(AST/GOT) 活性測定試劑盒 | CheKine™ Micro Aspartate Aminotransferase 貨號KTB1420
天冬氨酸氨基轉移酶(Aspartate aminotransferase, AST/GOT）廣泛存在於動物、植物、微生物和培養細胞中。它催化可逆的氨基反應( reversible amino reaction )，是氨基酸代謝(amino acid metabolism)的重要酶。血清AST活性(Serum AST activity)水平是肝損傷(liver damage)的重要生化指標( biochemical indicator )，可以反映肝損傷(liver damage)的嚴重程度。此外，天冬氨酸氨基轉移酶(Aspartate aminotransferase, AST/GOT）在心肌細胞中含量最高，臨床上一般作為心肌梗塞(cardiomyocytes)、心肌炎( myocardial infarction)的輔助檢查。該試劑盒提供了一種檢測動物組織、植物組織、細胞培養物：貼壁或懸浮細胞、血清、細菌等多種生物樣品中 AST/GOT 活性的簡單方法。在該測定中，天冬氨酸氨基轉移酶 (AST) 在 37°C 和 pH 7.4 下催化 α-酮戊二酸(α-ketoglutarate)和 L-天冬氨酸(L-aspartic acid )的轉氨基反應(transamination reaction)，生成谷氨酸( glutamic acid)和丙酮酸(pyruvate)；丙酮酸(pyruvate)能與2,4-二硝基苯肼( 2,4- Dinitrophenylhydrazine )作用生成丙酮酸苯腙(pyruvate phenylhydrazone)，在鹼性條件( alkaline conditions )下呈棕紅色，在505nm處有特徵吸收峰。丙酮酸苯腙(pyruvate phenylhydrazone)在 505 nm 處的增加速率可以反映 AST/GOT 活性。
| Product name | CheKine™ Micro Aspartate Aminotransferase (AST/GOT) Activity Assay Kit |
| Applications notes | The kit provides a simple method for detecting AST/GOT activity in a variety of biological samples such as animal tissues, plant tissues, cell culture: adherent or suspension cells, serum, bacterial. In the assay, Aspartate aminotransferase (AST) catalyzes the transamination reaction of α-ketoglutarate and L-aspartic acid at 37°C and pH 7.4 to produce glutamic acid and pyruvate; Pyruvate can interact with 2,4- Dinitrophenylhydrazine to produce pyruvate phenylhydrazone, which appears brownish red under alkaline conditions and has a characteristic absorption peak at 505 nm. The rate of pyruvate phenylhydrazone increase at 505 nm can reflect AST/GOT activity. |
| Alternative | AST; GOT; Aspartate aminotransferase |
| Kit components | • Extraction Buffer • Reagent I • Reagent II • Reagent III • Standard |
| Features & Benefits | • Determination of AST/GOT activity in a variety of biological samples such as animal tissues, plant tissues, cell culture: adherent or suspension cells, serum, bacterial. • Detailed sample preparation and result calculation methods are provided. • Simple operation steps. • Standard and standard curve line are provided. |
| Usage notes | • Do not mix or substitute reagents or materials from other kit lots or vendors. • The sample preparation must be done on ice to prevent the enzyme inactivation, and the enzyme activity should be determined on the same day. avoid repeated freeze-thaw. • Ensure all reagents and equipment are at the appropriate temperature before starting the assay. |
| Storage instructions | Storage at 4°C and protected from light upon receipt. Kit has a storage time of 6 months form receipt. |
| Shipping | Gel pack with blue ice. |
| Background | Aspartate aminotransferase (AST/GOT) is widely present in animals, plants, microorganisms and cultured cells. It catalyzes the reversible amino reaction and is an important enzyme for amino acid metabolism. Serum AST activity level is an important biochemical indicator of liver damage, which can reflect the severity of liver damage. In addition, AST has the highest content in cardiomyocytes and is generally used as an auxiliary examination for myocardial infarction and myocarditis in clinical practice. |
| Alternative | AST; GOT; Aspartate aminotransferase |
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