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目前分類:【ELISA 、EIA分析套組】 (39)

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COL2(II型膠原蛋白)ELISA試劑盒 -ELK Biotechnology貨號ELK8629

Chicken COL2(Collagen Type II) ELISA Kit

產品商品: COL2(II型膠原蛋白)ELISA試劑盒 | 貨號ELK8629 (Sandwich ELISA),偵測的靈敏度0.32-20 ng/mL、最低靈敏度20 ng/mLELISA原理為利用抗體與抗原之間的高度專一性,做為定量標誌的方法;無論測量抗原或測量抗體都有很高的靈敏度跟專一性。該檢測方法對檢測小鼠 TNFa (腫瘤壞死因子-α)具有高靈敏度和出色的特異性ELISA商品的型式多元, 提供三明治ELISA、競爭型ELISA。可應用於serum, plasma, tissue homogenates, cell lysates, cell culture。並在 450nm ± 10nm 的波長下用分光光度計測量顏色變化。然後通過將樣品的 OD 與標準曲線進行比較來確定樣品中COL2(II型膠原蛋白)ELISA試劑盒的濃度。

Product name:

Chicken COL2(Collagen Type II) ELISA Kit
COL2(II型膠原蛋白)ELISA試劑盒

Reactivity:

Chicken

Alternative Names:

Collagen Type 2

Assay Type:

Sandwich

Sensitivity:

0.131 ng/mL

Standard:

20 ng/mL

Range:

0.32-20 ng/mL

Sample Type:

serum, plasma, tissue homogenates and other biological fluids

Assay Length:

3.5h

Research Area:

Metabolic pathway;Developmental science;Hepatology;Bone metabolism;

Test principle:

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Collagen Type II(COL2). Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Collagen Type II(COL2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Collagen Type II(COL2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Collagen Type II(COL2) in the samples is then determined by comparing the OD of the samples to the standard curve.

 

Concentration (ng/mL)

OD

Corrected OD

20.00

2.266

2.177

10.00

1.652

1.563

5.00

1.223

1.134

2.50

0.958

0.869

1.25

0.617

0.528

0.63

0.393

0.304

0.32

0.135

0.046

0.00

0.089

0.000

技術支援篇-300x105.jpg

ELK Biotechnology公司是一家專注於生命科學、免疫研究的高科技生物企業,位於Biolake, Donghu New & High Technology Development Zone, Wuhan city.ELK Biotechnology是一家專注於免疫產品和分子生物學產品研發和生產的高科技生物公司。產品涵蓋ELISA試劑盒、抗體、基因組提取試劑、分子生物學試劑。涉及免疫化學、腫瘤研究、神經生物學、細胞週期、信號轉導等領域。我們的產品有嚴格的研發和質量檢測流程。每個產品都有完整的研發、生產和質檢記錄。經驗豐富的研發人員,完善的質檢支持部門,嚴格的檢測流程,為客戶提供質量可靠的科研產品。
 
產品貨號
品項
貨號ELK1163
 TSH(促甲狀腺激素)檢測試劑盒 -ELK Biotechnology貨號ELK1163
貨號ELK8863
斑馬魚 MDA(丙二醛)檢測試劑盒 -ELK Biotechnology
貨號ELK8860
斑馬魚 CATCatalase過氧化氫酶)檢測試劑盒 -ELK Biotechnology貨號ELK8860
貨號ELK8860
斑馬魚 CATCatalase過氧化氫酶)檢測試劑盒 -ELK Biotechnology貨號ELK8860
貨號ELK8861
斑馬魚GSH(穀胱甘肽)檢測試劑盒 -ELK Biotechnology貨號ELK8861
貨號ELK8862
斑馬魚GPX1(穀胱甘肽過氧化物酶 1)檢測試劑盒 -ELK Biotechnology貨號ELK8862
貨號ELK1157
小鼠IL6(白細胞介素 6)檢測試劑盒 | ELK Biotechnology貨號ELK1157
貨號ELK1395
小鼠 TNFa (腫瘤壞死因子-α) 檢測試劑盒 | ELK Biotechnology貨號ELK1395
 
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激活素 A (ACVA)  檢測試劑盒 (酶聯免疫吸附試驗法) | CLOUD CLONE貨號CEA001Mu

ELISA Kit for Activin A (ACVA)

產品介紹: 激活素 A (ACVA)檢測試劑盒 (酶聯免疫吸附試驗法) | 貨號AED245Hu (Indirect ELISA),偵測的靈敏度12.35-1,000pg/mL、最低靈敏度5.35pg/mL。ELISA原理為利用抗體與抗原之間的高度專一性,做為定量標誌的方法;無論測量抗原或測量抗體都有很高的靈敏度跟專一性。該檢測方法對激活素 A (ACVA)具有高靈敏度和出色的特異性。未觀察到激活素 A (ACVA)和類似物之間的顯著交叉反應或干擾。Cloud-Clone corp提供高靈敏度之ELISA (酵素免疫分析套組/酶聯免疫吸附試驗法),專門生產研究用檢測試劑的生技公司,於美國研發和生產,產品線包括ELISA Kit、CLIA Kit、Antibody及recombinant protein;ELISA產品系列超過6000種以上,物種齊全包括rat、mouse、rabbit、cavia、human、porcine、bovine、simian等多物種商品。品質保證。ELISA商品的型式多元, 提供三明治ELISA、競爭型ELISA、高靈敏度設計ELISA。Uncoated ELISA型式;包括Capture Antibody, Detection Antibody、Standard、Streptavidin-HRP、TMB Substrate, 96-well Plate。

激活素 A (ACVA)檢測試劑盒 (酶聯免疫吸附試驗法)套組內容

Reagents Quantity Reagents Quantity
Pre-coated, ready to use 96-well strip plate 1 Plate sealer for 96 wells 4
Standard 2 Standard Diluent 1×20mL
Detection Reagent A 1 Assay Diluent A 1×12mL
Detection Reagent B 1×120µL Assay Diluent B 1×12mL
Reagent Diluent 1×300µL Stop Solution 1×6mL
TMB Substrate 1×9mL Instruction manual 1
Wash Buffer (30 × concentrate) 1×20mL  

 

 激活素 A (ACVA)檢測試劑盒簡單流程

  1.  Prepare all reagents, samples and standards;
    2. Add 50µL standard or sample to each well.
    And then add 50µL prepared Detection Reagent A immediately.
    Shake and mix. Incubate 1 hour at 37°C;
    3. Aspirate and wash 3 times;
    4. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
    5. Aspirate and wash 5 times;
    6. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
    7. Add 50µL Stop Solution. Read at 450 nm immediately.

 激活素 A (ACVA)檢測試劑盒相關之抗體 | 蛋白質 | ELISA | CLIA產品

Catalog No. Related products for research use of Mus musculus (Mouse) Organism species Applications (RESEARCH USE ONLY!)
CEA001Mu ELISA Kit for Activin A (ACVA) Enzyme-linked immunosorbent assay for Antigen Detection.
LMA001Mu Magnetic Luminex Assay Kit for Activin A (ACVA) ,etc. Magnetic Luminex Assay for Antigen Detection.

 

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激活素 A (ACVA)  檢測試劑盒 (酶聯免疫吸附試驗法) | CLOUD CLONE貨號CEA001Hu

ELISA Kit for Activin A (ACVA)

CC CE_工作區域 1.jpg

產品介紹: 激活素 A (ACVA)檢測試劑盒 (酶聯免疫吸附試驗法) | 貨號AED245Hu (Indirect ELISA),偵測的靈敏度12.35-1,000pg/mL、最低靈敏度4.99pg/mL。ELISA原理為利用抗體與抗原之間的高度專一性,做為定量標誌的方法;無論測量抗原或測量抗體都有很高的靈敏度跟專一性。該檢測方法對激活素 A (ACVA)具有高靈敏度和出色的特異性。未觀察到激活素 A (ACVA)和類似物之間的顯著交叉反應或干擾。Cloud-Clone corp提供高靈敏度之ELISA (酵素免疫分析套組/酶聯免疫吸附試驗法),專門生產研究用檢測試劑的生技公司,於美國研發和生產,產品線包括ELISA Kit、CLIA Kit、Antibody及recombinant protein;ELISA產品系列超過6000種以上,物種齊全包括rat、mouse、rabbit、cavia、human、porcine、bovine、simian等多物種商品。品質保證。ELISA商品的型式多元, 提供三明治ELISA、競爭型ELISA、高靈敏度設計ELISA。Uncoated ELISA型式;包括Capture Antibody, Detection Antibody、Standard、Streptavidin-HRP、TMB Substrate, 96-well Plate。

激活素 A (ACVA)檢測試劑盒 (酶聯免疫吸附試驗法)套組內容

Reagents Quantity Reagents Quantity
Pre-coated, ready to use 96-well strip plate 1 Plate sealer for 96 wells 4
Standard 2 Standard Diluent 1×20mL
Detection Reagent A 1 Assay Diluent A 1×12mL
Detection Reagent B 1×120µL Assay Diluent B 1×12mL
Reagent Diluent 1×300µL Stop Solution 1×6mL
TMB Substrate 1×9mL Instruction manual 1
Wash Buffer (30 × concentrate) 1×20mL  

激活素 A (ACVA)檢測試劑盒簡單流程

  1. Prepare all reagents, samples and standards;
    2. Add 50µL standard or sample to each well.
    And then add 50µL prepared Detection Reagent A immediately.
    Shake and mix. Incubate 1 hour at 37°C;
    3. Aspirate and wash 3 times;
    4. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
    5. Aspirate and wash 5 times;
    6. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
    7. Add 50µL Stop Solution. Read at 450 nm immediately.

 激活素 A (ACVA)檢測試劑盒相關之抗體 | 蛋白質 | ELISA | CLIA產品

Catalog No. Related products for research use of Homo sapiens (Human) Organism species Applications (RESEARCH USE ONLY!)
RPA001Hu01 Recombinant Activin A (ACVA) Positive Control; Immunogen; SDS-PAGE; WB.
APA001Hu01 Active Activin A (ACVA) Cell culture; Activity Assays.
PAA001Hu01 Polyclonal Antibody to Activin A (ACVA) WB; ICC; IHC-P; IHC-F; ELISA.
SEA001Hu ELISA Kit for Activin A (ACVA) Enzyme-linked immunosorbent assay for Antigen Detection.
CEA001Hu ELISA Kit for Activin A (ACVA) Enzyme-linked immunosorbent assay for Antigen Detection.
SCA001Hu CLIA Kit for Activin A (ACVA) Chemiluminescent immunoassay for Antigen Detection.
LMA001Hu Magnetic Luminex Assay Kit for Activin A (ACVA) ,etc. Magnetic Luminex Assay for Antigen Detection.

 

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口蹄疫病毒抗原快速檢測試劑盒| Foot and Mouth Disease Virus Antigen Rapid Test Kit貨號abx092115

Foot and Mouth Disease Virus Antigen Rapid Test Kit

abbexa2-285x300.png

口蹄疫病毒抗原快速檢測試劑盒是一種基於膠體金的定性側流免疫層析法,用於檢測口蹄疫病毒抗原。Abbexa 的口蹄疫病毒抗原快速檢測試劑盒(Foot and Mouth Disease Virus Antigen Rapid Test Kit, abx092115) 基於金免疫層析法測定(gold immuno-chromatography assay , GICA)原理。樣品中存在的任何口蹄疫病毒抗原(Foot and Mouth Disease Virus Antigen)與膠體金顆粒標記(colloidal gold particle-labelled)的口蹄疫病毒抗原抗體(Foot and Mouth Disease Virus Antigen antibody)。當足部集中和樣本中的口腔疾病病毒抗原超過檢出限,出現顏色變化檢測線,結果為陽性。當口蹄疫病毒抗原濃度在樣品溶液小於檢出限,檢測線無顏色變化,結果為negative。

Target Foot and Mouth Disease Virus Antigen
Reactivity Pig, Cow, Goat, Sheep
Recommended dilutions Optimal dilutions/concentrations should be determined by the end user.
Storage Store at 2-30 °C.
Validity The validity for this kit is 12 months.
Availability Shipped within 5-12 working days.
Note

This product is for research use only.

Please note that our kits are optimised for detection of native samples, rather than recombinant proteins. We are unable to guarantee detection of recombinant proteins, as they may have different sequences or tertiary structures to the native protein.

 

抗原快篩檢測 (貨號) 抗原快篩檢測 (品項) 抗原快篩檢測 (操作手冊)
abx090729 瘧原蟲快速檢測試劑盒 | Plasmodium Rapid Test Kit 操作手冊
abx090709 瘧疾(惡性瘧原蟲抗原)快篩試劑 | Malaria (Plasmodium Falciparum Antigen) Rapid Test Kit 操作手冊
abx092092 人輪狀病毒 A 組 (Rota A) 快速檢測試劑盒 | Human Rotavirus group A (Rota A) Rapid Test Kit 操作手冊
abx092162 人類猴痘病毒抗原快篩試紙 | Human Monkeypox Virus Antigen (MPXV) Rapid Test Kit 操作手冊
abx092115 口蹄疫病毒抗原快速檢測試劑盒| Foot and Mouth Disease Virus Antigen Rapid Test Kit 操作手冊
abx092160 非洲豬瘟抗原快速檢測試劑盒| African Swine Fever Antigen Rapid Test Kit 操作手冊
abx090727 腺病毒抗原和輪狀病毒抗原快速檢測試劑盒 | Adenovirus Antigen and Rotavirus Antigen Rapid Test Kit 操作手冊

 

 血尿素氮(Blood Urea Nitrogen , BUN)檢測 | 肌酐檢測試劑盒(Creatinine Assay Kit)  | UA尿酸檢測試劑盒 | Calcium鈣檢測試劑盒 | 鎂檢測試劑盒

貨號 分析品項 產品資料下載
abx090684 血尿素氮(Blood Urea Nitrogen , BUN)檢測 操作手冊
abx298944 肌酐檢測試劑盒(Creatinine Assay Kit)  操作手冊
abx298824 UA尿酸檢測試劑盒 操作手冊
abx298871 Calcium鈣檢測試劑盒 操作手冊
abx298873 鎂檢測試劑盒 操作手冊
abx298918 亞硝酸鹽檢測試劑盒| Nitrite Assay Kit貨號abx298918 操作手冊
abx298986 總碳水化合物檢測試劑盒 操作手冊
abx096001 ALT丙氨酸轉氨酶檢測試劑盒| Alanine Transaminase assay kit 操作手冊
abx294012 髓過氧化物酶 (MPO) 檢測試劑盒| Myeloperoxidase (MPO) Assay Kit 操作手冊
 abx298894 硫醇檢測試劑盒 操作手冊
 abx298878 孔雀石綠磷酸鹽檢測試劑盒 操作手冊
 abx298825 銅藍蛋白檢測試劑盒| Ceruloplasmin Assay Kit 操作手冊
 abx097195 即用型IHC 檢測試劑盒| IHC Detection Kit for Mouse and Rabbit Primary Antibody 操作手冊
abx298849 脂肪酶檢測試劑盒| Lipase Assay Kit貨號abx298849 操作手冊
abx097981 硫代巴比妥酸反應物質 (TBARS) 檢測試劑盒| Thiobarbituric Acid Reactive Substances (TBARS) Assay Kit 操作手冊
abx298964 甲醛檢測試劑盒| Formaldehyde Assay Kit 操作手冊
abx298847 胃蛋白酶檢測試劑盒| Pepsin Assay Kit 操作手冊
abx298934  總酚測定試劑盒| Total Phenols Assay Kit 操作手冊
abx298947 糖化血清蛋白檢測試劑盒 | Glycated Serum Protein Assay Kit 操作手冊
abx298913 植酸酶檢測試劑盒 | Phytase Assay Kit 操作手冊
abx298889 糖原/肝糖檢測試劑盒 | Glycogen Assay Kit貨號abx298889 操作手冊
abx298916 果糖胺檢測試劑盒 | fructosamine Kit 操作手冊
abx298937 色氨酸 (Trp) 檢測試劑盒 | The Tryptophan (Trp) Assay Kit 操作手冊
abx298911 膽紅素檢測試劑盒 | Bilirubin Assay Kit 操作手冊
abx298941  類黃酮檢測試劑盒 | Flavonoid Assay Kit 操作手冊
abx298951 牛磺酸檢測試劑盒 | Taurine Assay Kit 操作手冊
abx298950 脂氧合酶檢測試劑盒 | The Lipoxygenase Assay Kit 操作手冊
abx298806 醛脫氫酶 (ALDH) 檢測試劑盒 | Aldehyde Dehydrogenase (ALDH) Assay Kit 操作手冊
abx298912 乙醇檢測試劑盒 | Ethanol Assay Kit 操作手冊
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高遷移率族蛋白 1 (HMGB1) ELISA 試劑盒 | Cloud clone貨號SEA399Hu

ELISA Kit for High Mobility Group Protein 1 (HMGB1)

 

cloud-clone-285x300.png

高遷移率族蛋白 1 (HMGB1) ELISA 試劑盒 (酶聯免疫吸附試驗法) | 貨號SEA399Hu,偵測的靈敏度62.5-4,000pg/mL、最低靈敏度28.3pg/mL。ELISA原理為利用抗體與抗原之間的高度專一性,做為定量標誌的方法;無論測量抗原或測量抗體都有很高的靈敏度跟專一性。Cloud-Clone corp提供高靈敏度之ELISA (酵素免疫分析套組/酶聯免疫吸附試驗法),專門生產研究用檢測試劑的生技公司,於美國研發和生產,產品線包括ELISA Kit、CLIA Kit、Antibody及recombinant protein;ELISA產品系列超過6000種以上,物種齊全包括rat、mouse、rabbit、cavia、human、porcine、bovine、simian等多物種商品。品質保證。ELISA商品的型式多元, 提供三明治ELISA、競爭型ELISA、高靈敏度設計ELISA。Uncoated ELISA型式;包括Capture Antibody, Detection Antibody、Standard、Streptavidin-HRP、TMB Substrate, 96-well Plate。

Specificity

This assay has high sensitivity and excellent specificity for detection of High Mobility Group Protein 1 (HMGB1).
No significant cross-reactivity or interference between High Mobility Group Protein 1 (HMGB1) and analogues was observed.

Recovery

Matrices listed below were spiked with certain level of recombinant High Mobility Group Protein 1 (HMGB1) and the recovery rates were calculated by comparing the measured value to the expected amount of High Mobility Group Protein 1 (HMGB1) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 96-105 101
EDTA plasma(n=5) 81-95 89
heparin plasma(n=5) 79-90 84

Reagents and materials provided

Reagents Quantity Reagents Quantity
Pre-coated, ready to use 96-well strip plate 1 Plate sealer for 96 wells 4
Standard 2 Standard Diluent 1×20mL
Detection Reagent A 1×120µL Assay Diluent A 1×12mL
Detection Reagent B 1×120µL Assay Diluent B 1×12mL
TMB Substrate 1×9mL Stop Solution 1×6mL
Wash Buffer (30 × concentrate) 1×20mL Instruction manual 1

Assay procedure summary

  1. Prepare all reagents, samples and standards;
    2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
    3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
    4. Aspirate and wash 3 times;
    5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
    6. Aspirate and wash 5 times;
    7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
    8. Add 50µL Stop Solution. Read at 450nm immediately.

高遷移率族蛋白 1 (HMGB1) 相關之抗體 | 蛋白質 | ELISA | CLIA產品

Catalog No. Related products for research use of Homo sapiens (Human) Organism species Applications (RESEARCH USE ONLY!)
APA399Hu02 Active High Mobility Group Protein 1 (HMGB1) Cell culture; Activity Assays.
EPA399Hu62 Eukaryotic High Mobility Group Protein 1 (HMGB1) Positive Control; Immunogen; SDS-PAGE; WB.
EPA399Hu63 Eukaryotic High Mobility Group Protein 1 (HMGB1) Positive Control; Immunogen; SDS-PAGE; WB.
EPA399Hu61 Eukaryotic High Mobility Group Protein 1 (HMGB1) Positive Control; Immunogen; SDS-PAGE; WB.
RPA399Hu02 Recombinant High Mobility Group Protein 1 (HMGB1) Positive Control; Immunogen; SDS-PAGE; WB.
APA399Hu61 Active High Mobility Group Protein 1 (HMGB1) Cell culture; Activity Assays.
RPA399Hu01 Recombinant High Mobility Group Protein 1 (HMGB1) Positive Control; Immunogen; SDS-PAGE; WB.
PAA399Hu06 Polyclonal Antibody to High Mobility Group Protein 1 (HMGB1) WB; IHC; ICC; IP.
PAA399Hu01 Polyclonal Antibody to High Mobility Group Protein 1 (HMGB1) WB; IHC; ICC; IP.
PAA399Hu02 Polyclonal Antibody to High Mobility Group Protein 1 (HMGB1) WB; IHC; ICC; IP.
LAA399Hu71 Biotin-Linked Polyclonal Antibody to High Mobility Group Protein 1 (HMGB1) WB; IHC; ICC.
LAA399Hu81 FITC-Linked Polyclonal Antibody to High Mobility Group Protein 1 (HMGB1) WB; IHC; ICC; IF.
MAA399Hu23 Monoclonal Antibody to High Mobility Group Protein 1 (HMGB1) WB; IHC; ICC; IP.
MAA399Hu24 Monoclonal Antibody to High Mobility Group Protein 1 (HMGB1) WB; IHC; ICC; IP.
MAA399Hu25 Monoclonal Antibody to High Mobility Group Protein 1 (HMGB1) WB; IHC; ICC; IP.
MAA399Hu27 Monoclonal Antibody to High Mobility Group Protein 1 (HMGB1) WB; IHC; ICC; IP.
MAA399Hu22 Monoclonal Antibody to High Mobility Group Protein 1 (HMGB1) WB; IHC; ICC; IP.
MAA399Hu21 Monoclonal Antibody to High Mobility Group Protein 1 (HMGB1) WB; IHC; ICC; IP.
SEA399Hu ELISA Kit for High Mobility Group Protein 1 (HMGB1) Enzyme-linked immunosorbent assay for Antigen Detection.
IEA399Hu Instant ELISA Kit for High Mobility Group Protein 1 (HMGB1) Enzyme-linked immunosorbent assay for Antigen Detection.
AEA399Hu ELISA Kit for Anti-High Mobility Group Protein 1 Antibody (Anti-HMGB1) Enzyme-linked immunosorbent assay for Antibody Detection.
SCA399Hu CLIA Kit for High Mobility Group Protein 1 (HMGB1) Chemiluminescent immunoassay for Antigen Detection.
LMA399Hu Magnetic Luminex Assay Kit for High Mobility Group Protein 1 (HMGB1) ,etc. Magnetic Luminex Assay for Antigen Detection.
KSA399Hu01 ELISA Kit DIY Materials for High Mobility Group Protein 1 (HMGB1) Main materials for "Do It(ELISA Kit) Yourself"
PGA399Hu01 Primer Pair for High Mobility Group Protein 1 (HMGB1) PCR

 

 

SEA133Mu ELISA Kit for Tumor Necrosis Factor Alpha (TNFa)
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SEA133Ra ELISA Kit for Tumor Necrosis Factor Alpha (TNFa)
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抗谷氨酸脫羧酶抗體 (Anti-GAD) 檢測試劑盒 (酶聯免疫吸附試驗法) | CLOUD CLONE貨號AED245Hu

ELISA Kit for Anti-Glutamic Acid Decarboxylase Antibody (Anti-GAD)

CC HE_工作區域 1.jpg

 

產品介紹: 抗谷氨酸脫羧酶抗體 (Anti-GAD)檢測試劑盒 (酶聯免疫吸附試驗法) | 貨號AED245Hu (Indirect ELISA),偵測的靈敏度1.56-100ng/mL、最低靈敏度0.59ng/mL。ELISA原理為利用抗體與抗原之間的高度專一性,做為定量標誌的方法;無論測量抗原或測量抗體都有很高的靈敏度跟專一性。該檢測方法對檢測抗谷氨酸脫羧酶抗體 (Anti-GAD)具有高靈敏度和出色的特異性。未觀察到抗谷氨酸脫羧酶抗體 (Anti-GAD)和類似物之間的顯著交叉反應或干擾。Cloud-Clone corp提供高靈敏度之ELISA (酵素免疫分析套組/酶聯免疫吸附試驗法),專門生產研究用檢測試劑的生技公司,於美國研發和生產,產品線包括ELISA Kit、CLIA Kit、Antibody及recombinant protein;ELISA產品系列超過6000種以上,物種齊全包括rat、mouse、rabbit、cavia、human、porcine、bovine、simian等多物種商品。品質保證。ELISA商品的型式多元, 提供三明治ELISA、競爭型ELISA、高靈敏度設計ELISA。Uncoated ELISA型式;包括Capture Antibody, Detection Antibody、Standard、Streptavidin-HRP、TMB Substrate, 96-well Plate。

 

抗谷氨酸脫羧酶抗體 (Anti-GAD)檢測試劑盒 (酶聯免疫吸附試驗法)套組內容

Reagents Quantity Reagents Quantity
Pre-coated, ready to use 96-well strip plate 1 Plate sealer for 96 wells 4
Standard 2 Standard Diluent 1×20mL
Detection Reagent A 1×120µL Assay Diluent A 1×12mL
TMB Substrate 1×9mL Stop Solution 1×6mL
Wash Buffer (30 × concentrate) 1×20mL Instruction manual 1

 抗谷氨酸脫羧酶抗體 (Anti-GAD)檢測試劑盒簡單流程

  1. Prepare all reagents, samples and standards;
    2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
    3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
    4. Aspirate and wash 5 times;
    5. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
    6. Add 50µL Stop Solution. Read at 450nm immediately..

 抗谷氨酸脫羧酶抗體 (Anti-GAD)檢測試劑盒相關之抗體 | 蛋白質 | ELISA | CLIA產品

Catalog No. Related products for research use of Homo sapiens (Human) Organism species Applications (RESEARCH USE ONLY!)
RPD245Hu01 Recombinant Glutamic Acid Decarboxylase (GAD) Positive Control; Immunogen; SDS-PAGE; WB.
AED245Hu ELISA Kit for Anti-Glutamic Acid Decarboxylase Antibody (Anti-GAD) Enzyme-linked immunosorbent assay for Antibody Detection.

 

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抗生長激素抗體 (Anti-GH) 檢測試劑盒 (酶聯免疫吸附試驗法) | CLOUD CLONE貨號AEA044Hu

ELISA Kit for Anti-Growth Hormone Antibody (Anti-GH)
抗生長激素抗體 (Anti-GH) 檢測試劑盒

產品介紹: 

CC HE_工作區域 1.jpg

抗生長激素抗體 (Anti-GH) 檢測試劑盒 (酶聯免疫吸附試驗法) | 貨號AEA044Hu (Indirect ELISA),偵測的靈敏度3.12-200ng/mL、最低靈敏度1.12ng/mL。ELISA原理為利用抗體與抗原之間的高度專一性,做為定量標誌的方法;無論測量抗原或測量抗體都有很高的靈敏度跟專一性。該檢測方法對檢測抗生長激素抗體 (Anti-GH)具有高靈敏度和出色的特異性。未觀察到抗生長激素抗體 (Anti-GH)和類似物之間的顯著交叉反應或干擾。Cloud-Clone corp提供高靈敏度之ELISA (酵素免疫分析套組/酶聯免疫吸附試驗法),專門生產研究用檢測試劑的生技公司,於美國研發和生產,產品線包括ELISA Kit、CLIA Kit、Antibody及recombinant protein;ELISA產品系列超過6000種以上,物種齊全包括rat、mouse、rabbit、cavia、human、porcine、bovine、simian等多物種商品。品質保證。ELISA商品的型式多元, 提供三明治ELISA、競爭型ELISA、高靈敏度設計ELISA。Uncoated ELISA型式;包括Capture Antibody, Detection Antibody、Standard、Streptavidin-HRP、TMB Substrate, 96-well Plate。

抗生長激素抗體 (Anti-GH) (酶聯免疫吸附試驗法)套組內容

Reagents Quantity Reagents Quantity
Pre-coated, ready to use 96-well strip plate 1 Plate sealer for 96 wells 4
Standard 2 Standard Diluent 1×20mL
Detection Reagent A 1×120µL Assay Diluent A 1×12mL
TMB Substrate 1×9mL Stop Solution 1×6mL
Wash Buffer (30 × concentrate) 1×20mL Instruction manual 1

 抗生長激素抗體 (Anti-GH)簡單流程

  1. Prepare all reagents, samples and standards;
    2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
    3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
    4. Aspirate and wash 5 times;
    5. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
    6. Add 50µL Stop Solution. Read at 450nm immediately. 

抗生長激素抗體 (Anti-GH)相關之抗體 | 蛋白質 | ELISA | CLIA產品

Catalog No. Related products for research use of Homo sapiens (Human) Organism species Applications (RESEARCH USE ONLY!)
APA044Hu01 Active Growth Hormone (GH) Cell culture; Activity Assays.
EPA044Hu61 Eukaryotic Growth Hormone (GH) Positive Control; Immunogen; SDS-PAGE; WB.
RPA044Hu01 Recombinant Growth Hormone (GH) Positive Control; Immunogen; SDS-PAGE; WB.
PAA044Hu06 Polyclonal Antibody to Growth Hormone (GH) WB; IHC; ICC; IP.
PAA044Hu01 Polyclonal Antibody to Growth Hormone (GH) WB; IHC; ICC; IP.
MAA044Hu22 Monoclonal Antibody to Growth Hormone (GH) WB; IHC; ICC; IP.
MAA044Hu26 Monoclonal Antibody to Growth Hormone (GH) WB; IHC; ICC; IP.
MAA044Hu21 Monoclonal Antibody to Growth Hormone (GH) WB; IHC; ICC; IP.
MAA044Hu23 Monoclonal Antibody to Growth Hormone (GH) WB; IHC; ICC; IP.
MAA044Hu24 Monoclonal Antibody to Growth Hormone (GH) WB; IHC; ICC; IP.
MAA044Hu25 Monoclonal Antibody to Growth Hormone (GH) WB; IHC; ICC; IP.
LAA044Hu72 Biotin-Linked Monoclonal Antibody to Growth Hormone (GH) WB; IHC; ICC.
SEA044Hu ELISA Kit for Growth Hormone (GH) Enzyme-linked immunosorbent assay for Antigen Detection.
CEA044Hu ELISA Kit for Growth Hormone (GH) Enzyme-linked immunosorbent assay for Antigen Detection.
AEA044Hu ELISA Kit for Anti-Growth Hormone Antibody (Anti-GH) Enzyme-linked immunosorbent assay for Antibody Detection.
SCA044Hu CLIA Kit for Growth Hormone (GH) Chemiluminescent immunoassay for Antigen Detection.
LMA044Hu Magnetic Luminex Assay Kit for Growth Hormone (GH) ,etc. Magnetic Luminex Assay for Antigen Detection.
PSA044Hu01 Antibody Pair for Growth Hormone (GH) ELISA; CLIA; ELISPOT; Luminex; Immunochromatography and other Immunoassays.
KSA044Hu01 ELISA Kit DIY Materials for Growth Hormone (GH) Main materials for "Do It (ELISA Kit) Yourself".
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胰島素 ELISA (INS)試劑盒 | Human Insulin (INS) ELISA Kit貨號KTE62671

Human Insulin (INS) ELISA Kit

產品描述:胰島素 ELISA (INS)試劑盒採用兩點夾心ELISA(two-site sandwich ELISA)定量樣品中的胰島素 (Insulin (INS)。對胰島素 (Insulin (INS)特異的抗體已預先包被在微孔板上。將標準品和樣品吸取到孔中,並且存在的任何胰島素 (Insulin (INS)都被固定的抗體結合。除去所有未結合的物質後,將HRP-綴合胰島素 (Insulin (INS)檢測抗體添加到孔中。洗滌以除去任何未結合的HRP試劑後,將色原溶液添加到孔中,並且根據在初始步驟中結合的胰島素 (Insulin (INS)的量成比例地顯色。停止顯色並測量顏色的強度。偵測範圍: 1 mU/L-16 mU/L, 最低靈敏度0.1 mU/L。可應用於以下樣品來源: Cell culture supernatants, Other biological fluids, Plasma, Serum

ABBKINE ELISA KIT 繪圖 (物種).jpg

Human Insulin (INS) ELISA Kit employs a two-site sandwich ELISA to quantitate Insulin in samples. An antibody specific for Insulin has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any Insulin present is bound by the immobilized antibody. After removing any unbound substances, HRP-Conjugate Human Insulin detection antibody is added to the wells. Following a wash to remove any unbound HRP reagent, a Chromogen solution is added to the wells and color develops in proportion to the amount of Insulin bound in the initial step. The color development is stopped and the intensity of the color is measured.

Product name Human Insulin (INS) ELISA Kit
Reactivity Human
Applications ELISA
Applications notes Human Insulin (INS) ELISA Kit employs a two-site sandwich ELISA to quantitate Insulin in samples. An antibody specific for Insulin has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any Insulin present is bound by the immobilized antibody. After removing any unbound substances, HRP-Conjugate Human Insulin detection antibody is added to the wells. Following a wash to remove any unbound HRP reagent, a Chromogen solution is added to the wells and color develops in proportion to the amount of Insulin bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA
Calibration range 1 mU/L-16 mU/L
Limit of detection 0.1 mU/L
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

使用注意事項

  • 請勿混用不同試劑盒中的組分,或在試劑盒到期日期之後使用試劑。
  • 打開之前,讓所有試劑至少加室溫30分鐘。
  • 用試劑預清洗移液器吸頭,為每個樣品,標準液和試劑使用新鮮的移液器吸頭,以免污染。
  • 未使用的孔必須在提供的密封袋中於4°C乾燥。
  • 徹底混合對於結果非常重要。建議每10分鐘使用低頻振盪器或稍稍搖動手。
  • 建議所有樣品和標準品一式兩份或一式三份進行測定。
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亞精胺 (SMD) ELISA 試劑盒| Spermidine (SMD) ELISA Kit貨號abx585001

偵測範圍0.78 ng/ml - 200 ng/ml

 

亞精胺 (SMD) ELISA 試劑盒是用於體外定量測定血清、血漿、組織勻漿、細胞裂解物、細胞培養上清液和其他生物體液中亞精胺 (SMD) 濃度的 ELISA 試劑盒。Spermidine (SMD) ELISA Kit is an ELISA Kit for the in vitro quantitative measurement of Spermidine (SMD) concentrations in serum, plasma, tissue homogenates, cell lysates, cell culture supernatants and other biological fluids.

abbexa2-285x300.png

產品介紹: 

亞精胺(Spermidine)是一種多胺化合物 (polyamine compound , C7H19N3),存在於核醣體(ribosomes)和活組織(living tissues)中,在生物體(organisms)內具有多種代謝功能(metabolic functions)。它最初是從精液(semen)中分離出來的。多胺(Polyamines),例如亞精胺(spermidine),是聚陽離子脂肪胺(polycationic aliphatic amines)並且是多功能(multifunctional)的。它們在細胞存活中起著至關重要的作用。亞精胺合酶 (Spermidine synthase ,SPDS) 催化腐胺(putrescine)形成亞精胺(spermidine)。亞精胺(spermidine)是進一步多胺(polyamines)的前體,例如精胺(spermine)及其結構異構體熱精胺(isomer thermospermine)。亞精胺(Spermidine synchronizes)同步一系列生物過程(例如 Ca2+、Na+、K+ -ATP 酶),從而維持膜電位並控制細胞內 pH 和體積。亞精胺調節生物過程,例如穀氨酸能 N 甲基-d-天冬氨酸受體(glutamatergic Nmethyl- d-aspartate receptor ,NMDA 受體)引起的 Ca2+ 流入,這與一氧化氮合酶 (nitric oxide synthase ,NOS) 和 cGMP/PKG 通路激活以及大腦皮層突觸體(cerebral cortex synaptosomes) Na+、K+-ATP 酶活性降低有關。

In

 

 

 

troduction

Spermidine is a polyamine compound (C7H19N3) found in ribosomes and living tissues, and having various metabolic functions within organisms. It was originally isolated from semen. Polyamines, such as spermidine, are polycationic aliphatic amines and are multifunctional. They serve vital roles in cell survival. Spermidine synthase (SPDS) catalyzes the formation of spermidine from putrescine. Spermidine is a precursor to further polyamines, such as spermine and its structural isomer thermospermine. Spermidine synchronizes an array of biological processes (such as Ca2+, Na+, K+ -ATPase) thus maintaining membrane potential and controlling intracellular pH and volume. Spermidine regulates biological processes, such as Ca2+ influx by glutamatergic Nmethyl- d-aspartate receptor (NMDA receptor), which has been associated with nitric oxide synthase (NOS) and cGMP/PKG pathway activation and a decrease of Na+,K+-ATPase activity in cerebral cortex synaptosomes.
Target Spermidine (SMD)
Reactivity General (All species)
Tested Applications ELISA
Recommended dilutions Optimal dilutions/concentrations should be determined by the end user.
Storage Shipped at 4 °C. Upon receipt, store the kit according to the storage instruction in the kit's manual.
Validity The validity for this kit is 6 months.
Stability The stability of the kit is determined by the rate of activity loss. The loss rate is less than 5% within the expiration date under appropriate storage conditions. To minimize performance fluctuations, operation procedures and lab conditions should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same user throughout.
Test Range 0.78 ng/ml - 200 ng/ml
Sensitivity < 0.29 ng/ml
Standard Form Lyophilized
Detection Method Colorimetric
Assay Type Competitive
Assay Data Quantitative
Sample Type Serum, plasma, tissue homogenates, cell lysates, cell culture supernatants and other biological fluids.
Target Type Antigen
Assay Principle This kit is based on competitive enzyme-linked immuno-sorbent assay technology. An antibody is pre-coated onto a 96-well plate. Standards, test samples, and biotin-conjugated reagent are added to the wells and incubated. A competitive inhibition reaction takes place between the biotin-labelled SMD and the unlabelled- SMD on the pre-coated antibody. The HRP-conjugated reagent is then added, and the whole plate is incubated. Unbound conjugates are removed using wash buffer at each stage. TMB substrate is used to quantify the HRP enzymatic reaction. After TMB substrate is added, only wells that contain sufficient SMD will produce a blue coloured product, which then changes to yellow after adding the acidic stop solution. The intensity of the color yellow is inversely proportional to the SMD amount bound on the plate. The OD is measured spectrophotometrically at 450 nm in a microplate reader, from which the concentration of SMD can be calculated.
Kit Components

The kit components listed are for reference only. The product manual may differ slightly. The product should be used as stated on the product manual included and delivered together with the product.

  • Pre-coated 96-Well Microplate
  • Standard
  • Standard Diluent Buffer
  • Wash Buffer
  • Detection Reagent A
  • Detection Reagent B
  • Diluent A
  • Diluent B
  • TMB Substrate
  • Stop Solution
  • Plate Sealer
Material Required But Not Provided
  • 37°C incubator
  • Multi and single channel pipettes and sterile pipette tips
  • Squirt bottle or automated microplate washer
  • 1.5 ml tubes
  • Distilled water
  • Absorbent filter papers
  • 100 ml and 1 liter graduated cylinders
  • Microplate reader (wavelength: 450 nm)
  • ELISA Shaker
Reagent Preparation

This procedure is provided for reference only. The product manual may differ slightly. The product should be used as stated on the product manual included and delivered together with the product.

  • 1) Standard: Prepare the standard with the recommended volume of Standard Diluent Buffer, to make the standard solution. Then use the Standard Diluent buffer to carry out serial dilutions of the standard solution, as instructed in the Protocol.
  • 2) Wash Buffer: Dilute the concentrated Wash Buffer with distilled water, as instructed in the Protocol.
  • 3) Detection Reagent Preparation: Calculate the total volume of working solution required. Dilute Detection Reagent A and Detection Reagent B with Diluent A and Diluent B, respectively, at 1:100.
Assay Procedure

This procedure is provided for reference only. The product manual may differ slightly. The product should be used as stated on the product manual included and delivered together with the product.

  • 1) Set standard, test samples and control wells.
  • 2) Aliquot 50 µl of diluted standard into the standard wells.
  • 3) Aliquot 50 µl of Standard Diluent buffer into the control (zero) well.
  • 4) Aliquot 50 µl of diluted samples into the sample wells.
  • 5) Immediately aliquot 50 µl of Detection Reagent A to each well. Incubate for 1 hr at 37 °C.
  • 6) Wash 3 times.
  • 7) Aliquot 100 µl of Detection Reagent B to each well. Incubate for 30 mins at 37 °C.
  • 8) Wash 5 times.
  • 9) Aliquot 90 µl of TMB Substrate to each well. Incubate for 10-20 mins at 37 °C.
  • 10) Aliquot 50 µl of Stop Solution.
  • 11) Measure the OD at 450 nm.
Protocol

This procedure is provided for reference only. The product manual may differ slightly. The product should be used as stated on the product manual included and delivered together with the product.

  • Equilibrate the kit components and samples to room temperature (18 - 25 °C) before use. It is recommended to plot a standard curve for each test.
  • 1. Set standard, test sample and control (zero) wells on the pre-coated plate respectively, and then, record their positions. It is recommended to measure each standard and sample at least in duplicate.
  • 2. Add 50 µL of each standard, control and sample into the appropriate wells.
  • 3. Remove the cover and discard the liquid.
  • 4. Immediately aliquot 50 µl of Detection Reagent A working solution. Seal the plate with a cover and incubate for 1 h at 37°C.
  • 5. Remove the cover and discard the solution. Wash the plate 3 times with 1X Wash Buffer.
  • 6. Add 100 µL of Detection Reagent B working solution into each well, seal and incubate at 37°C for 30 min.
  • 7. Discard the solution and wash the plate 5 times with wash buffer as explained in previous step.
  • 8. Aliquot 90 µl of TMB Substrate into each well. Seal the plate with a cover and incubate at 37°C for 10-20 min. Avoid exposure to light. The incubation time is for reference use only, the optimal time should be determined by end user. Do not exceed 30 min.
  • 9. Add 50 µL of Stop Solution to each well. Read at 450 nm immediately.
Results Calculation This assay is competitive, therefore there is an inverse correlation between SMD concentration in the sample and the absorbance measured. Create a graph with the log of the standard concentration (y-axis) and average absorbance measured (x-axis). Apply a best fit trendline through the standard points. The SMD concentration of the samples can be interpolated from the standard curve.
Assay Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, medium and high levels of Spermidine (SMD) were were tested 20 times on one plate, respectively.

Inter-assay Precision (Precision between assays): 3 samples with low, medium and high levels of Spermidine (SMD) were tested on 3 different plates, 8 replicates in each plate.

CV (%) = (Standard Deviation / mean) × 100

Intra-Assay: CV<10%

Inter-Assay: CV<12%

Availability Shipped within 1-2 weeks.
Note This product is for research use only.The range and sensitivity is subject to change. Please contact us for the latest product information. For accurate results, sample concentrations must be diluted to mid-range of the kit. If you require a specific range, please contact us in advance or write your request in your order comments.Please note that our ELISA and CLIA kits are optimised for detection of native samples, rather than recombinant proteins. We are unable to guarantee detection of recombinant proteins, as they may have different sequences or tertiary structures to the native protein.
Standard 200 ng/ml

 

Published in:
Pekar, T. et al. The positive effect of spermidine in older adults suffering from dementia. Wiener klinische Wochenschrift (2020).

Published in:
Pekar, T. et al. Spermidine in dementia. Wiener klinische Wochenschrift 132, 42-46 (2019).

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Human FGF basic/FGF2/bFGF試劑盒 | EliKine™ Human bFGF ELISA Kit貨號KET6006

15.6 pg/mL-1000 pg/mL, 最低靈敏度8 pg/mL

成纖維細胞生長因子2(fibroblast growth factor 2),也稱為鹼性成纖維細胞生長因子(bFGF)和FGF-β。FGF2 基因編碼的蛋白質是成纖維細胞生長因子 (fibroblast growth factor , FGF) 家族的成員。 FGF 家族成員(FGF family members)結合肝素(heparin)並具有廣泛的促有絲分裂(possess broad mitogenic)和血管生成活性(angiogenic activities)。這種蛋白質與多種生物過程有關,例如肢體(limb)和神經系統發育(nervous system development)、傷口癒合(wound healing)和腫瘤生長(tumor growth)。該基因的 mRNA 包含多個聚腺苷酸化位點(multiple polyadenylation sites),並且可以從非 AUG (CUG) 和 AUG 起始密碼子翻譯而來,從而產生具有不同特性的五種不同亞型。 CUG 啟動的亞型位於細胞核(nucleus)中並負責內分泌作用(intracrine effect),而 AUG 啟動的形式(AUG-initiated form)主要是細胞溶質(cytosolic)的並且負責該 FGF 的旁分泌(paracrine)和自分泌作用(autocrine effects)。

產品描述: bFGF ELISA試劑盒 (EliKine™ Human bFGF ELISA Kit 貨號KET6006) 採用兩點夾心ELISA (Sandwich ELISA)定量樣品中的bFGF。對bFGF特異的抗體已預先包被在微孔板上。將標準品和樣品吸取到孔中,並且存在的任何bFGF都被固定的抗體結合。除去所有未結合的物質後,將HRP-綴合bFGF檢測抗體添加到孔中。洗滌以除去任何未結合的HRP試劑後,將色原溶液添加到孔中,並且根據在初始步驟中結合的bFGF的量成比例地顯色。停止顯色並測量顏色的強度。偵測範圍: 15.6 pg/mL-1000 pg/mL, 最低靈敏度8 pg/mL可應用於以下樣品來源: Cell culture supernatants, Other biological fluids, Plasma, Serum。

 

ABBKINE ELISA KIT 繪圖 (物種).jpg

Product name EliKine™ Human bFGF ELISA Kit
Reactivity Human
Applications ELISA
Applications notes EliKine™ Human bFGF ELISA Kit employs a two-site sandwich ELISA to quantitate bFGF in samples. An antibody specific for bFGF has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any bFGF present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for bFGF is added to the wells. After washing, proprietary EliKine™ Streptavidin-HRP conjugates is added to the wells. Following a wash to remove any unbound streptavidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of bFGF bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative Basic fibroblast growth factor; BFGF; FGF 2; FGF B; FGF2; FGF2 basic; FGFB; Fibroblast growth factor 2 (basic); HBGF 2; HBGF2; HBGH 2; Heparin binding growth; factor 2; Heparin binding growth factor 2 precursor; OTTMUSP00000046776; Prostatropin
Kit components • Human bFGF microplate
• Human bFGF standard
• Human bFGF detect antibody
• Avidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers.
Features & Benefits EliKine™ Human bFGF ELISA Kit has high sensitivity and excellent specificity for detection of Human bFGF. No significant cross-reactivity or interference between Human bFGF and analogues was observed.
Calibration range 15.6 pg/mL-1000 pg/mL
Limit of detection 8 pg/mL

細胞因子(Cytokine)功能

細胞因子(Cytokine)具有多種生物學功能。它是急性期反應(acute phase response)的有效誘導劑。在B細胞(B-cells)最終分化為Ig分泌細胞(Ig-secreting cells)的最終分化中起重要作用,參與淋巴細胞(lymphocyte)和單核細胞(monocyte)的分化。它誘導骨髓瘤(myeloma)和漿細胞瘤(plasmacytoma growth)生長,並誘導神經細胞分化(differentiation)。作用於B細胞(B-cells),T細胞(T-cells),肝細胞(hepatocytes),造血祖細胞(hematopoeitic progenitor cells)和CNS中樞神經系統細胞。也可以作為肌肉因子(myokine)。它在肌肉收縮後排入血液,並起到增加脂肪分解和改善胰島素抵抗(insulin resistance)的作用。

炎性細胞因子

炎症由多種可溶性因子介導,包括一組稱為細胞因子的分泌多肽。炎性細胞因子可分為兩類:參與急性炎症的細胞因子和負責任的細胞因子。炎症是組織對損傷的反應,其特徵是在急性期以增加的血流量和血管通透性以及體液,白細胞和炎性介質(例如細胞因子)的積累為特徵。幾種細胞因子在介導急性炎症反應中起關鍵作用,即IL-1,TNF-α,IL-6,IL-11,IL-8和其他趨化因子,G-CSF和GM-CSF。

已知可介導慢性炎症過程的細胞因子可分為參與體液炎症的細胞因子,例如IL-3,IL-4,IL-5,IL-6,IL-7,IL-9,IL-10,IL- 13,轉化生長因子b(TGF-b),以及那些有助於細胞炎症的因子,例如IL-1,IL-2,IL-3,IL-4,IL-7,IL-9,IL-10, IL-12,干擾素(IFN),IFN-g誘導因子(IGIF),TGF-b和TNF-a和-b。炎症異常是一大類疾病,是許多人類疾病的基礎。細胞因子是宿主對感染,免疫反應,炎症和創傷反應的調節劑。一些細胞因子起使疾病惡化的作用(促炎細胞因子),而另一些細胞因子起減輕炎症和促進癒合的作用(抗炎細胞因子)。

白介素-1(Interleukin-1, IL-1)是有史以來最早的細胞因子(cytokines)之一。它的最初發現是可能誘發發燒(fever),控制淋巴細胞(lymphocytes),增加骨髓細胞數量(bone marrow cells)並引起骨關節退化的因素。此時,IL-1以其他幾種名稱為人所知,包括內源性熱原(endogenous pyrogen),淋巴細胞活化因子(lymphocyte activating factor),造血素-1(haemopoetin-1)和單核細胞因子(mononuclear cell factor)等。大約在1984年至1985年間,科學家們證實IL-1實際上由兩種不同的蛋白質組成,現在稱為IL-1α和IL-1β,它們屬於稱為白細胞介素1超家族的細胞因子家族。 IL-1β由巨噬細胞(macrophages),單核細胞(monocytes)和樹突狀細胞(dendritic cells)產生。

IFN-γ(IFN-gamma, 干擾素-γ)是促炎(proinflammatory)細胞因子(cytokine)的原型(prototype),由多種免疫細胞(immune cells)在炎性(inflammatory)條件下產生,特別是由T細胞(T cells)和NK細胞(NK cells)產生。它通過促進Th1細胞的發育(development)和激活(activation),單核細胞(monocytes)和巨噬細胞(macrophage)的化學引誘(chemoattraction)和激活(activation),抗原呈遞分子(antigen presentation molecules)的上調以及B細胞中的免疫球蛋白類別轉換(immunoglobulin class switching),在宿主防禦中起關鍵作用。它還具有抗病毒(antiviral),抗增殖(antiproliferative)和凋亡作用(apoptotic)。另外,IFN-γ通過促進調節性T細胞的發育並抑制Th17細胞分化而充當抗炎介質。 IFN-γ二聚體通過兩個IFN-γR1和兩個IFN-γR2亞基的受體複合物發出信號。

使用注意事項

  • 請勿混用不同試劑盒中的組分,或在試劑盒到期日期之後使用試劑。
  • 打開之前,讓所有試劑至少加室溫30分鐘。
  • 用試劑預清洗移液器吸頭,為每個樣品,標準液和試劑使用新鮮的移液器吸頭,以免污染。
  • 未使用的孔必須在提供的密封袋中於4°C乾燥。
  • 徹底混合對於結果非常重要。建議每10分鐘使用低頻振盪器或稍稍搖動手。
  • 建議所有樣品和標準品一式兩份或一式三份進行測定。
產品貨號  物種 品項內容 靈敏度 產品連結資料
KET7009 mouse Mouse IL-6 ELISA kit  7.5 pg/ml-120 pg/ml <<更多資料>>
KET6013 human 人白介素IL-1β(Interleukin 1β)試劑盒 7.8 pg/ml-500 pg/ml, <<更多資料>>
KET6017 human 人 IL-6 ELISA 試劑盒 3.125 pg/ml-200 pg/ml <<更多資料>>
KET6030 human TGF-β1(轉化生長因子β1)ELISA試劑盒 15.6 pg/ml-1000 pg/ml <<更多資料>>
KET6032 human TNF-α Alpha型腫瘤壞死因子 ELISA試劑盒 7.8 pg/ml-500 pg/ml <<更多資料>>
KET7005 mouse 白介素-1β(Interleukin-1β, IL-1β)ELISA試劑盒  15.6 pg/ml-1000 pg/ml <<更多資料>>
KET7014 Mouse TGF-beta1試劑盒 15.6 pg/ml-1000 pg/ml <<更多資料>>
KET7015 Mouse 小鼠 TNF-α ELISA 試劑盒 3.9 pg/mL-250 pg/mL <<更多資料>>
KET9001 human 白細胞介素 1 β檢測試劑盒 62.5 pg/ml-4000 pg/ml, <<更多資料>>
KET6011 Human  FN-γ試劑盒 7.8 pg/mL-500 pg/mL <<更多資料>>

 

產品貨號   品項內容 靈敏度 產品連結資料
KET7009 mouse Mouse IL-6 ELISA kit  7.5 pg/ml-120 pg/ml 更多資料
KET6013   human 人白介素IL-1β(Interleukin 1β)試劑盒 7.8 pg/ml-500 pg/ml, 更多資料
KET6017 human  IL-6 ELISA 試劑盒 3.125 pg/ml-200 pg/ml 更多資料
KET6030 human TGF-β1(轉化生長因子β1ELISA試劑盒 15.6 pg/ml-1000 pg/ml 更多資料
KET6032 human TNF-α Alpha型腫瘤壞死因子 ELISA試劑盒 7.8 pg/ml-500 pg/ml 更多資料
KET7005 mouse 白介素-1βInterleukin-1β, IL-1βELISA試劑盒  15.6 pg/ml-1000 pg/ml 更多資料
KET7014 Mouse TGF-beta1試劑盒 15.6 pg/ml-1000 pg/ml 更多資料
KET7015 Mouse 小鼠 TNF-α ELISA 試劑盒 3.9 pg/mL-250 pg/mL 更多資料
KET9001 human 白細胞介素 1 β檢測試劑盒 62.5 pg/ml-4000 pg/ml, 更多資料
KET6011 Human  FN-γ試劑盒 7.8 pg/mL-500 pg/mL 更多資料

 

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Human FN-γ試劑盒 | EliKine™ Human FN-γELISA Kit貨號KET6011 (7.8 pg/mL-500 pg/mL, 最低靈敏度4 pg/mL)

產品描述:IFN-γELISA試劑盒採用兩點夾心ELISA (Sandwich ELISA)定量樣品中的IFN-γ。對IFN-γ特異的抗體已預先包被在微孔板上。將標準品和樣品吸取到孔中,並且存在的任何IFN-γ都被固定的抗體結合。除去所有未結合的物質後,將HRP-綴合IFN-γ檢測抗體添加到孔中。洗滌以除去任何未結合的HRP試劑後,將色原溶液添加到孔中,並且根據在初始步驟中結合的IFN-γ的量成比例地顯色。停止顯色並測量顏色的強度。偵測範圍: 7.8 pg/mL-500 pg/mL, 最低靈敏度4 pg/mL可應用於以下樣品來源: Cell culture supernatants, Other biological fluids, Plasma, Serum

ABBKINE ELISA KIT 繪圖 (物種).jpg

Product name

EliKine™ Human IFN-γ ELISA Kit

Reactivity

Human

Applications

ELISA

Applications notes

EliKine™ Human IFN-γ ELISA Kit employs a two-site sandwich ELISA to quantitate IFN-γ in samples. An antibody specific for IFN-γ has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any IFN-γ present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for IFN-γ is added to the wells. After washing, proprietary EliKine™ Streptavidin-HRP conjugates is added to the wells. Following a wash to remove any unbound streptavidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of IFN-γ bound in the initial step. The color development is stopped and the intensity of the color is measured.

Detection method

Colorimetric

SampleType

Cell culture supernatants, Other biological fluids, Plasma, Serum

Assay type

Sandwich ELISA (quantitative)

Assay duration

Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.

Alternative

IFG; IFI; IFN gamma; IFN Immune; IFNG; Interferon gamma; Interferon Gamma Precursor; Macrophage Activating Factor; MAF; T Cell Interferon; Type II Interferon

Kit components

• Human IFN-γ microplate
• Human IFN-γ standard
• Human IFN-γ detect antibody
• EliKine™ Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers.

Features & Benefits

EliKine™ Human IFN-γ ELISA Kit has high sensitivity and excellent specificity for detection of Human IFN-γ. No significant cross-reactivity or interference between Human IFN-γ and analogues was observed.

Calibration range

7.8 pg/mL-500 pg/mL

Limit of detection

4 pg/mL

ABBKINE型錄第一頁_page-0001.jpg

細胞因子(Cytokine)功能

細胞因子(Cytokine)具有多種生物學功能。它是急性期反應(acute phase response)的有效誘導劑。在B細胞(B-cells)最終分化為Ig分泌細胞(Ig-secreting cells)的最終分化中起重要作用,參與淋巴細胞(lymphocyte)和單核細胞(monocyte)的分化。它誘導骨髓瘤(myeloma)和漿細胞瘤(plasmacytoma growth)生長,並誘導神經細胞分化(differentiation)。作用於B細胞(B-cells)T細胞(T-cells),肝細胞(hepatocytes),造血祖細胞(hematopoeitic progenitor cells)CNS中樞神經系統細胞。也可以作為肌肉因子(myokine)。它在肌肉收縮後排入血液,並起到增加脂肪分解和改善胰島素抵抗(insulin resistance)的作用。

炎性細胞因子

炎症由多種可溶性因子介導,包括一組稱為細胞因子的分泌多肽。炎性細胞因子可分為兩類:參與急性炎症的細胞因子和負責任的細胞因子。炎症是組織對損傷的反應,其特徵是在急性期以增加的血流量和血管通透性以及體液,白細胞和炎性介質(例如細胞因子)的積累為特徵。幾種細胞因子在介導急性炎症反應中起關鍵作用,即IL-1TNF-αIL-6IL-11IL-8和其他趨化因子,G-CSFGM-CSF

已知可介導慢性炎症過程的細胞因子可分為參與體液炎症的細胞因子,例如IL-3IL-4IL-5IL-6IL-7IL-9IL-10IL- 13,轉化生長因子bTGF-b),以及那些有助於細胞炎症的因子,例如IL-1IL-2IL-3IL-4IL-7IL-9IL-10 IL-12,干擾素(IFN),IFN-g誘導因子(IGIF),TGF-bTNF-a-b。炎症異常是一大類疾病,是許多人類疾病的基礎。細胞因子是宿主對感染,免疫反應,炎症和創傷反應的調節劑。一些細胞因子起使疾病惡化的作用(促炎細胞因子),而另一些細胞因子起減輕炎症和促進癒合的作用(抗炎細胞因子)。

白介素-1Interleukin-1, IL-1)是有史以來最早的細胞因子(cytokines)之一。它的最初發現是可能誘發發燒(fever),控制淋巴細胞(lymphocytes),增加骨髓細胞數量(bone marrow cells)並引起骨關節退化的因素。此時,IL-1以其他幾種名稱為人所知,包括內源性熱原(endogenous pyrogen),淋巴細胞活化因子(lymphocyte activating factor),造血素-1(haemopoetin-1)和單核細胞因子(mononuclear cell factor)等。大約在1984年至1985年間,科學家們證實IL-1實際上由兩種不同的蛋白質組成,現在稱為IL-1αIL-1β,它們屬於稱為白細胞介素1超家族的細胞因子家族。 IL-1β由巨噬細胞(macrophages),單核細胞(monocytes)和樹突狀細胞(dendritic cells)產生。

IFN-γIFN-gamma, 干擾素)是促炎(proinflammatory)細胞因子(cytokine)的原型(prototype),由多種免疫細胞(immune cells)在炎性(inflammatory)條件下產生,特別是由T細胞(T cells)NK細胞(NK cells)產生。它通過促進Th1細胞的發育(development)和激活(activation),單核細胞(monocytes)和巨噬細胞(macrophage)的化學引誘(chemoattraction)和激活(activation),抗原呈遞分子(antigen presentation molecules)的上調以及B細胞中的免疫球蛋白類別轉換(immunoglobulin class switching),在宿主防禦中起關鍵作用。它還具有抗病毒(antiviral),抗增殖(antiproliferative)和凋亡作用(apoptotic)。另外,IFN-γ通過促進調節性T細胞的發育並抑制Th17細胞分化而充當抗炎介質。 IFN-γ二聚體通過兩個IFN-γR1和兩個IFN-γR2亞基的受體複合物發出信號。

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產品貨號  物種 品項內容 靈敏度 產品連結資料
KET7009 mouse Mouse IL-6 ELISA kit  7.5 pg/ml-120 pg/ml <<更多資料>>
KET6013   human 人白介素IL-1β(Interleukin 1β)試劑盒 7.8 pg/ml-500 pg/ml, <<更多資料>>
KET6017 human 人 IL-6 ELISA 試劑盒 3.125 pg/ml-200 pg/ml <<更多資料>>
KET6030 human TGF-β1(轉化生長因子β1ELISA試劑盒 15.6 pg/ml-1000 pg/ml <<更多資料>>
KET6032 human TNF-α Alpha型腫瘤壞死因子 ELISA試劑盒 7.8 pg/ml-500 pg/ml <<更多資料>>
KET7005 mouse 白介素-1β(Interleukin-1β, IL-1β)ELISA試劑盒  15.6 pg/ml-1000 pg/ml <<更多資料>>
KET7014 Mouse TGF-beta1試劑盒 15.6 pg/ml-1000 pg/ml <<更多資料>>
KET7015 Mouse 小鼠 TNF-α ELISA 試劑盒 3.9 pg/mL-250 pg/mL <<更多資料>>
KET9001 human 白細胞介素 1 β檢測試劑盒 62.5 pg/ml-4000 pg/ml, <<更多資料>>
KET6011 Human  FN-γ試劑盒 7.8 pg/mL-500 pg/mL <<更多資料>>

 

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Coenzyme Ⅰ NAD(H) 分析試劑盒| Micro Coenzyme Ⅰ NAD(H) Assay Kit貨號KTB1020

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煙醯胺腺苷二核苷酸(Nicotinamide adenine dinucleotide , NAD)是一種酶共體(enzymatic cofactor),涉及許多氧化還原反應(redox reactions)。NAD作為電子載體(redox reactions),在氧化 (NAD) 和還原 (NADH) 形式之間迴圈。NAD 除了在紅氧反應中的作用外,在 ADP-核糖酸反應中扮演重要的作用,並且作為sirtuins的substrate。Nicotinamide adenine dinucleotide (NAD) is an enzymatic cofactor involved in many redox reactions. NAD functions as an electron carrier, cycling between the oxidized (NAD) and reduced (NADH) forms. In addition to its role in redox reactions, NAD plays critical roles in ADP-ribosylation reactions and as a substrate for sirtuins.

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Fig. Standard Curve of NAD in 96-well plate assay. The y-axis is ΔOD and the x-axis is NAD concentration (uM).
Abbkine Micro Coenzyme Ⅰ NAD(H) Assay Kit(貨號KTB1020), 檢測各種組織和細胞各種胞器中NAD 和 NADH比例。此試劑盒基於酶循環反應 (它不辨認 NADP+/NADPH),其中形成的 NADH 減少了 formazan (MTT) 。以波長 OD565 nm 下測量,吸收光的強度與樣品中的 NAD+ /NADH 濃度成正比。 96 孔板測定中的檢測限值為 0.78 μM 線性最高 50 μM NAD+/NADH。

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it components • Assay Buffer
• EtOH Solution
• WST-8 Solution
• Enhancer
• NAD Cycling Enzyme Mix
• NAD standard (10 mM)
• NAD Extraction Buffer
• NADH Extraction Buffer
Features & Benefits • Detect NAD+/NADH concentrations and ratios in cell or tissue extracts.
• Detection limit of 0.78 µM and linearity up to 50 µM NAD+/NADH in 96-well plate assay.
Calibration range 0.78 µM-50 µM
Usage notes • Addition of Working Reagent should be quick and mixing should be brief but thorough.
• The following substances interfere and should be avoided in sample preparation. EDTA (>0.5 mM), ascorbic acid, SDS (>0.2%), sodium azide, NP-40 (>1%) and Tween-20 (>1%).
Storage instructions Storage at –20°C and Keep from light immediately upon receipt. Kit has a storage time of 6 months from receipt. Refer to list of materials supplied for storage conditions of individual components.
Shipping Gel pack with blue ice.

活細胞和死細胞雙染色套組  |  CCK-8細胞增殖和細胞毒性試劑盒  | LDH細胞毒性測定試劑盒 | 細胞衰老檢測試劑盒(β-半乳糖苷酶 | 細胞增殖EdU Image試劑盒 

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NAMPT maintains mitochondria content via NRF2-PPARα/AMPKα pathway to promote cell survival under oxidative stress

A Yu, R Zhou, B Xia. Cellular Signalling

 

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TGF-β1(轉化生長因子β1)ELISA試劑盒 | EliKine™ Human TGF-β1 ELISA Kit 貨號KET6030 (15.6 pg/ml-1000 pg/ml, 最低靈敏度8 pg/mL)

轉化生長因子β1或TGF-β1是細胞激素轉化生長因子β超家族的多肽成員。它是一種分泌性蛋白質,執行許多細胞功能,包括控制細胞生長 ,細胞增殖,細胞分化和凋亡。TGF-β 能夠產生多種效應,幾乎所有細胞類型都以某種方式對此因子作出反應。活性 TGF-β1 的不當存在與多種病理狀況(pathological conditions)有關。由於必須嚴格調節其活性,TGF-β 以非活性複合物(inactive complex)的形式由細胞分泌。該複合物由 TGF-β1 與一種稱為潛伏相關肽 (latency associated peptide , LAP) 的蛋白質非共價結合而成。 TGF-β1 和 LAP 代表前肽(LAP represent of pro-peptide)的成分,前肽在分泌(secretion)前在高爾基體(golgi compartment)後隔室中被切割。 LAP 和 TGF-β1 均由二硫鍵(disulfide-linked)連接的同型二聚體(homodimer)組成,這兩種成分的結合使 TGF-β1 失活並且無法被抗 TGF-β 抗體接近。

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產品描述:TGF-β1(轉化生長因子β1)ELISA試劑盒採用兩點夾心ELISA(Sandwich ELISA)定量樣品中的TGF-β1(轉化生長因子β1)。對TGF-β1(轉化生長因子β1)特異的抗體已預先包被在微孔板上。將標準品和樣品吸取到孔中,並且存在的任何TGF-β1(轉化生長因子β1)都被固定的抗體結合。除去所有未結合的物質後,將HRP-綴合TGF-β1(轉化生長因子β1)檢測抗體添加到孔中。洗滌以除去任何未結合的HRP試劑後,將色原溶液添加到孔中,並且根據在初始步驟中結合的TGF-β1(轉化生長因子β1)的量成比例地顯色。停止顯色並測量顏色的強度。偵測範圍: 15.6 pg/ml-1000 pg/ml, 最低靈敏度8 pg/mL可應用於以下樣品來源: Cell culture supernatants, Other biological fluids, Plasma, Serum

 

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Product name EliKine™ Human TGF-β1 ELISA Kit
Reactivity Human, Mouse, Rat
Applications ELISA
Applications notes EliKine™ Human TGF-β1 ELISA Kit employs a two-site sandwich ELISA to quantitate TGF-β1 in samples. An antibody specific for TGF-β1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any TGF-β1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TGF-β1 is added to the wells. After washing, proprietary EliKine™ Streptavidin-HRP conjugates is added to the wells. Following a wash to remove any unbound streptavidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TGF-β1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative TGFB1; CED; DPD1; LAP; TGFB; TGFbeta; TGF-beta 1 protein; latency-associated peptide
Kit components • Human TGF-β1 microplate
• Human TGF-β1 standard
• Human TGF-β1 detect antibody
• EliKine™ Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers.
Features & Benefits EliKine™ Human TGF-β1 ELISA Kit has high sensitivity and excellent specificity for detection of Human TGF-β1. No significant cross-reactivity or interference between Human TGF-β1 and analogues was observed.
Calibration range 15.6 pg/ml-1000 pg/ml
Limit of detection 8 pg/mL
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background TGF-β is capable of producing a variety of effects and virtually all cell types respond to this factor in some way. The inappropriate presence of active TGF-β1 has been implicated in a variety of pathological conditions Because of the necessity for regulating its activity tightly, TGF-β is secreted by cells in the form of an inactive complex. This complex consists of TGF-β1 associated non-covalently with a protein designated the latency associated peptide (LAP). TGF-β1 and LAP represent components of a pro-peptide that is cleaved in a post-golgi compartment prior to secretion. LAP and TGF-β1 each consist of a disulfide-linked homodimer and the association of these two components renders TGF-β1 inactive and inaccessible to anti-TGF-β antibodies.
Gene ID 7040
Alternative TGFB1; CED; DPD1; LAP; TGFB; TGFbeta; TGF-beta 1 protein; latency-associated peptide
Accession P01137

細胞因子(Cytokine)功能

細胞因子(Cytokine)具有多種生物學功能。它是急性期反應(acute phase response)的有效誘導劑。在B細胞(B-cells)最終分化為Ig分泌細胞(Ig-secreting cells)的最終分化中起重要作用,參與淋巴細胞(lymphocyte)和單核細胞(monocyte)的分化。它誘導骨髓瘤(myeloma)和漿細胞瘤(plasmacytoma growth)生長,並誘導神經細胞分化(differentiation)。作用於B細胞(B-cells),T細胞(T-cells),肝細胞(hepatocytes),造血祖細胞(hematopoeitic progenitor cells)和CNS中樞神經系統細胞。也可以作為肌肉因子(myokine)。它在肌肉收縮後排入血液,並起到增加脂肪分解和改善胰島素抵抗(insulin resistance)的作用。

炎性細胞因子

炎症由多種可溶性因子介導,包括一組稱為細胞因子的分泌多肽。炎性細胞因子可分為兩類:參與急性炎症的細胞因子和負責任的細胞因子。炎症是組織對損傷的反應,其特徵是在急性期以增加的血流量和血管通透性以及體液,白細胞和炎性介質(例如細胞因子)的積累為特徵。幾種細胞因子在介導急性炎症反應中起關鍵作用,即IL-1,TNF-α,IL-6,IL-11,IL-8和其他趨化因子,G-CSF和GM-CSF。

已知可介導慢性炎症過程的細胞因子可分為參與體液炎症的細胞因子,例如IL-3,IL-4,IL-5,IL-6,IL-7,IL-9,IL-10,IL- 13,轉化生長因子b(TGF-b),以及那些有助於細胞炎症的因子,例如IL-1,IL-2,IL-3,IL-4,IL-7,IL-9,IL-10, IL-12,干擾素(IFN),IFN-g誘導因子(IGIF),TGF-b和TNF-a和-b。炎症異常是一大類疾病,是許多人類疾病的基礎。細胞因子是宿主對感染,免疫反應,炎症和創傷反應的調節劑。一些細胞因子起使疾病惡化的作用(促炎細胞因子),而另一些細胞因子起減輕炎症和促進癒合的作用(抗炎細胞因子)。

白介素-1(Interleukin-1, IL-1)是有史以來最早的細胞因子(cytokines)之一。它的最初發現是可能誘發發燒(fever),控制淋巴細胞(lymphocytes),增加骨髓細胞數量(bone marrow cells)並引起骨關節退化的因素。此時,IL-1以其他幾種名稱為人所知,包括內源性熱原(endogenous pyrogen),淋巴細胞活化因子(lymphocyte activating factor),造血素-1(haemopoetin-1)和單核細胞因子(mononuclear cell factor)等。大約在1984年至1985年間,科學家們證實IL-1實際上由兩種不同的蛋白質組成,現在稱為IL-1α和IL-1β,它們屬於稱為白細胞介素1超家族的細胞因子家族。 IL-1β由巨噬細胞(macrophages),單核細胞(monocytes)和樹突狀細胞(dendritic cells)產生。

IFN-γ(IFN-gamma, 干擾素-γ)是促炎(proinflammatory)細胞因子(cytokine)的原型(prototype),由多種免疫細胞(immune cells)在炎性(inflammatory)條件下產生,特別是由T細胞(T cells)和NK細胞(NK cells)產生。它通過促進Th1細胞的發育(development)和激活(activation),單核細胞(monocytes)和巨噬細胞(macrophage)的化學引誘(chemoattraction)和激活(activation),抗原呈遞分子(antigen presentation molecules)的上調以及B細胞中的免疫球蛋白類別轉換(immunoglobulin class switching),在宿主防禦中起關鍵作用。它還具有抗病毒(antiviral),抗增殖(antiproliferative)和凋亡作用(apoptotic)。另外,IFN-γ通過促進調節性T細胞的發育並抑制Th17細胞分化而充當抗炎介質。 IFN-γ二聚體通過兩個IFN-γR1和兩個IFN-γR2亞基的受體複合物發出信號。

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EliKine™ 人 IL-6 ELISA 試劑盒 | EliKine™ Human IL-6 ELISA Kit貨號KET6017 (3.125 pg/ml-200 pg/ml, 最低靈敏度2 pg/mL)

產品描述:IL-6(interleukin 6)ELISA試劑盒採用兩點夾心ELISA (Sandwich ELISA)定量樣品中的IL-6(interleukin 6)。對IL-6(interleukin 6)特異的抗體已預先包被在微孔板上。將標準品和樣品吸取到孔中,並且存在的任何IL-6(interleukin 6)都被固定的抗體結合。除去所有未結合的物質後,將HRP-綴合IL-6(interleukin 6)檢測抗體添加到孔中。洗滌以除去任何未結合的HRP試劑後,將色原溶液添加到孔中,並且根據在初始步驟中結合的IL-6(interleukin 6)的量成比例地顯色。停止顯色並測量顏色的強度。偵測範圍: 3.125 pg/ml-200 pg/ml, 最低靈敏度2 pg/mL可應用於以下樣品來源: Cell culture supernatants, Other biological fluids, Plasma, Serum。

 

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Product name EliKine™ Human IL-6 ELISA Kit
Reactivity Human
Applications ELISA
Applications notes EliKine™ Human IL-6 ELISA Kit employs a two-site sandwich ELISA to quantitate IL-6 in samples. An antibody specific for IL-6 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any IL-6 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for IL-6 is added to the wells. After washing, proprietary EliKine™ Streptavidin-HRP conjugates is added to the wells. Following a wash to remove any unbound streptavidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of IL-6 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative IL 6; IL6; BSF2; HGF; HSF; IFNB2; IL-6; B cell stimulatory factor-2; B-cell differentiation factor; CTL differentiation factor; OTTHUMP00000158544; hybridoma growth factor; interleukin 6; interleukin BSF-2
Kit components • Human IL-6 microplate
• Human IL-6 standard
• Human IL-6 detect antibody
• EliKine™ Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers.
Features & Benefits EliKine™ Human IL-6 ELISA Kit has high sensitivity and excellent specificity for detection of Human IL-6. No significant cross-reactivity or interference between Human IL-6 and analogues was observed.
Calibration range 3.125 pg/ml-200 pg/ml
Limit of detection 2 pg/mL

細胞因子(Cytokine)功能

細胞因子(Cytokine)具有多種生物學功能。它是急性期反應(acute phase response)的有效誘導劑。在B細胞(B-cells)最終分化為Ig分泌細胞(Ig-secreting cells)的最終分化中起重要作用,參與淋巴細胞(lymphocyte)和單核細胞(monocyte)的分化。它誘導骨髓瘤(myeloma)和漿細胞瘤(plasmacytoma growth)生長,並誘導神經細胞分化(differentiation)。作用於B細胞(B-cells),T細胞(T-cells),肝細胞(hepatocytes),造血祖細胞(hematopoeitic progenitor cells)和CNS中樞神經系統細胞。也可以作為肌肉因子(myokine)。它在肌肉收縮後排入血液,並起到增加脂肪分解和改善胰島素抵抗(insulin resistance)的作用。

炎性細胞因子

炎症由多種可溶性因子介導,包括一組稱為細胞因子的分泌多肽。炎性細胞因子可分為兩類:參與急性炎症的細胞因子和負責任的細胞因子。炎症是組織對損傷的反應,其特徵是在急性期以增加的血流量和血管通透性以及體液,白細胞和炎性介質(例如細胞因子)的積累為特徵。幾種細胞因子在介導急性炎症反應中起關鍵作用,即IL-1,TNF-α,IL-6,IL-11,IL-8和其他趨化因子,G-CSF和GM-CSF。

已知可介導慢性炎症過程的細胞因子可分為參與體液炎症的細胞因子,例如IL-3,IL-4,IL-5,IL-6,IL-7,IL-9,IL-10,IL- 13,轉化生長因子b(TGF-b),以及那些有助於細胞炎症的因子,例如IL-1,IL-2,IL-3,IL-4,IL-7,IL-9,IL-10, IL-12,干擾素(IFN),IFN-g誘導因子(IGIF),TGF-b和TNF-a和-b。炎症異常是一大類疾病,是許多人類疾病的基礎。細胞因子是宿主對感染,免疫反應,炎症和創傷反應的調節劑。一些細胞因子起使疾病惡化的作用(促炎細胞因子),而另一些細胞因子起減輕炎症和促進癒合的作用(抗炎細胞因子)。

白介素-1(Interleukin-1, IL-1)是有史以來最早的細胞因子(cytokines)之一。它的最初發現是可能誘發發燒(fever),控制淋巴細胞(lymphocytes),增加骨髓細胞數量(bone marrow cells)並引起骨關節退化的因素。此時,IL-1以其他幾種名稱為人所知,包括內源性熱原(endogenous pyrogen),淋巴細胞活化因子(lymphocyte activating factor),造血素-1(haemopoetin-1)和單核細胞因子(mononuclear cell factor)等。大約在1984年至1985年間,科學家們證實IL-1實際上由兩種不同的蛋白質組成,現在稱為IL-1α和IL-1β,它們屬於稱為白細胞介素1超家族的細胞因子家族。 IL-1β由巨噬細胞(macrophages),單核細胞(monocytes)和樹突狀細胞(dendritic cells)產生。

IFN-γ(IFN-gamma, 干擾素-γ)是促炎(proinflammatory)細胞因子(cytokine)的原型(prototype),由多種免疫細胞(immune cells)在炎性(inflammatory)條件下產生,特別是由T細胞(T cells)和NK細胞(NK cells)產生。它通過促進Th1細胞的發育(development)和激活(activation),單核細胞(monocytes)和巨噬細胞(macrophage)的化學引誘(chemoattraction)和激活(activation),抗原呈遞分子(antigen presentation molecules)的上調以及B細胞中的免疫球蛋白類別轉換(immunoglobulin class switching),在宿主防禦中起關鍵作用。它還具有抗病毒(antiviral),抗增殖(antiproliferative)和凋亡作用(apoptotic)。另外,IFN-γ通過促進調節性T細胞的發育並抑制Th17細胞分化而充當抗炎介質。 IFN-γ二聚體通過兩個IFN-γR1和兩個IFN-γR2亞基的受體複合物發出信號。

LINE群發(RT-9600).jpg

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白介素-6(Interleukin-6, IL-6)ELISA試劑盒 | EliKine™ Mouse IL-6 ELISA Kit貨號KET7009  7.5 pg/ml-120 pg/ml, 最低靈敏度0.5 pg/ml

產品描述:白介素-6(Interleukin-6, IL-6)ELISA試劑盒採用兩點夾心ELISA(Sandwich ELISA)定量樣品中的白介素-6(Interleukin-6, IL-6)。對白介素-6(Interleukin-6, IL-6)特異的抗體已預先包被在微孔板上。將標準品和样品吸取到孔中,並且存在的任何白介素-6(Interleukin-6, IL-6)都被固定的抗體結合。除去所有未結合的物質後,將HRP-綴合白介素-6(Interleukin-6, IL-6)檢測抗體添加到孔中。洗滌以除去任何未結合的HRP試劑後,將色原溶液添加到孔中,並且根據在初始步驟中結合的白介素-6(Interleukin-6, IL-6)的量成比例地顯色。停止顯色並測量顏色的強度。偵測範圍: 7.5 pg/ml-120 pg/ml, 最低靈敏度0.5 pg/ml可應用於以下樣品來源: Cell culture supernatants, Other biological fluids, Plasma, Serum

 

ABBKINE型錄第一頁_page-0001.jpg

ABBKINE ELISA KIT 繪圖 (物種).jpg

細胞因子(Cytokine)功能

細胞因子(Cytokine)具有多種生物學功能。它是急性期反應(acute phase response)的有效誘導劑。在B細胞(B-cells)最終分化為Ig分泌細胞(Ig-secreting cells)的最終分化中起重要作用,參與淋巴細胞(lymphocyte)和單核細胞(monocyte)的分化。它誘導骨髓瘤(myeloma)和漿細胞瘤(plasmacytoma growth)生長,並誘導神經細胞分化(differentiation)。作用於B細胞(B-cells),T細胞(T-cells),肝細胞(hepatocytes),造血祖細胞(hematopoeitic progenitor cells)和CNS中樞神經系統細胞。也可以作為肌肉因子(myokine)。它在肌肉收縮後排入血液,並起到增加脂肪分解和改善胰島素抵抗(insulin resistance)的作用。

炎性細胞因子

炎症由多種可溶性因子介導,包括一組稱為細胞因子的分泌多肽。炎性細胞因子可分為兩類:參與急性炎症的細胞因子和負責任的細胞因子。炎症是組織對損傷的反應,其特徵是在急性期以增加的血流量和血管通透性以及體液,白細胞和炎性介質(例如細胞因子)的積累為特徵。幾種細胞因子在介導急性炎症反應中起關鍵作用,即IL-1,TNF-α,IL-6,IL-11,IL-8和其他趨化因子,G-CSF和GM-CSF。

已知可介導慢性炎症過程的細胞因子可分為參與體液炎症的細胞因子,例如IL-3,IL-4,IL-5,IL-6,IL-7,IL-9,IL-10,IL- 13,轉化生長因子b(TGF-b),以及那些有助於細胞炎症的因子,例如IL-1,IL-2,IL-3,IL-4,IL-7,IL-9,IL-10, IL-12,干擾素(IFN),IFN-g誘導因子(IGIF),TGF-b和TNF-a和-b。炎症異常是一大類疾病,是許多人類疾病的基礎。細胞因子是宿主對感染,免疫反應,炎症和創傷反應的調節劑。一些細胞因子起使疾病惡化的作用(促炎細胞因子),而另一些細胞因子起減輕炎症和促進癒合的作用(抗炎細胞因子)。

產品描述:白介素-6(Interleukin-6, IL-6)ELISA試劑盒採用兩點夾心ELISA(Sandwich ELISA)定量樣品中的白介素-6(Interleukin-6, IL-6)。對白介素-6(Interleukin-6, IL-6)特異的抗體已預先包被在微孔板上。將標準品和样品吸取到孔中,並且存在的任何白介素-6(Interleukin-6, IL-6)都被固定的抗體結合。除去所有未結合的物質後,將HRP-綴合白介素-6(Interleukin-6, IL-6)檢測抗體添加到孔中。洗滌以除去任何未結合的HRP試劑後,將色原溶液添加到孔中,並且根據在初始步驟中結合的白介素-6(Interleukin-6, IL-6)的量成比例地顯色。停止顯色並測量顏色的強度。偵測範圍: 7.5 pg/ml-120 pg/ml, 最低靈敏度0.5 pg/ml可應用於以下樣品來源: Cell culture supernatants, Other biological fluids, Plasma, Serum

LINE群發(RT-9600).jpg

  • Product name

EliKine™ Mouse IL-6 ELISA Kit

  • Reactivity

Mouse

  • Applications

ELISA

  • Detection method

Colorimetric

  • Sample type

Cell culture supernatants, Other biological fluids, Plasma, Serum

  • Assay type

Double antigen

  • Calibration range

7.5 pg/ml-120 pg/ml

  • Limit of detection

0.5 pg/ml

使用注意事項

  • 請勿混用不同試劑盒中的組分,或在試劑盒到期日期之後使用試劑。
  • 打開之前,讓所有試劑至少加室溫30分鐘。
  • 用試劑預清洗移液器吸頭,為每個樣品,標準液和試劑使用新鮮的移液器吸頭,以免污染。
  • 未使用的孔必須在提供的密封袋中於4°C乾燥。
  • 徹底混合對於結果非常重要。建議每10分鐘使用低頻振盪器或稍稍搖動手。
  • 建議所有樣品和標準品一式兩份或一式三份進行測定。

活細胞和死細胞雙染色套組  |  CCK-8細胞增殖和細胞毒性試劑盒  | LDH細胞毒性測定試劑盒 | 細胞衰老檢測試劑盒(β-半乳糖苷酶 | 細胞增殖EdU Image試劑盒  | 細胞核/細胞質分離試劑盒

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人白介素IL-1β(Interleukin 1β)試劑盒 | EliKine™ Human IL-1β ELISA Kit 貨號KET6013  

ABBKINE ELISA KIT 繪圖 (物種).jpg

ABBKINE型錄第一頁_page-0001.jpg

 

細胞因子(Cytokine)功能

細胞因子(Cytokine)具有多種生物學功能。它是急性期反應(acute phase response)的有效誘導劑。在B細胞(B-cells)最終分化為Ig分泌細胞(Ig-secreting cells)的最終分化中起重要作用,參與淋巴細胞(lymphocyte)和單核細胞(monocyte)的分化。它誘導骨髓瘤(myeloma)和漿細胞瘤(plasmacytoma growth)生長,並誘導神經細胞分化(differentiation)。作用於B細胞(B-cells),T細胞(T-cells),肝細胞(hepatocytes),造血祖細胞(hematopoeitic progenitor cells)和CNS中樞神經系統細胞。也可以作為肌肉因子(myokine)。它在肌肉收縮後排入血液,並起到增加脂肪分解和改善胰島素抵抗(insulin resistance)的作用。

炎性細胞因子

炎症由多種可溶性因子介導,包括一組稱為細胞因子的分泌多肽。炎性細胞因子可分為兩類:參與急性炎症的細胞因子和負責任的細胞因子。炎症是組織對損傷的反應,其特徵是在急性期以增加的血流量和血管通透性以及體液,白細胞和炎性介質(例如細胞因子)的積累為特徵。幾種細胞因子在介導急性炎症反應中起關鍵作用,即IL-1,TNF-α,IL-6,IL-11,IL-8和其他趨化因子,G-CSF和GM-CSF。

已知可介導慢性炎症過程的細胞因子可分為參與體液炎症的細胞因子,例如IL-3,IL-4,IL-5,IL-6,IL-7,IL-9,IL-10,IL- 13,轉化生長因子b(TGF-b),以及那些有助於細胞炎症的因子,例如IL-1,IL-2,IL-3,IL-4,IL-7,IL-9,IL-10, IL-12,干擾素(IFN),IFN-g誘導因子(IGIF),TGF-b和TNF-a和-b。炎症異常是一大類疾病,是許多人類疾病的基礎。細胞因子是宿主對感染,免疫反應,炎症和創傷反應的調節劑。一些細胞因子起使疾病惡化的作用(促炎細胞因子),而另一些細胞因子起減輕炎症和促進癒合的作用(抗炎細胞因子)。

白介素-1(Interleukin-1, IL-1)是有史以來最早的細胞因子(cytokines)之一。它的最初發現是可能誘發發燒(fever),控制淋巴細胞(lymphocytes),增加骨髓細胞數量(bone marrow cells)並引起骨關節退化的因素。此時,IL-1以其他幾種名稱為人所知,包括內源性熱原(endogenous pyrogen),淋巴細胞活化因子(lymphocyte activating factor),造血素-1(haemopoetin-1)和單核細胞因子(mononuclear cell factor)等。大約在1984年至1985年間,科學家們證實IL-1實際上由兩種不同的蛋白質組成,現在稱為IL-1α和IL-1β,它們屬於稱為白細胞介素1超家族的細胞因子家族。 IL-1β由巨噬細胞(macrophages),單核細胞(monocytes)和樹突狀細胞(dendritic cells)產生。

IFN-γ(IFN-gamma, 干擾素-γ)是促炎(proinflammatory)細胞因子(cytokine)的原型(prototype),由多種免疫細胞(immune cells)在炎性(inflammatory)條件下產生,特別是由T細胞(T cells)和NK細胞(NK cells)產生。它通過促進Th1細胞的發育(development)和激活(activation),單核細胞(monocytes)和巨噬細胞(macrophage)的化學引誘(chemoattraction)和激活(activation),抗原呈遞分子(antigen presentation molecules)的上調以及B細胞中的免疫球蛋白類別轉換(immunoglobulin class switching),在宿主防禦中起關鍵作用。它還具有抗病毒(antiviral),抗增殖(antiproliferative)和凋亡作用(apoptotic)。另外,IFN-γ通過促進調節性T細胞的發育並抑制Th17細胞分化而充當抗炎介質。 IFN-γ二聚體通過兩個IFN-γR1和兩個IFN-γR2亞基的受體複合物發出信號。

產品描述:EliKine™人白介素IL-1β(Interleukin 1β)試劑盒採用兩點夾心ELISA定量樣品中的白介素IL-1β(Interleukin 1β)。對白介素IL-1β(Interleukin 1β)特異的抗體已預先包被在微板上。將標準品和樣品移入孔中,並且所存在的任何白介素IL-1β(Interleukin 1β)都與固定的抗體結合。除去任何未結合的物質後,將對白介素IL-1β(Interleukin 1β)特異的生物素偶聯抗體添加到孔中。洗滌後,將專有的EliKine™鏈黴親和素-HRP偶聯物添加到孔中。洗滌以除去任何未結合的鏈黴親和素酶試劑後,將底物溶液添加至孔中,並且顏色與初始步驟中結合的白介素IL-1β(Interleukin 1β) 的數量成比例。停止顯色並測量顏色強度。偵測範圍: 7.8 pg/ml-500 pg/ml, 最低靈敏度4 pg/mL可應用於以下樣品來源: Cell culture supernatants, Other biological fluids, Plasma, Serum

Interleukin 1β encoded by IL-1β gene is a member of the interleukin 1 cytokine family. Interleukin 1β is produced by activated macrophages as a proprotein, which is proteolytically processed to its active form by caspase 1 (CASP1/ICE). Interleukin 1β is an important mediator of the inflammatory response, and is involved in a variety of cellular activities, including cell proliferation, differentiation, and apoptosis. The induction of cyclooxygenase-2 (PTGS2/COX2) by Interleukin 1β in the central nervous system (CNS) is found to contribute to inflammatory pain hypersensitivity. IL-1β gene and eight other interleukin 1 family genes form a cytokine gene cluster on chromosome 2.

 

  • Product name

EliKine™ Human IL-1β ELISA Kit

  • Reactivity

Human

  • Applications

ELISA

  • Application notes

EliKine™ Human IL-1β ELISA Kit employs a two-site sandwich ELISA to quantitate IL-1β in samples. An antibody specific for IL-1β has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any IL-1β present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for IL-1β is added to the wells. After washing, proprietary EliKine™ Streptavidin-HRP conjugates is added to the wells. Following a wash to remove any unbound streptavidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of IL-1β bound in the initial step. The color development is stopped and the intensity of the color is measured.

  • Detection method

Colorimetric

  • Sample type

Cell culture supernatants, Other biological fluids, Plasma, Serum

  • Assay type

Sandwich ELISA (quantitative)

  • Assay duration

Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.

  • Kit components
  • Human IL-1β microplate
    • Human IL-1β standard
    • Human IL-1β detect antibody
    • EliKine™ Streptavidin-HRP
    • Standard diluent
    • Assay buffer
    • HRP substrate
    • Stop solution
    • Wash buffer
    • Plate covers.
  • Features & Benefits

EliKine™ Human IL-1β ELISA Kit has high sensitivity and excellent specificity for detection of Human IL-1β. No significant cross-reactivity or interference between Human IL-1β and analogues was observed.

  • Calibration range

7.8 pg/ml-500 pg/ml

  • Limit of detection

4 pg/mL

使用注意事項

  • 請勿混用不同試劑盒中的組分,或在試劑盒到期日期之後使用試劑。
  • 打開之前,讓所有試劑至少加室溫30分鐘。
  • 用試劑預清洗移液器吸頭,為每個樣品,標準液和試劑使用新鮮的移液器吸頭,以免污染。
  • 未使用的孔必須在提供的密封袋中於4°C乾燥。
  • 徹底混合對於結果非常重要。建議每10分鐘使用低頻振盪器或稍稍搖動手。
  • 建議所有樣品和標準品一式兩份或一式三份進行測定。

引用文獻

  1. Evaluation of phage therapy in the treatment of Staphylococcus aureus-induced mastitis in miceH Geng, W Zou, M Zhang. Folia microbiologica, 2019 - Springer
  2. Experimental evidence and network pharmacology identify the molecular targets of Tong Sheng tablets in cerebral ischemia reperfusion injuryTuo Liu, Kangqiang Yang, Guoshun Li, et al. Am J Transl Res 2019;11(6):3301-3316.

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人褪黑激素 (Melatonin ,MT) ELISA 試劑盒 | 貨號KTE61518

產品描述:人褪黑激素 (Melatonin ,MT) ELISA 試劑盒採用兩點夾心ELISA(Sandwich ELISA)定量樣品中的人褪黑激素 (Melatonin ,MT) ELISA 試劑盒。對人褪黑激素 (Melatonin ,MT)特異的抗體已預先包被在微孔板上。將標準品和樣品吸取到孔中,並且存在的任何人褪黑激素 (Melatonin ,MT)都被固定的抗體結合。除去所有未結合的物質後,將HRP-綴合人褪黑激素檢測抗體添加到孔中。洗滌以除去任何未結合的HRP試劑後,將色原溶液添加到孔中,並且根據在初始步驟中結合的人褪黑激素 (Melatonin ,MT) ELISA 試劑盒的量成比例地顯色。停止顯色並測量顏色的強度。偵測範圍: 3.9 pg/mL-250 pg/mL, 最低靈敏度2 pg/ml可應用於以下樣品來源: Cell culture supernatants, Other biological fluids, Plasma, Serum。

ABBKINE ELISA KIT 繪圖.jpg

操作手冊2.jpg

Product name Human Melatonin (MT) ELISA Kit
Reactivity Human
Applications ELISA
Applications notes Human Melatonin (MT) ELISA Kit employs a two-site sandwich ELISA to quantitate Melatonin in samples. An antibody specific for Melatonin has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any Melatonin present is bound by the immobilized antibody. After removing any unbound substances, HRP-Conjugate Human Melatonin detection antibody is added to the wells. Following a wash to remove any unbound HRP reagent, a Chromogen solution is added to the wells and color develops in proportion to the amount of Melatonin bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA
Calibration range 2 ng/L-32 ng/L
Limit of detection 0.1 ng/L

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Product Type   Catalog No Calibration Range Limit of detection
ELISA Assay Kit EliKine™ Human CCL2 ELISA Kit KET6001 62.5 pg/ml-4000 pg/ml 30 pg/mL
ELISA Assay Kit EliKine™ Human CCL3 ELISA Kit KET6002 31.25 pg/mL- 2000 pg/mL 16 pg/Ml
ELISA Assay Kit EliKine™ Human sICAM-1 (CD54) ELISA Kit KET6003 7.81 pg/mL- 500 pg/mL 7.81 pg/mL
ELISA Assay Kit EliKine™ Human CRP ELISA Kit KET6004 15.6 pg/mL-1000 pg/mL 8 pg/mL
ELISA Assay Kit EliKine™ Human EGF ELISA Kit KET6005 3.9 pg/mL-250 pg/mL 2 pg/Ml
ELISA Assay Kit EliKine™ Human bFGF ELISA Kit KET6006 15.6 pg/mL-1000 pg/mL 8 pg/mL
ELISA Assay Kit EliKine™ Human G-CSF ELISA Kit KET6007 31.25 pg/mL-2000 pg/mL 16 pg/mL
ELISA Assay Kit EliKine™ Human GM-CSF ELISA Kit KET6008 11.72 pg/mL-750 pg/mL 5 pg/mL
ELISA Assay Kit EliKine™ Human HGF ELISA Kit KET6009 125 pg/mL-8000 pg/mL 60 pg/mL
ELISA Assay Kit EliKine™ Human IFN-α ELISA Kit KET6010 46.9 pg/mL - 3000 pg/mL 46.9 pg/mL
ELISA Assay Kit EliKine™ Human IFN-γ ELISA Kit KET6011 7.8 pg/mL-500 pg/mL 4 pg/mL
ELISA Assay Kit EliKine™ Human IL-1α ELISA Kit KET6012 7.8 pg/mL-500 pg/mL 4 pg/mL
ELISA Assay Kit EliKine™ Human IL-1β ELISA Kit KET6013 7.8 pg/ml-500 pg/ml 4 pg/mL
ELISA Assay Kit EliKine™ Human IL-2 ELISA Kit KET6014 3.9 pg/ml-250 pg/ml 2 pg/ml
ELISA Assay Kit EliKine™ Human IL-4 ELISA Kit KET6015  3.125 pg/mL- 200 pg/mL 1.5 pg/mL
ELISA Assay Kit EliKine™ Human IL-5 ELISA Kit KET6016 7.8 pg/mL-500 pg/mL 4 pg/mL
ELISA Assay Kit EliKine™ Human IL-6 ELISA Kit KET6017 3.125 pg/ml-200 pg/ml 2 pg/mL
ELISA Assay Kit EliKine™ Human IL-8 ELISA Kit KET6018 3.9 pg/ml-250 pg/ml 2 pg/mL
ELISA Assay Kit EliKine™ Human IL-10 ELISA Kit KET6019 2.35 pg/ml-150 pg/ml 1 pg/mL
ELISA Assay Kit EliKine™ Human IL-12 p70 ELISA Kit KET6020 7.8 pg/mL-500 pg/mL 4 pg/mL
ELISA Assay Kit EliKine™ Human IL-13 ELISA Kit KET6021 7.8 pg/mL-500 pg/mL 4 pg/mL
ELISA Assay Kit EliKine™ Human IL-17 ELISA Kit KET6022 7.8 pg/mL-500 pg/mL 4 pg/mL
ELISA Assay Kit EliKine™ Human IL-18 ELISA Kit KET6023 156 pg/mL- 10000 pg/mL 40 pg/mL
ELISA Assay Kit EliKine™ Human IL-22 ELISA Kit KET6024 15.6 pg/mL-1000 pg/mL 8 pg/mL
ELISA Assay Kit EliKine™ Human IL-23 ELISA Kit KET6025 31.25 pg/mL-2000 pg/mL 16 pg/mL
ELISA Assay Kit EliKine™ Human Leptin ELISA Kit KET6026 31.25 pg/mL- 2000 pg/mL 16 pg/mL
ELISA Assay Kit EliKine™ Human MMP-9 ELISA Kit KET6027 31.25 pg/mL- 2000 pg/mL 16 pg/mL
ELISA Assay Kit EliKine™ Human β-NGF ELISA Kit KET6028 31.25 pg/mL- 2000 pg/mL 16 pg/mL
ELISA Assay Kit EliKine™ Human Resistin ELISA Kit KET6029 31.25 pg/mL-2000 pg/mL 16 pg/mL
ELISA Assay Kit EliKine™ Human TGF-β1 ELISA Kit KET6030 15.6 pg/ml-1000 pg/ml 8 pg/mL
ELISA Assay Kit EliKine™ Human TIMP-1 ELISA Kit KET6031 31.25 pg/ml-2000 pg/ml 16 pg/mL
ELISA Assay Kit EliKine™ Human TNF-α ELISA Kit KET6032 7.8 pg/ml-500 pg/ml 4 pg/mL
ELISA Assay Kit EliKine™ Human VEGF ELISA Kit KET6033 31.25 pg/mL-2000 pg/mL 16 pg/mL
ELISA Assay Kit EliKine™ Human IgG ELISA Kit KET6034 0.125 ng/mL- 8 ng/mL 0.1 ng/mL
ELISA Assay Kit EliKine™ Mouse CCL2 ELISA Kit KET7001 31.25 pg/mL-2000 pg/mL 16 pg/mL
ELISA Assay Kit EliKine™ Mouse GM-CSF ELISA Kit KET7002 7.8 pg/mL-500 pg/mL 4 pg/mL
ELISA Assay Kit EliKine™ Mouse IFN-γ ELISA Kit KET7003 31.25 pg/ml- 2000 pg/ml 16 pg/ml
ELISA Assay Kit EliKine™ Mouse IL-1α ELISA Kit KET7004  7.8 pg/mL-500 pg/mL 4 pg/mL
ELISA Assay Kit EliKine™ Mouse IL-1β ELISA Kit KET7005 15.6 pg/ml-1000 pg/ml 8 pg/mL
ELISA Assay Kit EliKine™ Mouse IL-2 ELISA Kit KET7006 3.13 pg/mL- 200 pg/mL 1.6 pg/mL
ELISA Assay Kit EliKine™ Mouse IL-4 ELISA Kit KET7007 Null Null
ELISA Assay Kit EliKine™ Mouse IL-5 ELISA Kit KET7008 7.8 pg/mL-500 pg/mL 4 pg/mL
ELISA Assay Kit EliKine™ Mouse IL-6 ELISA Kit KET7009 7.82 pg/mL-500 pg/mL 2 pg/mL
ELISA Assay Kit EliKine™ Mouse IL-10 ELISA Kit KET7010 93.75 pg/mL- 6000 pg/mL 50 pg/mL
ELISA Assay Kit EliKine™ Mouse IL-12 p70 ELISA Kit KET7011 31.25 pg/mL- 2000 pg/mL 16 pg/mL
ELISA Assay Kit EliKine™ Mouse IL-17 ELISA Kit KET7012 7.8 pg/mL-500 pg/mL 4 pg/mL
ELISA Assay Kit EliKine™ Mouse IL-22 ELISA Kit KET7013 15.6 pg/mL-1000 pg/mL 8 pg/mL
ELISA Assay Kit EliKine™ Mouse TGF-β1 ELISA Kit KET7014 15.6 pg/ml-1000 pg/ml 8 pg/mL
ELISA Assay Kit EliKine™ Mouse TNF-α ELISA Kit KET7015 15.6 pg/ml-1000 pg/ml 8 pg/mL
ELISA Assay Kit EliKine™ Mouse VEGF ELISA Kit KET7016 15.6 pg/ml-1000 pg/ml 8 pg/ml
ELISA Assay Kit EliKine™ Rat Interferon Gamma (IFN-γ) ELISA Kit KET7017 150 pg/mL-2400 pg/mL 15 pg/mL
ELISA Assay Kit EliKine™ Mouse IgG ELISA Kit KET7018 0.5 ng/mL- 32 ng/mL  0.5 ng/mL
ELISA Assay Kit EliKine™ Rat IL-1β ELISA Kit KET9001 62.5 pg/ml-4000 pg/ml 32 pg/mL
ELISA Assay Kit EliKine™ Rat IL-2 ELISA Kit KET9002 Null Null
ELISA Assay Kit EliKine™ Rat IL-4 ELISA Kit KET9003 1.56 pg/mL-100 pg/mL 1.0 pg/mL
ELISA Assay Kit EliKine™ Rat IL-6 ELISA Kit KET9004 250pg/mL-16000 pg/mL 100 pg/mL
ELISA Assay Kit EliKine™ Rat IL-17 ELISA Kit KET9005 3.13 pg/mL-200 pg/mL 2 pg/mL
ELISA Assay Kit EliKine™ Rat TGF-β1 ELISA Kit KET9006 15.6 pg/mL-1000 pg/mL 8 pg/mL
ELISA Assay Kit EliKine™ Rat TNF-α ELISA Kit KET9007 31.25 pg/mL- 2000 pg/mL 16 pg/ml
ELISA Assay Kit EliKine™ Rat VEGF ELISA Kit KET9008 15.6 pg/ml-1000 pg/ml 8 pg/mL
ELISA Assay Kit EliKine™ Human β-hCG ELISA Kit KET6901 8 IU/L-240 IU/L 2.0 IU/L
ELISA Assay Kit EliKine™ Human LH ELISA Kit KET6902 2 IU/L-75 IU/L 0.5 IU/L
ELISA Assay Kit EliKine™ Human FSH ELISA Kit KET6903 2 IU/L-70 IU/L 1.0 IU/L
ELISA Assay Kit EliKine™ Human Prolactin ELISA Kit KET6904 40 μIU/ml-2000 μIU/ml 50 μIU/ml
ELISA Assay Kit EliKine™ Human TSH ELISA Kit KET6905 0.3 mIU/L-12 mIU/L 0.1 mIU/L
ELISA Assay Kit EliKine™ Testosterone ELISA Kit KET0001 0.1 ng/ml-20 ng/ml 0.1 ng/ml
ELISA Assay Kit EliKine™ Progesterone ELISA Kit KET0002 0.5 ng/ml-30 ng/ml 0.2 ng/ml
ELISA Assay Kit EliKine™ Estradiol ELISA Kit KET0003 40 pg/ml-1500 pg/ml 40 pg/ml
ELISA Assay Kit EliKine™ Free Triiodothyronine (fT3) ELISA Kit KET0004 2 pmol/L-32 pmol/L 2.0 pmol/L
ELISA Assay Kit EliKine™ Free Thyroxine (fT4) ELISA Kit KET0005 4 pmol/L-64 pmol/L 4.0 pmol/L
ELISA Assay Kit EliKine™ Triiodothyronine (T3) ELISA Kit KET0006 0.5 ng/ml-8 ng/ml 0.4 ng/ml
ELISA Assay Kit EliKine™ Thyroxine (T4) ELISA Kit KET0007 20 ng/ml-320 ng/ml 10 ng/ml
ELISA Assay Kit EliKine™ Human PD-1 ELISA Kit KET6035 3.9 pg/mL - 250 pg/mL 3.9 pg/mL
ELISA Assay Kit EliKine™ Human PD-L1 ELISA Kit KET6036 12.5 pg/mL - 800 pg/mL 6.25 pg/mL

 

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斑馬魚GPX1(穀胱甘肽過氧化物酶 1)檢測試劑盒 -ELK Biotechnology貨號ELK8862

Zebrafish GPX1(Glutathione Peroxidase 1) ELISA Kit

ELK KIT.jpg

斑馬魚GPX1(穀胱甘肽過氧化物酶 1)檢測試劑盒 | 貨號ELK8862 (Competitive Inhibition),偵測的靈敏度1.57-100 ng/mL、最低靈敏度0.48 ng/mLELISA原理為利用抗體與抗原之間的高度專一性,做為定量標誌的方法;無論測量抗原或測量抗體都有很高的靈敏度跟專一性。該檢測方法對檢測斑馬魚GPX1(穀胱甘肽過氧化物酶 1具有高靈敏度和出色的特異性ELISA商品的型式多元, 提供三明治ELISA、競爭型ELISA。可應用於serum, plasma, tissue homogenates, cell lysates, cell culture。並在 450nm ± 10nm 的波長下用分光光度計測量顏色變化。然後通過將樣品的 OD 與標準曲線進行比較來確定樣品中斑馬魚GPX1(穀胱甘肽過氧化物酶 1的濃度。

操作手冊2.jpg
ELK Biotechnology公司是一家專注於生命科學、免疫研究的高科技生物企業,位於Biolake, Donghu New & High Technology Development Zone, Wuhan city.ELK Biotechnology是一家專注於免疫產品和分子生物學產品研發和生產的高科技生物公司。產品涵蓋ELISA試劑盒、抗體、基因組提取試劑、分子生物學試劑。涉及免疫化學、腫瘤研究、神經生物學、細胞週期、信號轉導等領域。我們的產品有嚴格的研發和質量檢測流程。每個產品都有完整的研發、生產和質檢記錄。經驗豐富的研發人員,完善的質檢支持部門,嚴格的檢測流程,為客戶提供質量可靠的科研產品。

Product name:

Zebrafish GPX1(Glutathione Peroxidase 1) ELISA Kit

Reactivity:

Zebrafish

Alternative Names:

GSHPX1; Cellular glutathione peroxidase

Assay Type:

Sandwich

Sensitivity:

7.2 pg/mL

Standard:

1000 pg/mL

Range:

15.63-1000 pg/mL

Sample Type:

Serum, plasma and other biological fluids.

Assay Length:

3.5h

Research Area:

Enzyme & Kinase;Metabolic pathway;

Test principle:

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Glutathione Peroxidase 1(GPX1). Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Glutathione Peroxidase 1(GPX1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Glutathione Peroxidase 1(GPX1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Glutathione Peroxidase 1(GPX1) in the samples is then determined by comparing the OD of the samples to the standard curve.

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斑馬魚GSH(穀胱甘肽)檢測試劑盒 -ELK Biotechnology貨號ELK8861

ELK KIT.jpg

Zebrafish GSH(Glutathione) ELISA Kit

原理與知識.jpg

斑馬魚GSH(穀胱甘肽)檢測試劑盒 | 貨號ELK8861 (Competitive Inhibition),偵測的靈敏度1.57-100 ng/mL、最低靈敏度0.48 ng/mLELISA原理為利用抗體與抗原之間的高度專一性,做為定量標誌的方法;無論測量抗原或測量抗體都有很高的靈敏度跟專一性。該檢測方法對檢測斑馬魚GSH(穀胱甘肽)具有高靈敏度和出色的特異性ELISA商品的型式多元, 提供三明治ELISA、競爭型ELISA。可應用於serum, plasma, tissue homogenates, cell lysates, cell culture。並在 450nm ± 10nm 的波長下用分光光度計測量顏色變化。然後通過將樣品的 OD 與標準曲線進行比較來確定樣品中斑馬魚GSH(穀胱甘肽)的濃度。

操作手冊2.jpg

Product name:

Zebrafish GSH(Glutathione) ELISA Kit

Reactivity:

Fish

Alternative Names:

L-Reduced Glutathione

Assay Type:

Competitive Inhibition

Sensitivity:

0.48 ng/mL

Standard:

100 ng/mL

Range:

1.57-100 ng/mL

Sample Type:

serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Assay Length:

2h

Research Area:

Metabolic pathway;

Test principle:

This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Glutathione(GSH) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Glutathione(GSH). Next,Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Glutathione(GSH) in the samples is then determined by comparing the OD of the samples to the standard curve.

 

品牌介紹1-300x68.jpg

ELK Biotechnology公司是一家專注於生命科學、免疫研究的高科技生物企業,位於Biolake, Donghu New & High Technology Development Zone, Wuhan city.ELK Biotechnology是一家專注於免疫產品和分子生物學產品研發和生產的高科技生物公司。產品涵蓋ELISA試劑盒、抗體、基因組提取試劑、分子生物學試劑。涉及免疫化學、腫瘤研究、神經生物學、細胞週期、信號轉導等領域。我們的產品有嚴格的研發和質量檢測流程。每個產品都有完整的研發、生產和質檢記錄。經驗豐富的研發人員,完善的質檢支持部門,嚴格的檢測流程,為客戶提供質量可靠的科研產品。
 

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人 TSH(促甲狀腺激素)檢測試劑盒 -ELK Biotechnology貨號ELK1163

Human TSH(Thyroid Stimulating Hormone) ELISA Kit

ELK KIT.jpg

 

原理與知識.jpg

人 TSH(促甲狀腺激素)檢測試劑盒 | 貨號ELK1163 (Double-antibody Sandwich),偵測的靈敏度0.19-12 uIU/mL、最低靈敏度0.11 uIU/mL。ELISA原理為利用抗體與抗原之間的高度專一性,做為定量標誌的方法;無論測量抗原或測量抗體都有很高的靈敏度跟專一性。該檢測方法對檢測人 TSH(促甲狀腺激素)具有高靈敏度和出色的特異性。ELISA商品的型式多元, 提供三明治ELISA、競爭型ELISA。可應用於serum, plasma, tissue homogenates, cell lysates, cell culture。並在 450nm ± 10nm 的波長下用分光光度計測量顏色變化。然後通過將樣品的 OD 與標準曲線進行比較來確定樣品中人 TSH(促甲狀腺激素)的濃度。

操作手冊2.jpg

Product name:

Human TSH(Thyroid Stimulating Hormone) ELISA Kit

Reactivity:

Human

Alternative Names:

Thyrotropin

Assay Type:

Sandwich

Sensitivity:

0.11 uIU/mL

Standard:

12 uIU/mL

Range:

0.19-12 uIU/mL

Sample Type:

Serum

Assay Length:

3.5h

Research Area:

Endocrinology;Hormone metabolism;

Test principle:

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Thyroid Stimulating Hormone(TSH). Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Thyroid Stimulating Hormone(TSH). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Thyroid Stimulating Hormone(TSH), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Thyroid Stimulating Hormone(TSH) in the samples is then determined by comparing the OD of the samples to the standard curve.

品牌介紹1-300x68.jpg

ELK Biotechnology公司是一家專注於生命科學、免疫研究的高科技生物企業,位於Biolake, Donghu New & High Technology Development Zone, Wuhan city.ELK Biotechnology是一家專注於免疫產品和分子生物學產品研發和生產的高科技生物公司。產品涵蓋ELISA試劑盒、抗體、基因組提取試劑、分子生物學試劑。涉及免疫化學、腫瘤研究、神經生物學、細胞週期、信號轉導等領域。我們的產品有嚴格的研發和質量檢測流程。每個產品都有完整的研發、生產和質檢記錄。經驗豐富的研發人員,完善的質檢支持部門,嚴格的檢測流程,為客戶提供質量可靠的科研產品。

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乾浴加熱水浴槽  |  Finnpipette F3 微量分注器 |  試管振盪器 Vortex Mixer  |  Genereach迷你型微量離心機  |   ELISA儀器

 

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