~【NEW!!活動快報】~ 全景玻片影像掃描器,精準對焦與掃描,染色結果從此數位化
MoticEasyScan 全景玻片影像掃描器,3D多層次掃描,快速對焦精準掃描為數位玻片。提供最佳解析度0.25um/pixel ;6片式片夾,walk-away設計。搭配簡易操作軟體,優質影像數位永久保存。歡迎來電洽詢(02)86609496許虹宜

目前分類:【DNA、PCR、QPCR分生試劑】 (29)

瀏覽方式: 標題列表 簡短摘要

新型 安全核酸染劑

 

SafeView™/Safedye是一種可代替溴化乙錠(EtBr)的新核酸染料,其為新一代安全性螢光染劑,靈敏度與EtBr相當。

 

 

核酸觀察箱 (UV/Blue)須知

 

一般核酸觀察箱分為UV與藍光二種系統;傳統的UV Box觀察箱, 具有長波(365nm)、中波(312nm)、短波(256nm)波段激發。長波能量較弱 (365nm) ,適合切膠等膠體回收(gel extraction)用。短波能量強(256nm),可以取得較明顯的影像。新一型Safe Blue藍光觀察箱取代UV的波長為470nm 藍光波段,減少UV光線對樣品及眼睛造成的傷害搭配不同的核酸觀察箱,abmgood提供不同波長之核酸染劑將核酸影像最佳化。也減少做實驗誘癌之風險發生。

----------------------------------

傳統的核酸染劑, EtBr (Biovisioin #1203-10)

溴化乙錠 (縮寫為EtBr)是一種核酸染劑用為瓊脂糖凝膠電泳後,核酸之染色UV紫外光照射下,未結合的ErBr可被激發出橙紅色螢光;結合的DNA或雙股RNA,螢光強度會增強20倍,使得核酸電泳後可分辨核酸相對的分子量。EtBr其激發(Excitation)波段為310nm,散射光(emmision)570nm需注意溴化乙錠 (EtBr)為是一種強的誘變劑intercalator,可致癌或致畸。操作時必須佩戴手套。

-----------------------------

新型安全核酸染劑, SafeView/Safedye

SafeView/Safedye安全染劑,產品經由Ames test測試,証明對生物體無致癌性。產品激發(Excitation)波段為290~490nm,散射光(Emmision)480~605nm不同染劑呈現不同激發(Excitation)及散射光(Emmision)使用方式分為二種型式Safeview(內或外染)safedye(直接當loading dye使用)請選擇合的波長範圍,才能將影像最佳化

SafeViewTM系列產品:Classic/FireRed/Plus (內染或外染)

SafeView™ EtBr替代品 (內染或外染使用)。使用方法與EtBr類似,無須更改使用習慣,可使用藍光/UV偵測。

SafeView™ ClassicdsDNAssDNA可呈現綠色螢光、RNA呈現紅色螢光。
SafeView™ FireReddsDNAssDNA、以及RNA呈現紅色螢光。
SafeView Plus™-作為外染使用。不需退染步驟。靈敏度更佳。

SafedyeTM系列產品:Safe-Green、Red、White、Pack (loading dye染劑)

Safe dyes直接跑電泳時, loading dye使用。是一種快速染核之loading dye不需將膠體預染或外染。

------------------------------

靈敏度比較 (核酸安全染劑 V.S. EtBr)

傳統EtBr染劑,其靈敏度是檢測至少1ng以下的DNA;新型安全染劑,具有更高的signal:Bkgrd的比值,靈敏度高於傳統型EtBr染劑。在選擇不同染劑前,也請選擇適的波長範圍,才能將影像最佳化。

Safe-Green has Excitation Wavelength of 490nm and Emission Wavelength of 525nm, and its sensitivity range is between 0.2-0.6ng.

Safe-Red has Excitation Wavelength of 540nm and Emission Wavelength of 630nm, and its sensitivity range is between 0.3-0.8ng.

Safe-White has Excitation Wavelength of 370nm and Emission Wavelength of 470nm, and its sensitivity range is between 0.2-0.5ng.

SafeView Classic emits green fluorescence when bound to dsDNA and ssDNA and red fluorescence when bound to RNA. This stain has one excitation (490 nm) and two emission spectra (520 nm and 635 nm) and the sensitivity range is between 0.1-0.3ng.

SafeView Plus has Excitation Wavelength of 490nm and Emission Wavelength of 525nm and its sensitivity range is between 0.05-0.1ng.

---------------------------------------

核酸安全染劑產品應用:

可使用在瓊脂糖agarose或丙烯醯胺凝膠polyacrylamide gels中的 dsDNAssDNARNA。搭配不同波長濾片進行DNA偵測。In gel stiningGel extractionLigationTransformationTransfection

---------------------------------------

核酸安全染劑使用方法:

SafeView 產品100ml agarose溶液中加入5ul SafeView™ Classic 後製膠,
Safedye為跑電泳時將maker與樣本中加入SafeView™ dye (1:5倍稀釋)

(內染) SafeView™ ClassicFireRed100ml agarose溶液中加入5μl SafeView™ 後進行製膠。

(外染) SafeView Plus™DNA電泳完成後,將膠體泡入含有SafeView Plus™的電泳緩衝液(1:10,000稀釋),室溫下反應10-40分鐘即可進行偵測。

(loading dye也是DNA染劑) SafeView™ dyes:將maker/樣本中加入SafeView™ dye1:5稀釋)後進行DNA電泳即可。

--------------------------------------

 

safeview_result

-------------------------------------------

產品編號 產品名稱 波長(Ex/Em) 靈敏度(ng) 包裝

SafeView™ Classic-內染

G108 SafeView™ Classic

490nm/ 635nm

490nm/520nm

0.1-0.3 1ml

SafeView Plus™-外染

G468 SafeView Plus™ 290nm/520nm 0.05-0.1 1ml

SafeView™ dyes

G108-G Safe-Green™

290nm/525nm

490nm/525nm

0.2-0.6 1ml
G108-R Safe-Red™ 490nm/630nm 0.3-0.8 1ml
G108-W Safe-White™ 370nm/470nm 0.2-0.5 1ml
G108-P Safe-Pack™

參照G108-G、R、W

----------------------------------------------

HOT!! 其它核酸染劑試劑商品/如下連結

 

DAPI細胞核核酸染劑商品 (Abmgood, #b551)

◆ EtBr核酸染劑商品 (biovision, #1203-10)

PI (Propidium iodime)DNA細胞核染劑 (細胞凋亡/biovision, #1056-1)

 

--------------------------------------

HOT!! 其它核酸染劑儀器商品/如下連結

 

DNA水平電泳槽 (Mupid 2 plus)

UV觀察箱 (Major Science)

safeblue藍光核酸觀察箱 (Major Science)

核酸影像分析系統 (Major Science)

 

 

<更多 相關諮詢請洽>

 

代理廠商】: 太鼎生物科技有限公司

【公司電話】: 02-86609496 傳真 : 02-86609342
【公司網站】: www.biopioneer.com.tw
【連絡業務】: 許虹宜 0920312382
【連絡信箱】: taiding.biotech@msa.hinet.net

 

 

#

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全血直接PCR-----不需萃取DNA





將全血加入2X PCR MasterMix,即可進行PCR反應。血液中大量存在 PCR inhibitors(抑制劑)PCR inhibitors抑制PCR polymerase反應,因為無法直接由全血中進行PCR反應。傳統全血PCR,全血需經由DNA萃取,才能進行PCR反應。Abmgood應用Error-Prone
PCR
技術,研發Bestag DNA Polymerase,克服PCR inhibitors之干擾,客戶僅將血液加入Bloodirect DNA
Polymerase
,即可進行PCR反應。





(PCR反應液需額外購買)





 若需Bloodirect Tag 資料如下所示:





http://tw.myblog.yahoo.com/taiding2000/article?mid=1076&prev=-1&next=1073





 















Cat.No.:




G465           




Quantity:




5ml



 






產品特點





1.    
全血不需萃取DNA之步驟,即可進行PCR反應。




2.    
20%
(v/v)
全血反應體積,不需前處理血液樣品




3.    
血液樣品可來自新鮮血液、冷凍血液、EDTA保存之全血、FTA Elute Card之全血、Guthrie Cards之全血、或flter paper之全血。




4.    
可應用於各哺乳動物之血液PCR,包括鳥類之血液PCR




5.    
降低污染之可能性





 


產品介紹





 





Bestaq DNA
Polymerase
使用Error-Prone PCR技術,效能高,靈敏度佳。Bloodirect 2X PCR Mastermix 包含Bloodirect DNA
Polymerase, dNTPs,
buffer Ready-to-use混合液。.客戶直接使用全血,即可進行PCR反應。排除DNA萃取之需求。全血之使用量,可佔PCR反應體積之20%。在EDTACitrate、及10 % (v/v) heparin以下之存在,皆不受影響PCR之反應。乾血sample (Guthrie cards or 903 cards (Whatman, NJ)),取1 mm punch disc,即可直接PCR反應。





 


 





產品內容物





訂購貨號#G465----- (200個反應,反應體積50ul)





Bloodirect
2X PCR MasterM 5ML








Mg離子濃度建議





Magnesium
Concentration





Final [1X] Bloodirect MasterMix contains 3.0 mM Magnesium Sulfate, total Magnesium
Sulfate concentration of up to 6.0 mM
can be used. If necessary, Magnesium concentration can also be lowered with
EDTA solution to eliminate non-specific amplification.





 


產品資料內容





General Notes





1. Whole blood samples containing sodium citrate, sodium
EDTA, potassium EDTA or sodium heparin are recommended.





2. Blood volume higher than 10 % (v/v) would increase the
difficulties in recovering aqueous supernatant from blood cell debris after
PCR.





3. Dry blood stored on Guthrie cards or 903 cards ( Whatman , NJ )
can be used directly by adding 1 mm
punch disc to PCR reaction





4. For blood stored on FTA cards ( Whatman , NJ ),
first incubate a 1 mm punch
disc in 50 μl of water at 55
oC for 5 minutes, then
discard the water and use the disc directly in PCR reaction.





 


5. Up to 10 % (v/v) DMSO can be used for high-GC targets


 







 





【供應廠商】太鼎生物科技有限公司





【連絡人】許虹宜
0920-312382





【台北公司】02-86609496





【相關網址】 www.biopioneer.com.tw





【原廠網址】http://www.abmgood.com





 


 


 

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 全血直接PCR-----不需萃取DNA





血液中大量存在 PCR inhibitors(抑制劑)PCR inhibitors抑制PCR polymerase反應,因為無法直接由全血中進行PCR反應。傳統全血PCR,全血需經由DNA萃取,才能進行PCR反應。Abmgood應用Error-Prone
PCR
技術,研發Bestag DNA Polymerase,克服PCR inhibitors之干擾,客戶僅將血液加入Bloodirect DNA
Polymerase
,即可進行PCR反應。(PCR反應液需額外購買)





若需Bloodirect
2x MasterMix
資料如下所示:  




http://tw.myblog.yahoo.com/taiding2000/article?mid=1077&prev=-1&next=1076




 












Cat.No.:




G 463            G 462




Quantity:




400 U (400 μl)    100 U (400 μl)







產品特點



1.     全血不需萃取DNA之步驟,即可進行PCR反應。


2.     20% (v/v)全血反應體積,不需前處理血液樣品


3.     血液樣品可來自新鮮血液、冷凍血液、EDTA保存之全血、FTA Elute Card之全血、Guthrie Cards之全血、或flter paper之全血。


4.     可應用於各哺乳動物之血液PCR,包括鳥類之血液PCR


5.     降低污染之可能性






產品介紹





 





Bestaq DNA
Polymerase
使用Error-Prone PCR技術,效能高,靈敏度佳。Bloodirect 2X PCR Mastermix 包含Bloodirect DNA
Polymerase, dNTPs,
buffer Ready-to-use混合液。.客戶直接使用全血,即可進行PCR反應。排除DNA萃取之需求。全血之使用量,可佔PCR反應體積之20%。在EDTACitrate、及10 % (v/v) heparin以下之存在,皆不受影響PCR之反應。乾血sample (Guthrie cards or 903 cards (Whatman, NJ)),取1 mm punch disc,即可直接PCR反應。





 





 


產品內容物





訂購貨號#G462----- (25個反應,反應體積50ul)





Bloodirect DNA Polymerase (1
U/μl)  100 μl  





5X Bloodirect Buffer, with
Mg2+     1 ml   





訂購貨號#G463-----(100個反應,反應體積50ul)





Bloodirect DNA Polymerase (1
U/μl)  400 μl  





5X
Bloodirect Buffer, with Mg2+     2ml  





buffer內容物





Storage
Buffer Components





50 mM Tris-HCl (pH 8.0), 100 mM NaCl, 0.1 mM EDTA, 5
mM DTT, 50 % glycerol and 1 % Triton
®X-100.





Mg離子濃度建議





Magnesium
Concentration





Final [1X] Bloodirect Buffer contains 3.0 mM Magnesium Sulfate, total Magnesium
Sulfate concentration of up to 6.0 mM
can be used. If necessary, Magnesium concentration can also be lowered with
EDTA solution to eliminate non-specific amplification.





產品資料內容





General Notes





1. Whole blood samples containing sodium citrate, sodium
EDTA, potassium EDTA or sodium heparin are recommended.





2. Blood volume higher than 10 % (v/v) would increase the
difficulties in recovering aqueous supernatant from blood cell debris after
PCR.





3. Dry blood stored on Guthrie cards or 903 cards ( Whatman , NJ )
can be used directly by adding 1 mm
punch disc to PCR reaction





4. For blood stored on FTA cards ( Whatman , NJ ),
first incubate a 1 mm punch
disc in 50 μl of water at 55
oC for 5 minutes, then
discard the water and use the disc directly in PCR reaction.





5. Up to 10 % (v/v) DMSO can be used for high-GC targets





 



















Concentration




1 U/μl




Storage




Store all
components at -20°C in a
non-frost-free freezer.




Notes




1. Whole
blood samples containing sodium citrate, sodium EDTA, potassium EDTA, or
sodium heparin are recommended.

2. Blood volume higher than 10 % (v/v) would increase the difficulties in
recovering aqueous supernatant from blood cell debris that remains after PCR.

3. Dry blood stored on Guthrie cards or 903 cards ( Whatman , NJ )
can be used directly by adding 1 mm
punch disc to PCR reaction

4. For blood stored on FTA cards ( Whatman ,
NJ ), first incubate a 1 mm punch disc in 50 μl of water at 55°C for 5 minutes, then
discard the water and use the disc directly in PCR reaction.

6. Up to 10% (v/v) DMSO can be used for high-GC targets.






 







 


【供應廠商】太鼎生物科技有限公司



【連絡人】許虹宜 0920-312382



【台北公司】02-86609496



【相關網址】 www.biopioneer.com.tw



【原廠網址】http://www.abmgood.com







 


 

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qPCR Mastermix






































EvaGreen
qPCR Mastermix




Product Name




Machine




Cat. No.




EvaGreen qPCR MasterMix-ROX




-ABI® 7000, 7300, 7700 and
7900, StepOnePlus™ StepOne™




Mastermix-R




EvaGreen qPCR Mastermix-Low ROX




-ABI® 7500

-Stratagene® Mx3000, Mx3005 and Mx4000




Mastermix-LR




EvaGreen qPCR Mastermix-iCycler




-BioRad® iCycler®, iQ™5
and MyiQ™




Mastermix-iC




EvaGreen
qPCR Mastermix




-BioRad® CFX96

-Roche LightCycler® 480

-MJ Research Opticon™ and Opticon™ 2

-MJ Research Chromo® 4

-Corbett Rotor-gene® 6000,3000

-Eppendorf® Realplex




Mastermix-S







更多qPCR相關產品















































































































One-Step
EvaGreen qRT-PCR Kit




Product Name




Machine




Cat. No.




One-Step
EvaGreen qRT-PCR-ROX




-ABI® 7000, 7300, 7700 and
7900, StepOnePlus™ StepOne™




G471-R




One-Step
EvaGreen qRT-PCR-Low ROX




-ABI® 7500

-Stratagene® Mx3000, Mx3005 and Mx4000




G471-LR




One-Step
EvaGreen qRT-PCR-iCycler




-BioRad® iCycler®, iQ™5
and MyiQ™




G471-iC




One-Step EvaGreen
qRT-PCR




-BioRad® CFX96

-Roche LightCycler® 480

-MJ Research Opticon™ and Opticon™ 2

-MJ Research Chromo® 4

-Corbett Rotor-gene® 6000,3000

-Eppendorf® Realplex




G471-S




TaqProbe
qPCR Mastermix




Product Name




Machine




Cat. No.




TaqProbe
2x qPCR Mastermix-ROX




-ABI® 7000, 7300, 7700 and
7900, StepOnePlus™ StepOne™




Mastermix-P




TaqProbe 2x qPCR Mastermix-Low ROX




-ABI® 7500

-Stratagene® Mx3000, Mx3005 and Mx4000




Mastermix-PL




TaqProbe 2x qPCR Mastermix-iCycler




-BioRad® iCycler®, iQ™5
and MyiQ™




Mastermix-PC




TaqProbe 2x qPCR Mastermix-No Dye




-BioRad® CFX96, Opticon™,
Opticon™ 2, and Chromo® 4

-Roche LightCycler® 480

-Corbett Rotor-gene® 6000,3000

-Eppendorf® Realplex




Mastermix-PS




TaqProbe
2x qPCR Mastermix-Multiplex




Suited for Multiplex
TaqMan assay in the following instrument.

-ABI® 7000,7300,7500,7700,7900, StepOne™, StepOnePlus™

-BioRad® iCycler®, iQ™5, CFX96, Opticon™2, Chromo® 4

-Corbett Rotor-gene® 6000,3000

-Stratagene® Mx3000, Mx4000

-Roche LightCycler® 2.0, 480




Mastermix-PM




KiloGreen
qPCR Mastermix




Product Name




Machine




Cat. No.




KiloGreen 2x qPCR
Mastermix-ROX




-ABI® 7000, 7300, 7700 and
7900, StepOnePlus™ StepOne™




Mastermix-KR




KiloGreen 2x qPCR
Mastermix-Low ROX




-ABI® 7500

-Stratagene® Mx3000, Mx3005 and Mx4000




Mastermix-KL




KiloGreen 2x qPCR
Mastermix-iCycler




-BioRad® iCycler®, iQ™5
and MyiQ™




Mastermix-KC




KiloGreen 2x qPCR Mastermix




-BioRad® CFX96

-Roche LightCycler® 480

-MJ Research Opticon™ and Opticon™ 2

-MJ Research Chromo® 4

-Corbett Rotor-gene® 6000,3000

-Eppendorf® Realplex




Mastermix-KS






 





[供應廠商】太鼎生物科技有限公司





【連絡人】許虹宜 0920-312382





E-mailtaiding.biotech@msa.hinet.net





【臺北公司】02-86609496





【相關網址】www.biopioneer.com.tw





【原廠網址】www.abmgood.com





 


 

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qPCR
Lentivirus Titration(Titer) Kit     



















Cat.No.:




LV900




Quantity:




100
Reactions




 




 




qPCR
 Lentivirus
定量套---100個反應





Description





The Lentivirus Titration Kit

















provides
a fast and simple method for titrating lentivirus. This kit employs a quick RNA
extraction step and determines viral RNA using qRT-PCR. The whole assay could
be completed in only 2 hours. The titer primers supplied in this kit are
designed for all HIV-1 based vectors detection. With the help of the on-line
titer calculator, titration of any lentiviral preparation could be easier than
ever before.





 


qPCR
 Lentivirus
定量套---產品特點--






1.  
no RNA purification -- saves time and eliminates
inaccuracy





2.   ready-to-use reagent mix -- reduces
variability





3.   qRT-PCR in one step -- more sensitive
and accurate than other methods





4.   no NTC amplification -- our titration
kit is the only one on the market that completely eliminates NTC signal due to
our unique primer design. Similar kits from other companies all give NTC
amplification, which compromise accuracy for viral titration





 

【供應廠商】太鼎生物科技有限公司



【連絡人】許虹宜 0920-312382 taiding.biotech@msa.hinet.net



【臺北公司】02-86609496 【傳真】02-86609342



【相關網址】www.biopioneer.com.tw



【原廠網址】www.abmgood.com





 

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2x PCR Taq Mastermix with dye


 















Cat.No.:




G013-dye




Quantity:




5.0 ml (200 PCR reactions)





定量PCR反應液200個反應



































Description




The PCR Taq Mastermix is a ready-to-use mixture of high quality Taq DNA
Polymerase, deoxynucleotides, and reaction buffer in a 2X concentrate. It
contains all the necessary reagents for amplification of DNA. To set up a PCR
reaction, just add DNA template, primers and water. Amplification with this
mix generates PCR products with 3'-A overhangs.




Literature



























Application




PCR amplifications with high reproducibility.




Form




Master mix solution




Protocol









Storage




Store all components at -20 °C in a non-frost-free freezer.




Notes




This product is distributed for laboratory research only.

Caution: Not for diagnostic use .






【供應廠商】太鼎生物科技有限公司



【連絡人】許虹宜 0920-312382 taiding.biotech@msa.hinet.net



【臺北公司】02-86609496 【傳真】02-86609342



【相關網址】www.biopioneer.com.tw



【原廠網址】www.abmgood.com


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高效能Competent cell勝任細胞





具備有高效能的Competent cell勝任細胞,是cloning技術基本要領。專利技術讓Competent cell揚名全世界。其高效能的能力,讓cloning更容易,在一分鐘就做完Transformation。依據其應用及菌種細分如下。





NEW!! 超高效能DH5a勝任細胞 –cloning    


 


Competent
cell
勝任細胞 (strain: DH5a)效能>1X10-10 CFU/ug


全世界最常見也是最普遍的strain,
其適用於
大片段的plasmid
DNA Cloning
,其plasmid DNA片段可大於10~40Kb





訂貨編號: (可進行80Cloning) 效能>1X10-10 CFU/ug


產品規格: 100ul/vial, 80vials,


產品附贈: control plasmid 5ul
(10-4ug/ul) pUC19




快速JM109----勝任細胞





Competent cell勝任細胞 (strain: JM109)




配合TA cloing kit, 或是快速ligation kit, 6分鐘完成transformation




cloning變得更容易





HOT!! DH5a----常用型勝任細胞---cloning    


 


Competent
cell
勝任細胞 (strain: DH5a)效能>1X10-8 CFU/ug


全世界最常見也是最普遍的strain,
其適用於大片段的plasmid DNA Cloning,其plasmid DNA片段可大於10~40Kb





訂貨編號 (可進行80Cloning)


產品規格: 100ul/vial, 80vials,


產品附贈: control plasmid 5ul
(10-4ug/ul) pUC19





蛋白質表現BL21----勝任細胞


 


C ompetent cell勝任細胞 (strain: BL21) ; 效能>2X10-7 CFU/ug





BL21適用於生產大量的Protein蛋白質表現BL21,這個菌株主要是帶有一個lac promoter,在promoter之後則有T7 RNA polymerase的基因,整個白質表達還需要質體上帶有T7
promoter
,使用時加入IPTG用以啟動lacpromoter使T7 RNA polymerase產生,之後就可以啟動質體上的T7 promoter。這個菌株對於protein表達比較好的原因在於,因為一般的菌表達過量的蛋白質之後,形成stress,甚至protein本身對菌株就有毒性,這些原因會造成protein產量下降,可是使用DE3系統,可以控制在最佳生長狀況下,進行誘導protein產生,以提高protein的產量與效率,最高可以得到在總蛋白質中,具有50%的產量。





訂貨編號:  (可進行40Cloning)


產品規格: 100ul/vial, 40vials,


產品附贈: control plasmid 5ul
(10-4ug/ul) pUC19





Competent
cell
勝任細胞注意事項】





1.本建議濃度以新鮮配製之抗生素為準。


2.使用過期之抗生素,將因藥性不足,易產生假性轉形菌落。


3.若使用過量的抗生素濃度或同時使用多種抗生素進行篩選,轉形效率會明顯下降。請務必依建議濃度進行抗生素篩選





【轉形之正對照組操作步驟】





每組皆附5 μl
10-4 μg/μl pUC19
作為正對照組之轉形質體。


請將pUC19質體濃度稀釋為10-6-10-7μg/μl,取1 μl 10-6-10-7μg/μl pUC19


進行之轉形,並以含20
μg/ml Ampicillin
LB培養基進行篩選。





【轉形效率之計算】





轉形效率指每1 μg質體DNA經由轉形後,勝任細胞形成的菌落數。例:


10-6 μg pUC19經由轉形後,勝任細胞形成的100個菌落,則轉形效率為100/10-6 =1x108 (cfu/μg)





【藍/白篩選】


 


進行轉形前,請先確認質體帶有LacZ operon。轉形後,請將勝任細胞塗佈於含0.5 mM IPTG 40~60 μg/ml X-galLB培養基。37oC培養後,白色菌落表示外源DNA嵌入LacZ operon,藍色菌落則仍具備正常的β-galactosidase代謝能力。





【含抗生素之LB培養基配方】





10 g/L Tryptone 5 g/L Yeast extract 10 g/L NaCl 15 g/L
Agar
NaOH調整酸鹼值至pH7.5



高溫高壓滅菌後,待降溫至55oC




加入適當濃度之抗生素(見背面說明)





cloning 相關產品:
cloning plating beads
100g


 


【供應廠商】太鼎生物科技有限公司



【連絡人】許虹宜 0920-312382



【台北公司】02-86609496 【台中公司】0935-229803



【相關網址】 www.biopioneer.com.tw



 




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             Flex-C Cloning Kit


           20分鐘如何完成cloning至表現載體




  (不需使用Restriction EnzymePhosphatase T4 ligase)





如何將DNA選殖至目標表現載體上, 不需Restriction enzyme phosphatase ligase? Vivantis最新技術-應用Flex-C酵素, 簡單地將PCR的片段, 置換至具有同源DNA的表現載體上。僅在PCR引子合成中, 設計具有表現載體上的同源序列。PCR反應後, 加入線性DNAFlex-C酵素, 20分鐘後即置換至表現載體上。





Flex-C 產品特點 Features:








1.       
不需Restriction enzyme phosphatase ligase





2.       
選殖任何片段至任何表現載體位置上。





3.       
一次可以clone多種片段





4. 
選殖的片段可以> 10kb





5. 
適用於 5’ overhangs, 3’ overhangs blunt ends





6. 
選殖效能> 95% positive clones





7. 
Adaptor
加入,基因合成、標誌tag加入、linker加入等全面位應用





8.  20分鐘完成cloning





9.  內附PCR反應液 (包括DNA Polymerase酵素、DntpBuffer)









套組內不包括之試劑;(請洽當區sales)





Primer合成;Gene-specific primers with 10bp
homolog to vector ends





表現載體; Linearised
vector





勝任細胞; Competent
cells





微生物培養基; LB plates
containing appropriate antibiotic


 





【供應廠商】太鼎生物科技有限公司



【連絡人】許虹宜 0920-312382



【台北公司】02-86609496



【相關網址】 www.biopioneer.com.tw


 


 


【原廠網址】http://www.vivantechnologies.com

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架構好之mouse cDNA Clone, 具有完整之序列報告。每一個cDNA經由最佳化序列設計改良, 在大部份的細胞株中, 具有最大量之蛋白質表現

 

 

 

 

 

【供應廠商】太鼎生物科技有限公司

【連絡人】許虹宜 0920-312382

【台北公司】02-86609496

【相關網址】 www.biopioneer.com.tw

 

【原廠網址】http://www.sinobiological.com

 

 

 

 

 


Molecule Type Species(Antigen) Description App. Cat & Size/Price

 

 

 

 
IL17RB cDNA Mouse Mouse IL17RB Gene cDNA Clone / ORF Clone   MG51109-G    
 
ESM1 cDNA Mouse Mouse ESM1 Gene cDNA Clone / ORF Clone   MG51108-G    
 
IL27 cDNA Mouse Mouse IL27 Gene cDNA Clone / ORF Clone   MG51107-G    
 
VDR cDNA Mouse Mouse VDR Gene cDNA Clone / ORF Clone   MG51106-G    
 
BAZ1B cDNA Mouse Mouse BAZ1B Gene cDNA Clone / ORF Clone   MG51105-G    
 
LRWD1 cDNA Mouse Mouse LRWD1 Gene cDNA Clone / ORF Clone   MG51104-G    
 
TrkA cDNA Mouse Mouse NTRK1 Gene cDNA Clone / ORF Clone   MG51103-G    
 
Lamb1 cDNA Mouse Mouse Lamb1 Gene cDNA Clone / ORF Clone   MG51102-G    
 
Fgf8 cDNA Mouse Mouse Fgf8 Gene cDNA Clone / ORF Clone   MG51101-G    
 
Sonic Hedgehog cDNA Mouse Mouse SHH Gene cDNA Clone / ORF Clone   MG51100-G    
 
EPO cDNA Mouse Mouse EPO Gene cDNA Clone / ORF Clone   MG51099-G    
 
LY96 cDNA Mouse Mouse LY96 Gene cDNA Clone / ORF Clone   MG51098-G    
 
IL17F cDNA Mouse Mouse IL17F Gene cDNA Clone / ORF Clone   MG51097-G    
 
ErbB3/HER3 cDNA Mouse Mouse ERBB3 partial cDNA Clone / ORF Clone   MG51003-C    
 
CNTN5 cDNA Mouse Mouse CNTN5 Gene cDNA Clone / ORF Clone   MG51092-G    
 
IL1R2 cDNA Mouse Mouse IL1R2 Gene cDNA Clone / ORF Clone   MG51090-G    
 
FCAMR cDNA Mouse Mouse FCAMR Gene cDNA Clone / ORF Clone   MG51089-G    
 
MST4 cDNA Mouse Mouse MST4 Gene cDNA Clone / ORF Clone   MG51088-G    
 
Tie2 cDNA Mouse Mouse TEK Gene cDNA Clone / ORF Clone   MG51087-G    
 
IL23A cDNA Mouse Mouse IL23a Gene cDNA Clone / ORF Clone   MG51086-G    
 
IL22 cDNA Mouse Mouse IL22 Gene cDNA Clone / ORF Clone   MG51085-G    
 
IL4 cDNA Mouse Mouse IL4 Gene cDNA Clone / ORF Clone   MG51084-G    
 
SRPK2 cDNA Mouse Mouse SRPK2 Gene cDNA Clone / ORF Clone   MG51083-G    
 
MBIP cDNA Mouse Mouse MBIP Gene cDNA Clone / ORF Clone   MG51082-G    
 
ROBO4 cDNA Mouse Mouse ROBO4 Gene cDNA Clone / ORF Clone   MG51081-G    
 
PRKD3 cDNA Mouse Mouse PRKD3 Gene cDNA Clone / ORF Clone   MG51080-G    
 
Furin cDNA Mouse Mouse FURIN Gene cDNA Clone / ORF Clone   MG51079-G    
 
RSPO2 cDNA Mouse Mouse Rspo2 Gene cDNA Clone / ORF Clone   MG51078-G    
 
MAPK10 cDNA Mouse Mouse MAPK10 Gene cDNA Clone / ORF Clone   MG51077-G    
 
OCRL cDNA Mouse Mouse OCRL Gene cDNA Clone / ORF Clone   MG51076-G    
 
GP1BB cDNA Mouse Mouse GP1bb Gene cDNA Clone / ORF Clone   MG51075-G    
 
FLRT2 cDNA Mouse Mouse FLRT2 Gene cDNA Clone / ORF Clone   MG51074-G    
 
OBCAM/OPCML cDNA Mouse Mouse OPCML Gene cDNA Clone / ORF Clone   MG51073-G    
 
BTLA cDNA Mouse Mouse BTLA Gene cDNA Clone / ORF Clone   MG51060-G    
 
EGFR/ErbB1/HER1 cDNA Mouse Mouse EGFR Gene cDNA Clone / ORF Clone   MG51091-G    
 
CD34 cDNA Mouse Mouse CD34 Gene cDNA Clone / ORF Clone   MG50589-M    
 
ICOS cDNA Mouse Mouse ICOS Gene cDNA Clone / ORF Clone   MG50466-M    
 
IL1F8 cDNA Mouse Mouse IL1F8 Gene cDNA Clone / ORF Clone   MG51071-G    
 
IL31 cDNA Mouse Mouse IL31 Gene cDNA Clone / ORF Clone   MG51070-G    
 
RIPK3 cDNA Mouse Mouse RIPK3 Gene cDNA Clone / ORF Clone   MG51069-G    
 
IL5 cDNA Mouse Mouse IL5 Gene cDNA Clone / ORF Clone   MG51068-G    
 
IL24 cDNA Mouse Mouse IL24 Gene cDNA Clone / ORF Clone   MG51067-G    
 
IL17A cDNA Mouse Mouse IL17A Gene cDNA Clone / ORF Clone   MG51065-G    
 
TUBB3 cDNA Mouse Mouse TUBB3 Gene cDNA Clone / ORF Clone   MG51063-G    
 
INSR/CD220 cDNA Mouse Mouse INSR Gene cDNA Clone / ORF Clone   MG51062-G    
 
ADAM10 cDNA Mouse Mouse ADAM10 Gene cDNA Clone / ORF Clone   MG51072-G    
 
IL2 cDNA Mouse Mouse IL2 Gene cDNA Clone / ORF Clone   MG51061-G    
 
EBI3 cDNA Mouse Mouse EBI3 Gene cDNA Clone / ORF Clone   MG51058-G    
 
CD163 cDNA Mouse Mouse CD163 Gene cDNA Clone / ORF Clone   MG51057-G    
 
B4GALT1 cDNA Mouse Mouse B4GALT1 Gene cDNA Clone / ORF Clone   MG51056-G    
 
CPE cDNA Mouse Mouse CPE Gene cDNA Clone / ORF Clone   MG51055-G    
 
RNASEL/RNS4 cDNA Mouse Mouse RNASEL Gene cDNA Clone / ORF Clone   MG51054-G    
 
STRADB cDNA Mouse Mouse STRADB Gene cDNA Clone / ORF Clone   MG51053-G  -  
 
PRMT6 cDNA Mouse Mouse PRMT6 Gene cDNA Clone / ORF Clone   MG51052-G    
 
RIPK1 cDNA Mouse Mouse RIPK1 Gene cDNA Clone / ORF Clone   MG51051-G    
 
ETRNL cDNA Mouse Mouse ETRNL Gene cDNA Clone / ORF Clone   MG51050-G    
 
CREG cDNA Mouse Mouse CREG Gene cDNA Clone / ORF Clone   MG51049-G    
 
GM-CSF/CSF2 cDNA Mouse Mouse CSF2 Gene cDNA Clone / ORF Clone   MG51048-G    
 
SDC2 cDNA Mouse Mouse SDC2 Gene cDNA Clone / ORF Clone   MG51047-G    
 
MOG cDNA Mouse Mouse MOG Gene cDNA Clone / ORF Clone   MG51046-G  

 

更多產品如下:

 

 

http://www.sinobiological.com/IL17RB-cDNA-Clone-g-9402.html

 

 

 

 

 

 

 

 

 

 

 

 

 

文章標籤

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Safe-White™    業務專員許虹宜0920-312382


 



 




Cat.No.:G108-W
Quantity:1.0ml


ApplicationSafe Detection of dsDNA, ssDNA and RNA in agarose gels.
ReactionSafe-Green,Red,White,Pack™ - Substitute for loading dye
With SafeView™; dyes (Safe-Green,Red,White,Pack™), you do not need to add any dyes to both gel matrix and running buffers. SafeView™ dyes are provided in a form of 6x sample loading dyes and they are to be added to your samples only. The Safeview™ dyes completely eliminate any possible contamination of glassware or gel running tank as associated
with EB. After the electrophoresis, view and document your results as you would do with EB staining protocols.
Protocol
1. Prepare a 100ml agarose solution.
2. Mix gently without having any air bubbles.
3. Let the solution cool down to 60-70°C and cast the gel.
4. Mix samples and DNA markers with a SafeView™ dye at 1:5 dilution rate.
5. View the results under UV light after electrophoresis.
Image
Protocol
NotesDispose Safe-White™ as you would any other non-carcinogenic fluorescent dye (eg. Acridine orange; Propidium iodide).

This product is distributed for laboratory research only.
Caution: Not for diagnostic use .

 


 

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隨機突變Random Mutagenesis (Stratagene)


Stratagene-reagent



GeneMorph II Random Mutagenesis Kit利用Mutazyme II DNA Polymerase的酵素特性,複製DNA能得到均衡的ATCG 突變比例,產物可達6kb,在GeneMorph II Kit中,可以藉由調整適當的反應條件(MgCl2或者dNTP)來控制突變發生的比例。

randomMT

GeneMorph II EZClone Domain Mutagenesis Kit包含GeneMorph II Random Mutagenesis Kit的ATCG均衡突變比例,另外包含domain swapping的cloning技術,可以方便快速地將突變目標基因接入plasmid中。

ezclone


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多點突變Multi Site-Directed Mutagenesis (Stratagene)


stratagene_reagent



QuikChange Multi Site-Directed Mutagenesis Kits和QuikChange Site-Directed Mutagenesis Kits不同的是,此kit可以一個步驟同時產生多點突變,相較過去單點突變方法,,可同時突變五個不同位點,大幅節省實驗時間及錯誤。快速有效地產生突變點,保有序列複製高準確性,同時獲得高產量產物。

SDMmulti


另有最新的Lightning版本,將點突變技術帶入下一個層次,最適化的高效整合型fusion polymerase讓整個點突變時間可以大為縮減,和QuikChange Multi試劑相比,能夠減少至少一半以上的實驗所需時間。

lightMulti


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分生專用培養基,方便您進行DNA Cloning、Yeast培養。
老品牌培養基LAB M,英國原裝進口,口碑好,其品質與DIFCO相當。採用鋁質密封封口膜,不易受潮,不會有摻其它原料之疑慮。品質保證、無潮解之風險。


 1. 微生物培養基 (粉末培養基, 500g )
2. 各式選擇性培養基 (粉末培養基, 500g )
3. 食品品管培養基 (粉末培養基, 500g )
4. 分子生物實培養基 (粉末培養基, 500g )
5. 各式培養基添加物 (粉末培養基, 500g )


http://www.labm.com


 The introduction of HarlequinTM chromogenic media, the company´s development of ISO compliant media formulations for food industry testing, and the provision of services such as the SMART QA Proficiency Testing scheme have all confirmed Lab M´s leading position.


Lab M also supplies bulk peptones, extracts and other raw materials to pharmaceutical and large-scale fermentation industries, in compliance with current EDQM certification. Custom-designed and custom-blended products are also a speciality.


Lab M is certified in accordance with ISO 9001:2000 and ISO 13485; products for the clinical market are supplied in compliance with the European IVD directive and carry the CE mark.



太鼎生物科技有限公司 (02)86609496


www.biopioneer.com.tw


原廠網站: www.labm.com

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組織/血液/植物/酵母菌/尾巴/細胞/只須95C反應10分鐘,


完成genomic DNA extraction萃取,進行PCR反應。



 


 

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SYBR Real-time PCR Premix, 5ml包裝/5000元


附有Hotstart DNA tag polymerase


 


 


應用實例


 

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 DNA Marker系列 (100bp1Kb DNA 標準液產品)



           


 全面提供低價供應實驗室跑電泳看過來

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快速、高效能、精確的T&A cloning系統如何呈現?



T&A Cloning kit套組內容】


1.    T&A Cloning Vector 20個反應濃度為25ng/ul體積為40ul


2. control insert DNA                 10ng/ul體積為10ul


3. YEA T4 DNA Ligase                  2U/ul 體積為20ul


4. 10x Ligation buffer A                      體積為50ul


5. 10x Ligation buffer B                      體積為50UL


6. M13-F引子                         10uM體積為50ul


7. M13-R引子                         10uM體積為50ul


 



Blunt end ligation如何最佳化?





【Rapid ligation kit套組內容】


1.    T4 DNA Ligase                  2U/ul    體積為100ul


4. 10x Ligation buffer A                      體積為200ul


5. 10x Ligation buffer B                      體積為200ul


 



T&A Vector相關資料


the map of TA Cloning vector



 

T&A Vector限制酶切除位置


Restriction emzyme sites of T&A Cloning vector




T&A Vector實驗結果呈現


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高效能Competent cell勝任細胞


具備有高效能的Competent cell勝任細胞,是cloning技術基本要領。ECOS專利技術讓Competent cell揚名全世界。其高效能的能力,讓cloning更容易,在一分鐘就做完Transformation。依據其應用及菌種細分如下。




上圖一為ECOS 9 -5C ompetent cell勝任細胞 (strain: JM109)

配合TA cloing kit, 或是快速ligation kit, 6分鐘完成transformation


讓cloning變得更容易


 



下圖二為ECOS 101 C ompetent cell勝任細胞 (strain: DH5a)全世界最常見也是最普遍的strain, 其適用於大片段的plasmid DNA Cloning或Yeast two hybrid system,其plasmid DNA片段可大於10~40Kb。



下圖三為ECOS 21 C ompetent cell勝任細胞 (strain: BL21) BL21適用於生產大量的Protein蛋白質表現。BL21,這個菌株主要是帶有一個lac promoter,在promoter之後則有T7 RNA polymerase的基因,整個白質表達還需要質體上帶有T7 promoter,使用時加入IPTG用以啟動lacpromoter使T7 RNA polymerase產生,之後就可以啟動質體上的T7 promoter。這個菌株對於protein表達比較好的原因在於,因為一般的菌表達過量的蛋白質之後,形成stress,甚至protein本身對菌株就有毒性,這些原因會造成protein產量下降,可是使用DE3系統,可以控制在最佳生長狀況下,進行誘導protein產生,以提高protein的產量與效率,最高可以得到在總蛋白質中,具有50%的產量。



【一分鐘完成Transformation示意圖】


 




ECOS產品系列Competent cell勝任細胞注意事項】


1.本建議濃度以新鮮配製之抗生素為準。


2.使用過期之抗生素,將因藥性不足,易產生假性轉形菌落。


3.若使用過量的抗生素濃度或同時使用多種抗生素進行篩選,轉形效率會明顯下降。請務必依建議濃度進行抗生素篩選


ECOSTM轉形之正對照組操作步驟】


每組ECOSTM皆附5 μl 10-4 μg/μl pUC19作為正對照組之轉形質體。


請將pUC19質體濃度稀釋為10-6-10-7μg/μl,取1 μl 10-6-10-7μg/μl pUC19


進行ECOSTM之轉形,並以含20 μg/ml AmpicillinLB培養基進行篩選。


【轉形效率之計算】


轉形效率指每1 μg質體DNA經由轉形後,勝任細胞形成的菌落數。例:


10-6 μg pUC19經由轉形後,勝任細胞形成的100個菌落,則轉形效率為100/10-6 =1x108 (cfu/μg)


【藍/白篩選】


進行ECOSTM轉形前,請先確認質體帶有LacZ operon。轉形後,請將ECOSTM勝任細胞塗佈於含0.5 mM IPTG 40~60 μg/ml X-galLB培養基。37oC培養後,白色菌落表示外源DNA嵌入LacZ operon,藍色菌落則仍具備正常的β-galactosidase代謝能力。


【含抗生素之LB培養基配方】


10 g/L Tryptone 5 g/L Yeast extract 10 g/L NaCl 15 g/L Agar NaOH調整酸鹼值至pH7.5


高溫高壓滅菌後,待降溫至55oC


加入適當濃度之抗生素(見背面說明)


 

【cloning 相關產品: cloning plating beads】100g



 

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。[內文提供不同DNA安全性染劑G108, G108-W, G108-G, G108-R的螢光激發及散射光]讓您的影像擷取更清楚。


SafeView™是一種可代替溴化乙錠(EtBr)的新核酸染料,其為新一代安全性螢光染劑,靈敏度與EtBr相當



一般UV Box觀察箱, 具有長波(365nm)、中波(312nm)、短波(256nm)波段激發。長波能量較弱,適合切膠等膠體回收(gel extraction)用。短波能量強,可以取得較明顯的影像。


EtBr染劑,其激發(Excitation)波段為310nm,散射光(emmision)為570nm。


SafeView安全染劑,其激發(Excitation)波段為290~300nm,散射光(emmision)為605nm。


 


產品介紹:


可使用在瓊脂糖或丙烯醯胺凝膠中的 dsDNAssDNARNA
本產品經由Ames test測試,証明對生物體無致癌性,可安心使用。


In gel stiningGel extractionLigationTransformationTransfection


使用方法:


SafeView 產品100ml agarose溶液中加入5ul SafeView™ Classic 後製膠,
Safedye
為跑電泳時將maker與樣本中加入SafeView™ dye (1:5倍稀釋)



G108-W Safe-White™




Cat.No.:G108-W

 

SafeView™ DNA Stains


   SafeView™ products represent a new and safe class of nucleic acid stains for the visualization of double-stranded DNA, single-stranded DNA, and RNA in agarose gels; a great alternative to more expensive SYBR® Safe or toxic Ethidium Bromide from other competitors. The dyes are developed to replace toxic Ethidium Bromide (EB, a potent mutagen), commonly used in gel electrophoresis for visualization of nucleic acids in agarose gels. SafeView™ products are non-carcinogenic by the Ames-test. The results are negative in both the mouse marrow chromophilous erythrocyte micronucleus and mouse spermary spermatocyte chromosomal aberration tests.

 

Dispose Safe-White™ as you would any other non-carcinogenic fluorescent dye (eg. Acridine orange; Propidium iodide). This product is distributed for laboratory research only. Caution: Not for diagnostic use .

 



Products List



Safe-Green™ G108-G


Safe-Green has Excitation Wavelength of 290nm and Emission Wavelength of 490nm, and its sensitivity is range between 0.2-0.6ng


Safe-Red™ G108-R


Safe-Red has Excitation Wavelength of 490nm and Emission Wavelength of 630nm, and its sensitivity is range between 0.3-0.8ng


 


Safe-Red™ G108-W
Safe-White has Excitation Wavelength of
320nm and Emission Wavelength of 480nm, and its sensitivity is range between 0.2-0.5ng



SafeView™ Classic G108


SafeView Classic has Excitation Wavelength of 290nm and Emission Wavelength of 605nm and the its sensitivity is range between 0.1-0.3ng


 


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