ECL冷光試劑 | SuperKine™ West Femto Maximum Sensitivity Substrate貨號BMU102-EN
SuperKine™ West Femto Maximum Sensitivity Substrate 提供了一種高度敏感的顯色溶液,可增強免疫學實驗的化學信號。為了獲得最佳的實驗結果,您需要優化所有實驗因素,包括樣品數量、抗體濃度(antibody concentration)、膜的使用和封閉劑 (blocking agents)。ECL 底物系列(ECL substrates)是一系列預混合(premixed)的即用型(ready-to-use)化學發光試劑(chemiluminescent reagents),用於檢測基於 HRP 的蛋白質(HRP-based Westerns)。 SuperKine™ West Femto 最大靈敏度底物專門針對免疫印跡上(picogram amounts) 的抗原檢測進行了優化。底物的強信號輸出和持續時間可以用照相或其他成像方法完美地檢測(picogram amounts)的抗原。
Product name |
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Kit components |
• ECL HRP Substrate Reagent A |
Features & Benefits |
The SuperKine™ West Femto Maximum Sensitivity Substrate developed by Abbkine is based on the innovation and optimization of the classic peroxidase substrate system. It is used for enhanced chemiluminescence (ECL) and can directly replace other expensive ECL products without the need to renew Optimize experimental conditions. |
Usage notes |
• Mix the two substrate components at a 1:1 ratio to prepare the substrate working solution. |
Storage instructions |
Stable for two years at 4-8°C from date of shipment. Exposure to the sun or any other intense light can harm the working solution. Short-term exposure to typical laboratory lighting will not harm the working solution. |
Shipping |
Gel pack with blue ice. |
活細胞和死細胞雙染色套組 | CCK-8細胞增殖和細胞毒性試劑盒 | LDH細胞毒性測定試劑盒 | 細胞衰老檢測試劑盒(β-半乳糖苷酶 | 細胞增殖EdU Image試劑盒
產品貨號 |
產品品項 |
反應 |
測量儀器 |
100個反應 |
活細胞綠色螢光染劑Ex/Em = 488/530 nm; 死細胞紅色螢光染料(Ex/Em = 535/617) |
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50個反應 |
螢光酶標儀、螢光顯微鏡或流式細胞儀。FITC通道(Ex / Em = 490nm / 520 nm) |
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500個反應 |
吸收光450nm |
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50個反應/250個反應 |
Ex / Em = 490/523 nm增殖(proliferating)和非增殖細胞(non-proliferating cells) |
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50個反應/250個反應 |
Ex/Em = 500/520 nm |
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100個反應/500個反應 |
Ex/Em = 484/501 nm |
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10MG/100MG |
Ex/Em: 358/461 nm (with DNA) |
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50個反應/250個反應 |
deep red fluorescent dye , Ex/Em:650/665nm |
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100個反應 |
光學顯微鏡 |
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1000個反應/10,000個反應 |
吸收光450nm |
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(BrdU替代法) |
100個反應/500個反應 |
AbFluor 488 azide (Ex/Em = 501/525 nm) |
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96個反應/ 192個反應 |
colorimetric (540 nm) 檢測範圍:1-100 µM |
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96個反應/ 480個反應 |
OD 450 nm 線性範圍15.6 -500 mU/ |
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2000個反應/ 5000個反應 |
OD 562 nm 相容性 5 % SDS, 5 % Triton X-100, 5 % Tween-20, 60, 80 50 to 1000 µg/ml. |
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96個反應/ 480個反應 |
λmax= 450 nm 1- 20 U/mL of LDH |
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200UG/1MG |
human IGF1 isoform 1 (P05019-1) (Gly 49-Ala 118) > 95 % as determined by SDS-PAGE |
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100個反應/500個反應 |
Ex/Em=535/615 nm. |
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1ml×5 3mlx5 |
More than 2,000,000 EU/mL to eliminate >99% of endotoxins. |
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48個反應/ 96個反應 |
吸收光370 nm |
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48個反應/ 96個反應 |
Superoxide anion scavenging rate D% =(∆∆ODControl - ∆∆ODSample)÷ ∆∆ODControl ×100% |
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48個反應/ 96個反應 |
吸收光 536nm Hydroxyl radical scavenging rate D% =(ASample - AControl)÷(ABlank - AControl)×100% |
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48個反應/ 96個反應 |
500nm Standard: 5 μmol/mL |
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48個反應/ 96個反應 |
吸收光505 nm Standard: 5 μmol/mL. |
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96個反應/ 192個反應 |
吸光值630nm 4- 300 mg/dL) |
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96個反應/ 480個反應 |
colorimetric (450 nm) 0.03 mM-2 mM |
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96個反應/ 96 X5盤 |
吸收光510 nm |
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96個反應/ 480個反應 |
593 nm 偵測範圍: 0.15-3 mM |
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20個反應/50個反應/100個反應 |
Annexin V- AbFluor™ 488: Abs/Em = 491/517 nm; PI: Abs/Em = 535/617 nm (with DNA) |
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96個反應/ 96 X5盤 |
450 nm 偵測範圍: 250pg/mL-16000 pg/mL, 最低靈敏度100 pg/mL |
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96個反應/ 96 X5盤 |
450 nm 偵測範圍40 pg/ml-1500 pg/ml |
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96個反應/ 96 X5盤 |
450 nm 7.8 pg/ml-500 pg/ml |
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96個反應/ 96 X5盤 |
450 nm 偵測範圍: 31.25 pg/mL-2000 pg/mL |
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96個反應/ 96 X5盤 |
Microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm. 偵測範圍: 15.6 pg/ml-1000 pg/ml |
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96個反應/ 96 X5盤 |
450 nm 偵測範圍: 22.5 ng/L-360 ng/L, 最低靈敏度1.0 ng/L。 |
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50ul/200ul |
WB (1:5000). |
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100ul/400ul/2ml |
GKPIPNPLLGLDST epitope |
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貨號KTA4001 |
100個反應/ 500個反應 |
(聚結的JC-1,Ex / Em = 585/590 nm)發出綠色螢光(JC-1單體,Ex / Em = 510/527 nm) JC-1 Stain CCCP (10 mM) Assay Buffer (5×) |
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96個反應 |
412 nm 2-200 µg/mL |
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1MG/10MG/100MG |
λEx/λEm: 358/461 nm (with DNA) |
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50個反應/ 200個反應 |
Cytoplasmic Solution A (CES A) Cytoplasmic Solution B (CES B) • Nuclear Extraction Solution (NES) DTT (500X) Protease Inhibitor (100X) |
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50ug/1mg/10mg |
λEX/λEm: 494/517 nm (pH 8) (calcein)
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96個反應 |
240nm |
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96個反應/ 480個反應 |
OD565 nm 96 孔板測定中的檢測限值為 0.78 μM 線性最高 50 μM NAD+/NADH。 |
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96個反應/ 480個反應 |
OD 450 nm 線性範圍15.6 -500 mU/mL |
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96個反應/ 480個反應 |
OD 540 nm 2-75 µM |
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96個反應 |
OD 664 nm 線性範圍為15.6-1000 µU/mL |
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脂質過氧化檢測試劑盒(MDA) |
96個反應 |
OD 532 nm 1-100 µM/L |
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96個反應 |
200-3.13 U/ml |
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96個反應/ 480個反應 |
OD 565 nm 10,00-100,0000 cells per well |
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48個反應/ 96個反應 |
absorption at 340 nm |
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48個反應/ 96個反應 |
absorbance at 270 nm 0.3125-20 μmoL/mL |
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48個反應/ 96個反應 |
absorbance at 760 nm 0.0156-1 mg/mL |
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48個反應/ 96個反應 |
Absorption peak at 500nm Detection range: 0.078-5 mmol/L(The Detection range corresponds to the standard, and the actual content of sample is 0.156-10 mmol/L) |
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48個反應/ 96個反應 |
Absorption peak at 500nm Detection range: 0.078-5 mmol/L(The Detection range corresponds to the standard, and the actual content of sample is 0.117-7.5 mmol/L) |
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48個反應/ 96個反應 |
absorption at 340 nm. |
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48個反應/ 96個反應 |
absorption at 412 nm. |
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48個反應/ 96個反應 |
absorption at 660 nm |
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5ug/20ug/100ug/1mg |
human IL-6 (NP_000591.1) (Val 30-Met 212)/ E. coli |
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20ug/100ug/1mg |
ED50 for this effect is 0.05-0.2 ng/mL. The specific activity of Recombinant Human IL-4 is approximately 3.2 × 10E4 IU/μg. |
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100ug/1mg |
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96個反應/ 480個反應 |
OD 580 nm : 1 µM-100 µM |
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48個反應/ 96個反應 |
absorption peak at 540nm 0.0125-0.5 mg/mL |