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MoticEasyScan 全景玻片影像掃描器,3D多層次掃描,快速對焦精準掃描為數位玻片。提供最佳解析度0.25um/pixel ;6片式片夾,walk-away設計。搭配簡易操作軟體,優質影像數位永久保存。歡迎來電洽詢(02)86609496許虹宜

目前分類:【間質幹細胞、不杇、初代細胞】 (10)

瀏覽方式: 標題列表 簡短摘要

 




PCR
Mycoplasma Detection Kit    



















Cat.No.:




G238




Quantity:




100
Reactions




 




 



鰴漿菌PCR偵測套組---100個反應





產品內容





Mycoplasma detection is often difficult
due to its lack of visable appearance, therefore it can be afflicting your
valuable cell and affecting your results without your knowledge. Cells
contaminated by mycoplasma species can have changes in proliferation, metabolism,
gene synthesis and processing, and even adhersion properties. The solution for
quick and easy mycoplasma detection is ABM's PCR Mycoplasma Detection Kit.

The PCR Mycoplasma Detection Kit allows for fast and reliable identification of
mycoplasma contamination in cell cultures. Mycoplasma DNA in the cell culture
supernatant is amplified via PCR and visualized using gel electrophoresis. In
addition to the short detection process (less than 2 hours), the easy handling
and high sensitivity makes this PCR Mycoplasma Detection Kit a convenient tool
for routine examination of cell cultures and media.



























產品特點





-direct addition of cell culture
supernatant to PCR reaction — no DNA isolation/purification steps required.

-ready-to-use primer mix — reduces variability.

-able to detect numerous mycoplasma species – high sensitivity.

-included positive control to verify negative results.

-rapid results in 2 hours.





Data Sheet





Product Brochure






Detectable
Mycoplasma Species/Strains List






Notes





Mycoplasma contamination detection and
mycoplasma elimination customer service are also provided, please refer to the Mycoplasma Detection and Elimination Service page
for details





【供應廠商】太鼎生物科技有限公司



【連絡人】許虹宜 0920-312382 taiding.biotech@msa.hinet.net



【臺北公司】02-86609496 【傳真】02-86609342



【相關網址】www.biopioneer.com.tw



【原廠網址】www.abmgood.com



 


 

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Primary Liver Cells 





Abmgood提供一系列不同組織之初代細胞 (primary cells)。克服 primary cells較一般細胞株 (cell line) 取得不易,繼代培養代數有限之問題。提供1x10^6細胞株含量,可進行即時細胞影像攝影、細胞遷移、傷口癒合、共同培養等多樣化的實驗設計與需求。





 

























































































































































Primary
Human Cells




Product




Size




Cat. No.




Price



























Human Primary
Cryopreserved Non-Parenchymal Cells




1x106
cells/1.0ml




T4061




$625.00







Human Primary Total
Liver Cell Population




1x106
cells/1.0ml




T4062




$625.00







Human Primary Hepatic
Progenitor Cells




1x106
cells/1.0ml




T4063




$625.00







Human Primary Fetal
Liver Stromal Cells




1x106
cells/1.0ml




T4064




$625.00







Human Primary Fetal
Liver-CD133+ Cells




1x106
cells/1.0ml




T4065




$625.00







Human Primary Fetal
Liver-CD34+ Cells




1x106
cells/1.0ml




T4066




$625.00







Human Primary
Hepatocytes




1x106
cells/1.0ml




T0051




Inquire







ECM Coated
Flasks for Primary Liver Cells




Product




Size




Cat. No.




Price









T25 Flasks




10 Flasks




G299




$50.00







Cell Culture
Products for Primary Liver Cells




Product




Size




Cat. No.




Price









Prigrow I Medium




500ml




TM001




$95.00







Prigrow II
Medium




500ml




TM002




$95.00







Prigrow III
Medium




500ml




TM003




$95.00







Prigrow IV
Medium




500ml




TM004




$95.00







Trypsin-EDTA




100ml




TM051




$15.00







Trypsin-EDTA




500ml




TM050




$25.00







USDA
Research Grade Origin Fetal Bovine Serum




100ml




TM999-100




$55.00







USDA
Research Grade Origin Fetal Bovine Serum




500ml




TM999-500




$155.00







 


 


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8-Br-cAMP誘導細胞分化

Catalog#:
1837-25 | Size: 25 mg





8--環腺苷酸(8-bromo-cyclic 3
5 -adenosine monophosphat8-Br-cAMP)可選擇性結合cAMP受體位點,誘導細胞分化





Description: A cell-permeable cAMP analog that is more resistant to
hydrolysis by phosphodiesterases than cAMP. Activates protein kinase A,
inhibits growth, decreases proliferation, increases differentiation, and
induces apoptosis of cultured cells. Also recently shown to improve the
reprogramming efficiency of human neonatal foreskin fibroblast (HFF1) cells
2-fold.





Alternate Name/Synonyms: 8- Bromoadenosine- 3', 5'- cyclic monophosphate; cyclic 8-
bromoadenosine- 3', 5'- monophosphate





Appearance: White to off-white solid





CAS Number: 76939-46-3





Molecular Formula: C₁₀H₁₀BrNOP. Na





Molecular Weight: 430.08





Purity: 98%
by TLC





Solubility: HO
(~100 mg/ml)





Storage Temp.: -20°C





Shipping Conditions: gel pack





Handling: Protect from moisture and light





USAGE: For Research Use Only! Not For
Use in Humans





【供應廠商】太鼎生物科技有限公司



【連絡人】許虹宜 0920-312382



【台北公司】02-86609496 【台中公司】0935-229803



【相關網址】 www.biopioneer.com.tw




【原廠網址】http://www.biovision.com


 


 

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Differentiation幹細胞分化誘導劑



----Biovision提供超過280種幹細胞研究抗體


----140種以上Cytokines及Growth factors。


-----90種以上幹細胞命運調控因子


-----StemBoost reprogramming cocktail sets


 






 

 


 


 


























































































































































Catalog #
 


Product Name+




Size




1926-5




Cardiogenol C
hydrochloride




5 mg




1926-25




Cardiogenol C
hydrochloride




25 mg




1688-5




Compound 1




5 mg




1578-5




Cyclopamine




5 mg




1522-100




Cyclosporine A




100 mg




1522-1G




Cyclosporine A




1 g




1855-5




DAPT




5 mg




1042-10G




Dexamethasone




10 g




1042-1G




Dexamethasone




1 g




2092-5




EZSolution™
LDN193189
























5 mg




1746-5




EZSolution™
Rapamycin




5 mg




1872-1




EZSolution™
SB-431542




1 mg




1531-5




Forskolin




5 mg




1812-5




GANT58




5 mg




1812-25




GANT58




25 mg




1892-5




GANT61




5 mg




1890-5




GDC-0449




5 mg




1890-25




GDC-0449




25 mg




1564-1




Geldanamycin




1 mg




1564-5




Geldanamycin




5 mg




1659-1




Hh Signaling
Pathway Antagonist




1 mg




1726-1




JK 184




1 mg




2066-5




Kartogenin





5 mg




2066-25




Kartogenin





25 mg




1904-1




Kenpaullone




1 mg




1995-5




LDN193189





5 mg




1995-25




LDN193189





25 mg




1852-5




MoTP




5 mg




1852-25




MoTP




25 mg




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Differentiation幹細胞分化誘導劑


 



































































































































































Catalog #




Product Name+




Size




1926-5




Cardiogenol C
hydrochloride




5 mg




1926-25




Cardiogenol C
hydrochloride




25 mg




1688-5




Compound 1




5 mg




1578-5




Cyclopamine




5 mg




1522-100




Cyclosporine A




100 mg




1522-1G




Cyclosporine A




1 g




1855-5




DAPT




5 mg




1042-10G




Dexamethasone




10 g




1042-1G




Dexamethasone




1 g




2092-5




EZSolution™
LDN193189
























5 mg




1746-5




EZSolution™
Rapamycin




5 mg




1872-1




EZSolution™
SB-431542




1 mg




1531-5




Forskolin




5 mg




1812-5




GANT58




5 mg




1812-25




GANT58




25 mg




1892-5




GANT61




5 mg




1890-5




GDC-0449




5 mg




1890-25




GDC-0449




25 mg




1564-1




Geldanamycin




1 mg




1564-5




Geldanamycin




5 mg




1659-1




Hh Signaling
Pathway Antagonist




1 mg




1726-1




JK 184




1 mg




2066-5




Kartogenin





5 mg




2066-25




Kartogenin





25 mg




1904-1




Kenpaullone




1 mg




1995-5




LDN193189





5 mg




1995-25




LDN193189





25 mg




1852-5




MoTP




5 mg




1852-25




MoTP




25 mg




1903-5




Neuropathiazol




5 mg



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DNA修復基因沉默細胞株(Knockdown cell lines),全新上市。


 


 


在BER路徑中,提供20種以上基因沉默細胞株。包括鹼基切除修復(BER)的途徑、錯配修復(MMR),和非同源末端連接(NHEJ)。特定shRNA慢病毒轉導之LN428膠質母細胞瘤,經由RT-PCR其基因沉默效能為63%~98%。其無特定病毒之污染,品質保證。


 

Tools to Study Genomic Instability and Genotoxic Stress
DNA Repair Gene Knockdown Cell Lines*

DNA repair proteins maintain the stability of the genome. When repair protein function is impaired through mutations, the genome may become unstable which is a hallmark of solid tumors. The availability of this panel of knockdown cell lines will permit scientists to study the molecular etiology of tumor genomic instability and to exploit it in oncology research. The Knockdown line encompasses the Base Excision Repair pathway, Non Homologous End Joining, and Mismatch Repair pathways.


Trevigen’s Knockdown Cell Lines are target specific LN428 (glioblastoma) shRNA lentivirus transduced cells. They are rigorously qualified and mycoplasma free. The percent knockdown levels range from 63-98% as evaluated by RT-PCR. Lentiviruses are maintained by puromycin selection.



FEATURES:

  • Tested negative for Mycoplasma
  • Tested negative for: Human immunodeficiency virus (HIV1, HIV2), Hepatitis viruses (A, B,and C), Human T-lymphotropic virus (HTLV 1, HTLV 2), Epstein Barr, Hantaviruses Hantaan (Seoul, Sin, Nombre), Herpes simplex (1+2,) Human cytomegalovirus, Human herpes virus (6+8), Human adenovirus, Varicella virus and Lymphocytic Choriomeningitis virus.

ORDERING INFORMATION:

  • *Cell lines are provided under an MTA.



 


Base Excision Repair

Catalog #Product Name# of Cells Per VialSize%KD by RT-PCR
5500-001-01PARP1 Knock Down Cell Line (MTA Required prior to shipment)1x1061 vial72%
5502-001-01BRCA1 Knock Down Cell Line (MTA Required prior to shipment)1x1061 vial83%
5504-001-01OGG1 Knock Down Cell Line (MTA Required prior to shipment)1x1061 vial63%
5505-001-01NTHL1 Knock Down Cell Line (MTA Required prior to shipment)1x1061 vial91%
5507-001-01NEIL2 Knock Down Cell Line (MTA Required prior to shipment)1x1061 vial86%
5508-001-01NEIL3 Knock Down Cell Line (MTA Required prior to shipment)1x1061 vial95%
5510-001-01SMUG1 Knock Down Cell Line (MTA Required prior to shipment)1x1061 vial63%
5511-001-01MPG Knock Down Cell Line (MTA Required prior to shipment)1x1061 vial98%
5512-001-01MutYH Knock Down Cell Line (MTA Required prior to shipment)1x1061 vial87%
5513-001-01Neil1 Knock Down Cell Line (MTA Required prior to shipment)1x1061 vial92%
5514-001-01PARP2 Knock Down Cell Line (MTA Required prior to shipment)1x1061 vial83%
5515-001-01PARP3 Knock Down Cell Line (MTA Required prior to shipment)1x1061 vial70%
5516-001-01XRCC1 Knock Down Cell Line (MTA Required prior to shipment)1x1061 vial81%
5517-001-01APE1 Knock Down Cell Line (MTA Required prior to shipment)1x1061 vial90%
5518-001-01APE2 Knock Down Cell Line (MTA Required prior to shipment)1x1061 vial80%
5519-001-01TDG Knock Down Cell Line (MTA Required prior to shipment)1x1061 vial74%
5506-001-01MBD4 Knock Down Cell Line (MTA required before shipment)1x101 vial72


 


Mismatch Repair

Catalog #Product Name# of Cells Per VialSize%KD by RT-PCR
55027-001-01PMS2 Knock Down Cell Line (MTA Required prior to shipment)1x1061 vial84%
55028-001-01MLH3 Knock Down Cell Line (MTA required before shipment)1x1061 vial71%
55021-001-01MSH3 Knock Down Cell Line (MTA required before shipment)1x1061 vial83%
55034-001-01XRCC6 (Ku70) Knock Down Cell Line (MTA required before shipment)1x1061 vial92%
55026-001-01MLH1 Knock Down Cell Lines (MTA required to shipment)1x1061 vial80%
55024-001-01MSH5 Knock Down Cell Line (MTA required to shipment)1x1061 vial92%
55035-001-01XRCC5 (Ku80) Knock Down Cell Line (MTA required before shipment)1x1061 vial85%
55022-001-01MSH6 Knock Down Cell Line (MTA required to shipment)1x1061 vial93%


 


Non-Homologous End Joining

Catalog #Product Name# of Cells Per VialSize%KD by RT-PCR
55037-001-01SIRTI Knock Down Cell Line (MTA required prior to shipment)1x1061 vial95%
55036-001-01PRKDC Knock Down Cell Line (MTA required prior to shipping)1x1061 vial94%
55038-001-01SIRTI2 Knock Down Cell Line (MTA required prior to shipping)1x1061 vial98%


 


Knockdown Control

Catalog #Product Name# of Cells Per VialSize%KD by RT-PCR
5503-001-01Knock Down Control Cell Line (MTA Required prior to shipment)1x1061 vialN/A




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Abmgood重組
hTERT,p53,Myc,Rb,Ras V12, SV40 T antigen
lentivirus vector, 應用lentivirus的系統, 誘導細胞immortalizing.




哺乳動物細胞使用幾種方法誘導細胞不杇化 (Cell immortalizing)。其中的一個方法,是使用猿猴病毒40SV40T抗原病毒的基因,誘導細胞不杇化。SV40T抗原誘導不同類型的細胞不杇化,在許多文獻中己證實,此方法為最簡單且最可靠的方法,其機制一直都有詳細記載。在大多數情況下,一些抑癌基因 (病毒基因失活),例如P53RB等,可誘導細胞複製衰老狀態實現細胞不杇。最近的研究顯示,SV40T抗原可誘導感染細胞的端粒酶活性。細胞不杇化的方法,是通過端粒酶逆轉錄酶蛋白,特別是對細胞端粒長度(如人類)的影響是最的表達。這種蛋白是無效,但在大多數體細胞端粒酶是外生表現時,能夠保持足夠的端粒長度,以避免複製衰老細胞。幾個端粒酶永生細胞株的分析已證實,細胞維持一個穩定的基因型和保留關鍵的表型標記。 在某些細胞株,尤其是在基層上皮細胞,hTERT的過度表現未能誘導細胞永生化,反而可能會誘導細胞死亡。最近的研究發現,端粒酶催化亞基共表達P53RBsiRNA可以使人類細胞,初代細胞,卵巢細胞,及上皮細胞不杤化(楊 G.等。癌變 2007; G.等,癌基因2007)。同樣,超過 T58A突變的RasMyc的表達也被發現可以不朽一些主要的細胞類型(西爾斯 R.等,基因與開發,2000年)。 Abmgood 憑藉多年在細胞永生化的經驗,開發細胞不杇化的最全面的產品,產品包括端粒酶,P53RBsiRNAsSV40 T抗原架構的質體和慢病毒及腺病毒表現載體。Abmgood提供已定量好的慢病毒和腺病毒,可直接感染細胞,其細胞immortalizing Abmgood慢病毒載體都來源於莫洛尼鼠白血病病毒(MoMuLV)。







Abmgood提供定量好的病毒, 藉由lentivirus感染難轉的細胞株,誘導細胞immortalizing.



















































































Lenti-Myc-T 58A Virus



pLenti-MycT58A



Recombinant Myc T 58A Lentivirus (106cfu/ml).



10ml



G217



Retro-Rev-Tre-Myc-T 58A Virus



pRetro-Rev-Tre-Myc T58A



Recombinant Rev-Tre-MycT 58A Retrovirus (106cfu/ml).



10ml



G224



Lenti-p53 siRNA Virus



iLenti-p53



Recombinant p53 siRNA Lentivirus (106cfu/ml).



10ml



G219



Lenti-Ras-V12 Virus



pLenti-Ras V12



Recombinant Ras V12 Lentivirus (106cfu/ml).



10ml



G221



Lenti-Rb siRNA Virus



iLenti-Rb



Recombinant Rb siRNA Lentivirus (106cfu/ml).



10ml



G223



Lenti-HPV-16 E6/E7 Virus



pLenti-HPV-16 E6/E7



Lentivirus containing HPV-16 E6/E7 gene (106cfu/ml).



10ml



G268



EBV Virus



N/A



Virus containing EBV gene (106cfu/ml).



10ml



G229



Lenti-hTERT Virus



pLenti-hTERT



Lentivirus containing hTERT (106cfu/ml).



10ml



G200



Lenti-hTERT Antisense Virus



pLenti-hTERT Antisense



Lentivirus containing antisense hTERT (106cfu/ml).



10ml



G201



Lenti-III-EFIα-hTERT-YFP Virus



pLenti-EF1α-hTERT-YFP



Lentivirus containing hTERT and YFP reporter (106cfu/ml).



10ml



G276



Adeno-hTERT Virus



pAdeno-hTERT



Adenovirus containing hTERT (106cfu/ml).



250ul



G205



Adeno-hTERT Antisense Virus



pAdeno-hTERT Antisense



Adenovirus containing antisence hTERT (106cfu/ml).



250ul



G208



Retro-E1-hTERT Virus



pRetro-E1 hTERT



Retrovirus containing hTERT (106cfu/ml).



10ml



G207



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【人類初代CD34+細胞】太鼎生物科技有限公司(02)86609496



 


人類初代CD34+細胞 Human Normal PV Bone Marrow CD34+ Primary Cells


 


































Organism



Homo sapiens



Source Organ



Bone Marrow



Growth Properties



Adherent



Morphology



Polygonal



Propagation



Grow cells in T25 ECM-coated flasks(G299) with the following conditions. The base medium for this cell line is Prigrow X medium available in ABM, Cat. No. TM100. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (TM999) to a final concentration of 10% and Penicillin/Streptomycin(G255). Atmosphere: air, 95%; Carbon dioxide (CO2), 5%.



Subculturing



1. Remove and discard culture medium. 2. Add 2.0mL of Trypsin-EDTA solution (TM050) to flask and observe cells under an inverted microscope until cell layer is dispersed. Note: Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. 3. Centrifuge cells at 1500rpm for 3 minutes to pellet. 4. Aspirate out trypsin, leaving pellet undisturbed. 5. Resuspend pellet in fresh culture medium and plate in new culture vessel. 6. Incubate cultures at 37°C . Be sure to use T25 ECM-coated flasks(G299) for growth.



Preservation



1. Freeze Medium: Complete growth medium with 10% DMSO.
2. Storage Temperature: Liquid nitrogen vapour phase.



Notices and Disclaimers



1. ABM products are intended for laboratory research purposes only, unless noted otherwise. They are not intended for use in humans.
2. ABM makes no warranties or representations as to the accuracy of the information on this site. Citations from literature and provided for informational purposes only. ABM does not warrant that such information has been shown to be accurate.



Caution



These Human Normal PV Bone Marrow CD34+ Primary Cells (HNPVBMCD 34C ) are for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information.


 


 



詳細產品資訊,請登錄www.abmgood.com 查詢,或與太鼎生物科技有限公司(02)86609496聯繫。 


更多參考內容:


WWW.BIOPIONEER.COM.TW


 太鼎生物科技有限公司(02)86609496

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進行細胞 immortalization,只須將定量好的SV40 Lentivirus 感染細胞,即可讓細胞immortalization,其不須要任何的抗生素篩選,即可讓primary的細胞成為immortalize細胞株。客戶不須再自己養病毒, 表現病毒及定量病毒力價!!



SV40全名猿猴空泡病毒40Simian vacuolating virus 40)或猿猴病毒40Simian virus 40),是一種多瘤病毒,也是一種DNA病毒,有造成腫瘤發生的潛在能力,不過通常會維持於潛伏感染(latent infection)狀態。這種病毒是在1960年,於用來生產脊髓灰質炎疫苗恆河猴腎臟細胞中發現。



abmgood一系列病毒表現產品. 幫您生產好重組sv40 Lentivirus病毒,且經由QPCR定量Lentiviru的病毒力價( 106 c fu/ml),立即可以感染細胞,即可讓細胞immortalize, 培養液不需添加抗生素, 針對對抗生素敏感的primary cell, 可以cultrure更多代。另有更多相關產品包括hTERT,p53,Myc,Rb,Ras V12, SV40 T antigen等都是做好的lentivirus病毒,可以直接進行細胞感染。



 


產品貨號 Abmgood #G258                太鼎生物科技有限公司代理02-06689496



 








































Product



Vector Map


(Click to View)



Description



Quantity



Cat. #



Adeno-SV40 Adenovirus



pAdeno-SV40



Adenovirus expressing SV40 large and small T antigens (106cfu/ml).



250ul



G210



Retro-SV40 Retrovirus



pRetro-E2-SV40



Retrovirus expressing SV40 large and small T antigens (106cfu/ml).



10ml



G212



Lenti-SV40 Lentivirus



pLenti-SV40



Lentivirus expressing SV40 whole gene (106cfu/ml).



10ml



G203



Lenti-SV40T Lentivirus



pLenti-SV40-T



Lentivirus expressing SV40 large T antigen (106cfu/ml).



10ml



G256



Lenti-SV40 T+t Lentivirus



pLenti-SV40-Tt



Lentivirus expressing SV40 large and small T antigens (106cfu/ml).



10ml



G258



 


 


 


 



業務專員 許 虹宜 0920312382



 


詳細產品資訊,請登錄www.abmgood.com 查詢,或與太鼎生物科技有限公司(02)86609496聯繫。 


WWW.BIOPIONEER.COM.TW


太鼎生物科技有限公司(02)86609496


 


 

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初代細胞 (primary cells) 較一般細胞株 (cell line) 取得不易,且具有繼代培養代數有限和培養基費用較高等…問題,因此Abmgood針對一系列初代細胞、幹細胞等,直接銷售初代培養細胞,滿足即時細胞影像攝影、細胞遷移、傷口癒合、共同培養等…多樣化的實驗設計與需求


 


Products


·       Primary Adipose Cells


·       Primary Airway Cells


·       Primary Blood Cells


·       Primary Blood Vessel Cells


·       Primary Bone & Cartilage Cells


·       Primary Bone Marrow Cells


·       Primary Brain Cells


·       Primary Breast Cells


·       Primary Colon Cells


·       Primary Eye Cells


·       Primary Hair Follicle Cells


·       Primary Heart Cells


·       Primary Liver Cells


·       Primary Mesentery Tissue Cells


·       Primary Oral Cells


·       Primary Pancreas Cells


·       Primary Pharynx Cells


·       Primary Placenta Cells


·       Primary Renal System Cells


·       Primary Reproductive System Cells


·       Primary Skeletal Muscle Cells


·       Primary Skin Cells


·       Primary Umbilical Cord Cells


詳細產品資訊,請登錄www.ABMGOOD.com 查詢,或與太鼎生物科技有限公司(02)86609496聯繫。 


更多參考內容:


WWW.BIOPIONEER.COM.TW


 太鼎生物科技有限公司(02)86609496

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