太鼎生物科技/太鼎食安科技

Product name:

Zebrafish GPX1(Glutathione Peroxidase 1) ELISA Kit

Reactivity:

Zebrafish

Alternative Names:

GSHPX1; Cellular glutathione peroxidase

Assay Type:

Sandwich

Sensitivity:

7.2 pg/mL

Standard:

1000 pg/mL

Range:

15.63-1000 pg/mL

Sample Type:

Serum, plasma and other biological fluids.

Assay Length:

3.5h

Research Area:

Enzyme & Kinase;Metabolic pathway;

Test principle:

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Glutathione Peroxidase 1(GPX1). Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Glutathione Peroxidase 1(GPX1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Glutathione Peroxidase 1(GPX1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Glutathione Peroxidase 1(GPX1) in the samples is then determined by comparing the OD of the samples to the standard curve.