太鼎生物科技/太鼎食安科技

Organism

Homo sapiens

Source Organ

Bone Marrow

Growth Properties

Adherent

Morphology

Polygonal

Propagation

Grow cells in T25 ECM-coated flasks(G299) with the following conditions. The base medium for this cell line is Prigrow X medium available in ABM, Cat. No. TM100. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (TM999) to a final concentration of 10% and Penicillin/Streptomycin(G255). Atmosphere: air, 95%; Carbon dioxide (CO₂), 5%.

Subculturing

1. Remove and discard culture medium. 2. Add 2.0mL of Trypsin-EDTA solution (TM050) to flask and observe cells under an inverted microscope until cell layer is dispersed. Note: Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. 3. Centrifuge cells at 1500rpm for 3 minutes to pellet. 4. Aspirate out trypsin, leaving pellet undisturbed. 5. Resuspend pellet in fresh culture medium and plate in new culture vessel. 6. Incubate cultures at 37°C . Be sure to use T25 ECM-coated flasks(G299) for growth.

Preservation

1. Freeze Medium: Complete growth medium with 10% DMSO.
2. Storage Temperature: Liquid nitrogen vapour phase.

Notices and Disclaimers

1. ABM products are intended for laboratory research purposes only, unless noted otherwise. They are not intended for use in humans.
2. ABM makes no warranties or representations as to the accuracy of the information on this site. Citations from literature and provided for informational purposes only. ABM does not warrant that such information has been shown to be accurate.

Caution

These Human Normal PV Bone Marrow CD34+ Primary Cells (HNPVBMCD 34C ) are for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information.