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目前分類:【藥物篩選、癌症行為、抑制劑】 (43)

瀏覽方式: 標題列表 簡短摘要

GTPase分析套組產品介紹 

Innova Biosciences 提供之GTPase分析套組,可探討酵素活性是否扮演transportsignal transduction,protein biosynthesiscell differentiation之角色。應用於GTPase活性分析。提供high-throughput screen 大量GTPase inhibitor。可建立快速藥物篩選系統。內附有GTPase inhibitorGTPase酵素活性分析,採用超高靈敏度Malachite green磷酸根呈色方法,克服傳統呈色方法,低靈敏度限制。測量目標樣本中, GTPases酵素活性

GTPases酵素活性之分析

產品特點

1. 提供high-throughput screen 大量GTPase inhibitor

2. GTPase酵素分析套組,包括stabilizing solution,可避開acid labile substrate所造成的高背景值,其背景值很低(
1mM )

3. 其分析波段為600~660nm,避開一般colored compounds之干擾。

4. Pi-dye複合物極為穩定,其訊號強度可維持數小時之久。

5. Superior呈色系統。採用Malachite Green磷酸根呈色方式,呈色靈敏度比傳統呈色方法高,克服低靈敏度的限制。

6. 適用於大部份的assay buffer

7. 蛋白質濃度高時,不會抑制顏色的形成。

-------------------------------

產品內容物

1 x 10ml of PiColorLock™ Gold* (1 x 25ml)

1 x 0.25ml of Accelerator (1 x 0.5ml)

1 x 5ml of Stabiliser (1 x 10ml)

1 x 1.5ml of 0.1M MgCl2 (2 x 1.5ml)

1 x 5ml of 0.5M Tris pH 7.5 (1 x 10ml)

1 x 5ml of 0.1mM Pi standard (1 x 10ml)

更多參考內容: 原廠連結: http://www.innovabiosciences.com/

 

訂購貨號# 602-0120,196 assays (靈敏度4~40uM)

訂購貨號# 602-0121,480 assays (靈敏度4~40uM)

訂購貨號# 602-0122,480*5 assays (靈敏度4~40uM)

 

GTPase酵素活性分析套組---文獻發表

GTPase酵素活性分析套組

 

The selenocysteine-specific elongation factor
contains a novel and multi-functional domain
- The
Journal of Biological Chemistry (2012)

 

Tubulin nucleotide status controls Sas-4-dependent
pericentriolar material recruitment
- Nature Cell
Biology (2012)

 

The Mitochondrial Phosphatase PGAM5 Functions at
the Convergence Point of Multiple Necrotic Death Pathways

- Cell (2012)

 

Leucyl-tRNA Synthetase Is an Intracellular
Leucine Sensor for the mTORC1-Signaling Pathway
- Cell
(2012)

 

 

GTPase Activity and Neuronal Toxicity of
Parkinson's Disease–Associated LRRK2 Is Regulated by ArfGAP1

- PLoS Genetics (2012)

 

AIP1 Functions as Arf6-GAP to Negatively
Regulate TLR4 Signaling
- JBC (2010)

 

GTPase activity plays a key role in the
pathobiology of LRRK2
- PLoS Genetics (2010)

 

Role of the
G-protein and tyrosine kinase—Rho/ROK pathways in
15-hydroxyeicosatetraenoic acid induced pulmonary vasoconstriction in
hypoxic rats
- J Biochem (2010)

 

AIP1 Functions as Arf6-GAP to Negatively
Regulate TLR4 Signaling
- JBC (2010)

 

Identification and Characterization of a
Leucine-Rich Repeat Kinase 2 (LRRK2) Consensus Phosphorylation Motif

- PLoS One (2010)

 

Speckled-like Pattern in the Germinal Center
(SLIP-GC), a Nuclear GTPase Expressed in Activation-induced
Deaminase-expressing Lymphomas and Germinal Center B Cells

- JBC (2009)

 

High-level production and characterization of a
G-protein coupled receptor signaling complex
-
FEBS Journal (2009)

 

Structure of ERA in
complex with the 3′ end of 16S rRNA: Implications for ribosome biogenesis

-
PNAS (2009)

 

G Protein-Coupled Receptor-Promoted Trafficking
of Gβ1γ2 Leads to AKT Activation at Endosomes via a Mechanism Mediated by
Gβ1γ2-Rab11a Interaction
Molecular Biology of
the Cell (2008)

 

Characterization
of Human GTPBP3, a GTP-Binding Protein Involved in Mitochondrial tRNA
Modification
- Molecular and Cellular Biology
(2008)

 

Comparative real-time PCR and enzyme analysis of
selected gender-associated molecules in
Schistosoma japonicum

- Parasitology (2008)

更多相關諮詢請洽

台灣代理廠商: 太鼎生物科技有限公司

業務代表: 許虹宜 0920312382 email: taiding.biotech@msa.hinet.net

公司電話: 02-86609496傳真: 02-86609342
公司網站: www.biopioneer.com.tw

 

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癌化特性之分析套組-Tube formation kit

trevigen提供 Cultrex® In Vitro Angiogenesis Assay Kit ,可以偵測血管細胞血管形成之inducersinhibitors分析。微孔板上預包埋Trevigen專利的BME基質及其它組分蛋白,具有極低的細胞毒性,經嚴格的無細菌/真菌/支原體等驗證,接近真實體內環境;螢光定量具更高的精準度


 

Cultrex® In Vitro Angiogenesis Assay Tube Formation Kit

Catalog #: 3470-096-K 體外血管新生分析套組

 

癌化過程中具有血管新生之過程,藉以提供癌細胞養份及氧氣血管新生作用是由內皮細胞所引導的過程中細胞會分泌酵素來分解基底膜造成細胞之遊走經由一連串的增生分化後最後形成管狀般的血管構造。trevigen提供 Cultrex® In Vitro Angiogenesis Assay Kit ,可以偵測血管細胞血管形成之inducers及inhibitors分析。

Cultrex® In Vitro Angiogenesis Assay Tube Formation Kit

 

Under permissive
conditions, endothelial cells respond by self organizing into structures that
resemble microvessels or tubes. Trevigen's Cultrex® In Vitro Angiogenesis Assay
Kit allows for the detection of inducers and inhibitors of endothelial cell tube
formation. Cultrex
® Reduced Growth Factor (RGF) Basement Membrane
Extract (BME) is a soluble form of basement membrane purified from
Engelbreth-Holm-Swarm (EHS) tumor, which gels at room temperature to form a
reconstituted protein matrix comprised mainly of laminin, collagen IV,
entactin, and heparin sulfate proteoglycan. Sulforaphane [1-
isothiocyanato-(4R)- methylsulfinyl)-butane], found in broccoli and other
cruciferous vegetables, is a naturally occurring cancer chemopreventive agent,
and is provided as a control for inhibition of in vitro endothelial cell tube
formation on Cultrex
® RGF BME. Calcein AM is provided for rapid and
accurate measurement of cell viability and/or cytotoxicity, and kinetic
analysis of tube formation. Trevigen's Cultrex
® In Vitro
Angiogenesis Assay Kit provides a cost-effective method for investigation of
prospective angiogenesis inhibitors in a 96 well plate format.

 

COMPONENTS:

Basement Memrane Extract (BME) growth (3433-005-01)
factor reduced

Calcein AM  (螢光顯微鏡)

Cell staining solution (倒立顯微鏡/相位差顯微鏡)

Sulphoraphane 10mM

100 µM Sulforaphane working solution

 

REFERENCES:

 

1. Folkman J.
2007. Angiogenesis: an organizing principle for drug discovery? Nat. Rev. Drug
Discov. 6:273-286.

2. Jaffe EA, Nachman RL, Becker CG, Minick CR. 1973. Culture of human
endothelial cells derived from umbilical veins. J. Clin. Invest. 52:2745-2756.

3. Folkman J, Haudenschild C. 1980. Angiogenesis in vitro. Nature 288:551- 556.

4. Kleinman HK, McGarvey ML, Hassell JR, Star VL, Cannon FB, Laurie GW, Martin
GR. 1986. Basement membrane complexes with biological activity. Biochemistry
25:312-318.

5. Lawley TJ, Kubota Y. 1989. Induction to morphologic differentiation of
endothelial cells in culture. J. Invest. Dermatol. 93:59S-61S.

6. Ucuzian AA, Greisler HP. 2007. In vitro models of angiogenesis. World J.
Surg. 31:654-663.

7. Salcedo R, Zhang X, Young HA, Michael N, Wasserman K, Ma W-H, Martins-Green
M, Murphy WJ, Oppenheim JJ. 2003. Angiogenic effects of prostaglandin E2 are
mediated by up-regulation of CXCR4 on human microvascular endothelial cells.
Blood 102:1966-1977.

8. Mochizuki M, Philp D, Hozumi K, Suzuki N, Yamada Y, Kleinman HK, Nomizu M.
2007. Angiogenic activity of syndecan-binding laminin peptide AG73
(RKRLQVQLSIRT). Arch. Biochem. Biophys. 459:249-255.

9. Arnaoutova, I. , J. George, H.K. Kleinman,
and G. Benton. 2009. The endothelial cell tube formation assay on basement
membrane turns 20: state of the science and the art. Angiogensis. 12(3):267-74.

10. Arnaoutova I, and Kleinman HK. 2010. In vitro angiogenesis: endothelial
cell tube formation on gelled basement membrane extract. Nat Protoc.
5(4):628-35.

 

 

 

Kit Components:

 

更多原廠資料連結


http://www.trevigen.com/item/274/Cultrex_In_Vitro_Angiogenesis_Assay_Tube_Formation_Kit/ 

TrevigenCultrex體外血管生成分析試劑盒可進行內皮細胞管形成誘導劑和抑制劑的檢測.不含生長因數(RGF)的BME是從EHS腫瘤中純化出的可溶形式基底膜,其在室溫條件下凝固形成由層連蛋白、型膠原蛋白、Entactin蛋白以及肝素硫酸鹽糖蛋白形成的重構蛋白基質.在花椰菜和其他十字花科蔬菜中發現的萊菔硫烷,是一種天然的癌症化學抑制劑,被用於體外內皮細胞在Cultrex BME上血管形成的抑制劑陰性對照.鈣黃綠素-AM用於快速準確檢測細胞的生存能力和/或細胞毒性,以及血管形成的即時動力學分析.Trevigen Cultrex體外血管生成分析試劑盒以96孔方式為檢測潛在的血管生成抑制劑提供高效的方案.

 

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ROCK抑制劑(A Rho kinase) 體用包裝5mg

 

品牌BIOVISION/貨號#1784-5/ 包裝5MG (純度>98%)

Y-27632ROCK (A Rho kinase)之抑制劑。Y-27632 is a cell-permeable, highly potent and selective inhibitor of Rho-associated, coiled-coil containing protein kinase (ROCK). Y-27632 inhibits both ROCKI (Ki = 220 nM) and ROCKII (Ki = 300 nM) by competing with ATP for binding to the catalytic site. (Davies et al., Ishizaki et al.)

 

Y-27632產品應用

體外試驗或體內試驗, 抑制ROCK酵素活性

 

Y-27632產品提供

Liquid 10mM (5MG) 純度>98%

 

Y-27632產品內容相關

 

內容相關http://www.biovision.com/ezsolution-y-27632-4248.html

Product Name: EZSolution™ Y-27632

Alternate Name/Synonyms: trans-4-[(1R)-1-Aminoethyl]-N-4-pyridinylcyclohexanecar boxamide dihydrochloride

Description: A 10 mM (5 mg in 1550 µl) solution of Y-27632, Dihydrochloride (Cat. No. 1596-5) in deionized water.

Peptide Sequence: N/A

Appearance: Liquid

Formulation: 10 mM (HO)

CAS Number: 146986-50-7

Molecular Formula: C₁₄H₂₁NO.2HCl

Molecular Weight: 320.26

Purity: 98% by HPLC

Solubility:N/A

Storage Temp.: -20°C

Shipping Conditions: gel pack

Handling: Protect from air and light

SMILES: CC(C1CCC(CC1)C(=O)NC2=CC=NC=C2)N.Cl.Cl

InChi: InChI=1S/C14H21N3O.2ClH/c1-10(15)11-2-4-12(5-3-11)14(18)17-13-6-8-16-9-7-13;;/h6-12H,2-5,15H2,1H3,(H,16,17,18);2*1H/t10-,11?,12?;;/m1../s1

InChi Key: IDDDVXIUIXWAGJ-DDSAHXNVSA-N

PubChem CID: 9901617

MDL Number: MFCD03490488

USAGE: For Research Use Only! Not For Use in Humans.

 

其它抑制劑相關如下連結

免疫抑制劑商品>>>>

http://taiding2000.pixnet.net/blog/post/19556158

Bcl-2抑制劑商品>>>>

http://taiding2000.pixnet.net/blog/post/329072982  

 

更多相關諮詢請洽

台灣代理廠商: 太鼎生物科技有限公司

公司電話: 02-86609496 /0920312382
公司傳真: 02-86609342
公司網站: www.biopioneer.com.tw

連絡業務: 許虹宜 0920312382
連絡信箱: taiding.biotech@msa.hinet.net

 

 

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ABT-263, B-細胞淋巴瘤基因Bcl-2抑制劑

Bcl-2, B-細胞淋巴瘤基因, 抑制劑如ABT-265。貨號2467-25

2467.gif

Product Name: ABT-263 An inhibitor of antiapoptotic Bcl-2 proteins

Alternate Name/Synonyms: (R)-4-(4-((4-chloro-4,4-dimethyl-3,4,5,6-tetrahydro-[1,1-biphenyl]-2-yl)methyl)piperazin-1-yl)-N-((4-((4-morpholino-1-(phenylthio)butan-2-yl)amino)-3-((trifluoromethyl)sulfonyl)phenyl)sulfonyl)benzamide; Navitoclax

Description: A potent, small molecule Bcl-2 family protein inhibitor (Ki’s of <1 nmol/L for Bcl-2, Bcl-XL and Bcl-w). Displays anticancer properties. Selectively binds to apoptosis suppressor proteins Bcl-2, Bcl-XL, and Bcl-w and prevents their binding to the apoptotic effectors Bax and Bak proteins, which may trigger apoptosis in tumor cells overexpressing Bcl-2, Bcl-XL, and Bcl-w.

Peptide Sequence: N/A

Appearance: White to off-white solid

Formulation: N/A

CAS Number: 923564-51-6

Molecular Formula: C45H55ClF3N5O6S3

Molecular Weight: 974.61

Purity: ≥98% by HPLC

Solubility:DMSO (> 50 mg/ml)

Storage Temp.: -20 ̊C

Shipping Conditions: gel pack

Handling: Protect from air and light

SMILES: CC1(CCC(=C(C1)CN2CCN(CC2)C3=CC=C(C=C3)C(=O)NS(=O)(=O)C4=CC(=C(C=C4)NC(CCN5CCOCC5)CSC6=CC=CC=C6)S(=O)(=O)C(F)(F)F)C7=CC=C(C=C7)Cl)C

InChi: InChI=1S/C47H55ClF3N5O6S3/c1-46(2)20-18-42(34-8-12-37(48)13-9-34)36(31-46)32-55-22-24-56(25-23-55)39-14-10-35(11-15-39)45(57)53-65(60,61)41-16-17-43(44(30-41)64(58,59)47(49,50)51)52-38(19-21-54-26-28-62-29-27-54)33-63-40-6-4-3-5-7-40/h3-17,30,38,52H,18-29,31-33H2,1-2H3,(H,53,57)/t38-/m1/s1

InChi Key: JLYAXFNOILIKPP-KXQOOQHDSA-N

PubChem CID: 24978538

MDL Number: N/A

USAGE: For Research Use Only! Not For Use in Humans

leftlogo.gif

 

商品如下連結

WWW.BIOVISION.COM

http://www.biovision.com/abt-263-7866.html

 

相關諮詢請洽

台灣代理廠商: 太鼎生物科技有限公司
公司電話: 02-86609496 /0920312382
公司傳真: 02-86609342
公司網站: www.biopioneer.com.tw
連絡業務: 許虹宜 0920312382
連絡信箱: taiding.biotech@msa.hinet.net

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Rapamycin 免疫抑制劑, 5g





Rapamycin





Catalog#: 1568-5  | Size: 5
mg





Rapamycin作用





Rapamycin,雷帕黴素,是新型大環內酯的抗排斥藥物,是目前世界上最新的強效免疫抑制劑,臨床上用於器官移植的抗排斥反應和自身免疫性疾病的治療。可能通過阻斷IL-2啟動的T細胞增殖而選擇性抑制T細胞,用於抗移植排斥反應。20097月的《Nature》雜誌上,文章Rapamycin fed from 20 months of
age extends lifespan in genetically heterogeneous mice
,研究顯示此類藥物能延長酵母菌、線蟲、果蠅等無脊椎動物的壽命。(資料來源http://www.ebiotrade.com/newsf/2010-2/2010223171552934.htm)




 


 


產品資料





Alternate
Name/Synonyms:
23,27-Epoxy-3H-pyrido[2,1-c][1,4]oxaazacyclohentriacontine,
AY 22989, Sirolimus





Appearance: Off-white solid





Formulation: N/A





CAS Number: 53123-88-9





Molecular
Formula:
C₅₁H₇₉NO₁₃





Molecular
Weight:
914.19





Purity: ≥99%





Solubility: DMSO and EtOH





Storage Temp.: -20°C





Shipping
Conditions:
gel
pack





Description: An immunosuppressant.
Selectively blocks signaling that leads to p70 S6 kinase activation (IC50 = 50
pM). Inhibits lymphokine-induced cell proliferation at the G1 phase. Induce apoptosis
in a murine B cell line. Rapamycin has been used in vivo studies by i.p., i.m.,
and i.v. administrations..





Handling: Protect from light and
moisture





 





bIOVISON之相關產品





Apoptosis
Detection Kits & Reagents





 Annexin V Kits & Bulk Reagents





 Caspase Assay Kits & Reagents





 Mitochondrial Apoptosis Kits & Reagents





 Nuclear Apoptosis Kits & Reagents





 Apoptosis Inducers & Inhibitors





 Apoptosis siRNA
Vectors





 





Cell
Fractionation System





 Mitochondria/Cytosol Fractionation Kit





 Nuclear/Cytosol Fractionation Kit





 Membrane Protein Extraction Kit





 Cytosol/Particulate Rapid Separation Kit





 Mammalian Cell Extraction Kit





 FractionPREP
Fractionation System





 





Cell
Proliferation & Senescence





 Quick Cell Proliferation Assay Kit





 Senescence Detection Kit





 High Throughput Apoptosis/Cell Viability Assay Kits





 LDH-Cytotoxicity Assay Kit





 Bioluminescence Cytotoxicity Assay Kit





 Live/Dead Cell
Staining Kit





 





Cell
Damage & Repair





 HDAC Fluorometric & Colorimetric Assays &
Drug Discovery Kits





 HAT Colorimetric Assay Kit & Reagents





 DNA Damage Quantification Kit





 Glutathione &
Nitric Oxide Fluorometric & Colorimetric Assay Kits





 





Signal
Transduction





 cAMP & cGMP Assay Kits





 Akt & JNK Activity Assay Kits





 Beta-Secretase
Activity Assay Kit





 





Adipocyte
& Lipid Transfer





 Recombinant Adiponectin, Survivin, & Leptin





 CETP Activity Assay & Drug Discovery Kits





 PLTP Activity Assay & Drug Discovery Kits





 Cholesterol
Quantification Kit





 





Molecular
Biology & Reporter Assays





 siRNA Vectors





 Cloning Insert Quick Screening Kit





 Mitochondrial & Genomic DNA Isolation Kits





 5 Minutes DNA Ligation Kit





 20 Minutes Gel
Staining/Destaining Kit





 





Growth
Factors and Cytokines (many)





Polyclonal and
Monoclonal Antibodies





 





【連絡人】許虹宜 0920-312382 Hung-Yi Hsu





E-mailtaiding.biotech@msa.hinet.net





【公司電話】02-86609496【公司傳真】02-86609342





【公司網址】www.biopioneer.com.tw





【原廠網址】www.biovision.com





 


 

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DUB相關抑制劑/Spautin-1 貨號#2037-5





Spautin-1

An USP10 and USP13 inhibitor





Product Name: Spautin-1





Alternate Name/Synonyms: 6-Fluoro-N-(4-fluorobenzyl)quinozaline-4-amine





Description: Cell-permeable. A specific and potent
autophagy inhibitor in mammalian cells. It promotes the degradation of Vps34
PI3 kinase complexes by inhibiting two ubiquitin specific peptidases, USP10 and
USP13, that target the Beclin1 subunit of Vps34 complexes. Spautin-1 blocks the
prosurvival autophagy pathway in cancer cells.





Peptide Sequence: N/A





Appearance: White solid





Formulation: N/A





CAS Number: 1262888-28-7





Molecular Formula: C15H11F2
N
3





Molecular Weight: 271.26





Purity: ≥97% by HPLC





Solubility: DMSO (~ 100 mM)





Storage Temp.: -20°C





Shipping Conditions: Gel pack





Handling: Protect from air and light


 







--其它DUB抑制劑--





RP-619  貨號#2302-5





http://tw.myblog.yahoo.com/taiding2000/article?mid=1162&prev=1163&next=1156





LDN-57444 貨號#2016-5





http://tw.myblog.yahoo.com/taiding2000/article?mid=1163&prev=1164&next=1162
 





P22077 貨號#2301-5





http://tw.myblog.yahoo.com/taiding2000/article?mid=1164&prev=1165&next=1163





IU-1 貨號#2301-5





http://tw.myblog.yahoo.com/taiding2000/article?mid=1166&prev=1167&next=1165





WP-1130 貨號#2014-5





http://tw.myblog.yahoo.com/taiding2000/article?mid=1167&prev=1168&next=1166





Spautin-1 貨號#2037-5





http://tw.myblog.yahoo.com/taiding2000/article?mid=1168&prev=-1&next=1167





DUB相關抑制劑





 





RELATED PRODUCTS:





LDN-57444 (Cat. No. 2016-5, 25)





Spautin-1 (Cat. No. 2037-5, 25)





USP14 Inhibitor, IU-1 (Cat. No. 1845-5, 25)





USP7/USP47 Inhibitor P22077 (Cat. No. 2301-5, 25)





WP-1130 (Cat. No. 2014-5, 25)





 


 


【連絡人】許虹宜 0920-312382 Hung-Yi Hsu





E-mailtaiding.biotech@msa.hinet.net





【公司電話】02-86609496【公司傳真】02-86609342





【相關網址】www.biovision.com





【公司網址】www.biopioneer.com.tw





 


 

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DUB相關抑制劑/WP-1130 貨號#2014-5





WP-1130

A selective DUB (deubiquitinases) inhibitor





WP1130 (degrasyn) is a selective deubiquitinase
(DUB)
inhibitor and also inhibits Bcr/Abl with IC50 of 1.8
μM.





Product Name: WP-1130





Alternate Name/Synonyms: (S,E)-3-(6-Bromopyridin-2-yl)-2-cyano-N-(1-phenylbutyl)acrylamide;
Degrasyn





Description: Cell-permeable. WP1130 acts as a
partly selective DUB (deubiquitinases) inhibitor, directly inhibiting DUB
activity of USP9x, USP5, USP14, and UCH37, which are known to regulate survival
protein stability and 26S proteasome function. WP1130-mediated inhibition of
tumor-activated DUBs results in downregulation of antiapoptotic and
upregulation of proapoptotic proteins, such as MCL-1 and p53. Blocks JAK2
signaling via JAK2 ubiquitination.





Peptide Sequence: N/A





Appearance: Pale yellow solid





Formulation: N/A





CAS Number: 856243-80-6





Molecular Formula: C19H18BrN3O





Molecular Weight: 384.27





Purity: ≥98% by TLC





Solubility: DMSO (40 mg/ml) or EtOH (25 mg/ml)





Storage Temp.: -20°C





Shipping Conditions: Gel pack





Handling: Protect from air and light


 







--其它DUB抑制劑--





RP-619  貨號#2302-5





http://tw.myblog.yahoo.com/taiding2000/article?mid=1162&prev=1163&next=1156





LDN-57444 貨號#2016-5





http://tw.myblog.yahoo.com/taiding2000/article?mid=1163&prev=1164&next=1162
 





P22077 貨號#2301-5





http://tw.myblog.yahoo.com/taiding2000/article?mid=1164&prev=1165&next=1163





IU-1 貨號#2301-5





http://tw.myblog.yahoo.com/taiding2000/article?mid=1166&prev=1167&next=1165





WP-1130 貨號#2014-5





http://tw.myblog.yahoo.com/taiding2000/article?mid=1167&prev=1168&next=1166





Spautin-1 貨號#2037-5





http://tw.myblog.yahoo.com/taiding2000/article?mid=1168&prev=-1&next=1167





DUB相關抑制劑





 





RELATED PRODUCTS:





LDN-57444 (Cat. No. 2016-5, 25)





Spautin-1 (Cat. No. 2037-5, 25)





USP14 Inhibitor, IU-1 (Cat. No. 1845-5, 25)





USP7/USP47 Inhibitor P22077 (Cat. No. 2301-5, 25)





WP-1130 (Cat. No. 2014-5, 25)





 


 


【連絡人】許虹宜 0920-312382 Hung-Yi Hsu





E-mailtaiding.biotech@msa.hinet.net





【公司電話】02-86609496【公司傳真】02-86609342





【相關網址】www.biovision.com





【公司網址】www.biopioneer.com.tw





 

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DUB相關抑制劑/IU-1 貨號#2301-5





USP14
Inhibitor, IU1


A USP14 inhibitor





IU1是一種細胞滲透性的,可逆的proteasome(蛋白酶)選擇性抑制劑,作用於USP14 時,IC504.7 μ M,比作用於IsoT 選擇性高25倍。





Alternate
Name/Synonyms:
1-[1-(4-fluorophenyl)-2,5-dimethyl-1H-pyrrol-3-yl]-2-(1-pyrrolidinyl)-ethanone





Appearance:
White
to off-white solid





CAS
Number: 
314245-33-5





Molecular
Formula: 
C₁₈H₂₁FNO





Molecular
Weight:
300.37





Purity:
≥98%
by HPLC





Solubility:
DMSO
(20 mg/ml)





Storage
Temp.:
-20°C





Shipping
Conditions:
gel pack





Description:
A
cell-permeable, reversible and selective inhibitor of the deubiquitinating
activity of human USP14 (IC
₅₀ = 4-5 µM). IU1 promotes
cell survival during proteotoxic stress and selectively stimulates
ubiquitin-dependent protein degradation in vitro at 34 μM. 





Handling:
Protect
from air and light


 


 





--其它DUB抑制劑--





RP-619  貨號#2302-5





http://tw.myblog.yahoo.com/taiding2000/article?mid=1162&prev=1163&next=1156





LDN-57444 貨號#2016-5





http://tw.myblog.yahoo.com/taiding2000/article?mid=1163&prev=1164&next=1162
 





P22077 貨號#2301-5





http://tw.myblog.yahoo.com/taiding2000/article?mid=1164&prev=1165&next=1163





IU-1 貨號#2301-5





http://tw.myblog.yahoo.com/taiding2000/article?mid=1166&prev=1167&next=1165





WP-1130 貨號#2014-5





http://tw.myblog.yahoo.com/taiding2000/article?mid=1167&prev=1168&next=1166





Spautin-1 貨號#2037-5





http://tw.myblog.yahoo.com/taiding2000/article?mid=1168&prev=-1&next=1167





DUB相關抑制劑





 





RELATED PRODUCTS:





LDN-57444 (Cat. No. 2016-5, 25)





Spautin-1 (Cat. No. 2037-5, 25)





USP14 Inhibitor, IU-1 (Cat. No. 1845-5, 25)





USP7/USP47 Inhibitor P22077 (Cat. No. 2301-5, 25)





WP-1130 (Cat. No. 2014-5, 25)





 


 





【連絡人】許虹宜 0920-312382 Hung-Yi Hsu





E-mailtaiding.biotech@msa.hinet.net





【公司電話】02-86609496【公司傳真】02-86609342





【相關網址】www.biovision.com





【公司網址】www.biopioneer.com.tw





 


 

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DUB相關抑制劑/IU-1 貨號#2301-5





USP14
Inhibitor, IU1


A USP14 inhibitor





IU1是一種細胞滲透性的,可逆的proteasome(蛋白酶)選擇性抑制劑,作用於USP14 時,IC504.7 μ M,比作用於IsoT 選擇性高25倍。





Alternate
Name/Synonyms:
1-[1-(4-fluorophenyl)-2,5-dimethyl-1H-pyrrol-3-yl]-2-(1-pyrrolidinyl)-ethanone





Appearance:
White
to off-white solid





CAS
Number: 
314245-33-5





Molecular
Formula: 
C₁₈H₂₁FNO





Molecular
Weight:
300.37





Purity:
≥98%
by HPLC





Solubility:
DMSO
(20 mg/ml)





Storage
Temp.:
-20°C





Shipping
Conditions:
gel pack





Description:
A
cell-permeable, reversible and selective inhibitor of the deubiquitinating
activity of human USP14 (IC
₅₀ = 4-5 µM). IU1 promotes
cell survival during proteotoxic stress and selectively stimulates
ubiquitin-dependent protein degradation in vitro at 34 μM. 





Handling:
Protect
from air and light





【連絡人】許虹宜 0920-312382 Hung-Yi Hsu





E-mailtaiding.biotech@msa.hinet.net





【公司電話】02-86609496【公司傳真】02-86609342





【相關網址】www.biovision.com





【公司網址】www.biopioneer.com.tw





 


 

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DUB相關抑制劑/ P22077 貨號#2301-5





USP7/USP47
Inhibitor, P 22077







P22077 ubiquitin-specific
protease USP7
(泛素特異性蛋白酶USP7抑制劑,EC508.6 μM, 也抑制緊密相關的去泛素化酶(DUB) USP47





A USP7/USP47 inhibitor





Product Name: USP7/USP47
Inhibitor, P 22077





Alternate Name/Synonyms: 1-(5-(2,4-Difluorophenylthio)-4-nitrothiophen-2-yl)ethanone;
DUB inhibitor





Description: A cell-permeable inhibitor of the
ubiquitin-specific protease (IC
₅₀ = 8.6 µM) and
the closely related deubiquitinase (DUB) USP47. P22077 displays weak or no
inhibition (EC
₅₀>50 µM) towards a panel of 14
isopeptidases and other proteases. Inhibits USP7-mediated p53 deubiquitination.





Peptide Sequence: No





Appearance: Yellow solid





Formulation: N/A





CAS Number: 1247819-59-5





Molecular Formula: C12H7F2NO3S





Molecular Weight: 315.32





Purity: ≥98% by HPLC





Solubility: DMSO (~ 30 mg/ml)





Storage Temp.: -20°C





Shipping Conditions: gel pack





Handling: Protect from air and light


 







--其它DUB抑制劑--





RP-619  貨號#2302-5





http://tw.myblog.yahoo.com/taiding2000/article?mid=1162&prev=1163&next=1156





LDN-57444 貨號#2016-5





http://tw.myblog.yahoo.com/taiding2000/article?mid=1163&prev=1164&next=1162
 





P22077 貨號#2301-5





http://tw.myblog.yahoo.com/taiding2000/article?mid=1164&prev=1165&next=1163





IU-1 貨號#2301-5





http://tw.myblog.yahoo.com/taiding2000/article?mid=1166&prev=1167&next=1165





WP-1130 貨號#2014-5





http://tw.myblog.yahoo.com/taiding2000/article?mid=1167&prev=1168&next=1166





Spautin-1 貨號#2037-5





http://tw.myblog.yahoo.com/taiding2000/article?mid=1168&prev=-1&next=1167





DUB相關抑制劑





 





RELATED PRODUCTS:





LDN-57444 (Cat. No. 2016-5, 25)





Spautin-1 (Cat. No. 2037-5, 25)





USP14 Inhibitor, IU-1 (Cat. No. 1845-5, 25)





USP7/USP47 Inhibitor P22077 (Cat. No. 2301-5, 25)





WP-1130 (Cat. No. 2014-5, 25)





 


 


【連絡人】許虹宜 0920-312382 Hung-Yi Hsu





E-mailtaiding.biotech@msa.hinet.net





【公司電話】02-86609496【公司傳真】02-86609342





【相關網址】www.biovision.com





【公司網址】www.biopioneer.com.tw





 


 

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DUB相關抑制劑/ LDN-57444 貨號#2016-5





LDN-57444

A selective UCHL1 inhibitor





LDN-57444是可逆的DUB競爭性抑制劑,抑制UCch-L1時,IC500.88 μM,比作用於亞型 Uch-L3選擇性高30倍。





Product Name: LDN-57444





Alternate Name/Synonyms: 3-(O-acetyloxime),
5-chloro-1-[(2,5-dichlorophenyl)methyl]-1H-Indole-2,3-dione, C30, LDN 57444





Description: Cell-permeable. LDN-57444 is a potent,
reversible, competitive and active site-directed inhibitor of UCHL1 (Ki = 0.4
μM). Decreases proteasome activity and increases levels of ubiquitinated
proteins. Induces apoptosis. Causes dramatic alterations in synaptic protein
distribution and spine morphology in vivo.





Peptide Sequence: N/A





Appearance: Yellow solid





Formulation: N/A





CAS Number: 668467-91-2





Molecular Formula: C17H11Cl3N2O3





Molecular Weight: 397.64





Purity: ≥98% by TLC





Solubility: DMSO (20 mg/ml)





Storage Temp.: -20°C





Shipping Conditions: Gel pack





Handling: Protect from air and light


 







--其它DUB抑制劑--





RP-619  貨號#2302-5





http://tw.myblog.yahoo.com/taiding2000/article?mid=1162&prev=1163&next=1156





LDN-57444 貨號#2016-5





http://tw.myblog.yahoo.com/taiding2000/article?mid=1163&prev=1164&next=1162
 





P22077 貨號#2301-5





http://tw.myblog.yahoo.com/taiding2000/article?mid=1164&prev=1165&next=1163





IU-1 貨號#2301-5





http://tw.myblog.yahoo.com/taiding2000/article?mid=1166&prev=1167&next=1165





WP-1130 貨號#2014-5





http://tw.myblog.yahoo.com/taiding2000/article?mid=1167&prev=1168&next=1166





Spautin-1 貨號#2037-5





http://tw.myblog.yahoo.com/taiding2000/article?mid=1168&prev=-1&next=1167





DUB相關抑制劑





 





RELATED PRODUCTS:





LDN-57444 (Cat. No. 2016-5, 25)





Spautin-1 (Cat. No. 2037-5, 25)





USP14 Inhibitor, IU-1 (Cat. No. 1845-5, 25)





USP7/USP47 Inhibitor P22077 (Cat. No. 2301-5, 25)





WP-1130 (Cat. No. 2014-5, 25)





 


 


【連絡人】許虹宜 0920-312382 Hung-Yi Hsu





E-mailtaiding.biotech@msa.hinet.net





【公司電話】02-86609496【公司傳真】02-86609342





【相關網址】www.biovision.com





【公司網址】www.biopioneer.com.tw





 

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DUB抑制劑/RP-619  貨號#2302-5





DUB
Inhibitor, PR-619


A deubiquitinylating enzymes (DUB) inhibitor





Product Name: DUB Inhibitor,
PR-619





DUB抑制劑。PR-619是非選擇性的,可逆的 deubiquitinylating enzymes (DUBs)(去泛素化 )抑制劑,EC501-20
μM





Alternate Name/Synonyms: 2,6-Diaminopyridine-3,5-bis(thiocyanate),
3,5-Dithiocyanatopyridine-2,6-diamine





Description: A cell-permeable, reversible, and broad-spectrum
inhibitor of the deubiquitinylating enzymes (DUBs). Treatment of cells with
PR-619 results in the accumulation of both ubiquitin and sumo-conjugated
proteins.





Peptide Sequence: No





Appearance: Yellow solid





Formulation: N/A





CAS Number: 2645-32-1





Molecular Formula: C7H5N5S2





Molecular Weight: 223.28





Purity: ≥98% by HPLC





Solubility: DMSO (~ 5 mg/ml)





Storage Temp.: -20°C





Shipping Conditions: gel pack





Handling: Protect from air and light


 





--其它DUB抑制劑--





RP-619  貨號#2302-5





http://tw.myblog.yahoo.com/taiding2000/article?mid=1162&prev=1163&next=1156





LDN-57444 貨號#2016-5





http://tw.myblog.yahoo.com/taiding2000/article?mid=1163&prev=1164&next=1162
 





P22077 貨號#2301-5





http://tw.myblog.yahoo.com/taiding2000/article?mid=1164&prev=1165&next=1163





IU-1 貨號#2301-5





http://tw.myblog.yahoo.com/taiding2000/article?mid=1166&prev=1167&next=1165





WP-1130 貨號#2014-5





http://tw.myblog.yahoo.com/taiding2000/article?mid=1167&prev=1168&next=1166





Spautin-1 貨號#2037-5





http://tw.myblog.yahoo.com/taiding2000/article?mid=1168&prev=-1&next=1167





DUB相關抑制劑





 





RELATED PRODUCTS:





LDN-57444 (Cat. No. 2016-5, 25)





Spautin-1 (Cat. No. 2037-5, 25)





USP14 Inhibitor, IU-1 (Cat. No. 1845-5, 25)





USP7/USP47 Inhibitor P22077 (Cat. No. 2301-5, 25)





WP-1130 (Cat. No. 2014-5, 25)




 


 


【連絡人】許虹宜 0920-312382 Hung-Yi Hsu



E-mailtaiding.biotech@msa.hinet.net



【公司電話】02-86609496【公司傳真】02-86609342



【相關網址】www.biovision.com



【公司網址】www.biopioneer.com.tw


 


 


 


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癌化特性之分析套組-1




 Cell
Proliferation Assay----
細胞增殖分析





癌細胞之生長速度比正常細胞快,藉由測定細胞繁殖的速度,可作為判斷細胞癌化的條件。細胞增殖分析中,常見的分析方式包括WST-1WST-8 及測量細胞內ATP的含量





WST-8分析試劑
(呈色分析) 資料




http://tw.myblog.yahoo.com/taiding2000/article?mid=972&prev=973&next=964&l=f&fid=30





WST-1分析套組 (呈色/螢光分析)資料



http://tw.myblog.yahoo.com/taiding2000/article?mid=101&prev=102&next=91&l=f&fid=30 





ATP分析套組 (冷光分析)資料




http://tw.myblog.yahoo.com/taiding2000/article?mid=171&prev=218&next=154&l=f&fid=30  









癌化特性之分析套組-2




Angiogenesis/
Tube formation---
血管新生/血管形成





癌化過程中具有血管新生之過程,藉以提供癌細胞養份及氧氣血管新生作用是由內皮細胞所引導的過程中細胞會分泌酵素來分解基底膜造成細胞之遊走經由一連串的增生分化後最後形成管狀般的血管構造





Tube formation分析套組 (顯微鏡觀察)資料




http://tw.myblog.yahoo.com/taiding2000/article?mid=1047





活體血管新生分析套組 (定量/顯微鏡)資料




http://tw.myblog.yahoo.com/taiding2000/article?mid=1049&prev=-1&next=1047






 


癌化特性之分析套組-3




Tumor marker/
Oncomir





腫瘤組織會特異性的表現一些蛋白或 microRNA在此統統稱這群蛋白/ microRNA Tumor marker (腫瘤標記) OncomirTumor marker Oncomir 的表現量與腫瘤惡化程度成正相關





miRNA (QPCR/WB)資料








 


 癌化特性之分析套組-4




Cell
Migration Assay/ Cell Invasion Assay





Cell migration 為細胞移動之情形不只癌化細胞移動能力會提受到趨化作用之細胞的移動現象也會增加Cell invasion 為細胞分泌酵素把 ECM 降解突破基底膜的限制





Invasion/migration資料




http://tw.myblog.yahoo.com/taiding2000/article?mid=80&prev=81&next=78&l=f&fid=26











癌化特性之分析套組-5




Soft agar assay/ Colony formation assay





正常的細胞必需貼附基底生長; 癌化傾向的細胞可在半固狀





的凝膠 (Soft
agar)
中懸浮著生長形成 Colony


 


 





【連絡人】許虹宜 0920-312382



E-mailtaiding.biotech@msa.hinet.net



【臺北公司】02-86609496【傳真】02-86609342



【相關網址】www.biopioneer.com.tw


 

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癌化特性之分析套組-microRNA表現分析








腫瘤組織會特異性的表現一些蛋白或 微型核糖核酸(microRNA)在此統統稱這群蛋白/ microRNA Tumor marker (腫瘤標記) OncomirTumor marker Oncomir 的表現量與腫瘤惡化程度成正相關




microRNA 是一種non-coding
RNA (
不會轉譯出蛋白質的RNA),在生物體內non-coding RNA扮演了調控基因的表現、蛋白質活性、訊息傳遞、等重要角色。MicroRNA是一群非常短的RNA,大約只有二十幾個鹼基組成,MicroRNA雖短,但卻具有影響基因表現的能力。在生化機制或是功能上,miRNAsiRNA是相當類似的。



ABMgood提供各種microRNA表現與偵測的產品;其種類包括有miRNA表現載體、miRNA分離與偵測套組,另提供更多研究miRNA的資料來源。


 


miRNA表現載體





 


    GFP miRNA
Lentivirus/Vector





    miRNA Lentivirus/Vector





    iCumate
miRNA Lentivectors





    miRNA(microRNA) Adenovirus





    miRNA
Inhibitor Lentivirus/Vector





    miRNA
Inhibitor Adenovirus





    3'UTR
miRNA Reporter & Cell Line





 


miRNA萃取與偵測





 


qPCR miRNA Profiling Kits





miRNA Primers





miRNA qPCR Mastermixes





miRNA
cDNA Synthesis Kits





【連絡人】許虹宜 0920-312382



E-mailtaiding.biotech@msa.hinet.net



【臺北公司】02-86609496【傳真】02-86609342



【相關網址】www.biopioneer.com.tw



【原廠網址】www.abmgood.com


 

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癌化特性之分析套組-Angiogenesis Assay





活體新血管試驗套組DIVAA,是市面上唯一可以定量且具重覆性的活體內血管新生套組。取代傳統Plug assay自體吸收問題。此套組利用血管新生試驗管(angioreactor),
將待測藥物與BME混合物填至試驗管,並稙入老鼠體內進行血管新生試驗。與傳統的Matrigel plug方法相比;BME混合物不會被自體吸收,因此,除了定性外亦可同時定量血管新生的程度,試驗結果再現性高、人為誤差程度小….是做活體血管新生的最佳選擇。





Cultrex®
DIVAA Starter Kit




































Catalog
#




Product
Name




Size



 



3450-048-SK




Cultrex®
DIVAA Starter Kit




48
tests



 


 



Cultrex® DIVAA Starter Kit

Catalog #:
3450-048-SK



 







Cultrex®
Directed In Vivo Angiogenesis Assay (DIVAA)


Includes:

FGF/VEGF & AngioRack


DIVAA is the first in vivo system for the study of
angiogenesis that provides quantitative and reproducible results.1 During the
course of the assay, implant grade silicone cylinders closed at one end,
called angioreactors, are filled with 20 μl of Trevigen's PathClear® basement
membrane extract (BME) premixed with or without angiogenic-modulating
factors*. These angioreactors are then implanted subcutaneously in the dorsal
flank of nude mice. Accompanied with the onset of angiogenesis, vascular
endothelial cells proceed to grow into the BME and form vessels in the
angioreactor. As early as nine days post-implantation, there are enough cells
to determine an effective dose response to angiogenic modulating factors. The
sleek design of the angioreactor provides a standardized platform for
reproducible and quantifiable in vivo angiogenesis assays. Compared to the
plug assay5, the angioreactor prevents assay errors due to absorption of the
basement membrane extract by the mouse. In addition, the angioreactor uses
only a fraction of the materials conserving both BME and test compounds used,
and up to four angioreactors may be implanted in each mouse, allowing for
greater statistical power. Trevigen’s DIVAA has been used in a variety of
investigations.1-4, 6-9.



DIVAA
is available in three formats:


DIVAA
Starter Kit Catalog # 3450-048-SK


The DIVAA Starter Kit was designed to introduce the technology
and give the user practical

experience assessing angiogenesis. It contains 48 Angioreactors, enough
growth factor to

induce angiogenesis all 48 angioreactors, and an Angiorack™ designed to hold
the

Angioreactors during the course of assay setup.



DIVAA
Activation Kit Catalog # 3450-048-K


The DIVAA Activation Kit was designed for assessing
angiogenesis activation. It contains 48

angioreactors and enough growth factor for eight positive controls.



DIVAA
Inhibition Assay Catalog # 3450-048-IK


The DIVAA Inhibition Kit was designed for assessing
angiogenesis inhibition. It contains 48

Angioreactors and enough growth factor to induce angiogenesis in all 48
angio-reactors.

* The PathClear® designation means:



§   
No bacterial or fungal growth detected after incubation at 37°C for 14 days following
USP XXIV Chapter 71 sterility test.



§   
Negative by PCR test for: mycoplasma, 17 bacterial and virus
strains typically included in mouse antibody production (MAP) testing, plus
13 additional murine infectious agents including LDEV, for a total of 31
organisms and viruses.



REFERENCES:


1.Guedez L, Rivera AM, Salloum R, Miller ML, Diegmueller JJ,
Bungay PM, Stetler-Stevenson WG. 2003. Quantitative Assessment of Angiogenic
Response by the Directed In Vivo Angiogenesis Assay. AJP 162:1431-1439.

2. Seo D, Li H, Guedez L, Wingfield PT, Diaz T, Salloum R, Wei B,
Stetler-Stevenson WG. 2003. TIMP-2 Mediated Inhibition of Angiogenesis: An
MMP-Independent Mechanism. Cell 114:171-180.

3. Martinez A, Vos M, Guedez L, Kaur G, Chen Z, Garayoa M, Pio R, Moody T,
Stetler- Stevenson WG, Kleinman HK, Cuttitta F. 2002. The effects of
adrenomedullin overexpression in breast tumor cells. J Natl Cancer Inst
94:1226-37.

4. Wang T, Ward Y, Tian L, Lake R, Guedez L, Stetler-Stevenson WG, Kelly K.
2005. CD97, an adhesion receptor on inflammatory cells, stimulates
angiogenesis through binding integrin counterreceptors on endothelial cells.
Blood 105:2836-44.

5. Lee MS, Moon EJ, Lee SW, Kim MS, Kim KW, Kim YJ. 2001. Angiogenic activity
of pyruvic acid in in vivo and in vitro angiogenesis models. Cancer Res
61:3290-3.

6. Kurozumi K, Hardcastle J, Thakur R, Shroll J, Nowicki M, Otsuki A, Chiocca
EA, Kaur B. 2008. Oncolytic HSV-1 infection of tumors induces angiogenesis
and upregulates CYR61. Mol Ther 16:1382-91.

7. Basile JR, Holmbeck K, Bugge TH, Gutkind JS. 2007. MT1-MMP controls
tumorinduced angiogenesis through the release of semaphoring 4D. J Biol Chem
282:6899-905.

8. Liu S, Wang H, Currie BM, Molinolo A, Leung HJ, Moayeri M, Basile JR,
Alfano RW, Gutkind JS, Frankel AE, Bugge TH, Leppla SH. 2008.
Matrix-metalloproteinase-activated anthrax lethal toxin demonstrates high
potency in targeting tumor vasculature. J Biol Chem 283:529-40.

9. Sakamoto K, Maeda S, Hikiba Y, Nakagawa H, Hayakawa Y, Shibata W, Yanai A,
Ogura K, Omata M. 2009. Constitutive NF-κB activation in colorectal carcinoma
plays a key role in angiogenesis, promoting tumor growth. Clin Cancer Res
15:2248-58.

10. U.S.
Patent 4,829,000

11. U.S.
Patent 5,158,874







Kit
Components:








































Catalog #




Component / Size




3450-048-01




DIVAA Angioreactor / 48 reactors




3450-048-02




DIVAA BME / 6 x 200uL




3450-048-03




DIVAA Wash Buffer / 25 ml




3450-048-04




DIVAA FGF-2 Positive Control / 100 ng/10 uL




3450-048-05




DIVAA Cell Sperse / 15 ml




3450-048-08




Heparin Solution / 10 µl 2mg/ml




3450-048-09




AngioRack / 48 positions




3450-048-B10




DIVAA FGF-2 (1.8 ng)/VEGF(600 ng) / 60 µl









其它Angiogenesis相關產品





Cultrex® Angiogenesis Products















































Catalog
#




Product
Name




Size




3450-048-DA




Cultrex®
DIVAA Angioreactor and BME




48
Wells




3450-048-FL




Cultrex®
DIVAA FITC-Lectin & Buffer




48
Wells




3450-048-IK




Cultrex®
DIVAA Inhibition Kit




48
Wells




3450-048-K




Cultrex®
DIVAA Activation Kit




48
Wells




3450-048-SK




Cultrex®
DIVAA Starter Kit




48
tests




3470-096-K




Cultrex®
In Vitro Angiogenesis Assay Tube Formation Kit




96
Wells




3471-096-K




Cultrex®
In Vitro Angiogenesis Assay Endothelial Cell Invasion Kit




96
tests






【連絡人】許虹宜 0920-312382



E-mailtaiding.biotech@msa.hinet.net



【臺北公司】02-86609496【傳真】02-86609342



【相關網址】www.biopioneer.com.tw


 


【原廠網址】www.trevigen.com


 

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ATPase分析套組---產品介紹





產品應用ATPases酵素活性之分析



























內附有ATPase inhibitorATPase酵素活性分析,採用超高靈敏度Malachite green磷酸根呈色方法,克服傳統呈色方法,低靈敏度限制。測量目標樣本中,
ATPases
酵素活性。





ATPase活性分析檢測





產品應用





Innova Biosciences 提供之ATPase分析套組,可探討酵素活性是否扮演transportsignal transduction,
protein biosynthesis
cell differentiation之角色。應用於ATPase活性分析。提供high-throughput screen 大量ATPase inhibitor。可建立快速藥物篩選系統。





ATPase活性分析檢測





產品特點





1.     
提供high-throughput screen 大量ATPase inhibitor





2.     
ATPase酵素分析套組,包括stabilizing solution,可避開acid labile substrate所造成的高背景值,其背景值很低(
1mM )





2. 其分析波段為600~660nm,避開一般colored compounds之干擾。





3. Pi-dye複合物極為穩定,其訊號強度可維持數小時之久。





4. Superior呈色系統。採用Malachite Green磷酸根呈色方式,呈色靈敏度比傳統呈色方法高,克服低靈敏度的限制。





5. 適用於大部份的assay buffer





6. 蛋白質濃度高時,不會抑制顏色的形成。





 





訂購貨號# 601-0120,
196 assays
(靈敏度4~40uM)





訂購貨號# 601-0121,
480 assays
(靈敏度4~40uM)





訂購貨號# 601-0122,
480*5 assays
(靈敏度4~40uM)





產品內容物





 





1 x 10ml of PiColorLock™ Gold* (1 x 25ml)





1 x 0.25ml of Accelerator (1 x 0.5ml)





1 x 5ml of Stabiliser (1 x 10ml)





1 x 1.5ml of 0.1M MgCl2 (2 x 1.5ml)





1 x 5ml of 0.5M Tris pH 7.5 (1 x 10ml)





1 x 5ml of 0.1mM Pi standard (1 x 10ml)





更多參考內容:





原廠連結: http://www.innovabiosciences.com/







相關商品





PiColorLock磷酸根分析檢測套組


http://tw.myblog.yahoo.com/taiding2000/article?mid=723&prev=724&next=715&l=f&fid=26


GTPase活性分析檢測套組


http://tw.myblog.yahoo.com/taiding2000/article?mid=724&prev=725&next=723&l=f&fid=26


 


游離磷酸根移除resin


5g
(501-0015)


Inorganic phosphate (Pi) is a
product of many enzymatic reactions, for example, those catalysed by
phosphatases and ATPases. Measurement of released Pi allows enzyme activity to
be determined, but contamination of enzymes or buffers by Pi can lead to unacceptably
high assay backgrounds.


PiBind™ resin provides a quick
and easy way to remove contaminating Pi from buffers. The resin works over a
broad range of pH values and is unaffected by many commonly used buffer
additives. PiBind™ resin can also be used to remove Pi from protein samples
(e.g. tissue extracts). This method is quicker than dialysis and unlike
desalting does not necessarily lead to a significant dilution of the sample.





ATPase酵素活性分析套組---文獻發表





ATPase酵素活性分析套組





ATPase Assay Kit





View
Product








 





【供應廠商】太鼎生物科技有限公司





【連絡人】許虹宜0920-312382





【連絡信箱】taiding.biotech@msa.hinet.net





【臺北公司】02-86609496【台中公司】0935-229803
陳俊豪





【公司網址】 www.biopioneer.com.tw





 


 


 

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PiColorLock磷酸根呈色----產品介紹

產品應用於ATPasesphosphatasesGTPasesnucleotidases酵素活性之分析。

 

PiColorLock採用超高靈敏度Malachite green磷酸根呈色方法,克服傳統呈色方法,低靈敏度限制。測量目標樣本中,是否含有ATPasesphosphatasesGTPasesnucleotidases酵素活性。

 

inorganic phosphate detection

產品應用

Innova Biosciences PiColorLock 針對實驗初篩所設計,可探討酵素活性是否扮演transportsignal transduction, protein biosynthesiscell differentiation之角色。應用於ATPaseGTPaseProteinases活性分析。提供high-throughput screen 大量ATPaseGTPase inhibitor。可建立快速藥物篩選系統。

 

inorganic phosphate detection

產品特點

  1. PiColorLock分析套組,包括stabilizing solution,可避開acid labile substrate所造成的高背景值,其背景值很低(1mM )
  2. 其分析波段為600~660nm,避開一般colored compounds之干擾。
  3. Pi-dye複合物極為穩定,其訊號強度可維持數小時之久。
  4. Superior呈色系統。採用Malachite Green磷酸根呈色方式,呈色靈敏度比傳統呈色方法高,克服低靈敏度的限制。
  5. 適用於大部份的assay buffer
  6. 蛋白質濃度高時,不會抑制顏色的形成。

 

Pi Colorlock (ALS)(靈敏度20~200uM)

PiColorLock產品,有分為Gold (靈敏度4~40uM)ALS(靈敏度20~200uM)

訂購貨號# 302-0125, 625/1560 assays (靈敏度20~200uM)

訂購貨號# 302-0500, 2500/6250 assays (靈敏度20~200uM)

PiColorLock is a phosphate detection reagent for measuring Phosphatases, ATPases and Nucleotidases.

 

Pi Colorlock (Gold)(靈敏度4~40uM)

訂購貨號# 303-0125, 625/1560 assays (靈敏度4~40uM)

訂購貨號# 303-0500, 2500/6250 assays (靈敏度4~40uM)

PiColorLock is a phosphate detection reagent for measuring Phosphatases, ATPases and Nucleotidases.

 

Colorimetric assays for ATPases are invariably based on the formation of colored complexes between an inorganic phosphate and a dye molecule under acidic conditions. Such assays are beset with problems of reagent precipitation and high backgrounds caused by impure substrates (i.e. contaminated with inorganic phosphate) and/or by non-enzymatic (acid) hydrolysis of the substrate.

At the heart of our assay kits is PiColorLock Gold, a superior phosphate detection reagent which ensures high stability of the colored dye-phosphate complexes. Together with specially purified substrates and proprietary stabilizers our kits offer the lowest possible assay backgrounds and outstanding assay performance.

Uniquely, Innova Biosciences ATPase assay kits contain highly purified ATP, which have had any free inorganic phosphate removed – this means the assays have the lowest possible background signal.

PiColorLock reagent may also be purchased separately for developing simple assays for any phosphate-generating enzyme. It is offered in both high (Gold) and standard sensitivity (ALS) formulations to suit almost any assay situation.

 

更多參考內容:

原廠連結: http://www.innovabiosciences.com/

 

相關商品

ATPase活性分析檢測套組

http://tw.myblog.yahoo.com/taiding2000/article?mid=725&prev=-1&next=724

GTPase活性分析檢測套組

http://tw.myblog.yahoo.com/taiding2000/article?mid=724&prev=725&next=723&l=f&fid=26

磷酸根移除resin

5g (501-0015)

Inorganic phosphate (Pi) is a product of many enzymatic reactions, for example, those catalysed by phosphatases and ATPases. Measurement of released Pi allows enzyme activity to be determined, but contamination of enzymes or buffers by Pi can lead to unacceptably high assay backgrounds.

PiBind™ resin provides a quick and easy way to remove contaminating Pi from buffers. The resin works over a broad range of pH values and is unaffected by many commonly used buffer additives. PiBind™ resin can also be used to remove Pi from protein samples (e.g. tissue extracts). This method is quicker than dialysis and unlike desalting does not necessarily lead to a significant dilution of the sample.

 

PiColorlock Gold/ALS文獻發表

PiColorlock Gold

View

Product

 

PiColorlock ALS

View Product

 

 

PiBind resin

View Product

 

 

【供應廠商】太鼎生物科技有限公司

【連絡人】許虹宜0920-312382

【連絡信箱】taiding.biotech@msa.hinet.net

【臺北公司】02-86609496

【公司網址】 www.biopioneer.com.tw

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食物藥物殘留(Drug Residue)




提供項目廣泛的食品飼料安全檢測,利用簡單、快速、靈敏且經濟的酵素免疫篩檢方法(ELISA)以及快速檢測片,使政府法規單位、食品製造處理商、以及食物品保及標準機構可以檢測肉品、牛奶、魚、蝦等食品中藥物殘留濃度。




 





















































































































































藥物分類




檢測藥物




編號




Anabolic steroids




Trenbolone 群勃龍




TB2106




19-nortestosterone 去甲睾酮




NT2105




Boldenone 勃地酮




BD2382




Stanozolol 康力龍




SW2418




Anti-microbial




Sulphaquinoxaline 磺胺喹惡啉




SQ2145




Sulphadiazine 磺胺嘧啶




SZ2147




Sulphamethazine 磺胺二甲嘧啶




SM2146




Sulphamethoxazole 磺胺甲噁唑




SZ3471




Chloramphenicol 氯黴素




CN1469




β-Lactams β-內醯胺類




BL3448




β-Lactams Standard
Accessory Kit




BL1371




Quinolones 諾酮類




QL3454




Flumequine 氟甲喹




FQ3460




Nitrofurans Accessory 硝基呋喃類




NF3464




- AOZ




NF3465




- AMOZ




NF3462




- AHD (plate)




NF3463




- SEM (plate)




NF3461




Tetracyclines (generic) 四環素 Currently in development




 




Streptomycin / Dihydrostreptomycin 鏈黴素 / 雙氫鏈黴素




STP3468




Enrofloxacin and Ciprofloxacin 恩諾沙星和環丙沙星




EC3473




b-agonists




Clenbuterol 克侖特羅/brombuterol




CB1418




b-agonists 受體素(clenbuterol, salbutamol 沙丁胺醇, mabuterol 馬布特羅, brombuterol 波洛母特羅, mapenterol, terbutaline,
perbuterol, methyclenbuterol, carbuterol
卡布比妥)




SU2148




Ractopamine 萊克多巴胺 ( 培林 又名瘦肉精 )




RT3451




Drugs of Abuse




Ketmine 氯胺酮 (specific for
norketamine)




KT3459




Tri-phenylmethane dyes




Leucomalachite Green 還原型孔雀綠




LMG3466




Non Steroidal



Anti-inflammatory




Corticosteroids 皮質類固醇(dexamethasone,
flumethasone, betamethasone)




DM2156




Flunixin 氟尼辛




FL3458




Phenylbutazone 苯基丁氮酮




PB3456




Oestrogenic hormones




Stilbenes 二苯乙烯類(diethylstilbestrol,
hexoestrol, dienoestrol)




SJ2152




Zeranol 玉米赤黴醇




ZR2421




Urine Control




Clenbuterol 克侖特羅




CB3449







 



ELISA Test Kits 檢測試劑:敏感度高/使用容易方便/成本低/ 快速







Melamine 三聚氰胺
Dioxin/Furan 戴奧辛

Green Spring ELISA Test Kits 檢測試劑:敏感度高/使用容易方便/成本低/ 快速




















Food Safety ELISA Test
Kits
Florfenicol 氟甲氯黴素Gentamicin 慶大黴素
Ampicillin 氨芐青黴素Olaquindox 歐來金得
Benzyl penicillin 芐青黴素Neomycine 新黴素

Tylosin 泰黴素




 



































Food Safety rapid Test
strip
SpecSensitivityDetection Time
Clenbuterol 克侖特羅10 test / kit3 ppb5 min
Chloramphenicol 氯黴素10 test / kit0.1 ppb7 min
Chloramphenicol 氯黴素10 test / kit0.3 ppb10 min
Ractopamine 萊克多巴胺10 test / kit1 ppb10 min
Ractopamine 萊克多巴胺10 test / kit1 ppb10 min

 


ELISA Test Kits
檢測試劑:
敏感度高/使用容易方便/成本低/ 快速




本公司尚有代理美國 BIOO
公司所生產的藥物殘留及毒物等檢測試劑




Dairy Testing




Industrial Contaminants




Microbiology / Hygiene




Toxins Mycotoxins / Phycotoxins / Scombrotoxin (Histamine)




Veterinary Drugs
Antibiotics / Hormones / Other Veterinary Drugs




Pesticides






【供應廠商】太鼎生物科技有限公司



【連絡人】許虹宜0920-312382



【連絡信箱】taiding.biotech@msa.hinet.net



【臺北公司】02-86609496【台中公司】0935-229803 陳俊豪



【公司網址】 www.biopioneer.com.tw

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HDAC Assay Kits & Reagents



 


HDACs牽涉轉錄調控,並誘導細胞凋亡或癌細胞之分化。Biovision提供螢光及呈色分析套組,操作簡單且快速,方便測量樣品中的HDAC活性。此方式排除放射性標誌,萃取等傳統分析。


 


Inhibition of histone deacetylases (HDACs) has been implicated to modulate transcription and to induce apoptosis or differentiation in cancer cells. The HDAC Fluorometric and Colorimetric Assay Kits provide simple procedures for fast and convenient measurement of HDAC activity by fluorometric and colorimetric methods. The methods eliminate radioactivity, extractions, or chromatography, as used in the traditional assays. The new procedures require only two easy steps, both performed on the same microtiter plate. First, the HDAC fluorometric (or colorimetric) substrate, which comprises an acetylated lysine side chain, is incubated with a sample containing HDAC activity (e.g., HeLa nuclear extract). Deacetylation sensitizes the substrate, and thus in the second step, treatment with the Lysine Developer produces a fluorophore (or chromophore), which can be analyzed using a fluorometric (or colorimetric) plate reader. The HDAC Inhibitor Drug Screening Kit provides sufficient positive controls for screening 100 samples of HDAC inhibitor candidates in drug discovery. HDAC inhibitors and antibodies are also available separately.


 


HDAC於腫瘤形成之重要性】


組蛋白的乙醯化修飾對腫瘤的發生發展起重要作用。正常細胞體一旦出現核內組蛋白乙醯化與去乙醯化的失衡,即會導致正常的細胞週期與細胞代謝行為的改變而誘發腫瘤。組蛋白去乙醯化酶(histone deacetylasesHDACs)催化組蛋白的去乙醯化,維繫組蛋白乙醯化與去乙醯化的平衡狀態,與癌相關基因轉錄表達、細胞增殖分化及細胞凋亡等諸多過程密切相關。組蛋白去乙醯化酶(histone deacetylasesHDACs)是維持染色體的基本組成單位核小體中組蛋白乙醯化平衡的關鍵酶類之一,其催化組蛋白的去乙醯化作用,與基因轉錄抑制密切相關,牽涉到促基因沉默的諸多過程,是抗腫瘤藥物設計中的熱門靶標。


 





















HDAC Assay Kits



Cat#



Size



HDAC Fluorometric Assay Kit



K330-100



100 assays



HDAC Colorimetric Assay Kit



K331-100



100 assays



HDAC Inhibitor Drug Screening Kit



K340-100



100 assays



 





















 HDAC Enzymes



Cat#



Size



HeLa Nuclear Extract



1641-01



100 ug



HeLa Nuclear Extract



1641-1



1 mg



Recombinant HAT Protein



1137-100



100 ug



 

























































 HDAC Inhibitors



Cat#



Size



Apicidin



1601-1



1 mg



M344



1607-1



1 mg



MS-275 (Entinostat, SNDX-275)



1590-1



1 mg



MS-275 (Entinostat, SNDX-275)



1590-5



5 mg



Panobinostat (LBH589)



1612-1



1 mg



Panobinostat (LBH589)



1612-5



5 mg



Sodium 4-phenylbutyrate



1608-100



100 mg



Sodium 4-phenylbutyrate



1608-1000



1 gram



Sodium butyrate



1609-1000



1 g



Splitomicin



1610-5



5 mg



Trichostatin A



1606-1



1 mg



Valproic Acid, Sodium Salt



1647-200



200 mg



 





















































































 HDAC Inhibitor Set



Cat#



Size



DiscoveryPakTM HDAC Inhibitor Set



K851-6



6 inhibitors



HDAC Antibodies



Cat#



Size



Histone Deacetylase 1 (HDAC1) pAb



3601-100



100 ug



Histone Deacetylase 2 (HDAC2) pAb



3602-100



100 ug



Histone Deacetylase 3 (HDAC3) pAb



3603-100



100 ug



Histone Deacetylase 4 (HDAC4) pAb



3604-100



100 ug



Histone Deacetylase 4 (HDAC4) pAb



3604A-100



100 ug



Histone Deacetylase 5 (HDAC5) pAb



3605-100



100 ug



Histone Deacetylase 6 (HDAC6) pAb



3606-100



100 ug



Histone Deacetylase 7 (HDAC7) pAb



3607-100



100 ug



Histone Deacetylase 8 (HDAC8) pAb



3608-100



100 ug



Histone Deacetylase 9 (HDAC9) pAb



3609-100



100 ug



Histone Deacetylase 10 (HDAC10) pAb



3610-100



100 ug



Histone Deacetylase 11 (HDAC11) pAb



3611P-100



100 ug



Histone H 2A pAb



3621-100



100 ug



Histone H2B pAb



3622-100



100 ug



Histone H3 pAb



3623-100



100 ug



Histone H4 pAb



3624-100



100 ug



HDAC Family Antibody Set



K 333-11-30



Set of 11


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組蛋白去乙醯酶3Histone Deacetylase 3)抑制劑篩選套組


 



 


HDAC3 Inhibitor Screening Kit
Catalog#: K363-100  | Size: 100 assays


 



 Kit Summary: 
• Detection method- Fluorescence (Em/Ex = 380/500 nm) 
• Sample type- Testing candidate HDAC3 inhibitors 
• Applications- Suitable for high throughput screening of HDAC3 inhibitors


Features & Benefits: 
• Simple procedure; takes ~ 60 min 
• Fast and convenient 
• Kit contains the necessary reagents for testing putative HDAC3 inhibitors


Kit Components: 
• HDAC3 Assay Buffer 
• HDAC3 Substrate 
• HDAC-3 Enzyme 
• Developer 
• Trichostatin A


Description: 
Histone deacetylases (HDACs) represent a large family of enzymes identified as key regulators of nucleosomal histone acetylation, a major event that controls eukaryotic gene transcription and are classified into three groups. They are believed to be involved in important biological activities, such as cell differentiation, proliferation, apoptosis, and senescence. In BioVision’s HDAC-3 Inhibitor screening Kit, HDAC-3 and developer will deacetylate and cleave the substrate [Arg-His-Lys-Lys(Ac)-AFC] to release the quenched fluorescent group (AFC), which can be detected at Em/Ex=380/500 nm. In presence of a HDAC-3 inhibitor, the cleavage will be inhibited. The kit provides a rapid, simple, sensitive, and reliable test, which is also suitable for high throughput screening of HDAC-3 inhibitors. Trichostatin A (TSA) is included as a control inhibitor to compare the efficacy of the test inhibitors.


Storage Conditions: 
-20°C (Upon receipt place HDAC-3 Enzyme at -80 °C for storage.)


Shipping Conditions: 
gel pack

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