗體的保存條件。傳統市售的抗體標誌套組，在使用前皆需dialysis純化，將抗體保存的物質移除。Lightning-link抗體標誌套組在大部份的保存條件下，不需要再dialysis的步驟，例如一般salts (e.g. NaCl)、sugars(e.g. sucrose)、及chelators (e.g. EDTA)不會影響標誌的活性。大部的市售的抗體保存在1%BSA、0.5%Azide及0.5% Gelatin 輕微或幾乎不會影響標誌的活性。在少數情況下，若是您購買的抗體保存在amino acids(e.g. glycine)、blockers(e.g. ethanolamine)及thiols(DTT, mercaptoethanol)情況下，記得純化您的抗體，(abselect抗體純化套組) 避免抑制您的標誌活性了!