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目前分類:【Lentivirus慢病毒表現、純化】 (32)

瀏覽方式: 標題列表 簡短摘要

 

TBX18GFP Adenovirus(人類)  

品牌abmgood/貨號#375888A/ 包裝250ul (病毒力價1x106pfu/mL)各力價濃縮加購如下附件

TBX18-GFP Adenovirus (Human)

Service

Description

Cat. No.

Adenovirus Purification

Amplify and purify (2-3 ml of 109 pfu/ml)

C070

Amplify and purify (2-3 ml of 1010 pfu/ml)

C071

Amplify and purify (5 x 200 μl of 1011 pfu/ml)

C072

Amplify and purify (5 x 200 μl of 1012 pfu/ml)

C005

 

TBX18GFP Adenovirus產品應用

This adenovirus is part of abm’s Adenoviral Expression System and can be used directly to transiently over-express your gene of interest in a wide range of host cells. This adenovirus can be used to amplify more adenovirus by transducing HEK293 cells. For enhanced transduction efficiency, the use of ViralPlus (G698) at 1:100 is recommended at the time of transduction.

 

Vector

pAdenoG-IRES-GFP

Vector Size

37302bp

Promoter

CMV

Insert

TBX18

Insert Size

1824 bp

Organism

Human

Accession #

BC132715

Chromosome

6Q14.1-Q15

Tags

GFP reporter (IRES)

Gene Type

Wild Type. Mutants can be constructed via custom services. Please refer to our custom service page or contact our technical support for more info.

Titer

1x106pfu/mL

Storage Buffer

DMEM with 2.5% glycerol

Shipping & Storage

Adenoviruses are stable at room temperature for short term storage and are shipped with gel ice packs. Do not store at -20°C or 4°C for long term. For long term storage, it is recommended to store the viruses at -80°C and avoid repeated freeze-thaw cycles. Before storing at -80°C, it is advisable to make small aliquots to avoid repeated freeze-thaw cycles. Screw cap vials are recommended to decrease chances of the storage buffer desiccating which leads to decrease in infectivity. Diluting the virus is not recommended.

Disclaimer

1. Disclaimer for transcript variants: The provided accession number refers to the transcript (mRNA) sequence for this product. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. All sales are final.
2. The provided accession number refers to the transcript (mRNA) sequence for this product. Please verify that this is the desired transcript sequence by cross-referencing. This is important because a single gene can have multiple different transcripts owing to naturally occurring variations. All sales are final.
3. Disclaimer for intended use: All of abm's vectors and viral particles are for research use ONLY and NOT for therapeutic/diagnostic applications. abm is not liable for any repercussions arising from the use of its vector(s) in therapeutic/diagnostic application(s).
4. Disclaimer for extra nucleotides: Cloning may lead to the insertion of extra nucleotides at the 5' or 3' end of the gene of interest which, in most cases, is innocuous to the stability/functionality and the expression of that gene.

Guarantee

abm guarantees that the correct ORF construct is provided and the mRNA expression is displayed upon successful transduction. If this is not the case, we will provide a one-time replacement. Customers must provide adequate data to show >80% transfection efficiency with a positive control, plus additional qPCR data or a western blot to evaluate the level of gene expression. The replacement will not be covered by the same guarantee.

Please note that due to the large number of variables applicable, any further expression analysis (e.g. protein expression) is not covered by the guarantee, as such analysis is dependent on the end user's experimental conditions.

 

 

引用文獻

Das, A et al. "Engulfment of apoptotic cells by macrophages: a role of microRNA-21 in the resolution of wound inflammation.." J Immunol 192(3):1120-9 (2014). DOI: 10.4049/jimmunol.1300613. PubMed: 24391209 . Application: Transduction.

 

Hai, B et al. "Transient activation of hedgehog pathway rescued irradiation-induced hyposalivation by preserving salivary stem/progenitor cells and parasympathetic innervation.." Clin Cancer Res 20(1):140-50 (2014). DOI: 10.1158/1078-0432.CCR-13-1434. PubMed: 24150232 . Application: Transduction.

 

Reineke, EL et al. "Steroid Receptor Coactivator-2 is a Dual Regulator of Cardiac Transcription Factor Function." J. Biol. Chem 289 (25):17721-17731 (2014). DOI: 10.1074/jbc.M113.539908. PubMed: 24811170. Application: Adenovirus Expression.

 

Gomes, AP et al. "Declining NAD(+) induces a pseudohypoxic state disrupting nuclear-mitochondrial communication during aging." Cell 155(7):1624-38 (2013). DOI: 10.1016/j.cell.2013.11.037. PubMed: 24360282 . Application: Transduction.

 

Hoffman, BE et al. "Development and characterization of a human articular cartilage-derived chondrocyte cell line that retains chondrocyte phenotype.." J Cell Physiol. 222(3):695-702 (2010). DOI: 10.1002/jcp.21994. PubMed: 20020445 . Application: Adenovirus Transduction Protocol; Transduction.

 

更多相關諮詢請洽

台灣代理廠商: 太鼎生物科技有限公司

公司電話: 02-86609496 /0920312382
公司傳真: 02-86609342
公司網站: www.biopioneer.com.tw
連絡業務: 許虹宜 0920312382
連絡信箱: taiding.biotech@msa.hinet.net

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microRNA(miRNA)是一類有大約22個核苷酸組成的非編碼小分子RNA,其廣泛存在於真核生物中。miRNA在個體發育的不同時期及不同組織中有不同的表達模式,在發育和分化中具有重大的調控作用。miRNA的檢測方法主要有Northern Blot 等基於分子雜交的方法,這些方法敏感度低、耗時長、RNA的用量較大。應用Real-Time PCR技術來對miRNA進行檢測,具有快速、特異性強、靈敏度高等優點。Abmgood提供miRNA QPCR偵測套組,一系列產品包括有miRNA primeruniversal primerEvagreen Mastermix等。各種microRNA表現與偵測的產品;其種類包括有miRNA表現載體、miRNA分離與偵測套組,另提供更多研究miRNA的資料來源。  

 

ABM miRNA表現載體

  • GFP miRNA Lentivirus/Vector
  • miRNA Lentivirus/Vector
  • iCumate miRNA Lentivectors right_circular_10px
  • miRNA(microRNA) Adenovirus
  • miRNA Inhibitor Lentivirus/Vector
  • miRNA Inhibitor Adenovirus
  • 3'UTR miRNA Reporter & Cell Line

miRNA萃取與偵測

G269/G270 miRNA cDNA Synthesis Kit Workflow
G269/G270 miRNA cDNA Synthesis Kit Work Flow-ABMgood

產品型號

產品名稱

包裝

G269
G270

miRNA cDNA Synthesis Kit

25 Reactions
100 Reactions

Human miRNA internal control primer

MPH00001

U6-2 Primers

150ul/10uM

MPH00003

SNORD44 Primers

150ul/10uM

MPH00004

SNORD47 Primers

150ul/10uM

MPH00005

SNORD48 Primers

150ul/10uL

 

 

<更多 相關諮詢請洽>

 

代理廠商】: 太鼎生物科技有限公司

【公司電話】: 02-86609496 傳真 : 02-86609342
【公司網站】: www.biopioneer.com.tw
【連絡業務】: 許虹宜 0920312382
【連絡信箱】: taiding.biotech@msa.hinet.net

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品牌abmgood 貨號LV999 規格100ML

Speedy Lentivirus Purification

 

  Recombinant lentiviral vectors have been shown to be a powerful
tool for stable gene transfer to both dividing and non-dividing cells in vitro
and in vivo.Through years of experience with lentiviral vectors, ABM has
developed its own proprietary pLenti-combo packaging mix and an efficient
protocol for rapid production of recombinant lentiviral vectors with titers up
to 107IU/ml. The quickest and easiest way to purify and concentrate
lentiviral particles up to 100x. The concentrated viral preparations can be
used for in vivo injection or like applications that require high titer of
viral particles.

 

 

 


 

 

 

 

 

 

-----------------------------------------------

 

Advantages of ABM's Lentiviral Expression
Vectors

 

1. stable plasmid; rare plasmid DNA re-arrangement

2. very high yielding plasmid

3. no special competent cells required

4. His and HA tag compatible

5. ABM is THE source for your lentiviral expression needs!

 

Lentivirus Purification Protocol Cat. No.: LV999

1. Harvest viral supernatant, spin at 2500g for 10 minutes and filter through a
0.45 um syringe filter.

2. Add 5ml of Lenti-binding solution to 45
ml of the viral supernatant and mix thoroughly by inversion.

3. Centrifuge at >= 5000g at 4C for 10 minutes.

4. Decant the supernatant. Be careful not
to disturbe the pellet.

5. Resuspend the viral pellet in 0.45ml ~
4.5ml of serum-containing medium, depending on the concentration of virus
needed.


 

<更多 相關諮詢請洽>

 

代理廠商】: 太鼎生物科技有限公司

【公司電話】: 02-86609496 傳真 : 02-86609342
【公司網站】: www.biopioneer.com.tw
【連絡業務】: 許虹宜 0920312382
【連絡信箱】: taiding.biotech@msa.hinet.net

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ABM Speedy Lentivirus Purification kit 病毒快速純化套組

 

傳統的病毒純化方法,大多利用超高速離心(ultracentrifuge),搭配濃度梯度(CsCl)等方法進行病毒分離,需要純熟技術且過程費時。病毒純化套組可免除使用超高速離心,更簡單、快速取得高濃度病毒液。

  1. 30分鐘內快速純化病毒。
  2. 可達濃縮效果約100x。
ABM_Speedy
293 cells 感染純化前(A)與純化後(B)的lentivirus 48hr後的結果

----------------------------------------------

ABM qPCR Lentivirus Titration(Titer) Kit

  1. One-Step qPCR,2小時內可完成定量。
  2. Primer經特殊設計,解決primer dimer干擾。
  3. 附有病毒濃度標準液兩組,準確定量病毒濃度。
  4. 搭配ABM qPCR Mastermix,效果更佳。

ABM_lentiviral_titer_workflow

--------------------------------------------

產品編號 產品名稱 包裝
LV999 Speedy Lentivirus Purification 100ml
LV900 qPCR Lentivirus Titration(Titer) Kit 100 Reactions
其他病毒純化相關產品
LV998 Ultra-Pure Lentivirus Purification Kit-可純化病毒濃度達1011病毒顆粒 1 Kit
G171
G172
G173
PuRetro™ Lentivirus Purification Kit 2 Standard Units
5 Standard Units
2 Giga Units

---------------------------------------------------------------

 

<原廠資料連結>

 

<更多 相關諮詢請洽>

 

代理廠商】: 太鼎生物科技有限公司

【公司電話】: 02-86609496 傳真 : 02-86609342
【公司網站】: www.biopioneer.com.tw
【連絡業務】: 許虹宜 0920312382
【連絡信箱】: taiding.biotech@msa.hinet.net

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Lentivirus病毒定量-快篩片

Instant Lentivirus Titration Card慢病毒定量卡使用橫向流方法(lateral flow immunoassay)偵測lentiviral capsule protein進行定量。比起傳統infection based assays需3-4天,Instant Lentivirus Titration Card 只要10分鐘即可得到定量結果。

  • 簡單操作,10分鐘獲得結果-不需額外萃取步驟
  • 目測觀察結果顏色變化
  • 用途:評估培養液中適當的病毒濃度
  • 靈敏度可達1×105 IU/mL病毒濃度(或7.7 pg/ml的p24 viral capsid protein)

ABM_Lenti-Card-figure

產品編號 產品名稱 包裝
G614
G615
Instant Lentivirus Titration Card 10 Tests
20 Tests
病毒純化/定量相關產品
LV999 Speedy Lentivirus Purification 100ml
LV900 qPCR Lentivirus Titration(Titer) Kit 100 Reactions
LV998 Ultra-Pure Lentivirus Purification Kit 1 Kit

 

更多相關諮詢請洽

https://www.abmgood.com/Lentivirus-Instant-Titer-Card.html

台灣代理廠商: 太鼎生物科技有限公司

 

公司電話: 02-86609496 公司傳真: 02-86609342


公司網站: www.biopioneer.com.tw


連絡業務: 許虹宜 0920312382


連絡信箱: taiding.biotech@msa.hinet.net

 

 

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rIRS1-HA Adenovirus




胰島素接受體受脢質-1IRS-1 (Insulin
Receptor Substrate 1
)
在”Insulin ReceptorPI 3-kinase之間的訊息傳遞”中扮演關鍵角色。Abmgood提供定量好之IRS1-HA腺病毒液。1x106pfu/mL





IRS1-HA病毒培養液


 

IRS1-HA病毒培養液】貨號#286878Aabmgood提供定量好之IRS1-HA adenovirus(NM_012969) 胰島素接受體受脢質-1病毒培養液,1x106pfu/mL



















































































Cat.No.:




286878A




Quantity:




250ul




Vector




pAdenoG-HA




Vector Size




32908bp




Promoter




CMV




Insert




rIRS1-HA




Insert Size




3708 bp




Organism




Rat




Accession #




NM_012969




Alias




IRS1IRM




Protein Type




insulin receptor substrate 1




Chromosome




9q34




Tags




HA




Gene Type




Wild Type




Titer




1x106pfu/mL




Storage




DMEM with 2.5% glycerol




Shipping




On dry ice.




Caution




This product is for research use only and is not intended for
therapeutic or diagnostic applications. Please contact a technical service
representative for more information.




Notes




The molecular sequence of this clone aligns with the gene accession
number as a point of reference only. However, individual transcript sequences
of the same gene can differ through naturally occurring variations (e.g.
polymorphisms), each with its own valid existence. This clone is
substantially in agreement with the reference, but a complete review of all
prevailing variants is recommended prior to use. All sales are final






 


[供應廠商】太鼎生物科技有限公司


【連絡人】許虹宜 0920-312382 taiding.biotech@msa.hinet.net



【臺北公司】02-86609496



【相關網址】www.biopioneer.com.tw



【原廠網址】www.abmgood.com

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siRNA Apoptosis Vectors載體





siRNA Apoptosis Vectors







BIOVISON提供ApoptosissiRNA
Apoptosis vectors
。包括AIFBcl2
FamilyCaspase FamilycIAPsHSP-90
siRNA
表現載體


Small
interfering RNAs (siRNAs) are short, double-stranded RNA molecules that can
target and degradate specific complementary mRNAs. The target gene-specific
degradation is an effective means of gene suppression. BioVision's GeneBlocker






















 pGB siRNA expression vectors are designed to
provide efficient suppression of a target gene in cultured mammalian cells and
in vivo
. The pGB vector has been optimized for suppressing expression
of target genes using the human U6 promotor (a RNA polymerase III promotor)
which generates large amounts of siRNA in mammalian cells. The pGB vector
provides neomycin resistance marker for the selection of stable cell lines,
permitting long term suppression of the target gene. BioVision offers siRNA
vectors targeting to important Apoptosis genes. The negative control siRNA
vector and pGB cloning vector for cloning in your own insert are also
available.





AIF









































Catalog #




Product Name+




Size




9511-20




pGB AIF
siRNA Vector Mix




20 µg




9511-60




pGB AIF
siRNA Vector Mix




60 µg






Bcl-2 Family Members





 




















































Catalog #




Product Name+




Size




9512-20




pGB BAD
siRNA Vector Mix




20 µg




9512-60




pGB BAD
siRNA Vector Mix




60 µg




9513-20




pGB BAX
siRNA Vector Mix




20 µg




9513-60




pGB BAX
siRNA Vector Mix




60 µg




9514-20




pGB
Bcl-2 siRNA Vector Mix




20 µg




9514-60




pGB
Bcl-2 siRNA Vector Mix




60 µg




9515-20




pGB BID
siRNA Vector Mix




20 µg




9515-60




pGB BID
siRNA Vector Mix




60 µg






 





Caspase Family Members





 





































Catalog #




Product Name+




Size




9501-20




pGB
Caspase-1 siRNA Vector




20 µg




9501-60




pGB
Caspase-1 siRNA Vector




60 µg




9503-20





pGB
Caspase-3 siRNA Vector




20 µg




9508-20




pGB
Caspase-8 siRNA Vector




20 µg




9509-20




pGB
Caspase-9 siRNA Vector




20 µg






 





cIAPs





 
































Catalog #




Product Name+




Size




9516-20




pGB
CIAP-1 siRNA Vector Mix




20 µg




9516-60




pGB
CIAP-1 siRNA Vector Mix




60 µg




9517-20




pGB
CIAP-2 siRNA Vector Mix




20 µg




9517-60




pGB
CIAP-2 siRNA Vector Mix




60 µg






 





HSP-90





 






















Catalog #




Product Name+




Size




9518-20




pGB
Hsp90 siRNA Vector Mix




20 µg




9518-60




pGB
Hsp90 siRNA Vector Mix




60 µg






 





siRNA Cloning Vectors





 

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Ultra-Pure Lentivirus Purification
Kit


lentivirus病毒純化及濃度套組,有效提高病毒純度及力價






 


重組慢病毒lentivirus,廣泛應用於細胞之轉導。轉染後的病毒上清液力價,約106 CFU菌落形成單位(菌落形成單位)/ ml。在高效能病毒力價及體內基因傳遞中,要求較高的病毒純度及力價,且於活體試驗中,避免病毒細胞培養上清液,所造成之污染可能性。因此,病毒上清需要進一步濃縮及純化。





 Abmgood提供一套病毒純化套組。







傳統純化病毒,經由超速離心濃縮。雖然該方法效果很好,但需要特定的的超速離心設備,且技術要求高。此外,以濃縮的病毒上清總體積是有限的超速離心管的大小。報告指出,超速離心過程中,會造成一些不利的物理效應,會影響病毒顆粒的生物活性。






Abmgood發展 Ultra-Pure
Lentivirus Purification Kit
(Cat. No.
LV998).
針對病毒濃縮技術及純度做改良。提供一個快速且有效,純化及濃縮病毒的產品。其設計原理,為利用離子交換的結合和獨特緩衝液,最大限度減少病毒顆粒的損壞,且提高病毒力價。







Ultra-Pure Lentivirus Purification Kit 套組內容:





(Cat. No. LV998)





LentiBind Buffer A
(320ml)





LentiBind Buffer B
(128ml)





Lenti-Elution buffer
(32ml)






LentiBuffer (40ml)







病毒純化步驟





1. On both day 4
and day 5, centrifuge the collected viral super
natant





at 6000g for 10
minutes at 4
°C to
pellet the cell debris. Transfer





supernatant to a new
centrifuge container without disturbing the cell





debris.





2. For every
100ml of viral supernatant to be purified,
add 20ml of





LentiBind Buffer A and
8.0ml of LentiBind Buffer B. Mix well and shake





the mixture on a
rotating/orbital shaker (commonly used in Western





Blot applications) at
200-300rpm at 4
°C
overnight (for day 4 collection).





3. For day 5
viral supernatant collection, allow the binding for 3





hours only and then
combine both collections before proceeding to





step 4.





4. Centrifuge at
7000g at 4
°C for 30 mins. to collect the viral particles.





5. Discard
supernatant completely without disturbing the pellet,





and spin briefly again
if necessary to remove any residual liquid.





6. Add 8.0ml
Lenti-Elution buffer to resuspend the viral pellet com
pletely





with a transfer
pipette and transfer the viral suspension to a 10ml





sterile tube that is
resistant to 10,000g during centrifugation in the following step (Sarstedt Cat
No. 62.515.006). Shake the tubes at 4
°C for 30min to elute viral particles
from LentiBind Buffer A.





7. After
incubation, spin at 10,000rpm for 20 min to separate viral





particles from the
binding matrix.





8. Transfer the
supernatant (viral particles) to a 10ml syringe and





filter preparation
through a 0.45
μM
filter (Millipore, Cat No. SLHV033RB)





to further clarify the
preparation.





9. Perform buffer
exchange for the viral preparation to get rid of





the high salt
concentrations carried over from LentiBind Buffer B using





a 100kDa cut-off
protein concentrating column (Sartorius stedim biotech,





Cat No. VS2042).





10. Spin the
column at 3000rpm at 20
°C until the viral supernatant





volume is close to
1-2ml. To speed up the process, use 1ml transfer pipette to re-suspend the
viral preparation every 30 minutes.





11. Add 10ml
LentiBuffer and spin the column again at 3000rpm at





20°C to exchange for final
buffer until the final volume is 200
μl-1.0ml





depending on viral
titre desired. To speed up the process, use 1ml





transfer pipette to
re-suspend the supernatant every 30 minutes.





12. Transfer the
final viral preparation from the buffer exchange col
umn





to a 1-3ml syringe and
sterilize it through a 0.45
μM filter. Make sure





to pre-wet the filter
with 100
μl of
LentiBuffer to prevent virus loss caused





by residual liquid
left in the filter.





13. Final
lentivirus preparations can either be used immediately for





cell transduction or
aliquoted into smaller volumes (10-20
μl) for longterm





storage at -75°C.





 





【供應廠商】太鼎生物科技有限公司





【連絡人】許虹宜 0920-312382 taiding.biotech@msa.hinet.net





【臺北公司】02-86609496 【台中公司】0935-229803





【相關網址】
www.biopioneer.com.tw





【原廠網址】www.abmgood.com





 


 


 

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qPCR Lentivirus Titration(Titer) Kit





慢病毒qPCR病毒力價----定量套組















Cat.No.:




LV900




Quantity:




100 Reactions



 


qPCR病毒力價定量---產品介紹





Abmgood提供快速且方便之Lentivirus病毒力價定量套組。其結合一個步驟快速RNA萃取及QPCR技術 (SYBR system),2個小時完成定量。套組內附有reagent mix, primerprimerHIV based vetors為設計的偵測方式。快速完成病毒力價定量分析。不會有NTC量化問題,完全排除NTC訊號。精確定量Lentivirus titer之方法。





The Lentivirus Titration Kit provides a
fast and simple method for titrating lentivirus. This kit employs a quick RNA extraction
step and determines viral RNA using qRT-PCR. The whole assay could be completed
in only 2 hours. The titer primers supplied in this kit are designed for all
HIV-1 based vectors detection. With the help of the on-line titer calculator,
titration of any lentiviral preparation could be easier than ever before.





qPCR病毒力價定量





優點介紹





Advantages of ABM's Lentiviral qPCR Titer Kit





1.    no RNA purification -- saves time and
eliminates inaccuracy





2.    ready-to-use reagent mix -- reduces
variability





3.    qRT-PCR in one step -- more sensitive
and accurate than other methods





4.    no NTC amplification -- our titration
kit is the only one on the market that completely eliminates NTC signal due to
our unique primer design. Similar kits from other companies all give NTC
amplification, which compromise accuracy for viral titration







產品內容























1.       Virus Lysis Buffer



800μl



2.   Reagent-mix (RT reagent & Primer)



1.0ml



3.       STD1 (lentivirus standard 1)



300μl



4.       STD2 (lentivirus standard 2)



300μl





標準品STDlentivirus病毒。


Standard
1
濃度為 5x 10^7 IU/ml. Standard 2 濃度為6.25x10^6
IU/ml.
兩者standards 相差 2^3, 相當於Ct 3次方。







相關商品




LentiVirus病毒濃縮套組





Ultra-Pure Lentivirus Purification Kit





lentivirus病毒純化及濃度套組,有效提高病毒純度及力價












 


重組慢病毒lentivirus,廣泛應用於細胞之轉導。轉染後的病毒上清液力價,約106 CFU菌落形成單位(菌落形成單位)/ ml。在高效能病毒力價及體內基因傳遞中,要求較高的病毒純度及力價,且於活體試驗中,避免病毒細胞培養上清液,所造成之污染可能性。因此,病毒上清需要進一步濃縮及純化。


 


Abmgood提供一套病毒純化套組。




傳統純化病毒,經由超速離心濃縮。雖然該方法效果很好,但需要特定的的超速離心設備,且技術要求高。此外,以濃縮的病毒上清總體積是有限的超速離心管的大小。報告指出,超速離心過程中,會造成一些不利的物理效應,會影響病毒顆粒的生物活性。






Abmgood發展 Ultra-Pure Lentivirus Purification Kit (Cat. No. LV998).針對病毒濃縮技術及純度做改良。提供一個快速且有效,純化及濃縮病毒的產品。其設計原理,為利用離子交換的結合和獨特緩衝液,最大限度減少病毒顆粒的損壞,且提高病毒力價。




Ultra-Pure Lentivirus
Purification Kit


套組內容





(Cat. No. LV998)





• LentiBind Buffer A (320ml)


• LentiBind Buffer B (128ml)


• Lenti-Elution buffer (32ml)


•LentiBuffer (40ml)


更多資料如下連結:





http://tw.myblog.yahoo.com/taiding2000/article?mid=680&next=629&l=f&fid=12


 




【供應廠商】太鼎生物科技有限公司




【連絡人】許虹宜 0920-312382  taiding.biotech@msa.hinet.net




【臺北公司】02-86609496 【台中公司】0935-229803




【相關網址】 www.biopioneer.com.tw




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3'UTR miRNA Reporter (luciferase/GFP二種系統)


另提供建立好之miRNA Reporter細胞株


 



 




One
of the most reliable, quantitative assays for the suppression of target genes
by a specific miRNA is the utilization of a reporter gene like luciferase or
GFP. Target site for a specific miRNA is subcloned downstream of the reporter
gene and co-transfected along with a miRNA expressing vector into cells.
Inhibition of reporter genes by the expressed miRNA as compared with valid
controls proves the regulation of genes with target site at 3'-UTR by the
specific miRNA. To facilitate your research on genes of your interests, we have
developed 3'-UTR library of lentiviral vectors for all human genes down-stream
a reporter gene of either luciferase or GFP. The advantage of lentiviral vector
is its flexibility for either transfection or viral vector infection


 


 

GFP
 
 


 





3'UTR Vectors


































Gene Name



Acce. No.



Vector



Size



Cat.No.



 



 



Blank
(Control) Vector



N/A



pLenti-UTR-Luc-Blank



1ug



m012



 



 



A1BG 3'UTR Lenti-reporter-Luc Vector



NM_130786



pLenti-UTR-Luc



1ug



MT-h00001



 



 





3'UTR Virus





































Gene Name



Acce. No.



Vector



Viral Titer



Size



Cat.No.



 



 



Blank
(Control) Virus



N/A



pLenti-UTR-Luc-Blank



106inf/ml



3.0ml



m013



 



 



A1BG 3'UTR Lenti-reporter-Luc Virus



NM_130786



pLenti-UTR-Luc



106inf/ml



3.0ml



MV-h00001



 



 





3'UTR Vectors


































Gene Name



Acce. No.



Vector



Size



Cat.No.



 



 



Blank
(Control) Vector



N/A



pLenti-UTR-GFP-Blank



1ug



m014



 



 



A1BG 3'UTR Lenti-reporter-GFP Vector



NM_130786



pLenti-UTR-GFP



1ug



MT-h50001



 



 





3'UTR Virus





































Gene Name



Acce. No.



Vector



Viral Titer



Size



Cat.No.



 



 



Blank
(Control) Virus



N/A



pLenti-UTR-GFP-Blank



106inf/ml



3.0ml



m015



 



 



A1BG 3'UTR Lenti-reporter-GFP Virus



NM_130786



pLenti-UTR-GFP



106ifu/ml



3.0ml



MV-h50001



 



 





3'UTR
Luciferase Stable Cell Lines





































Product Name



Acc. No.



Cell Line



Concentration



Size



Cat.No.



 



 



A1BG 3'UTR Luciferase Stable Cell Line



NM_130786



293 Cells



10^6 cells/ml



1.0ml



TU000001



 



 



Blank (Control) 3'UTR Luciferase Stable Cell Line



N/A



293 Cells



106 cells/ml



1.0ml



m018



 



 





3'UTR GFP
Stable Cell Lines





































Product Name



Acc. No.



Cell Line



Concentration



Size



Cat.No.



 



 



A1BG 3'UTR GFP Stable Cell Line



NM_130786



293 Cells



10^6 cells/ml



1.0ml



TU050001



 



 



Blank (Control) 3'UTR GFP Stable Cell Line



N/A



293 Cells



106 cells/ml



1.0ml



m019



 



 





【供應廠商】太鼎生物科技有限公司


【連絡人】許虹宜 0920-312382


【臺北公司】02-86609496 太鼎生物科技 發表在 痞客邦 留言(0) 人氣()

 



2nd Generation
Packaging System



































Product




Vector Map



(Click to View)




Select.
Marker




Quantity




Cat.
#




2nd
Generation Packaging System Mix




pLenti-P2A

pLenti-P2B




N/A




200ul




LV003




GFP
Control Lentivirus




pLenti-GFP




Neomycin




10ml




LV006




β-Gal
Control Lentivirus




Map Unavailable




Neomycin




10ml




 


LV007





pLenti-P 2A







pLenti-P2B



Description




For the production of lentiviral particles, three components are
generally required: 1) a lentiviral vector containing your inserts of interests
(cDNA, shRNA, or miRNA), 2) one or two packaging vectors which contain all
necessary viral structure proteins, 3) an envelope vector expressing Vesicular
Stomatitis Virus (VSV) glycoprotein (G). In general, lentiviral vectors with a
wildtype 5' LTR need the 2
nd(second) generation packaging system because these vectors require TAT
for activation. However, the 2
nd(second) generation packaging mix will also support the production of 3rd(third) lentivral expression vector with a
chimeric 5' LTR. All the lentiviral expression vectors market by ABM are 3
rd(third) generation with a chmeric of RSV
promoter upstream of 5'-LTR.


 


【供應廠商】太鼎生物科技有限公司



【連絡人】許虹宜0920-312382



【台北公司】02-86609496



【相關網址】www.biopioneer.com.tw



【原廠網址】http://www.abmgood.com


 

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Abmgood提供miRNA
GFP
表現載體、miRNA 3’ UTR
reporter-luciferase vector
miRNA inhibitor等產品。




GFP hsa-miR-21 miRNA/microRNA Lentivector












Cat.No.:




mh10276




Quantity:




500ng












































miRNA ID




MI0000077




miRNA
Name




hsa-mir-21




Accession
No.




MI0000077




Genome
Location




Chromosome 17:55273409:55273480 (+ Strand )




Species




Human




Insert
Type




Pre-miRNA




Reporter




GFP




Vector
Name




pLenti-III-mir-GFP




miRNA ID




MI0000077



 


pLenti-III-mir-GFP-Blank


























Cat.No.:




m001




Quantity:




500ng




miRNA Name




Blank




Reporter




GFP




Vector Name




pLenti-III-mir-GFP-Blank



 





miRNA Lentivirus
相關產品


































Kit Name




Size




Cat.No.




Complete Kit for 2nd Generation miRNA




1 Kit




MK001




Complete Kit for 3rd Generation miRNA




1 Kit




MK002




Partial Kit for 2nd Generation miRNA




1 Kit




MK003




Partial Kit for 3rd Generation miRNA




1 Kit




MK004




Ultra-Pure Lentivirus Purification Kit




1 Kit




LV998



 


更多產品問與題, 整理資料如下:


http://tw.myblog.yahoo.com/taiding2000/article?mid=535&next=534&l=f&fid=12







【供應廠商】太鼎生物科技有限公司





【連絡人】許虹宜
0920-312382





【台北公司】02-86609496





【相關網址】
www.biopioneer.com.tw





【原廠網址】http://www.abmgood.com





 


 

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Abmgood提供miRNA
GFP
表現載體、miRNA 3’ UTR
reporter-luciferase vector
miRNA inhibitor等產品。






GFP hsa-miR-16 miRNA/microRNA Lentivector















Cat.No.:




mh11053




Quantity:




500ng











































miRNA ID




MIMAT0000069




miRNA
Name




hsa-miR-16




Accession
No.




MIMAT0000069




Species




Human




Insert
Type




Mature miRNA




Reporter




GFP




Vector
Name




pLenti-III-mico-GFP




miRNA ID




MIMAT0000069




miRNA
Name




hsa-miR-16






pLenti-III-mir-GFP-Blank















 
























Cat.No.:



m001



Quantity:



500ng



miRNA Name



Blank



Reporter



GFP



Vector Name



pLenti-III-mir-GFP-Blank






 


 


 




miRNA Lentivirus
相關產品





































Kit Name



Size



Cat.No.



Complete Kit for 2nd Generation miRNA



1 Kit



MK001



Complete Kit for 3rd Generation miRNA



1 Kit



MK002



Partial Kit for 2nd Generation miRNA



1 Kit



MK003



Partial Kit for 3rd Generation miRNA



1 Kit



MK004



Ultra-Pure Lentivirus Purification Kit



1 Kit



LV998


 

更多產品問與題, 整理資料如下:





http://tw.myblog.yahoo.com/taiding2000/article?mid=535&next=534&l=f&fid=12





【供應廠商】太鼎生物科技有限公司





【連絡人】許虹宜
0920-312382





【台北公司】02-86609496





【相關網址】
www.biopioneer.com.tw





【原廠網址】http://www.abmgood.com





 

太鼎生物科技 發表在 痞客邦 留言(0) 人氣()

Abmgood提供miRNA
3’ UTR reporter-luciferase vector
miRNA inhibitormiRNA表現載體等產品。





PTEN 3'UTR
Lenti-reporter-Luciferase Vector












Cat.No.:




MT-h19140




Quantity:




1ug











































 


Full Name




phosphotriesterase related




Accession
No.




NM_030664




Organism




Human




Chromosome
Loc.




10p12




Vector




pLenti-UTR-Luc




Titer




N/A




Storage




-20°C for up to a year.




User
Manual



























Background
Reading











HMGA1 3'UTR
Lenti-reporter-Luciferase Vector
-Blank












Cat.No.:




m012




Quantity:




1ug





更多產品問與題, 整理資料如下:





http://tw.myblog.yahoo.com/taiding2000/article?mid=535&next=534&l=f&fid=12





【供應廠商】太鼎生物科技有限公司





【連絡人】許虹宜
0920-312382





【台北公司】02-86609496





【相關網址】
www.biopioneer.com.tw





【原廠網址】http://www.abmgood.com





 


 

太鼎生物科技 發表在 痞客邦 留言(0) 人氣()

Abmgood提供miRNA
3’ UTR reporter-luciferase vector
miRNA inhibitormiRNA表現載體等產品。



HMGA1 3'UTR
Lenti-reporter-Luciferase Vector











Cat.No.:

MT-h09952




Quantity:




1ug











































Full Name




high
mobility group AT-hook 1




Accession No.




NM_145899




Organism




Human




Chromosome Loc.




6p21




Vector




pLenti-UTR-Luc




Titer




N/A




Storage




-20°C for up
to a year.




User Manual



























Background Reading











HMGA1 3'UTR
Lenti-reporter-Luciferase Vector
-Blank












Cat.No.:




m012




Quantity:




1ug






 


更多產品問與題, 整理資料如下:





http://tw.myblog.yahoo.com/taiding2000/article?mid=535&next=534&l=f&fid=12





 


【供應廠商】太鼎生物科技有限公司





【連絡人】許虹宜
0920-312382





【台北公司】02-86609496





【相關網址】
www.biopioneer.com.tw





【原廠網址】http://www.abmgood.com





 


 

太鼎生物科技 發表在 痞客邦 留言(0) 人氣()

Abmgood提供miRNA
QPCR
偵測套組,一系列產品包括有miRNA primeruniversal
primer
Evagreen Mastermix等產品。





miRNA Primer Results for: hsa-let -7g
primer

























hsa-let-7g Primers




Human




MIMAT0000414




150ul/10uM




MPH01012




 




 




Universal 3'
miRNA Primer




Universal




N/A




150ul/10uM




MPH00000




 




 















































Cat.No.:




MPH01012




Size:




150ul/10uM




Gene Name




hsa-let -7g




miRNA ID




MIMAT0000414




miRNA
Name




hsa-let -7g




Accession
No.




MIMAT0000414




Species




Human




Storage
conditions




-20°C




Background
Reading


























 


U6-2 miRNA Primers對照組】miRNA(microRNA)
primer

















U6-2
Primers




150ul/10uM




MPH00001




U6
snRNA Primers




150ul/10uM




MPM00002





更多產品問與題, 整理資料如下:





http://tw.myblog.yahoo.com/taiding2000/article?mid=535&next=534&l=f&fid=12







【供應廠商】太鼎生物科技有限公司





【連絡人】許虹宜
0920-312382





【台北公司】02-86609496





【相關網址】
www.biopioneer.com.tw


 


【原廠網址】http://www.abmgood.com





 


 

太鼎生物科技 發表在 痞客邦 留言(0) 人氣()

 


Abmgood提供miRNA
QPCR
偵測套組,一系列產品包括有miRNA primeruniversal
primer
Evagreen Mastermix等產品。






miRNA Primer Results for: hsa-let -7a
primer




























Product Name




Species




Access. No.




Size




Cat. No.




hsa-let-7a Primers




Human




MIMAT0000062




150ul/10uM




MPH01006




Universal 3'
miRNA Primer




Universal




N/A




150ul/10uM




MPH00000











































Cat.No.:

MPH01006




Size:




150ul/10uM




Gene Name




hsa-let -7a




miRNA ID




MIMAT0000062




miRNA Name




hsa-let -7a




Accession No.




MIMAT0000062




Species




Human




Storage conditions




-20°C




Background Reading





























【U6-2 miRNA Primers對照組】miRNA(microRNA) primer 貨號#MPH0001。QPCR定量對照組。


  http://tw.myblog.yahoo.com/taiding2000/article?mid=534&prev=535&next=517&l=f&fid=12













U6-2
Primers



150ul/10uM



MPH00001



U6
snRNA Primers



150ul/10uM



MPM00002




更多產品問與題, 整理資料如下:

http://tw.myblog.yahoo.com/taiding2000/article?mid=535&next=534&l=f&fid=12








【供應廠商】太鼎生物科技有限公司





【連絡人】許虹宜
0920-312382





【台北公司】02-86609496





【相關網址】
www.biopioneer.com.tw





【原廠網址】http://www.abmgood.com





 


 

太鼎生物科技 發表在 痞客邦 留言(0) 人氣()






Abmgood提供miRNA QPCR偵測套組,一系列產品包括有miRNA primeruniversal primerEvagreen Mastermix等產品。




miRNA Primers


All our primers have been validated for accuracy,
extensively studied to obtain their optimal qPCR reaction conditions and
optimized to work with our qPCR miRNA Mastermix. Also, they cover every miRNA
characterized in the Sanger 16.0 database release (September 2010).






U6-2 Primers












Cat.No.:




    MPH00001




Size: 




    150ul/10uM



 



























Gene Name 


    U6-2




miRNA ID




    NR_002752.1




miRNA Name




    U6-2




Accession No.




    NR_002752.1




Species




    Human



 


























【供應廠商】太鼎生物科技有限公司




【連絡人】許虹宜 0920-312382




【台北公司】02-86609496




【相關網址】
www.biopioneer.com.tw




【原廠網址】http://www.abmgood.com




更多microRNA問與答資料整理:


http://tw.myblog.yahoo.com/taiding2000/article?mid=535&next=534&l=f&fid=12




 

太鼎生物科技 發表在 痞客邦 留言(0) 人氣()


CD20 (MS4A1)抗體、抗原、siRNA、3'UTR reporter


 


【供應廠商】太鼎生物科技有限公司



【連絡人】許虹宜 0920-312382



 

 


MS 4A 1抗體












CD20
/ MS4A1 (C Terminus)




200ul




100µg/200ul




Y212762





MS 4A 1
siRNA lentivirus










































Product




Accession
Number




Size




Cat.
No.




MS4A1-set




NM_021950




4x500ng




i014282




MS4A1




NM_021950




500ng




i 014282a




MS4A1




NM_021950




500ng




i014282b




MS4A1




NM_021950




500ng




i 014282c




MS4A1




NM_021950




500ng




i014282d





MS 4A 1  lentivirus


















pLenti-CMV-m-Ms4a1




NM_007641.5




1ug




LV031490




pLenti-CMV-rat-Ms4a1




NM_001107578.1




1ug




LV054901





MS 4A 1
Lysate





















 
hMS4A1
Positive Lysate



100ug




PL035497




 
hMS4A1
Positive Lysate with C-Ha Tag



100ug




PL035498




 
rMs4a1
Positive Lysate



100ug




PL138585





MS 4A 1
蛋白質














































 
hMS4A1
Recombinant Protein



5ug




RP070993




 
hMS4A1-C
HA Tag Recombinant Protein



5ug




RP070994




 
hMS4A1-N
His Tag Recombinant Protein



5ug




RP070995




 
hMS4A1-C
His Tag Recombinant Protein



5ug




RP070996




 
rMs4a1
Recombinant Protein



5ug




RP277169




 
rMs4a1-C
HA Tag Recombinant Protein



5ug




RP277170




 
rMs4a1-N
His Tag Recombinant Protein



5ug




RP277171




 
rMs4a1-C
His Tag Recombinant Protein



5ug




RP277172





MS 4A 1
3’ UTR Reporter











































 
MS4A1
3'UTR Lenti-reporter-Luc Vector



Human







1ug




MT-h14733




 
MS4A1
3'UTR Lenti-reporter-Luc Virus



Human







3.0ml




 
MS4A1
3'UTR Lenti-reporter-GFP Virus



Human







3.0ml




MV-h64733





MS 4A 1
protein vector




















pPB-hMS4A1-His




BC002807




1ug




PV070993




pPB-His-hMS4A1




BC002807




1ug




PV070994




pPM-hMS4A1-HA



<font face="&#</body></html>

太鼎生物科技 發表在 痞客邦 留言(0) 人氣()




miRNA(microRNA) Expression &
Detection





miRNA表現與偵測套組-GFP系統






miRNA表現載體letimiRa-GFP


GFP miRNA Lentivirus/Vector (LentimiRa-GFP)




重組慢病毒表達載體(Lentiviral expression vectors); 具有高效率轉譯,及穩定插入基因之特點。因此,lentiviral 表現載體為miRNA delivery之主要工具。的交付使用最廣泛的運載工具。Abmgood提供全系列miRNA慢病毒表達載體(lentimira)。lenrimira具有會功能性之設計,包含CMV啟動子及GFP報導基因,另有定量好的病毒顆粒。每個miRNA Native Pri-miRNA之設計(〜400-500bp的),具有最佳之目標基因抑制效果。

Recombinant lentiviral expression vectors are the most widely used delivery vehicle for miRNA delivery due to their high efficiency transduction and stable integration. As a world leader in both lentiviruses and miRNA, ABM provides the most comprehensive selection of miRNA lentivirus expression vectors (lentimira). Each vector has been tested for functionality and contains a GFP reporter under a constitutive CMV promoter in both plasmid vector and ready-to-use viral particle formats. The design of native pri-miRNA (~400-500bp) for each miRNA offers higher levels of target gene suppression than those based on a single common miRNA backbone as seen with competitors. Please open the following (link or PDF file) for comparison.


Recombinant
lentiviral expression vectors are the most widely used delivery vehicle for
miRNA delivery due to their high efficiency transduction and stable
integration. As a world leader in both lentiviruses and miRNA, ABM provides the
most comprehensive selection of miRNA lentivirus expression vectors
(lentimira). Each vector has been tested for functionality and contains a GFP
reporter under a constitutive CMV promoter in both plasmid vector and
ready-to-use viral particle formats. The design of native pri-miRNA
(~400-500bp) for each miRNA offers higher levels of target gene suppression
than those based on a single common miRNA backbone as seen with competitors.
Please open the following (link or PDF file) for comparison.





 






Human GFP miRNA Lentivirus/Vector
(LentimiRa-GFP) Vector







































miRNA
Name




Vector




Viral
Titer




Size




Cat.No.




Status




Cloning Vector




pLenti-III-mir-GFP-Cloning




N/A




500ug




m016




In Stock




Blank (Control)




pLenti-III-mir-GFP-Blank




N/A




500ng




m001




In Stock




GFP hsa-let-7a-1
Lentivector




pLenti-III-mir-GFP




N/A




500ng




mh10001




In Stock







 


human GFP miRNA定量好的表現病毒


(LentimiRa-GFP) Virus


 


Human GFP miRNA Lentivirus/Vector
(LentimiRa-GFP) Virus































miRNA
Name




Vector




Viral
Titer




Size




Cat.No.




Status




Blank (Control)




pLenti-III-mir-GFP-Blank




107
pfu/ml




2 x
50ul




m002




In Stock




GFP hsa-let-7a-1
Lentivirus




pLenti-III-mir-GFP




107
pfu/ml




2 x
50ul




mh15001




Back Order





LentimiRa-GFP-hsa-let-7a-1 Virus




Cat.No.:mh15001
Quantity:2 x 50ul

 




miRNA IDMI0000060
miRNA Namehsa-let-7a-1
Accession No.MI0000060
Genome LocationChromosome 9:95978060:95978139 (+ Strand)
SpeciesHuman
Insert TypePre-miRNA
ReporterGFP
Vector NamepLenti-III-mir-GFP
Vector Map
Viral Titer107 pfu/ml

 


 


miRNA Lentivirus分析套組



Kits for miRNA Lentivirus Production



































Kit
Name




Size




Cat.No.




Complete Kit for 2nd Generation miRNA




1
Kit




MK001




Complete Kit for 3rd Generation miRNA




1
Kit




MK002




Partial Kit for 2nd Generation miRNA




1
Kit




MK003




Partial Kit for 3rd Generation miRNA




1
Kit




MK004




Ultra-Pure Lentivirus Purification Kit




1
Kit




LV998






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原廠網站: www.abmgood.com


公司網站: www.biopioneer.com.tw


 


 

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