太鼎生物科技/太鼎食安科技

ATPase分析套組---產品介紹

產品應用ATPases酵素活性之分析

內附有ATPase inhibitor。ATPase酵素活性分析，採用超高靈敏度Malachite green磷酸根呈色方法，克服傳統呈色方法,低靈敏度限制。測量目標樣本中，
ATPases酵素活性。

ATPase活性分析檢測

產品應用

Innova Biosciences 提供之ATPase分析套組，可探討酵素活性是否扮演transport、signal transduction,
protein biosynthesis及cell differentiation之角色。應用於ATPase活性分析。提供high-throughput screen 大量ATPase inhibitor。可建立快速藥物篩選系統。

ATPase活性分析檢測

產品特點

1.     
提供high-throughput screen 大量ATPase inhibitor。

2.     
ATPase酵素分析套組，包括stabilizing solution，可避開acid labile substrate所造成的高背景值，其背景值很低(
1mM )。

2. 其分析波段為600~660nm，避開一般colored compounds之干擾。

3. Pi-dye複合物極為穩定，其訊號強度可維持數小時之久。

4. Superior呈色系統。採用Malachite Green磷酸根呈色方式，呈色靈敏度比傳統呈色方法高，克服低靈敏度的限制。

5. 適用於大部份的assay buffer。

6. 蛋白質濃度高時，不會抑制顏色的形成。

訂購貨號# 601-0120,
196 assays (靈敏度4~40uM)

訂購貨號# 601-0121,
480 assays (靈敏度4~40uM)

訂購貨號# 601-0122,
480*5 assays (靈敏度4~40uM)

產品內容物

1 x 10ml of PiColorLock™ Gold* (1 x 25ml)

1 x 0.25ml of Accelerator (1 x 0.5ml)

1 x 5ml of Stabiliser (1 x 10ml)

1 x 1.5ml of 0.1M MgCl2 (2 x 1.5ml)

1 x 5ml of 0.5M Tris pH 7.5 (1 x 10ml)

1 x 5ml of 0.1mM Pi standard (1 x 10ml)

更多參考內容:

原廠連結: http://www.innovabiosciences.com/

相關商品

PiColorLock磷酸根分析檢測套組

http://tw.myblog.yahoo.com/taiding2000/article?mid=723&prev=724&next=715&l=f&fid=26

GTPase活性分析檢測套組

http://tw.myblog.yahoo.com/taiding2000/article?mid=724&prev=725&next=723&l=f&fid=26

游離磷酸根移除resin

5g
(501-0015)

Inorganic phosphate (Pi) is a
product of many enzymatic reactions, for example, those catalysed by
phosphatases and ATPases. Measurement of released Pi allows enzyme activity to
be determined, but contamination of enzymes or buffers by Pi can lead to unacceptably
high assay backgrounds.

PiBind™ resin provides a quick
and easy way to remove contaminating Pi from buffers. The resin works over a
broad range of pH values and is unaffected by many commonly used buffer
additives. PiBind™ resin can also be used to remove Pi from protein samples
(e.g. tissue extracts). This method is quicker than dialysis and unlike
desalting does not necessarily lead to a significant dilution of the sample.

ATPase酵素活性分析套組---文獻發表

ATPase酵素活性分析套組

ATPase Assay Kit

View
Product

• Caveolin-1 regulates proliferation and
  osteogenic differentiation of human mesenchymal stem cells
  - Journal of Cellular Biochemistry (2012)


• Common Pathogenic Effects of Missense Mutations
  in the P-Type ATPase ATP13A2 (PARK9) Associated with Early-Onset
  Parkinsonism - PLOS One (2012)


• Structure of the VirB4 ATPase, alone and bound
  to the core complex of a type IV secretion system
  - PNAS (2012)


• Peroxisomal proteostasis involves a Lon family
  protein that functions as protease and chaperone - JBC
  (2012)


• Xeroderma pigmentosum group F protein binds to
  Eg5 and is required for proper mitosis: implications for XP-F and XFE
  - Genes to Cells (2012)


• hSWS1·SWSAP1 Is an
  Evolutionarily Conserved Complex Required for Efficient Homologous
  Recombination Repair - JBC (2011)


• Enzyme Stability of Microencapsulated Bifidobacterium animalis ssp. lactis Bb12 after Freeze Drying and during Storage in
  Low Water Activity at Room Temperature - Journal
  of Food Science (2011)


• Digitoxin and a synthetic monosaccharide analog
  inhibit cell viability in lung cancer cells -
  Toxicology and Applied Pharmacology (2011)


• Functional
  defect caused by the 4544G>A SNP in ABCC2: potential impact for drug
  cellular disposition - Pharmacogenetics and
  Genomics (2011)


• A
  Common Variant in TFB1M
  Is Associated with Reduced Insulin Secretion and Increased Future Risk of
  Type 2 Diabetes - Cell Metabolism (2011)


• Overexpression of the Endoplasmic Reticulum
  Chaperone BiP3 Regulates XA21-Mediated Innate Immunity in Rice
  – PLoS One (2010)


• Identification of gp96 as a Novel Target for
  Treatment of Autoimmune Disease in Mice - PLoS One
  (2010)


• Antimicrobial
  Mechanism of Action of Transferrins: Selective Inhibition of H⁺-ATPase
  - Antimicrob Agents Chemother. (2010)


• Modulation of UvrD
  Helicase Activity by Covalent DNA-Protein Cross-links
  - Biol Chem. (2010)


• Monoclonal
  antibodies against nucleoside triphosphate hydrolase-II can reduce the
  replication of Toxoplasma gondii -
  Parasitology (2010)


• Biochemical Dissection
  of the ATPase TraB, the VirB4 Homologue of theEscherichia coli pKM101
  Conjugation Machinery -
  J. Bacteriol. (2010)


• Identification of
  gp96 as a Novel Target for Treatment of Autoimmune Disease in Mice
  – PLoS One (2010)


• ATP-binding
  cassette-like transporters are involved in the transport of lignin
  precursors across plasma and vacuolar membranes
  - PNAS (2010)


• Arabidopsis stromal 70-kDa heat shock proteins
  are essential for chloroplast development - Planta
  (2010)


• Lipopeptide induces apoptosis in fungal cells by
  a mitochondria-dependent pathway - Peptides (2010)


• Modulation of a
  Schistosoma mansoni multidrug transporter by the antischistosomal drug
  praziquantel - The FASEB Journal (2010)


• Biosynthesis of (Bacterio)chlorophylls
  ATP-Dependent Transient Subunit Interaction And Electron Transfer Of Dark
  Operative Protochlorophyllide Oxireductase - JBC
  (2010)


• Stress-Dependent Coordination of Transcriptome
  and Translatome in Yeast - PLoS Biology (2009)


• Crystal Structure
  of a Novel Conformational State of the Flavivirus NS3 Protein:
  Implications for Polyprotein Processing and Viral Replication - J Virol (2009)


• ATP and MO25α
  Regulate the Conformational State of the STRADα Pseudokinase and
  Activation of the LKB1 Tumour Suppressor – PLoS
  Biol (2009)


• Apratoxin A
  Reversibly Inhibits the Secretory Pathway by Preventing Cotranslational
  Translocation - Molecular Pharmacology
  (2009)


• NS4A
  regulates the ATPase activity of the NS3 helicase: a novel cofactor role
  of the non-structural protein NS4A from West Nile virus
  - Journal of General Virology (2009)


• Evidence That Two ATP-Dependent (Lon) Proteases
  in Borrelia burgdorferi
  Serve Different Functions - PLoS Pathogens
  (2009)


• ATP and MO25α Regulate the Conformational State
  of the STRADα Pseudokinase and Activation of the LKB1 Tumour Suppressor
  - PLoS Biology (2009)


• Stress-Dependent Coordination of Transcriptome
  and Translatome in Yeast - PLoS Biology (2009)


• An Afg2/Spaf-Related Cdc48-like AAA ATPase
  Regulates the Stability and Activity of the C. elegans Aurora B Kinase AIR-2 -
  Developmental Cell (2008)


• Staphylococcus aureus HrtA Is an ATPase Required for Protection
  against Heme Toxicity and Prevention of a Transcriptional Heme Stress
  Response - Journal of Bacteriology (2008)


• ATP
  scavenging by the intracellular pathogen Porphyromonas gingivalis inhibits
  P2X₇-mediated host-cell apoptosis
  - Cell Microbiol. (2008)


• The Two-component NS2B-NS3 Proteinase Represses
  DNA Unwinding Activity of the West Nile Virus NS3 Helicase
  - JBC (2008)


• Functional characterization of the nucleotide
  binding domain of the Cryptosporidium
  parvum CpABC4
  transporter: An iron–sulfur cluster transporter homolog
  - Molecular and Biochemical Parasitology (2008)


• Hippocampal
  levels of gamma-enolase, C-1-tetrahydrofolate synthase and serotransferrin
  fluctuate over the estrous cycle in the rat
  - Neuroscience (2008)


• Consumption of Soy Protein Isolate Modulates the
  Phosphorylation Status of Hepatic ATPase/ATP Synthase ß Protein and
  Increases ATPase Activity in Rats - J. Nutr.
  (2007)


• First identification and functional
  characterization of an immunogenic protein in unculturable haemotrophic
  Mycoplasmas (Mycoplasma
  suis HspA1)
  - FEMS Immunology & Medical Microbiology (2007)


• Identification,
  structure and mode of action of a new regulator of the Helicobacter pylori
  HP0525 ATPase - EMBO J. (2007)


• Proteome Profiling in
  the Rat Harderian Gland - J Proteome Res (2006)

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陳俊豪

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