~【NEW!!活動快報】~ 全景玻片影像掃描器,精準對焦與掃描,染色結果從此數位化
MoticEasyScan 全景玻片影像掃描器,3D多層次掃描,快速對焦精準掃描為數位玻片。提供最佳解析度0.25um/pixel ;6片式片夾,walk-away設計。搭配簡易操作軟體,優質影像數位永久保存。歡迎來電洽詢(02)86609496許虹宜

目前分類:【TMAs組織晶片、IHC免疫組織染色】 (9)

瀏覽方式: 標題列表 簡短摘要

Motic EASYSCAN 全景玻片掃描器

Motic EASYSCAN提供高解析度、高通量、低門檻的玻片掃瞄系統

EZSCAN-spec.png

 

Motic EASYSCAN 儀器特點

  • 1. 使用高NA物鏡,提供最佳解析度 (0.25μm/pixel)
  • 2. 6片式片夾,walk-away設計
  • 3. 提供2種相機選擇
  • 4. 高速、高精準自動對焦
  • 5. 3D多層次掃描
  • 6. 多種掃描模式,滿足每一個樣品需求
  • 7. 提供完整的切片分享解決方案
  • 8. 檔案格式相容於多種虛擬玻片觀看軟體

-----------------------------------------

6片式片夾,Walk-away自動化設計

Ezscan2-300x240.png

高NA物鏡,提供高解析度掃描結果

Ezscan3-300x261.png

------------------------------------

台灣代理廠商: 太鼎生物科技有限公司
公司電話: 02-86609496 /0920312382
公司傳真: 02-86609342
公司網站: www.biopioneer.com.tw
連絡業務: 許虹宜 0920312382
連絡信箱: taiding.biotech@msa.hinet.net

文章標籤

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NEGATIVE CONTROL SERA


Negative control sera and isotype controls are employed in place of primary antibody for staining negative control slides. Negative control slides void of staining verifies the staining specificity of the primary antibody for the target antigen as stained in positive control and case slides.



Innovex Biosciences offers a wide selection of negative control sera and isotype controls for different sources of primary antibodies employed.



Negative Control Sera


Please click this icon to download the PDF file.




















































Item# Product SizePrice

NCP801R

Rabbit

7 ml

$95



NCM802M

Mouse (ascites fluid)

7 ml

$95



NC909

Sheep

7 ml

$95



NC912

Goat

7 ml

$89



NC914

Chicken

7 ml

$175



NC915

Rat

7 ml

$165


    

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SIGNAL ENHANCING WASH BUFFERS


A Room temperature retrieval
and stain amplification without pre-treatments










AEC staining of UCHL-1 (T cell) antibody of tonsil paraffin section rinsed with PBS









Same section retrieved when simply rinsed with Innovex Enhancing Wash Buffer - No pre-treatments





ADVANCED FEATURES
• Simple to use, employ as the rinse buffer in place of PBS for rinsing in between incubation steps
• Amplify staining signal by 2-4 folds with no additional steps added to the immunostaining procedure
• Cost effective: Allows 2-4 folds increase for primary antibody dilution
• Time Saver : Allows for decrease in primary antibody incubation time
• Allows for decrease in chromogen/substrate incubation time
• Fixation independent; Amplifies staining regardless of the type of fixative employed
• Eliminates enzyme pre-digestion
• Minimizes the need for high heat antigen recovery when used with INNOVEX Staining Systems
• Minimizes false negative staining
• Automation compatible
• Applicable to ELISA and flow cytometric procedures



USER FRIENDLY PROTOCOL
Innovex Enhancing Wash Buffers only require 5 seconds rinse in between incubation steps when used with Innovex STAT-Q and HISTO-STAT ( 2-step) staining systems. Innovex Enhancing Wash Buffers may be employed with other systems, observe the manufacturer’s instruction for the required time and number of washes recommended for the system.



1. Rinse specimens with Innovex Enhancing Wash Buffer prior to applying primary antibody;
2. Incubate with primary antibody per manufacturer’s instructions;
3. Rinse with Innovex Enhancing Wash Buffer;
4. Incubate with secondary antibody;
5. Rinse with Innovex Enhancing Wash Buffer;
6. Incubate with Enzyme label;
7. Rinse with Innovex Enhancing Wash Buffer;
8. Incubate with substrate/chromogen of choice;
9. Rinse with water;
10. Counterstain and mount.



For ELISA assays
Innovex Enhancing Wash Buffers can be used for washing ELISA plates, however, some non-ionic detergent should be added to the solution.


SIGNAL ENHANCER WASH BUFFERS ARE AVAILABLE IN:



Enhancing Wash Buffer for retrieving
AEC and DAB stains


































Item# SizeShelf lifePrice

NB301S

1 liter

2 yrs

$95



NB301

4 liters

2 yrs

$225


NB301-5C1 liter 
(5X concentrate)
2 yrs$255

    


 






Alkaline Phosphatase-Enhancing Wash Buffer for retrieving
Fast-Red, BCIP/ NBT, Innovex Brown and other substrate
chromogens for alkaline phosphatase enzyme staining

































Item# SizeShelf lifePrice

NB302S

1 liter

2 yrs

$95



NB302

4 liters

2 yrs

$225


NB302-5C1 liter
(5X concentrate)
2 yrs$245

    


 


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INNOVEX BIOSCIENCES Uni-Trieve

 

使用方法: 只需40 30min 或60-70  15min

 


優點:

不須煮沸, 也不用壓力鍋、微波爐加熱

citrate(檸檬酸),因此不需要high pH buffer

適用於所有primary antibody

只需要water dry bath

 background free

適用於任何smearanimalhuman組織,

  ex brain, bone,marrow,etc

 



UNI-TRIEVE is a Mild -temperature Universal Retrieval Solution that retrieves  for all antibodies, all human and all animal tissues at only 65-70 degrees celsius in 30 minutes using a simple water bath.

UNI-TRIEVE ; is Totally Background -Free and unlike citrate buffe, High pH buffers, pressure cookers or microwaves, no  background or tissue damage or loss is observed with Uni-Trieve.

UNI-TRIEVE standardizes retrieval, it retrieves all tissues with a single solution.

UNI-TRIEVE's gentle temperature makes it an absolute must for retrieving for immunofluorescence, delicate tissues such as brain, bone marrow and animal tissues.

UNI-TRIEVE is Background-free and it is the retrieval of choice for Immunofluorescence (IF).

OVER-NIGHT Retrieval is now possible with UNI-TRIEVE: simply immerse sides in 40-45 degrees celcius Uni-Trieve using a water bath as the heat source. Next morning  remove slides from the water bath , rinse and proceed with IHC or IF staining.

UNI-TRIEVE USER FRIENDLY PROTOCOL:




UNI-TRIEVE Unique Features


  • Water bath Retrieval. NO microwaves. NO pressure cookers
  • 30 minutes at 65-70C Water bath
  • OVER-NIGHT retrieval at 40-45 degrees celsius
  • pH-independent .One single solution retrieves all tissues for all antibodies.
  • UNI-TRIEVE retrieves all animal and human tissues for all antibodies
  • Total Retrieval time of 30 minutes at 65-70C for all membrane and cytoplasmic antibodies including Cytokeratins
  • Total retrieval time of  time of 45 minutes at 75-80 degrees celcius  for nuclear antigen such as ki-67, ER, PR , etc.
  • A must for cell line preps and delicate tissues such as brain , bone marrow and animal tissues.
  • Background-Free
  • Retrieval of choice for Immunofluorescence (IF)
  • Can be used three times before needing to be discarded.




Uni-Trieve




Order Uni-Trieve Online!
























Item#

Product Description

Unit

Price US $


Buy

NB325

Uni-Trieve

1 Liter


$ 275



NB325-200S

Uni-Trieve-Trial Size

200ml


$ 55






IHC原理:是指在抗體上結合螢光或可呈色的化學物質,利用免疫學原理中抗原和抗體間專一性的結合反應,檢測細胞或組織中是否有目標抗原的存在。  此方式不只可以用來測知抗原的表現量也可觀察抗原所表現的位置。只要是能夠讓抗體結合的物質,也就是具有抗原性的物質包括蛋白質、核酸、多醣、病原體等都可偵測

 


 

 

IHC protocol:

1Deparaffin-脫臘,65-75  30min ,Xylene 10min, repeat X2

2Dehydration-去除殘留切片上的Xylene

      alcohol100﹪→90﹪→80﹪→70﹪→Distill-WaterPBS

3Optional-去除Endogenous peroxidase 活性,3H2O2 10min

4Antigen recovery(抗原修復)-石臘使Ag遮蔽,復原使Ag充分暴露

      Citrate buffer or EDTABoiling(>95℃)for 10 minutes Cool down

5Block & quench-阻斷內源性的biotin作用,serum 10min

6Primary Ab-1hr or O/N

7Secondary Ab-1hr

8Conjugate- (1)Peroxidase will catalyze the substrate           10min

                        (2)convert the chromogen to a color deposit

9Substrate (DAB or AEC)- 5-10 min

10Counterstain-5min

11Dehydration-避免濃度劇烈的改變,導致組織內涵物的流失,造成樣本的傷害

        alcohol75﹪→85﹪→95﹪→100﹪→100﹪→Xylene

12Mounting-mounting medium5min

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H&E Mount


HE染色/封片劑
No alcohol or xylene clearing pre-steps required


         


   


         太鼎生物科技有限公司 (02) 8660-9496 www.biopioneer.com.tw 


FOR PERMANENT MOUNTING OF H&E STAINS FROM WATER OR ALCOHOL


“H&E Mount” is a unique, permanent mounting media designed for coverslipping H&E stains from water or alcohol.
“H&E Mount” hardens quickly and mounted slides are ready for microscopic examination in 10 minutes.
H&E stained slides mounted with “H&E Mount” do not display any loss of eosin or hematoxylin staining that is often observed when H&E slides are mounted from xylene. Therefore, when mounting with “H&E Mount”, less number of dips
(5-10) in eosin is recommended.




產品特點與介紹ADVANCED FEATURES
• Mount directly from water or alcohol
• No dehydration or xylene clearing steps required prior to mounting
• Alcohol differentiation of eosin may be performed prior to mounting
• Permanent
• No fading of eosin or hematoxylin
• User friendly
• Environmentally friendly
• Makes bench top mounting possible (no alcohol or xylene is involved )
• Fluid at room temperature, no warming required
• Sets in 10 minutes, ready for microscopic examination
• Excellent optical clarity and resolution
• Excellent for photography
• Simple soaking in water removes coverslip
• Saves time, mounting time of less than 10 seconds per slide
• Cost effective, eliminates the cost of purchase or discard of xylene
• Available in 125 ml size
• Economical


























Item# Size# of SlidesShelf lifePrice

NB315

125 ml

2000

3 yrs

          
     
   

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Fc receptors是一種glycoproteins, 存在於血液中B細胞、單核細胞、及顆粒細胞。與抗體中Fc region具高親和力,造成非專一性及背景值形成 去除所有非專一性及背景值的問題 針對血液、骨髓或淋巴組織最適用!



 


www.innovexbiosciences.com


Fc Receptor Blocker


 



innovex biosciences公司Fc Receptor blocker【台灣總代理-太鼎生物科技有限公司】 02-86609496


 



 


 


 


 


 


 


 


 


 


 


 


 


 


ADVANCED and UNIQUE FEATURES:
• 30 minute incubation step at room temperature
• Blocks Fc Receptors in both human and animal cells
• Eliminates false positive staining of white blood cells, lymphoid tissues, cytosmears (blood & bone marrow)
• Insures accurate lymphoma and leukemia typing
• Eliminates negative control staining of lymphomas, lymphoid tissues, and blood and bone marrow smears
• Eliminates false positive staining for Kappa and Lambda staining
• Eliminates false positive staining of Reed Sternberg cells
• A must for CD markers staining in immunohistochemistry, immunofluorescence
• A must for CD phenotyping via flow cytometry
• A must for Immunoglobulins (Igs) staining via immunohistochemistry, flow cytometry and immunofluorescence
• A must for kappa and lambda staining


 


Innovex Fc Receptor Blocker does not contain any antibodies, immunoglobulins or immunoglobulin fragments 

Now also available in azide-free for live cell/functional assays
Azide-Free Fc Blocker can be used on live cells and for functional assays without cytotoxicity
Ordering info, scroll down



Innovex Fc Receptor Block is a peerless technology designed to block Fc Receptors present on all leukocytes (white blood cells), lymphomas, and melanomas. Fc receptors are also expressed on a majority of tumors. Blocking Fc receptors is essential for accurate typing of lymphoid and tumor tissues and cells. Fc receptor staining occurs by the binding of Fc receptors present on cell to the Fc region of the primary and/or secondary antibody. Fc Receptor Block can be used for IHC (frozen and paraffin) flow cytometry, immunoflorescence and in-situ hybridization and for live cell functional assays.



Fc Receptor Blocker is a must for accurate lymphoma and leukemia typing.



ADVANCED and UNIQUE FEATURES:
• 30 minute incubation step at room temperature
• Blocks Fc Receptors in both human and animal cells
• Eliminates false positive staining of white blood cells, lymphoid tissues, cytosmears (blood & bone marrow)
• Insures accurate lymphoma and leukemia typing
• Eliminates negative control staining of lymphomas, lymphoid tissues, and blood and bone marrow smears
• Eliminates false positive staining for Kappa and Lambda staining
• Eliminates false positive staining of Reed Sternberg cells
• A must for CD markers staining in immunohistochemistry, immunofluorescence
• A must for CD phenotyping via flow cytometry
• A must for Immunoglobulins (Igs) staining via immunohistochemistry, flow cytometry and immunofluorescence
• A must for kappa and lambda staining




 


Please click this icon to download the data sheet in PDF file.


 








































Item# Size# of SlidesShelf life
Buy

NB309

60 ml

700-1000

3 yrs


NB309-15

15 ml

150 - 250

3 yrs



NB335 (NEW)

30 ml

Azide - Free

1 yr

     

 



 


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【如何移除免疫組織染色的背景值】


 


在做mouse組織切片時,很容易發生backgroud的問題。


尤其是冷凍切片的組織。Innovex Fc Receptor Block跟您的背景值說再見!!


特別應用於IHCIFIn situ hybridation實驗。



 

Innovex Fc Receptor Block】 引先最新科技設計,針對白血球 leukocytes (white blood cells),淋色瘤 lymphomas, 及黑色素瘤 melanomas部份進行專一性阻斷。


 


 


組織內大量存在Fc receptorsFc receptors是一種glycoproteins, 存在於血液中B細胞、單核細胞、顆粒細胞 dendritic細胞。組織內Fc receptors與抗體中Fc region具高親和力,FcRs-mediated Ig Fc binding 造成非專一性及背景值形成。Fc receptor也是大量存在大部份的腫瘤細胞組織中,因此在免疫組織染色IHC (frozen and paraffin)、流氏細胞分析 flow cytometry, 免疫螢光染色 immunoflorescence 及原位雜合反應試 in-situ hybridization,會使用Fc receptor Blocker 除去抗體Fc region所有非專一性及背景值的問題,尤其是處理血液、骨髓或淋巴的組織!!!


 


 Fc receptor Blocker 先在組織切片中作用15~30分鐘,之後加入一抗或二抗,就不會造成抗體中的Fc region與組織Fc receptor反應。


 


Fc receptors are also expressed on a majority of tumors. Blocking Fc receptors is essential for accurate typing of lymphoid and tumor tissues and cells. Fc receptor staining occurs by the binding of Fc receptors present on cell to the Fc region of the primary and/or secondary antibody. Fc Receptor Block can be used for IHC (frozen and paraffin) flow cytometry, immunoflorescence and in-situ hybridization and for live cell functional assays.


 


IHC過程中,若是使用biotinavidin的系統, 建議使用biotin/avidinblocking buffer,在抗體反應之前,先移去內源性biotin/avidin。如此就不會有非一性binding的問題。


 


【產品特點與規格】



• 30 minute incubation step at room temperature
• Blocks Fc Receptors in both human and animal cells
• Eliminates false positive staining of white blood cells, lymphoid tissues, cytosmears (blood & bone marrow)
• Insures accurate lymphoma and leukemia typing
• Eliminates negative control staining of lymphomas, lymphoid tissues, and blood and bone marrow smears
• Eliminates false positive staining for Kappa and Lambda staining
• Eliminates false positive staining of Reed Sternberg cells
• A must for CD markers staining in immunohistochemistry, immunofluorescence
• A must for CD phenotyping via flow cytometry
• A must for Immunoglobulins (Igs) staining via immunohistochemistry, flow cytometry and immunofluorescence
• A must for kappa and lambda staining


 

 





























Item#



Size



# of Slides



Shelf life



NB309



60 ml



700-1000



3 yrs



NB309-15



15 ml



150 - 250



3 yrs



NB335 (NEW)



30 ml



Azide - Free



1 yr



 



 



 



 



 











 
 










 


關鍵字:








 Fc Blocker, FcγR blocker, Fc inhibitor
Keywords:  (Fc Receptor Blocking Solution), Solution, Fc Blocker, FcγR blocker, Fc inhibitor , Immunology, Antibodies








Other Names: Fc Blocker, FcγR blocker, Fc inhibitor
更多參考內容:

原廠連結: http://www.innovexbiosciences.com/


 


WWW.BIOPIONEER.COM.TW


太鼎生物科技有限公司(02)86609496










  
 










  
 








Other Names: Fc Blocker, FcγR blocker, Fc inhibitor
Keywords:  (Fc Receptor Blocking Solution), Solution, Fc Blocker, FcγR blocker, Fc inhibitor , Immunology, Antibodies


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【如何降低免疫組織染色過程中背景值 第一部份】


IHC過程中,若是使用biotinavidin的系統, 建議使用biotin/avidinblocking buffer在抗體反應之前,先移去內源性biotin/avidin。如此就不會有非一性binding的問題。原理:阻斷內源性biotin (rich in liver,kidney,spleen) 所導致背景值的問題 適用於任何humananimal組織 。


【如何降低免疫組織染色過程中背景值 第二部份】


一般一抗或二抗都有Fc region 組織內含有大量Fc receptorFc region會和Fc receptor結合,


所以造成組織切片會很髒, 尤其冷凍切片的組織Fc receptor更多。建議在抗體反應之前,先將Fc receptor blocker, 如此當加入抗體後,便不會和fc region結合。

 適用於任何humananimal組織


去除不必要的背景,呈現組織真正面貌



適用In-situ HybridizationIHC

Mouse-On-MouseImmunofluorescence

不需要考量HOST來源


使用方法:只要在一抗前加入2~3滴即可


Fc RECEPTOR BLOCK
Peptide Based Technology


N O _M O R E _Fc Receptor Staining
N O
_M O R E  background


Innovex Fc Receptor Blocker does not contain any antibodies, immunoglobulins or immunoglobulin fragments 

Now also available in azide-free for live cell/functional assays
Fc Blocker can be used on live cells and for functional assays without cytotoxicity
Ordering info, scroll down

























Negative control lymph node paraffin section with Fc receptor staining



Same section treated with Innovex Fc receptor block









Negative control mouse tissue with 
Fc receptor staining



Same section treated with Innovex Fc receptor block



Innovex Fc Receptor Block is a peerless technology designed to block Fc Receptors present on all leukocytes (white blood cells), lymphomas, and melanomas. Fc receptors are also expressed on a majority of tumors. Blocking Fc receptors is essential for accurate typing of lymphoid and tumor tissues and cells. Fc receptor staining occurs by the binding of Fc receptors present on cell to the Fc region of the primary and/or secondary antibody. Fc Receptor Block can be used for IHC (frozen and paraffin) flow cytometry, immunoflorescence and in-situ hybridization and for live cell functional assays.



Fc Receptor Blocker is a must for accurate lymphoma and leukemia typing.



ADVANCED and UNIQUE FEATURES:
• 30 minute incubation step at room temperature
• Blocks Fc Receptors in both human and animal cells
• Eliminates false positive staining of white blood cells, lymphoid tissues, cytosmears (blood & bone marrow)
• Insures accurate lymphoma and leukemia typing
• Eliminates negative control staining of lymphomas, lymphoid tissues, and blood and bone marrow smears
• Eliminates false positive staining for Kappa and Lambda staining
• Eliminates false positive staining of Reed Sternberg cells
• A must for CD markers staining in immunohistochemistry, immunofluorescence
• A must for CD phenotyping via flow cytometry
• A must for Immunoglobulins (Igs) staining via immunohistochemistry, flow cytometry and immunofluorescence
• A must for kappa and lambda staining


 


                                                          BACKGROUND BUSTER


N O _M O R E _B A C K G R O U N D


Please click this icon to download the data sheet in PDF file.



  • Mouse-On-Mouse
  • Human and Animal IHC
  • Immunofluorescence
  • in-situ Hybridization
  • Flow Cytometry

Background Buster is highly effective for quenching background fluorescence.

Easy to use:



  • Apply Background Buster for 15 minutes prior to application of the first antibody (unlabeled or fluorescence-labeled antibody).
  • Rinse with PBS for 1 minute.
  • Apply unlabeled antibody for indirect method or fluorescence labeled antibody for direct method and continue with usual protocol.

Please click this icon to download the data sheet in PDF file.


 






























Item#



Size



# of Slides



Shelf life



NB309



60 ml



700-1000



3 yrs



NB309-15



15 ml



150 - 250



3 yrs



NB335 (NEW)



30 ml



Azide - Free



1 yr



 



 



 



 






Background Buster can be used for IHC:





Background or non-specific staining is often observed in a variety of immunoassays, in immunohistochemistry and other immunoassay types such as immunofluorescence, ELISA and flow cytometric assays, background staining can be prevented by the use of INNOVEX Recombinant Protein Technology, Background Buster.



Innovex
Background Buster is a diverse and powerful RECOMBINANT PROTEIN TECHNOLOGY for eliminating general background staining in tissues and cell preparations stained with antibodies in immunohistochemistry or with DNA or RNA probes for in-situ procedures. It also makes possible the staining of mouse antibodies on mouse tissues.



Background Buster is also applicable to eliminating non-specific binding in immunofluorescence, ELISA and flow cytometric assays.



ADVANCED & UNIQUE FEATURES:

• Allows staining of identical species antibodies and tissues (e.g., mouse antibody on mouse tissue, rat-on-rat, rabbit-on-rabbit, etc.).
• Short 10 minute incubation step prior to applying primary antibody or in-situ probe at room temperature
• Delivers complete eradication of general background staining
• Replaces the use of normal serum, powdered milk, casein, and other blocking agents and renders complete success
• Excellent for both frozen and paraffin sections
• Excellent for
in situ application
• A must for animal tissue staining


 
























Item#



Size



# of Slides



Shelf life



NB306



125 ml



1400- 2000



3 yrs



NB306-50



50 ml



700 - 1000



3 yrs



 



 



 



 



更多參考內容:



原廠連結: http://www.innvx.com/



 

WWW.BIOPIONEER.COM.TW


太鼎生物科技有限公司(02)86609496


 

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Total Proteins


 


分離自約200個不同成人與胎兒的正常組織、人類疾病或腫瘤組織,以及小鼠、大鼠、猴子與植物組織。研究不同組織的蛋白質表現時,總蛋白質是應用最廣泛的產品。



產品特色


組織總蛋白質由完整組織經均質化而得,經 SDS-PAGE 驗證具有相同的成分。


總蛋白質保存於具有綜合蛋白脢抑制劑的緩衝溶液中,其濃度為 5 m g/ml


保存於 -70 oC 具有0.1mg/ 0.5m g/ 1m g包裝



應用範圍


電泳、西方墨點轉印、免疫沉澱
酵素活性分析


蛋白質分子間作用力分析


特定組織之基因表現研究


 


Total Protein Arrays


 


取自具文件紀錄之組織或細胞株,組織經切除後立即冷凍,進行病理鑑定。
利用 CytoMol 專屬技術分離出總蛋白質,並點於特殊硝化纖維薄膜上。
GAPDH 抗體進行免疫分析以確保品質與蛋白量一致。
本產品可立即使用,不需耗費時間與精力於自行尋找組織與取得所需蛋白樣本


 


產品特色


可使用微陣列掃瞄器或一般自動放射顯像法偵測


蛋白質預點,可立即使用


可配合組織陣列與 mRNA 陣列使用


適於放射性與非放射性偵測方式


可選擇隨附組織臨床病史


可選購相同組織之切片或 cDNA 以進行免疫化學、原位雜合或 PCR 偵測


單一陣列內涵括所有類型之成人與胎兒正常組織蛋白,以及人類腫瘤組織蛋白


 


應用範圍


微陣列掃描器的大量篩選或一般照片顯影


針對大範圍之正常成人、胎兒或腫瘤組織進行特定蛋白質之快速篩選


蛋白質表現 pattern 分析


目標蛋白質之表現數量比較


Proteins form human tissues
Adult Tissue
Adult and Fetal Tissues
Fetal Tissue
Tumor Tissue


 


Tissue Arrays


 


Tissue Arrays 組織陣列



將組織點於帶正電之玻片上
每片 slide 包含15種不同動物的正常組織


用以測試抗體的物種專一性
PAR34035 Multiple Species Frozen Tissue Array
Brain, 2 slides
PAR34036 Multiple Species Frozen Tissue Array
Brain, 5 slides
PAR34149 Multiple Species Frozen Tissue Array
Liver, 2 slides
PAR34150 Multiple Species Frozen Tissue Array
Liver, 5 slides


 


Total Protein Western Blots


 


產品特色
組織蛋白經過4-20% gradient SDS-PAGE 後轉印至PVDF membrane
每個 lane loading 一個 tissue
total proteins
內含人類胎盤蛋白作為internal standard 以判別蛋白表現量

帶有10KD-250KD prestained protein marker
本產品可立即使用,不需耗費時間與精力

於自行尋找組織與取得所需蛋白樣本


 


應用範圍


 


Identifying specific protein expression 


Determining protein size


Analyzing protein expression pattern


Comparing expression levels of novel proteins


Examining alternative splicing and premature termi-
nation of a specific protein


Proteins From Human Tissues
Adult Tissue
Fetal Tissue
Tumor Tissue (DD)
Tumor Tissue (SD)
Tumor Cell Lines


Proteins From Non-Human Tissues
Monkey (Cynomolgus)
Monkey (Rhesus)
Mouse
Plant
Rat
Other species


 


Mega Western Protein Arrays


 


total proteins SDS-PAGE 分離後,每個 lane 分為 24 fractions, 點於特殊硝基纖維薄膜
GAPDH 抗體進行免疫分析以確保品質與蛋白質量一致


 


產品特色



品質穩定,結果具再現性
靈敏度更高,可用於放射性與非放射性檢測

操作容易,與一般 western blotting 相同
單一晶片可檢測30個不同組織

晶片大小為 2×1 inch2,節省抗體用量 (為傳統 western blot ¼)


在乾燥條件下可室溫保存。

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