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目前分類:【ELISA 、EIA分析套組】 (18)

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Cloud-Clone corp.公司介紹

 

NP_BIG.jpg

 

Cloud-Clone Corp previously USCN Life Science Inc.

專門生產研究用檢測試劑的生技公司,在美國研發和生產,其產品線包括ELISA KitCLIA KitAntibodyrecombinant proteinELISA產品系列超過6000種以上,物種齊全包括ratmouserabbitcaviahumanporcinebovinesimian等多物種商品。產品通過ISO 9001:2008認證(質量體系認證)和ISO134852003(醫療器械質量體系認證)。部分產品有合格證書(CE)認證。品質保證。ELISA商品的型式多原, 提供三明治ELISA、競爭型ELISA、高靈敏度設計ELISA

 

Cloud-Clone offer a large portfolio of products containing over 6000 ELISA kits, exclusively available in the UK and Ireland through Caltag Medsystems.

Examples of the types of ELISA kits available include:

  • Sandwich ELISA Kits for specific protein isoforms (read more)
  • Double Sandwich ELISA Kits for heterodimer detection (read more)
  • Competitive Inhibition ELISA Kits for detection of small molecules (read more)
  • Instant ELISA for increased efficiency and convenience (read more)

 

Apoptosis細胞凋亡/ELISA Kits 酵素免疫套組產品

Cloud-clone provide excellent customer service and technical support. Cloud-Clone's quality management system is certified with ISO 9001:2008 and ISO 13485:2003 certificates, with all products inspected by a three-level quality control system: including raw-material QC, semi-finished product QC, and end-product QC. A free positive control is also contained in most products.

Cloud-Clone are constantly developing new products, providing the tools to study target molecules related to the latest research fields. ELISA kits are available for the following areas of research. Click on the research area for featured products and full product lists:

Apoptosis

Current most popular products include:

  • ELISA kit for B-Cell Leukaemia/ Lymphoma 2 (Bcl2) SEA778
  • ELISA kit for Caspase 3 SEA626
  • ELISA kit for Caspase 9 SEA627
  • ELISA kit for Caspase 7 SEA449

Cardiovascular Biology心血管/ELISA Kits 酵素免疫套組產品

Cardiovascular Biology

Current most popular products include:

  • ELISA kit for Angiotensin II (AngII) CEA005
  • ELISA Kit for Angiopoietin 1 (ANGPT1) SEA008
  • ELISA Kit for Nitric Oxide Synthase 3, Endothelial (NOS3) SEA868
  • ELISA Kit for Dipeptidyl Peptidase IV (DPP4) SEA884

Cytokines細胞激素/ELISA Kits 酵素免疫套組產品

Cytokines

Current most popular products include:

  • ELISA Kit for Interferon Gamma (IFNg) SEA049
  • ELISA Kit for Beta-Thromboglobulin (bTG) SEA370
  • ELISA Kit for Interleukin 4 (IL4) SEA077
  • ELISA Kit for Interleukin 6 (IL6) SEA079

Infection感染及免疫immunityELISA Kits 酵素免疫套組產品

Infection and Immunity

Current most popular products include:

  • ELISA Kit for High Mobility Group Protein 1 (HMG1) SEA399
  • ELISA Kit for C Reactive Protein (CRP) SEA821
  • ELISA Kit for Complement Component 3a (C3a) SEA387
  • ELISA Kit for Complement Component 5a (C5a) SEA388

METABOLISM代謝/ELISA Kits 酵素免疫套組產品

Metabolism

Current most popular products include:

  • ELISA Kit for Tryptase (TPS) SEB070
  • ELISA Kit for Advanced Glycation End Product (AGE) CEB353
  • ELISA Kit for Apolipoprotein A1 (APOA1) SEA519
  • ELISA Kit for Trypsin (TRY) SEA250

NeuroscienceELISA Kits 酵素免疫套組產品

Neuroscience

Current most popular products include:

  • ELISA Kit for Netrin 1 (Ntn1) SEB827
  • ELISA Kit for S100 Calcium Binding Protein B (S100B) SEA567
  • ELISA Kit for Ubiquitin Carboxyl Terminal Hydrolase L1 (UCHL1) SEG945
  • ELISA Kit for Huntingtin (HTT) SEH922

Signal transduction訊息傳遞/ELISA Kits 酵素免疫套組產品

Signal Transduction

Current most popular products include:

  • ELISA Kit for Cyclophilin B (CYPB) SEA227
  • ELISA Kit for Early Growth Response Protein 1 (EGR1) SEA416
  • ELISA Kit for Angiopoietin 4 (ANGPT4) SEA668

Small MoleculesELISA Kits 酵素免疫套組產品

Small Molecules

Current most popular products include:

  • ELISA Kit for Lipopolysaccharide (LPS) CEB526
  • ELISA Kit for Hyaluronic Acid (HA) CEA182
  • ELISA Kit for Pentosidine (PTD) CEA264
  • ELISA Kit for Glutathione (GSH) CEA294

Tumour ImmunityELISA Kits 酵素免疫套組產品

Current most popular products include:

  • ELISA Kit for Hypoxia Inducible Factor 1 Alpha (HIF1a) SEA798
  • ELISA Kit for Squamous Cell Carcinoma Antigen 1 (SCCA1) SEB372
  • ELISA Kit for Cyclophilin A (CYPA) SEA979
  • ELISA Kit for Mucin 5 Subtype AC (MUC5AC) SEA756

 

Antibodies抗體/Proteins蛋白質/

Antibodies and Proteins

Cloud-Clone also manufacture over 800 CLIA kits, 9000 proteins, and 17000 antibodies (suitable for IHC, WB and ELISA) which target across a wide variety of species including human, mouse, rat, bovine, ovine, porcine and others, with multi-species kits available to detect homologous proteins across multiple species (read more).

The majority of stock antibodies listed above can also be modified with FITC or Biotin labels on request.

The Cloud-Clone Corp. manufacturing process is based on the ability to efficiently separate and purify natural proteins and also to manufacture recombinant proteins in order to raise specific antibodies. Cloud-Clone possesses several patented technologies and intellectual property rights in the field of Protein separation and ELISA production, with four dedicated research and development sites. Their product quality management system is certified to ISO 9001:2008 and ISO 13485:2003 standards, with 85% of their products exported to international markets, including Europe, the USA and Japan.

 

 

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ELISA Kit for Immunoglobulin M (IgM)



































Organism species




Homo sapiens
(Human)




Product No.




SEA543Hu




Sample type




Serum, plasma,
tissue homogenates, cell lysates, cell culture supernates and other
biological fluids.




Format




96-well strip
plate




Assay length




4.5 hours




Detection range




31.25-2000ng/mL
The standard curve concentrations used for the ELISA’s were 2000ng/mL,
1000ng/mL, 500ng/mL, 250ng/mL, 125ng/mL, 62.5ng/mL, 31.25ng/mL




Sensitivity




The minimum
detectable dose of this kit is typically less than 12.7ng/mL.



 





 

 


Specificity





This assay has high sensitivity and excellent
specificity for detection of Immunoglobulin M (IgM).

No significant cross-reactivity or interference between Immunoglobulin M (IgM)
and analogues was observed.





Recovery





Matrices listed below were spiked with certain level
of recombinant Immunoglobulin M (IgM) and the recovery rates were calculated by
comparing the measured value to the expected amount of Immunoglobulin M (IgM)
in samples.



























Matrix




Recovery range (%)




Average(%)




serum(n=5)




91-101




98




EDTA plasma(n=5)




80-101




96




heparin
plasma(n=5)




91-101




94






Precision





Intra-assay Precision (Precision within an assay): 3
samples with low, middle and high level Immunoglobulin M (IgM) were tested 20
times on one plate, respectively.

Inter-assay Precision (Precision between assays): 3 samples with low, middle
and high level Immunoglobulin M (IgM) were tested on 3 different plates, 8
replicates in each plate.

CV(%) = SD/meanX100

Intra-Assay: CV<10%

Inter-Assay: CV<12%





Linearity





The linearity of the kit was assayed by testing
samples spiked with appropriate concentration of Immunoglobulin M (IgM) and
their serial dilutions. The results were demonstrated by the percentage of
calculated concentration to the expected.



































Sample




1:2




1:4




1:8




1:16




serum(n=5)




93-101%




88-95%




90-99%




79-97%




EDTA plasma(n=5)




85-104%




89-98%




81-90%




79-96%




heparin
plasma(n=5)




84-102%




86-93%




96-104%




79-94%






Stability





The stability of kit is determined by the loss rate
of activity. The loss rate of this kit is less than 5% within the expiration
date under appropriate storage condition.

To minimize extra influence on the performance, operation procedures and lab
conditions, especially room temperature, air humidity, incubator temperature
should be strictly controlled. It is also strongly suggested that the whole
assay is performed by the same operator from the beginning to the end.





Reagents and materials provided

















































Reagents




Quantity




Reagents




Quantity




Pre-coated, ready
to use 96-well strip plate




1




Plate sealer for
96 wells




4




Standard




2




Standard Diluent




1×20mL




Detection Reagent
A




1×120µL




Assay Diluent A




1×12mL




Detection Reagent
B




1×120µL




Assay Diluent B




1×12mL




TMB Substrate




1×9mL




Stop Solution




1×6mL




Wash Buffer (30 ×
concentrate)




1×20mL




Instruction
manual




1






Assay procedure summary





1. Prepare all reagents, samples and standards;

2. Add 100µL standard or sample to each well. Incubate 2 hours at 37
oC;

3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37
oC;

4. Aspirate and wash 3 times;

5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37
oC;

6. Aspirate and wash 5 times;

7. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37
oC;

8. Add 50µL Stop Solution. Read at 450nm immediately.





Test principle





The test principle applied in this kit is Sandwich
enzyme immunoassay. The microtiter plate provided in this kit has been
pre-coated with an antibody specific to Immunoglobulin M (IgM). Standards or
samples are then added to the appropriate microtiter plate wells with a
biotin-conjugated antibody specific to Immunoglobulin M (IgM). Next, Avidin conjugated
to Horseradish Peroxidase (HRP) is added to each microplate well and incubated.
After TMB substrate solution is added, only those wells that contain
Immunoglobulin M (IgM), biotin-conjugated antibody and enzyme-conjugated Avidin
will exhibit a change in color. The enzyme-substrate reaction is terminated by
the addition of sulphuric acid solution and the color change is measured
spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of
Immunoglobulin M (IgM) in the samples is then determined by comparing the O.D.
of the samples to the standard curve.





 


 

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ELISA Kit for Immunoglobulin G (IgG)



































Organism species




Homo sapiens
(Human)




Product No.




CEA544Hu




Sample type




Serum, plasma and
other biological fluids.




Format




96-well strip
plate




Assay length




2.5 hours




Detection range




1.23-100ug/mL The
standard curve concentrations used for the ELISA’s were 100ug/mL, 33.33ug/mL,
11.11ug/mL, 3.7ug/mL, 1.23ug/mL




Sensitivity




The minimum
detectable dose of this kit is typically less than 0.48ug/mL.






Specificity





This assay has high sensitivity and excellent
specificity for detection of Immunoglobulin G (IgG).

No significant cross-reactivity or interference between Immunoglobulin G (IgG)
and analogues was observed.





Recovery





Matrices listed below were spiked with certain level
of recombinant Immunoglobulin G (IgG) and the recovery rates were calculated by
comparing the measured value to the expected amount of Immunoglobulin G (IgG)
in samples.



























Matrix




Recovery range (%)




Average(%)




serum(n=5)




80-94




85




EDTA plasma(n=5)




84-92




88




heparin
plasma(n=5)




80-95




86






Precision





Intra-assay Precision (Precision within an assay): 3
samples with low, middle and high level Immunoglobulin G (IgG) were tested 20
times on one plate, respectively.

Inter-assay Precision (Precision between assays): 3 samples with low, middle
and high level Immunoglobulin G (IgG) were tested on 3 different plates, 8
replicates in each plate.

CV(%) = SD/meanX100

Intra-Assay: CV<10%

Inter-Assay: CV<12%





Linearity





The linearity of the kit was assayed by testing
samples spiked with appropriate concentration of Immunoglobulin G (IgG) and
their serial dilutions. The results were demonstrated by the percentage of
calculated concentration to the expected.



































Sample




1:2




1:4




1:8




1:16




serum(n=5)




79-99%




97-105%




80-91%




78-97%




EDTA plasma(n=5)




79-97%




90-104%




87-96%




81-99%




heparin
plasma(n=5)




85-102%




82-96%




78-92%




85-98%






Stability





The stability of kit is determined by the loss rate
of activity. The loss rate of this kit is less than 5% within the expiration
date under appropriate storage condition.

To minimize extra influence on the performance, operation procedures and lab
conditions, especially room temperature, air humidity, incubator temperature
should be strictly controlled. It is also strongly suggested that the whole
assay is performed by the same operator from the beginning to the end.





Reagents and materials provided











































Reagents




Quantity




Reagents




Quantity




Pre-coated, ready
to use 96-well strip plate




1




Plate sealer for
96 wells




4




Standard




2




Standard Diluent




1×20mL




Detection Reagent
A




1×120µL




Assay Diluent A




1×12mL




TMB Substrate




1×9mL




Stop Solution




1×6mL




Wash Buffer (30 ×
concentrate)




1×20mL




Instruction
manual




1






Assay procedure summary





1. Prepare all reagents, samples and standards;

2. Add 50µL standard or sample to each well.

    And then add 50µL prepared Detection Reagent A
immediately.

    Shake and mix. Incubate 1 hour at 37oC;

3. Aspirate and wash 3 times;

4. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37oC;

5. Aspirate and wash 5 times;

6. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37oC;

7. Add 50µL Stop Solution. Read at 450 nm immediately.





Test principle





This assay employs the competitive inhibition enzyme
immunoassay technique. An antibody specific for Immunoglobulin G (IgG) has been
pre-coated onto a microplate. A competitive inhibition reaction is launched
between Horseradish Peroxidase (HRP) labeled Immunoglobulin G (IgG) and
unlabeled Immunoglobulin G (IgG) (Standards or samples) with the pre-coated
antibody specific for Immunoglobulin G (IgG). After incubation the unbound
conjugate is washed off. The amount of bound HRP conjugate is reverse
proportional to the concentration of Immunoglobulin G (IgG) in the sample.
After addition of the substrate solution, the intensity of color developed is
reverse proportional to the concentration of Immunoglobulin G (IgG) in the
sample.




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Interleukin 12 Receptor Beta 2 (IL12Rb2)


IL12R-B2; IL12-RB2; RP11 -102M 16.1




































Catalog
No.




RPB073Hu01




Organism




Homo
sapiens (Human)




Applications




SDS-PAGE;
WB; ELISA; IP.




Certification







Designed
by




Cloud-Clone
Corp.( USA )




Assembled
by




Uscn
Life Science Inc.Wuhan







 




























Source




Prokaryotic
expression




Host




E.coli




Molecular
Mass




11.7kDa




Purity




>
95%




U O
M




10ug
/ 50ug / 100ug




Price




Please consult
local dealer









Residues: Tyr559~Gln647
(Accession # Q99665), with N-terminal His-Tag. The target protein is fused with
N-terminal His-Tag, its sequence is listed below. MGHHHHHHSGSEF-YW KERDSNSQPQ
LCEIPYRVSQ NSHPINSLQP RVTYVLWMTA LTAAGESSHG NEREFCLQGK ANWMAFVAPS ICIAIIMVGI
FSTHYFQ





 















































RPB073Hu01




Interleukin 12 Receptor Beta 2
(IL12Rb2)




 




Homo
sapiens (Human)




11.7kDa




Purity
> 95%




RPB073Mu01




Interleukin 12 Receptor Beta 2
(IL12Rb2)




 




Mus
musculus (Mouse)




19.3kDa




Purity
> 95%




RPB073Mu02




Interleukin 12 Receptor Beta 2
(IL12Rb2)




 




Mus
musculus (Mouse)




19.1kDa




Purity
> 95%







 




 




 




 




 





 





【連絡人】許虹宜 0920-312382 Hung-Yi Hsu



E-mailtaiding.biotech@msa.hinet.net



【公司電話】02-86609496【公司傳真】02-86609342



【相關網址】www.uscnk.com



【公司網址】www.biopioneer.com.tw


 


 





 

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HSPG2 ELISA Kit





[ INTENDED USE ] Manual





The kit is a sandwich enzyme
immunoassay for in vitro quantitative measurement of HSPG2 in human serum,





plasma,
tissue homogenates and other biological fluids.





[ TEST PRINCIPLE ]





The microtiter plate provided
in this kit has been pre-coated with an antibody specific to HSPG2. Standards
or





samples are then added to the
appropriate microtiter plate wells with a biotin-conjugated antibody
preparation





specific for HSPG2. Next,
Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate
well





and incubated. After TMB
substrate solution is added, only those wells that contain HSPG2,
biotin-conjugated





antibody and enzyme-conjugated
Avidin will exhibit a change in color. The enzyme-substrate reaction is





terminated by the addition of
sulphuric acid solution and the color change is measured spectrophotometrically
at





a wavelength of 450nm ± 10nm.
The concentration of HSPG2 in the samples is then determined by comparing





the O.D.
of the samples to the standard curve.





 





[供應廠商】太鼎生物科技有限公司





【連絡人】許虹宜 0920-312382 (Hung-Yi Hsu)





E-mailtaiding.biotech@msa.hinet.net





【臺北公司】02-86609496





【相關網址】www.biopioneer.com.tw




【原廠網址】

www.uscnk.com



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TMB Stop Solution (450nm), Solution





Technical Bulletin


訂購貨號# 0412-01































Cat. No.




0412-01




Applications




ELISA




Format




UNLB




Size




100 mL




Volume




100 mL




Regulatory Status




RUO






 





【供應廠商】太鼎生物科技有限公司





【連絡人】許虹宜 0920-312382 taiding.biotech@msa.hinet.net





【臺北公司】02-86609496
【傳真】02-86609342





【相關網址】www.biopioneer.com.tw




【原廠網址】
www.southernbiotech.com

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TMB One Component Microwell Substrate,
Solution





訂購貨號#0410-01

































Cat. No.




0410-01





Applications




ELISA





Format




UNLB





Size




100 mL





Volume




100


mL



 
























Technical Bulletin






【供應廠商】太鼎生物科技有限公司





【連絡人】許虹宜 0920-312382 taiding.biotech@msa.hinet.net





【臺北公司】02-86609496 【傳真】02-86609342





【相關網址】www.biopioneer.com.tw





【原廠網址】www.southernbiotech.com




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ABTS Substrate, Powder




訂購貨號#0202-01


 


























Cat. No.




0202-01




Applications




ELISA




Format




UNLB




Size




500 mg




Regulatory Status




RUO





 





































Technical
Bulletin









MSDS





 




 




【供應廠商】太鼎生物科技有限公司




【連絡人】許虹宜 0920-312382 taiding.biotech@msa.hinet.net




【臺北公司】02-86609496 【傳真】02-86609342




【相關網址】www.biopioneer.com.tw




【原廠網址】www.southernbiotech.com




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ELISA Kit for Retinol Binding Protein 4, Plasma
(RBP4)





Retinol
Binding Protein 4, Plasma (RBP4)





PDF RBP4 ELISA套組操作手冊下載, 貨號#E90929Hu















測量範圍




9.38-600ng/mL




靈敏度




3.35ng/mL







Retinol binding protein (RBP) 4 is the only specific
transport protein for vitamin A in the circulation whose function is to deliver
vitamin to target tissues. In obesity and type 2 diabetes, expression of Glut4
is significantly impaired in adipocytes. Glucose transport via Glut4 is the
rate-limiting step for glucose use by muscle and adipose tissue.




Yang et al. noted that
adipocytespecific deletion of Gluts led to notable elevation of RBP4 causing
systemic insulin resistance, and that reduction of RBP4 improved insulin
resistance. This identified a novel role of RBP4 in regulating insulin action
and RBP4 is recorded as an adipocytederived hormone. Thus, measurement of serum
or plasma RBP4 is a useful means for understanding of metabolic disorders.


 


【供應廠商】太鼎生物科技有限公司



【連絡人】許虹宜0920-312382



【連絡信箱】taiding.biotech@msa.hinet.net



【臺北公司】02-86609496【台中公司】0935-229803 陳俊豪



【公司網址】 www.biopioneer.com.tw


 

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ELISA Kit for Transthyretin (TTR)





TBPA; PA; PLB;
Prealbumin; PALB; Prealbumin Amyloidosis Type I; Transports Thyroxine and
Retinol





PDF TTR ELISA套組操作手冊下載, 貨號# E90726Hu















測量範圍




93.75-6,000pg/mL




靈敏度




36.55pg/mL






 





TTR was originally called prealbumin because it ran
faster than albumins on electrophoresis gels.Prealbumin is produced by the
choroid plexus, by pancreatic islet cells in the embryonic yolk sac, and by
enterochromaffin cells in the gastrointestinal mucosa, but the liver is
quantitatively the most important source.9 Liver production is maintained until
late in liver disease.

Hydration status does not affect prealbumin levels. A negative acute phase
reactant, the prealbumin level will transiently decrease in the presence of
inflammation and in the immediate postsurgical period. Serum levels also
decline in patients with conditions associated with protein malnutrition, such
as malignancy, cirrhosis, protein-losing enteropathy, and zinc deficiency.






【供應廠商】太鼎生物科技有限公司



【連絡人】許虹宜0920-312382



【連絡信箱】taiding.biotech@msa.hinet.net



【臺北公司】02-86609496【台中公司】0935-229803 陳俊豪



【公司網址】 www.biopioneer.com.tw


 


 

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谷胱甘肽S-轉移酶標籤(GST tagELISA檢測試劑盒

靈敏度高1 ng/ml GST標籤蛋白,快速,約1小時完成檢測


用於鑑定細胞裂解物或者純化後的GST標籤蛋白,檢測蛋白的表達,以及蛋白表達優化過程中的快速測定。該試劑盒也可用於檢測GST標籤在標籤切除和蛋白純化後的殘留情況。





實驗原理




本試劑盒應用固相雙抗體夾心酶聯免疫檢測原理測定樣品GST標籤蛋白的含量。用純化的抗GST標籤的單克隆抗體包被微孔板,製成固相抗體,往包被單抗的微孔中依次加入GST 標籤蛋白樣品和GST 標籤蛋白標準樣,再與Biotin標記的另一個抗GST標籤的單克隆抗體結合。再加入HRP標記的Streptavidin形成抗體-抗原-酶標抗體複合物,經過清洗後加底物TMB顯色。TMBHRP酶的催化下轉化成藍色,並在酸的作用下轉化成最終的黃色。顏色的深淺和樣品中的標籤蛋白的量呈正相關。用酶標儀在450nm波長下測定吸光度(OD值),通過標準曲線計算樣品標籤蛋白濃度。





主要特點




靈敏度:1 ng/mL GST標籤蛋白


線性範圍:3.125~100 ng/mL


檢測樣品:細胞裂解液,純化後的GST標籤蛋白等


操作時間:快速,約1小時完成檢測


操作簡單:僅需要簡單的樣品處理就可以進行檢測


品質穩定:嚴格的生產工藝保證了批次間的穩定性





產品目錄













產品編號




產品名稱




規格




L00411




  谷胱甘肽S-轉移酶標籤(GST tagELISA檢測試劑盒: (GST tag ELISA Detection Kit)




1Kit (96 assay)








【供應廠商】太鼎生物科技有限公司




【連絡人】許虹宜 0920-312382




【臺北公司】02-86609496 【台中公司】0935-229803




【相關網址】 www.biopioneer.com.tw





 


 

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組蛋白去乙醯酶3Histone Deacetylase 3ELISA酵素免疫分析套組


  



HDAC3 Activity Assay Kit
Catalog#: K343-100  | Size: 100 assay.



 


Kit Summary:
• Detection method- Fluorescence (Em/Ex = 380/500 nm) 
• Sample type- Cell and Tissue lysates, plasma and serum, other biological fluids 
• Species reactivity- Mammalian 
• Applications- Suitable for high throughput measurement of HDAC3 activity in purified, immunoprecipitated and recombinant or genetically modified HDAC-3 samples.


Features & Benefits: 
• Simple procedure; takes ~ 60 min 
• Fast and convenient 
• Kit contains the necessary reagents for accurate measurement of HDAC3 activity


Kit Components: 
• HDAC3 Assay Buffer 
• HDAC3 Substrate 
• HDAC3 Positive Control 
• AFC Standard (1 mM) 
• Developer 
• Trichostatin A (10 µM)


Description: 
Histone deacetylases (HDACs) represent a large family of enzymes identified as key regulators of nucleosomal histone acetylation, a major event that controls eukaryotic gene transcription and are classified into three groups. HDACs are transcriptional repressors with crucial roles in mammalian development. They are believed to be involved in important biological activities, such as cell differentiation, proliferation, apoptosis, and senescence. In BioVision’s HDAC-3 activity Kit, HDAC-3 and Developer will deacetylate and cleave the substrate [R-H-K-K(Ac)-AFC] to release the quenched fluorescent group (AFC), which can be detected at Em/Ex = 380/500 nm. The kit provides a rapid, simple, sensitive, and reliable test, which is also suitable for high throughput measurement of HDAC-3 activity in purified, immunoprecipitated and recombinant or genetically modified HDAC3 samples.


Storage Conditions: 
-20°C (Upon receipt place HDAC-3 positive control at -80 °C for storage.)


Shipping Conditions: 
gel pack

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IGF Like Family, Member 2 (IGFL2) ELISA酵素免疫分析套組 (Uscnlife ELISA KIT)台灣代理商


 







IGFL2 belongs to the insulin-like growth factor (IGF) family of signaling molecules that play critical roles in cellular energy metabolism and in growth and development, especially prenatal growth .By EST database analysis and DNA sequence assembly, Emtage et al. (2006) identified 4 members of a human gene family, which they designated IGF-like (IGFL), encoding small secreted proteins.IGFL2 encodes a deduced 95-amino acid protein with a signal peptide followed by a conserved region with 11 regularly spaced cysteine residues, including 2 CC motifs. Real-time PCR detected IGFL 2 in cerebellum, heart, placenta, spleen, stomach, testis, and thymus. Emtage et al. (2006) identified only 1 Igfl sequence in mouse, which cannot be assigned direct orthology with any of the human IGFL







E81616HuELISA Kit for IGF Like Family, Member 2 (IGFL2)





E81616HuELISA Kit for IGF Like Family, Member 2 (IGFL2)























Manual


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肝功能分析試劑


 



 


1.      A型肝炎 (anti-HAV)


   









IT-004-011



Anti-VP1 (HAV)



Antibody



200ug/1mg



 


3.      C型肝炎 (anti-HCV)


 









IT-004 -005M3



Anti-E2(HCV)



Antibody



100ug/500ug



 


4.      B型肝炎抗原 (HBsAg)


 








IT-004-0011 



Anti-HBsAg (Pre-S2 Specific) (HBV)



Antibody



 


5.      B型肝炎抗體 (HBsAb)


 









IT-004-002Ep



PreS2-HBsAg(HBV/Genotype C)



Protein



100ug/1mg



 


6.      肝功能指數  (GOT)


 








 K753-100 



 Aspartate Aminotransferase (AST or SGOT) Activity Assay Kit  



 100 assays 



 


7.      肝功能指數  (GPT)


 







K752-100 



 Alanine Aminotransferase (ALT or SGPT) Activity Assay Kit  



 


8.      酒精性肝功能指數 (r-GTP)


9.      肝癌指標  (AFP)


10.  總蛋白指數  (TP)


11.  白蛋白指數  (Alb)


12.  球蛋白指數  (Glo)


13.  總蛋白指數  (T-Bil)


14.  直接膽紅素  (D-Bil)

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如何克服ELISA問題;


標準曲線值較低而且不同濃度標準品之間沒有梯度。


要判斷原因,如下所示;


1.      試劑盒如何保存?


2.      是否按照說明書的要求,在臨用前配製各種反應試劑,如標準品溶液,檢測液A及檢測液B工作液?


3.      酶催化底物反應多長時間,即從加入TMB到加入終止液的時間


4.      溫育的反應溫度是37度,是使用恒溫箱溫育還是水浴?


5.      酶標儀讀數的波長是否設置於450nm


在我們的網站上有一個ELISA試劑盒的操作視頻,客戶可對照著進行操作,這樣可以避免操作原因引起的原因不可用。網站網址如下http://www.uscnk.cn/news/2010428143735.htm



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Guanosine Triphosphate (GTP) ELISA KIT, E96823















 CAS 86-01-1
 USCN 96823
 Wiki GTP


Guanosine-5'-triphosphate (GTP) is a purine nucleotide. It can act as a substrate for the synthesis of RNA during the transcription process. Its structure is similar to that of the guanine nucleobase, the only difference being that nucleotides like GTP have a ribose sugar and three phosphates, with the nucleobase attached to the 1' and the triphosphate moiety attached to the 5' carbons of the ribose.

It also has the role of a source of energy or an activator of substrates in metabolic reactions, like that of ATP, but more specific. It is used as a source of energy for protein synthesis.GTP is essential to signal transduction, particularly with G-proteins, in second-messenger mechanisms where it is converted to GDP (guanosine diphosphate) through the action of GTPases.

 ELISA Kits(Enzyme-linked immunosorbent assay Kits)



Catalog
Product Name
Organism
Manual




E96823GeELISA Kit for Guanosine Triphosphate (GTP)General  n/a
 CLIA Kits(Chemiluminescent immunoassay Kits)



Catalog
Product Name
Organism
Manual




C96823GeCLIA Kit for Guanosine Triphosphate (GTP)General  n/a

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Mouse IgG  ELISA 套組, 5010-01【Immunoglobulin ELISA Kits免疫球蛋白ELISA套組】96孔盤已CoatingELISA過程大約2個半小時



ELISA for the Quantitative Determination of


Mouse IgG in Serum or Plasma



Mouse IgG ELISA KIT, 5010-1


Mouse IgM ELISA KIT, 5015-1





























































































ELISA Kit



Kit Instructions



Catalog #



Chicken IgG



insert



5010-5



Chicken IgM



insert



5015-5



Dog IgG



insert



5010-4



Dog IgM



insert



5015-4



Monkey IgA



insert



5016-3



Monkey IgG



insert



5010-3



Monkey IgG1



insert



5010-3-1



Monkey IgM



insert



5015-3



Monkey IgE



insert



5017-3



Mouse IgA



insert



5016-1



Mouse IgG



insert



5010-1



Mouse IgG1



insert



5010 -1A



Mouse IgG 2a



insert



5010-1B



Mouse IgG2b



insert



5010 -1C



Mouse IgG3



insert



5010-1D



Mouse IgM



insert



5015-1



Rat IgA



insert



5016-2



Rat IgG



insert



5010-2



Rat IgM



insert



5015-2



Turkey IgG



insert



5010-6



Turkey IgM



insert



5015-6



INTRODUCTION


The mouse IgG ELISA kit is designed for measurement of IgG in mouse serum or plasma. The assay uses goat anti-mouse IgG for solid phase (microtiter wells) immobilization and horseradish peroxidase (HRP) conjugated goat anti-mouse IgG antibodies for detection. Both capture and detection antibodies were crossabsorbed


on mouse IgM and IgA agarose columns, thereby ensuring specificity for IgG. Cross-reactivity with immunoglobulins from other species has not been investigated.


PRINCIPLE OF THE TEST


Test samples are diluted and incubated in the microtiter wells for 45 minutes alongside prepared mouse IgG standards. The microtiter wells are subsequently washed and HRP conjugate is added and


incubated for 45 minutes. IgG molecules are thus sandwiched between the immobilization and detection antibodies. The wells are then washed to remove unbound HRP-labeled antibodies and TMB


Reagent is added and incubated for 20 minutes at room temperature. This results in the development of a blue color. Color development is stopped by the addition of Stop Solution, changing the color to yellow, and optical density is measured spectrophotometrically at 450 nm. The concentration of IgG is proportional to the optical density of the test sample and is derived from a standard curve.


MATERIALS AND COMPONENTS


Materials provided with the kit:


· Anti mouse IgG coated 96-well plate (12 strips of 8 wells)


· HRP Conjugate Reagent, 11 ml


· Reference standard (lyophilized)1


· 20x Wash Solution, 50 ml


· 10x Diluent (50 ml)


· TMB Reagent (One-Step) 11 ml


· Stop Solution (1N HCl), 11 ml


Materials required but not provided:


· Precision pipettes and tips


· Distilled or deionized water


· Polypropylene or glass tubes


· Vortex mixer


· Absorbent paper or paper towels


· Micro-Plate incubator/shaker mixing speed of ~150 rpm


· Plate washer


· Plate reader with an optical density range of 0-4 at 450nm


· Graph paper (PC graphing software is optional)


SAMPLE PREPARATION


General Note: IgG is typically present in mouse serum or plasma at concentrations of ~15 mg/ml. In order to obtain values within range of the standard curve, we suggest that samples initially be diluted 200,000 fold using the following procedure for each sample to be tested:


Page 2


1. Dispense 998 ml and 798 ml of 1x diluent into separate tubes.


2. Pipette and mix 2 ml of the serum/plasma sample into the tube containing 998 ml of diluent. This provides a 500 fold diluted sample.


3. Mix 2 ml of the 500 fold diluted sample with the 798 ml of diluent in the second tube. This provides a 200,000 fold dilution of the sample.


4. Repeat this procedure for each sample to be tested Tissue extracts and body fluids other than serum or plasma will likely have lower IgG levels than those found in serum. Optimal dilutions of such samples should be determined empirically.


ASSAY PROCEDURE


1. Secure the desired number of coated wells in the holder.


2. Dispense 100 ml of standards and diluted samples into the wells (we recommend that samples be tested in duplicate).


3. Incubate on an orbital micro-plate shaker at 100-150 rpm at room temperature (18-25°C) for 45 minutes.


4. Aspirate the contents of the microtiter wells and wash the wells


5 times with 1x wash solution using a plate washer (400 ml/well). The entire wash procedure should be performed as quickly as possible.


5. Strike the wells sharply onto absorbent paper or paper towels


to remove all residual wash buffer.


6. Add 100 ml of enzyme conjugate reagent into each well.


7. Incubate on an orbital micro-plate shaker at 100-150 rpm at room temperature (18-25°C) for 45 minutes.


8. Wash as detailed in 4 to 5 above.


9. Dispense 100 ml of TMB Reagent into each well.


10. Gently mix on an orbital micro-plate shaker at 100-150 rpm at room temperature (18-25°C) for 20 minutes.


11. Stop the reaction by adding 100 ml of Stop Solution to each well.


12. Gently mix. It is important to make sure that all the blue color changes to yellow.


13. Read the optical density at 450 nm with a microtiter plate


reader within 5 minutes.

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