TUNEL-based IHC staining kit K403-50

ApoBrdU-IHC DNA Fragmentation Assay Kit, K403-50

TUNEL是檢測因凋亡所產生DNA片段的一種常用方法。

Kit Summary:
• Sample type- Tissue sections and fixed cell preparations
• Species reactivity- Mammalian
• Applications- The TUNEL-based immunohistochemical staining kit provides complete reagents including positive and negative control slides for convenient detection of DNA fragmentation

Features & Benefits:
• Simple procedure; convenient
• The assay is sensitive and stable
• The assay offers an one-step labeling of apoptotic cells.

Kit components:
• Control Slides-pos/neg
• Blocking Buffer
• H
O/Urea Tablets
• Protease K
• DAB Tablets
• TdT Enzymes
• Br-dUTP
• 200X Conjugate
• 5X Reaction Buffer
• Anti-BrdU-Biotin mAb
• Methyl Green

Description:
Internucleosomal DNA fragmentation is a hallmark of apoptosis in mammalian cells. BioVision’s Apo-BrdU-IHCTM Kit is a two-color TUNEL (Terminal deoxynucleotide transferase dUTP Nick End Labeling) assay for labeling DNA breaks to detect apoptotic cells by immunohistochemistry. The kit contains positive/negative control slides for assessing reagent performance; reaction and blocking buffers for processing individual steps in the assay; proteinase K; terminal deoxynucleotidyl tranferase enzyme (TdT), bromodeoxyuridine triphosphate (Br-dUTP), biotin labeled antiBrdU antibody for labeling DNA breaks, horseradish peroxidase streptavidin conjugate, DAB, HO/Urea tablets for color generation and methyl green solution for counter staining the cells.

Storage Conditions:
-20°C

Shipping Conditions:
gel pack

USAGE: For Research Use Only! Not For Use in Humans.

商品如下連結

http://www.biovision.com/apobrdu-ihc-dna-fragmentation-assay-kit-3599.html

WWW.BIOVISION.COM

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