MoticEasyScan 全景玻片影像掃描器,3D多層次掃描,快速對焦精準掃描為數位玻片。提供最佳解析度0.25um/pixel ;6片式片夾,walk-away設計。搭配簡易操作軟體,優質影像數位永久保存。歡迎來電洽詢(02)86609496許虹宜

美國SouthernBiotech公司膠原蛋白及其抗體產品

關於膠原(Collagen):

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References 30秒抗體標誌螢光 HRP、Biotin、Avidin標誌套組
文獻發表

immLink
 

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Lightning-Link - the World's Easiest to Use Antibody Labeling Kits       (Direct labeling primary antibody)
 

Circumvents problems of cross over and non-specific binding of secondary reagents.
Reduces the number of incubation and tedious wash steps.
Delivers a unique opportunity to study multi-protein complexes using antibodies generated from either the same or different species.

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【如何移除免疫組織染色的背景值】
 
在做mouse組織切片時,很容易發生backgroud的問題。
尤其是冷凍切片的組織。Innovex Fc Receptor Block跟您的背景值說再見!!

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Innova biosciences AbSelect TCS Hybridoma單株抗體純化系統。高效能純化tissue culture supernatant (TCS)


immLink


【單株抗體經由融合瘤細胞株tissue culture supernatant (TCS)產製】


TCS本身包括許多雜質會干擾抗體後續下游分析,例如抗體標誌試驗。成功地抗體標誌要求單株抗體純化過程中須經由純化的步驟,innova biosciencesAbSelect TCS系統】提供快速且簡單純化單株抗體的方法。其原理為應用高效能且高純度Protein A結合於Agarose beads上,純化Hybridoma 上清液的IgG片段。 AbSelect TCS resin純化最多的抗體,經由簡單的wash步驟將非目標蛋白移除,再經由eluted步驟將單株抗體回收。


AbSelect TCS系統特點】


·      All components and buffers supplied


·      Purify antibody from 10 to 50mls of hybridoma TCS


AbSelect TCS (1 purification : 10 to 50ml tissue culture supernatant) (862-0030)


bSelect TCS (3 purifications : 10 to 50ml tissue culture supernatant) (862-0500)


Hybridoma cell linesAntibodies are often generated from hybridoma cell lines and supplied in tissue culture supernatant (TCS). TCS itself contains many substances that interfere with antibody labeling reactions. Successful labeling therefore requires antibodies to be purified prior to labeling. The AbSelect TCS system is a fast and simple method to purify these antibodies.


AbSelect TCS is prepared by coupling highly purified protein A to agarose beads which can be used to purify IgG fractions from hybridoma supernatants.  The method involves the capture of the antibody on the AbSelect TCS resin and the removal of unwanted substances using a simple wash procedure. The purified product is then eluted and neutralized.


The components of the AbSelect TCS are fully compatible with the Lightning-Link™ antibody labeling kits (available separately), which allows the purified antibody to be immediately labeled with an enzyme, fluorescent protein or fluorescent dye, with a hands-on time of under 30 seconds.


更多參考內容:


 


原廠連結: http://www.innovabiosciences.com/


 


WWW.BIOPIONEER.COM.TW


 太鼎生物科技有限公司(02)86609496


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Imm-Link】產品,可於Peptides 或有機化合物(organic compounds)等小分子進行標誌,其標誌的種類如BSAOvalbuminKeyhole Limpet Hemocyanin (KLH)。可應用於產製carrier proteins,誘發免疫系統產生大量的抗體。依不同的需要,可標誌於蛋白質上不同的位置 Sulfhydryl Carboxylamino

 

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Lightning-Link】全世界最容易進行的抗體標誌實驗 -
 the World's Easiest to Use Antibody Labeling Kits
 

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活細胞螢光染劑 Calcein AM,用於活細胞觀察及細胞侵入定量實驗, 1755-250

Catalog#: 1755-250  | Size: 5 x 50 µg
 

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【如何降低免疫組織染色過程中背景值 第一部份】
IHC過程中,若是使用biotinavidin的系統, 建議使用biotin/avidinblocking buffer在抗體反應之前,先移去內源性biotin/avidin。如此就不會有非一性binding的問題。原理:阻斷內源性biotin (rich in liver,kidney,spleen) 所導致背景值的問題 適用於任何humananimal組織 。

【如何降低免疫組織染色過程中背景值 第二部份】


一般一抗或二抗都有Fc region 組織內含有大量Fc receptorFc region會和Fc receptor結合,


所以造成組織切片會很髒, 尤其冷凍切片的組織Fc receptor更多。建議在抗體反應之前,先將Fc receptor blocker, 如此當加入抗體後,便不會和fc region結合。

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Phosphate Detection



 


Innova Biosciences PiColorLock 針對實驗初篩所設計,可探討酵素活性是否扮演transportsignal transduction, protein biosynthesiscell differentiation之角色。應用於ATPaseGTPaseProteinases活性分析。提供high-throughput screen 大量ATPaseGTPase inhibitor












Pi Colorlock (ALS)


PiColorLock is a phosphate detection reagent for measuring Phosphatases, ATPases and Nucleotidases.

 









Pi Colorlock (Gold)


PiColorLock is a phosphate detection reagent for measuring Phosphatases, ATPases and Nucleotidases.

 









High Throughput Colorimetric ATPase Assays


This non radioactive colorimetric assay kit uses a 96 well format, and all the necessary reagents are supplied for measuring enzyme activity and are ideal for high throughput drug screening.










High Throughput Colorimetric GTPase Assays


This non radioactive colorimetric assay kit uses a 96 well format, and all the necessary reagents are supplied for measuring enzyme activity and are ideal for high throughput drug screening.

 


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Innova Biosciences PiColorLock 針對實驗初篩所設計,可探討酵素活性是否扮演transportsignal transduction, protein biosynthesiscell differentiation之角色。應用於ATPaseGTPaseProteinases活性分析。提供high-throughput screen 大量ATPaseGTPase inhibitor

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