GPx穀胱甘肽過氧化物酶檢測 Glutathione Peroxidase (GPx/GSH-Px) Activity Assay Kit貨號 KTB1640
CheKineTM Micro Glutathione Peroxidase (GSH-Px) Activity Assay Kit
Glutathione peroxidase (GSH-Px),簡稱為GSH-Px或GPx
Determination of GSH-Px/GPx activity in serum, plasma, plant tissue/cell lysates and other biological fluids.
measuring absorbance at 340 nm
穀胱甘肽 (Glutathione) 研究的意義
穀胱甘肽是含有谷氨酸 (glutamate),半胱氨酸(cysteine)和甘氨酸(glycine)的三肽,其中含有巰基(sulfhydryl groups)。它廣泛存在於動植物中。它具有抗氧化劑(antioxidant)和綜合解毒(detoxification)作用。其相關的輔酶(coenzyme)是NADPH。人體新陳代謝產生的自由基(free radicals)過多會破壞生物膜(biofilm),侵入生命大分子,加速人體衰老(aging),並誘發腫瘤(tumors)或動脈粥樣硬化(atherosclerosis) 的產生。穀胱甘肽具有增強生物體的抗氧化功能,促進生長以及增強生物體對人體不利環境的影響的功能。它的主要生理作用是清除體內的自由基。它也是一種很好的解毒劑,可以減弱有毒物質的毒性並從體內清除有毒物質。穀胱甘肽還促進生長並提高人體免疫力。穀胱甘肽(Glutathione)不僅可以用作藥物,還可以用作功能性食品的基礎材料,並且廣泛用於功能性食品中,例如抗衰老(anti-aging),增強免疫力(enhancing immunity)和抗腫瘤作用(anti-tumor)。
GSH-Px is one of the main enzymes that catalyze the oxidation of reduced glutathione (GSH) in the glutathione redox cycle. GSH-Px not only specifically catalyzes the reaction between reduced glutathione and ROS to form oxidized glutathione GSSG, thereby protecting the biofilm from ROS damage and maintaining the normal function of cells; it also protects the liver and improves the body immunity, antagonizing the harmful effects of harmful metal ions on the body and increasing the body's ability to resist radiation.
產品介紹:
GSH-Px /GPx穀胱甘肽過氧化物酶, Glutathione peroxidase (GSH-Px),簡稱為GSH-Px或GPx。是在穀胱甘肽氧化還原循環中催化還原型穀胱甘肽(reduced glutathione , GSH)氧化的主要酶之一。 GSH-Px /GPx【穀胱甘肽過氧化物酶, Glutathione Peroxidase (GSH-Px)】不僅能特異性催化還原型穀胱甘肽與ROS之間的反應,形成氧化型穀胱甘肽GSSG,從而保護生物膜免受ROS破壞並維持細胞的正常功能。它還可以保護肝臟,提高人體免疫力,對抗有害金屬離子(metal ions)對人體的有害影響,並增強人體抵抗輻射(radiation)的能力。GSH-Px 【穀胱甘肽過氧化物酶, Glutathione Peroxidase (GSH-Px /GPx)】催化H2O2氧化GSH還原型穀胱甘肽(reduced glutathione , GSH),生成GSSG (氧化型穀胱甘肽GSSG)。穀胱甘肽還原酶(glutathione reductase , GR)催化NADPH還原GSSG(氧化型穀胱甘肽GSSG),從而再生GSH還原型穀胱甘肽(reduced glutathione , GSH),而NADPH氧化產生NADP +。 NADPH在340 nm處有一個特徵吸收峰,而NADP +沒有。通過測量340 nm處吸光度的降低速率來確定NADPH脫氫速率,以計算GSH-Px 【穀胱甘肽過氧化物酶, Glutathione Peroxidase (GSH-Px /GPx)】活性。
GSH-Px catalyzes H2O2 to oxidize GSH to produce GSSG; glutathione reductase (GR) catalyzes NADPH to reduce GSSG to regenerate GSH, while NADPH oxidizes to produce NADP+; NADPH has a characteristic absorption peak at 340 nm, while NADP+ does not; NADPH dehydrogenation rate is determined by measuring the decrease rate of absorbance at 340 nm to calculate GSH-Px activity.
| Product name | CheKineTM Micro Glutathione Peroxidase (GSH-Px /GPx) Activity Assay Kit |
| Applications notes | GSH-Px catalyzes H2O2 to oxidize GSH to produce GSSG; glutathione reductase (GR) catalyzes NADPH to reduce GSSG to regenerate GSH, while NADPH oxidizes to produce NADP+; NADPH has a characteristic absorption peak at 340 nm, while NADP+ does not; NADPH dehydrogenation rate is determined by measuring the decrease rate of absorbance at 340 nm to calculate GSH-Px activity. |
| Kit components | • Assay Buffer • Substrate • GR • H2O2 |
| Features & Benefits | • Determination of GSH-Px activity in serum, plasma, plant tissue/cell lysates and other biological fluids. |
| Usage notes | • 96 Well Clear Flat Bottom UV-Transparent Microplate is needed. • The whole processes need to be carried out on ice, and the enzyme activity should be determined on the same day, to avoid repeated freeze-thaw of the homogenate solution. • Prepare mixed reagents for use, and place on ice. • The measuring process should be operated quickly and only 1 2 blank well should be needed. • The reaction temperature has influence on the result. Keep the temperature at 25C (for general species) or 37C (for mammals). |
| Storage instructions | Storage at -20C and Keep from light immediately upon receipt. Kit has a storage time of 12 months from receipt. Refer to list of materials supplied for storage conditions of individual components. |
| Shipping | Gel pack with blue ice. |
| Precautions | The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product. |
| Background | GSH-Px is one of the main enzymes that catalyze the oxidation of reduced glutathione (GSH) in the glutathione redox cycle. GSH-Px not only specifically catalyzes the reaction between reduced glutathione and ROS to form oxidized glutathione GSSG, thereby protecting the biofilm from ROS damage and maintaining the normal function of cells; it also protects the liver and improves the body immunity, antagonizing the harmful effects of harmful metal ions on the body and increasing the body's ability to resist radiation. |
| 產品貨號 | CheKine產品品項 | CheKine產品原理 |
| KTB1600 |
英文名稱: CheKineTM Reduced Glutathione (GSH) Colorimetric Assay Kit |
還原型穀胱甘肽(GSH)比色分析試劑盒 (KTB1600)利用DTNB與還原型穀胱甘肽反應形成黃色產物。在412 nm處測得的光密度可以直接反映樣品中的穀胱甘肽濃度。 |
| KTB1610 |
英文名稱: CheKineTM Glutathione Oxidized (GSSG) Colorimetric Assay Kit |
穀胱甘肽氧化(GSSG)比色分析試劑盒:還原型穀胱甘肽可與DTNB反應並生成2-硝基-5-巰基苯甲酸,在412nm波長處具有最大的光吸收率。 |
| KTB1620 |
英文名稱: CheKineTM Glutathione Reductases (GR) Colorimetric Assay Kit |
穀胱甘肽還原酶(GR)比色分析試劑盒:GR可以催化NADPH還原為GSSG,從而再生GSH,NADPH脫氫生成NADP +;NADPH在340 nm有一個特徵吸收峰,而NADP +在該波長沒有吸收峰。通過測量340 nm處吸光度的降低率來計算GR活性,從而確定NADPH脫氫率。 |
| KTB1630 |
英文名稱: CheKineTM Glutathione S-Transferase (GST) Colorimetric Assay Kit |
穀胱甘肽S-轉移酶(GST)比色分析試劑盒:GST催化GSH與CDNB的結合。共軛伴隨著在340nm處吸光度的增加。增加速率與樣品中的GST活性成正比。 |
| KTB1640 |
英文名稱: CheKineTM Glutathione Peroxidase (GSH-Px/GPx) Colorimetric Assay Kit |
穀胱甘肽過氧化物酶(GSH-Px)比色測定試劑盒:GSH-Px催化H2O2氧化GSH生成GSSG;穀胱甘肽還原酶(GR)催化NADPH還原GSSG從而再生GSH,而NADPH氧化產生NADP +。 NADPH在340 nm處有一個特徵吸收峰,而NADP +沒有。通過測量340 nm處吸光度的降低速率來確定NADPH脫氫速率,以計算GSH-Px活性。 |
| KTB1650 |
英文名稱: CheKineTM Thioredoxin Reductase (TrxR) Colorimetric Assay Kit |
硫氧還蛋白還原酶(TrxR)比色測定試劑盒:TrxR可以催化NADPH還原DTNB從而生成TNB和NADP +。 TNB在412nm處具有特徵吸收峰。 TrxR活性可以通過測量412 nm處TNB的增加速率來計算。 |
| KTB1660 |
英文名稱: CheKineTM Thioredoxin Peroxidase (TPX) Colorimetric Assay Kit |
硫氧還蛋白過氧化物酶(TPX)比色測定試劑盒:TPX催化H2O2氧化二硫蘇糖醇(DTT)。 H2O2的吸收波長為240nm。 TPX活性可以通過測量240nm處吸光度的降低速率並減去過氧化氫酶(CAT)催化的H2O2來計算。因此,該試劑盒可以同時測量樣品的TPX和CAT活性。 |
活細胞和死細胞雙染色套組 CCK-8細胞增殖和細胞毒性試劑盒 LDH細胞毒性測定試劑盒 細胞衰老檢測試劑盒(β-半乳糖苷酶 細胞增殖EdU Image試劑盒 Abbkine 細胞膜染色 活細胞Lysosome染色
| Product Name | Catalog No |
| CheKineTM Coenzyme II NADP(H) Colorimetric Assay Kit | KTB1010 |
| CheKineTM Glucose-6-Phosphate Dehydrogenase (G6PDH) Activity Colorimetric Assay Kit | KTB1011 |
| CheKineTM Coenzyme I NAD(H) Colorimetric Assay Kit | KTB1020 |
| CheKineTM NAD+ kinase (NADK) Activity Colorimetric Assay Kit | KTB1022 |
| CheKineTM Superoxide Dismutases (SOD) Activity Colorimetric Assay Kit | KTB1030 |
| CheKineTM Catalase (CAT) Activity Colorimetric Assay Kit | KTB1040 |
| CheKineTM Lipid Peroxidation (MDA) Colorimetric Assay kit | KTB1050 |
| CheKineTM Xanthine Oxidase Activity Colorimetric Assay Kit | KTB1070 |
| CheKineTM Superoxide anion Scavenging Capacity Colorimetric Assay Kit | KTB1080 |
| CheKineTM Lactate Colorimetric Assay Kit | KTB1100 |
| CheKineTM Lactate Dehydrogenase (LDH) Colorimetric Assay Kit | KTB1110 |
| CheKineTM Glucose Colorimetric Assay Kit | KTB1300 |
| CheKineTM Glucose Oxidase Activity (GOD) Colorimetric Assay Kit | KTB1310 |
| CheKineTM Hydrogen Peroxide (H2O2) Colorimetric Assay Kit | KTB1041 |
| CheKineTM Nitric Oxide (NO) Colorimetric Assay Kit | KTB1400 |
| CheKineTM Total Antioxidant Capacity (TAC) Colorimetric Assay Kit | KTB1500 |
| CheKineTM Reduced Glutathione (GSH) Colorimetric Assay Kit | KTB1600 |
| CheKineTM Glutathione Oxidized (GSSG) Colorimetric Assay Kit | KTB1610 |
| CheKineTM Glutathione Reductases (GR) Activity Colorimetric Assay Kit | KTB1620 |
| CheKineTM Glutathione S-Transferase (GST) Colorimetric Assay Kit | KTB1630 |
| CheKineTM Glutathione Peroxidase (GSH-Px) Activity Colorimetric Assay Kit | KTB1640 |
| CheKineTM Thioredoxin Reductase (TrxR) Colorimetric Assay Kit | KTB1650 |
| CheKineTM Thioredoxin Peroxidase (TPX) Colorimetric Assay Kit | KTB1660 |
| CheKineTM NADH Oxidase (NOX) Colorimetric Assay Kit | KTB1021 |
| CheKineTM Pyruvate Kinase (PK) Colorimetric Assay Kit | KTB1120 |
| CheKineTM Protein Carbonyl Colorimetric Assay Kit | KTB1200 |
| CheKineTM Tissue and Blood Alkaline Phosphatase (AKP/ALP) Activity Colorimetric Assay Kit | KTB1700 |
| CheKineTM Na+/K+-ATPase Activity Colorimetric Assay Kit | KTB1800 |
| CheKineTM Ca2+/Mg2+-ATPase Activity Colorimetric Assay Kit | KTB1810 |
| CheKineTM Monoamine Oxidase (MAO) Activity Colorimetric Assay Kit | KTB1900 |
| CheKineTM Polyphenol Oxidase (PPO) Activity Colorimetric Assay Kit |
KTB1140 |
| CheKineTM Serum Potassium (K+) Colorimetric Assay Kit | KTB2100 |
| CheKineTM Triglyceride (TG) Colorimetric Assay Kit | KTB2200 |
| CheKineTM Free Cholesterol (FC) Colorimetric Assay Kit | KTB2210 |
| CheKineTM Total Cholesterol (TC) Colorimetric Assay Kit | KTB2220 |
| CheKineTM Pyruvate Acid (PA) Colorimetric Assay Kit | KTB1121 |
| CheKineTM Alanine Aminotransferase (ALT/GPT) Activity Colorimetric Assay Kit | KTB1410 |
| CheKineTM Aspartate Aminotransferase (AST/GOT) Activity Colorimetric Assay Kit | KTB1420 |
| CheKineTM Uric Acid (UA) Colorimetric Assay Kit | KTB1510 |
| CheKineTM Phosphoenolpyruvate Carboxylase (PEPC) Activity Colorimetric Assay Kit | KTB1122 |
| CheKineTM Toal Glutathione (T-GSH) Colorimetric Assay Kit | KTB1670 |
| CheKineTM Cysteine (Cys) Colorimetric Assay Kit | KTB1450 |
| CheKineTM Phenylalanine Ammonia Lyase (PAL) Activity Colorimetric Assay Kit | KTB1160 |
| CheKineTM Proline (PRO) Colorimetric Assay Kit | KTB1430 |
| CheKineTM Glutamate (Glu) Colorimetric Assay Kit | KTB1440 |
| CheKineTM γ-Glutamyl Cysteine Ligase (GCL) Activity Colorimetric Assay Kit | KTB1680 |
| CheKineTM Gamma-Glutamyl Transpeptidase (GGT) Activity Colorimetric Assay Kit | KTB1690 |
| CheKineTM Peroxidase (POD) Activity Colorimetric Assay Kit | KTB1150 |
| CheKineTM Plant Soluble Sugar Colorimetric Assay Kit | KTB1320 |
| CheKineTM Plant Flavonoids Colorimetric Assay Kit | KTB1530 |
| CheKineTM Plant Oligomeric Proantho Cyanidins (OPC) Colorimetric Assay Kit | KTB1520 |
| CheKineTM Plant Total Phenols (TP) Colorimetric Assay Kit | KTB1540 |
| CheKineTM Superoxide Anion Colorimetric Assay Kit | KTB1210 |
| CheKineTM Serum Sodium Colorimetric Assay Kit | KTB2130 |
| CheKineTM Serum Zinc Colorimetric Assay Kit | KTB2140 |
| CheKineTM Mitochondrial complex I Activity Assay Kit (colorimetric) | KTB1850 |
| CheKineTM Mitochondrial complexII Activity Assay Kit (colorimetric) | KTB1860 |
| CheKineTM Mitochondrial complex III Activity Assay Kit (colorimetric) | KTB1870 |
| CheKineTM Mitochondrial complex IV Activity Assay Kit (colorimetric) | KTB1880 |
| CheKineTM Mitochondrial complex V Activity Assay Kit (colorimetric) | KTB1890 |
| CheKineTM Citrate Synthase (CS) Activity Assay Kit( Colorimetric) | KTB1023 |
| CheKineTM Total Iron-Binding Capacity (TIBC) Colorimetric Assay Kit | KTB2150 |
| CheKineTM Pyruvate Dehydrogenase (PDH) Activity Assay Kit (Colorimetric) | KTB1270 |
| CheKineTM Reactive Oxygen Species (ROS) Detection Fluorometric Assay Kit | KTB1910 |
| CheKineTM Acetyl Coenzyme A (Acetyl-CoA) Colorimetric Assay Kit | KTB1260 |
| CheKineTM Diamine Oxidase (DAO) Activity Colorimetric Assay Kit | KTB1220 |
| CheKineTM Succinate Dehydrogenase(SDH) Activity Assay Kit (Colorimetric) | KTB1230 |
| CheKineTM α-Ketoglutarate Dehydrogenase (α-KGDH) Assay Kit (Colorimetric) | KTB1240 |
| CheKineTM Mitochondrial Isocitrate Dehydrogenase(ICDHm)Activity Colorimetric Assay Kit | KTB1250 |
| CheKineTM Blood Glucose Assay Kit(Colorimetric) | KTB1330 |
| CheKineTM Glycogen Assay Kit(Colorimetric) | KTB1340 |
| CheKineTM Total Carbohydrate Assay Kit(Colorimetric) | KTB1350 |
| CheKineTM Reducing Sugar(RS) Assay Kit(Colorimetric) | KTB1360 |
| CheKineTM Ceruloplasmin Activity Assay Kit ( Colorimetric) | KTB1550 |
| CheKineTM Advanced Oxidation Protein Products (AOPP) Assay Kit(Colorimetric) | KTB1060 |
| CheKineTM Hydroxyl Free Radical Scavenging Capacity Colorimetric Assay Kit | KTB1091 |
| CheKineTM Micro Hexokinase (HK) Activity Colorimetric Assay Kit | KTB1123 |
| CheKineTM 6-Phosphofructokinase(PFK) Activity Colorimetric Assay Kit | KTB1124 |
| CheKineTM Amino Acid (AA) Colorimetric Assay Kit | KTB1460 |
| CheKineTM Pyruvate Decarboxylase (PDC) Colorimetric Assay Kit | KTB1125 |
| CheKineTM Ribulose Bisphosphate Carboxylase/Oxygenase (Rubisco) Colorimetric Assay Kit | KTB1480 |
| CheKineTM Lysine (LYS) Colorimetric Assay Kit | KTB1470 |
| CheKineTM Free Fat Acid (FFA) Colorimetric Assay Kit | KTB2230 |
| CheKineTM α-Amylase Activity Colorimetric Assay Kit | KTB1370 |
| CheKineTM β-Amylase Activity Colorimetric Assay Kit | KTB1380 |
| CheKineTM Starch Branching Enzyme(SBE) Activity Colorimetric Assay Kit | KTB1390 |
| CheKineTM Acetylcholinesterase (AchE) Activity Colorimetric Assay Kit | KTB1710 |
| CheKineTM Oxygen Radical Antioxidant Capacity (ORAC) Fluorometric Assay Kit | KTB1501 |
| CheKineTM Hydroxyl Radical Antioxidant Capacity (HORAC) Fluorometric Assay Kit | KTB1502 |
| CheKineTM Alcohol Acyltransferase(AAT)Activity Colorimetric Assay Kit | KTB1560 |
| CheKineTM Fatty Acid Synthetase(FAS) Activity Colorimetric Assay Kit | KTB2240 |
| CheKineTM Aconitase (ACO) Activity Colorimetric Assay Kit | KTB1290 |
| CheKineTM Mitochondrial Malate Dehydrogenase(mMDH) Activity Colorimetric Assay Kit | KTB1280 |
| CheKineTM Low Density Lipoprotein Cholesterol (LDL-C) Colorimetric Assay Kit | KTB2260 |
| CheKineTM High Density Lipoprotein Cholesterol (HDL-C) Colorimetric Assay Kit | KTB2250 |
| CheKineTM Tannin Colorimetric Assay Kit | KTB1541 |
| CheKineTM Tannase(TAN) Activity Colorimetric Assay Kit | KTB1542 |
| CheKineTM Creatine Kinase (CK) Activity Colorimetric Assay Kit | KTB1012 |
| CheKineTM 6-Phosphogluconate Dehydrogenase Activity Colorimetric Assay Kit | KTB1013 |
| CheKineTM Anthocyanidin Reductase(ANR) Activity Colorimetric Assay Kit | KTB3010 |
| 產品貨號 | 品項 | 反應 | 測量儀器 |
| 貨號KTA1001 | 活細胞和死細胞雙染色試劑盒 | 100個反應 |
活細胞綠色螢光染劑Ex/Em = 488/530 nm; 死細胞紅色螢光染料(Ex/Em = 535/617) |
|
|
|
50個反應 | 螢光酶標儀、螢光顯微鏡或流式細胞儀。FITC通道(Ex / Em = 490nm / 520 nm) |
|
|
CCK-8細胞增殖和細胞毒性試劑盒 | 500個反應 | 吸收光450nm |
| 貨號KTC4003 | TraKineTM粒線體染色 | 50個反應/250個反應 | Ex / Em = 490/523 nm增殖(proliferating)和非增殖細胞(non-proliferating cells) |
| 貨號KTC4100 | Pro活細胞微管蛋白染色 | 50個反應/250個反應 | Ex/Em = 500/520 nm |
| 貨號KTC4001 | Abbkine 細胞膜染色 | 100個反應/500個反應 | Ex/Em = 484/501 nm |
| 貨號BMD00063 | DAPI Staining Solution | 10MG/100MG | Ex/Em: 358/461 nm (with DNA) |
| 貨號KTC4210 | 活細胞Lysosome染色 | 50個反應/250個反應 | deep red fluorescent dye , Ex/Em:650/665nm |
| 貨號KTA3030 | 細胞衰老檢測試劑盒(β-半乳糖苷酶) | 100個反應 | 光學顯微鏡 |
| 貨號KTA1020 | CCK-8細胞增殖和細胞毒性試劑盒 | 1000個反應/10,000個反應 | 吸收光450nm |
| 貨號KTA2030 |
(BrdU替代法) |
100個反應/500個反應 | AbFluor 488 azide (Ex/Em = 501/525 nm) |
| 貨號KTB1400 | 一氧化Dan(NO)檢測試劑盒 |
96個反應/ 192個反應 |
colorimetric (540 nm) 檢測範圍:1-100 μM |
| 貨號KTB1070 | CheKineTM黃嘌呤氧化酶檢測 |
96個反應/ 480個反應 |
OD 450 nm 線性範圍15.6 -500 mU/ |
| 貨號KTD3001 | BCA蛋白定量試劑盒 |
2000個反應/ 5000個反應 |
OD 562 nm 相容性 5 % SDS, 5 % Triton X-100, 5 % Tween-20, 60, 80 50 to 1000 μg/ml. |
| 貨號KTB1110 | 乳酸脫氫酶(LDH)測定試劑盒 |
96個反應/ 480個反應 |
λmax= 450 nm 1- 20 U/mL of LDH |
| 貨號PRP100153 | IGF-I重組蛋白 | 200UG/1MG |
human IGF1 isoform 1 (P05019-1) (Gly 49-Ala 118) > 95 % as determined by SDS-PAGE |
| 貨號 KTA2020 | 細胞週期染色檢測 | 100個反應/500個反應 | Ex/Em=535/615 nm. |
| 貨號BMC21400 | PurKineTM內毒素去除預裝離心柱 |
1ml5 3mlx5 |
More than 2,000,000 EU/mL to eliminate >99% of endotoxins. |
| 貨號KTA1200 | CheKineTM蛋白羰基比色測定試劑盒 |
48個反應/ 96個反應 |
吸收光370 nm |
| 貨號KTB1080 | 超氧陰離子清除測定試劑盒 |
48個反應/ 96個反應 |
Superoxide anion scavenging rate D% =(ODControl - ODSample) ODControl 100% |
| 貨號KTB1090 | 清除氫氧自由基能力測定試劑 |
48個反應/ 96個反應 |
吸收光 536nm Hydroxyl radical scavenging rate D% =(ASample - AControl)(ABlank - AControl)100% |
| 貨號KTB2220 | TC總膽固醇檢測試劑盒 |
48個反應/ 96個反應 |
500nm Standard: 5 μmol/mL |
| 貨號KTB1510 | CheKineTM尿酸(UA)比色分析試劑盒 |
48個反應/ 96個反應 |
吸收光505 nm Standard: 5 μmol/mL. |
| 貨號KTB1300 | Glucose葡萄糖測定試劑盒 |
96個反應/ 192個反應 |
吸光值630nm 4- 300 mg/dL) |
| 貨號KTB1100 | Lactate 乳酸檢測試劑盒 |
96個反應/ 480個反應 |
colorimetric (450 nm) 0.03 mM-2 mM |
| 貨號KTB1700 | 組織和血液鹼性磷酸酶( Alkaline Phosphatase, AKP / ALP)比色測定 |
96個反應/ 96 X5盤 |
吸收光510 nm |
| 貨號KTB1500 | 總抗氧化劑能力TAC分析 |
96個反應/ 480個反應 |
593 nm 偵測範圍: 0.15-3 mM |
| 貨號KTA0002 | Annexin V-AbFluorTM488細胞凋亡檢測試劑盒 | 20個反應/50個反應/100個反應 | Annexin V- AbFluorTM 488: Abs/Em = 491/517 nm; PI: Abs/Em = 535/617 nm (with DNA) |
| 貨號KET7013 | EliKineTM Rat IL-6 ELISA Kit試劑盒 |
96個反應/ 96 X5盤 |
450 nm 偵測範圍: 250pg/mL-16000 pg/mL, 最低靈敏度100 pg/mL |
| 貨號KET0003 | 雌二醇(Estradiol , E2)試劑盒 |
96個反應/ 96 X5盤 |
450 nm 偵測範圍40 pg/ml-1500 pg/ml |
| 貨號KET6032 | TNF-α Alpha型腫瘤壞死因子 ELISA試劑盒 |
96個反應/ 96 X5盤 |
450 nm 7.8 pg/ml-500 pg/ml |
| 貨號KET6033 | VEGF血管內皮生長因子ELISA試劑盒 |
96個反應/ 96 X5盤 |
450 nm 偵測範圍: 31.25 pg/mL-2000 pg/mL |
| 貨號KET7005 | 白介素-1β(Interleukin-1β, IL-1β)ELISA試劑盒 |
96個反應/ 96 X5盤 |
Microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm. 偵測範圍: 15.6 pg/ml-1000 pg/ml |
| 貨號KTE70003 | 小鼠黃嘌呤脫氫酶/氧化酶(XDH)ELISA試劑盒 |
96個反應/ 96 X5盤 |
450 nm 偵測範圍: 22.5 ng/L-360 ng/L, 最低靈敏度1.0 ng/L。 |
| 貨號 A02051HRP | His-tag標籤多株抗體-HRP | 50ul/200ul | WB (1:5000). |
| 貨號A02170MGB | Anti-V5 Tag Mouse Monoclonal Antibody (11D5), 磁珠 | 100ul/400ul/2ml | GKPIPNPLLGLDST epitope |
| 貨號KTA4001 | JC-1線粒體膜電位測定試劑盒 |
100個反應/ 500個反應 |
(聚結的JC-1,Ex / Em = 585/590 nm)發出綠色螢光(JC-1單體,Ex / Em = 510/527 nm) JC-1 Stain CCCP (10 mM) Assay Buffer (5) |
| 貨號KTB1600 | 穀胱甘肽(Glutathione)檢測 | 96個反應 |
412 nm 2-200 μg/mL |
| 貨號BMD00063 | DAPI Staining Solution | 1MG/10MG/100MG | λEx/λEm: 358/461 nm (with DNA) |
| 貨號KTP3001 | 細胞核/細胞質分離試劑盒 |
50個反應/ 200個反應 |
Cytoplasmic Solution A (CES A) Cytoplasmic Solution B (CES B) • Nuclear Extraction Solution (NES) DTT (500X) Protease Inhibitor (100X) |
| 貨號BMD00064-50UG | Calcein AM鈣黃綠素活細胞螢光染劑 | 50ug/1mg/10mg |
λEX/λEm: 494/517 nm (pH 8) (calcein)
|
| 貨號 KTB1660 | 硫氧還蛋白過氧化物酶(TPX)檢測 | 96個反應 | 240nm |
| 貨號KTB1020 | NAD/NADH 分析試劑盒 |
96個反應/ 480個反應 |
OD565 nm 96 孔板測定中的檢測限值為 0.78 μM 線性最高 50 μM NAD+/NADH。 |
| 貨號KTB1070 | CheKineTM黃嘌呤氧化酶檢測 |
96個反應/ 480個反應 |
OD 450 nm 線性範圍15.6 -500 mU/mL |
| 貨號KTB1040 | Catalase過氧化氫酶活性檢測試劑盒 |
96個反應/ 480個反應 |
OD 540 nm 2-75 μM |
| 貨號KTB1060 | 過氧化物酶(Peroxidases)分析試劑盒 | 96個反應 |
OD 664 nm 線性範圍為15.6-1000 μU/mL |
| 貨號KTB1050 | 脂質過氧化檢測試劑盒(MDA) | 96個反應 |
OD 532 nm 1-100 μM/L |
| 貨號KTB1030 | 超氧化物歧化酶(SOD)活性測定試劑盒 | 96個反應 | 200-3.13 U/ml |
| 貨號KTA1030 | LDH細胞毒性測定試劑盒 |
96個反應/ 480個反應 |
OD 565 nm 10,00-100,0000 cells per well |
