太鼎生物科技/太鼎食安科技

Pyruvate Assay Kit

www.biovision.com

Catalog#: K609-100  | Size: 100 assays

【Pyruvate Assay Kit產品介紹】

1. 2種結果判讀: 呈色方式ELISA或是螢光讀值。 Detection method- Absorbance (570 nm) or Fluorescence (Ex/Em 535/587 nm)
2. 樣品型態: 可以是細胞、組織培養液、尿液、血漿、血清或是任何液體來源。Sample type- Cell and Tissue culture supernatants, urine, plasma and serum, as well as many other biological fluids
3. Species reactivity- Mammalian
4. 靈敏度: 1~200uM丙酮酸。Application- The kit detects 1-200 µM pyruvate.

【Pyruvate Assay Kit產品特點】

1. 實驗流程簡單: Simple procedure; takes ~40 minutes
2. 快速且方便: Fast and convenient
3. 套組包括全部分析試劑, 精確測量生物樣品中丙酮酸含量。Kit contains all necessary reagents for accurate measurement of pyruvate concentrations in biological samples

【Pyruvate Assay Kit產品內容物】

• Pyruvate Assay Buffer
• Pyruvate Probe
• DMSO (anhydrous)
• Pyruvate Enzyme Mix
• Pyruvate Standard (100 nmol/µl)

【Pyruvate Assay Kit產品其它介紹】

Pyruvate is a central molecule in metabolism through which sugars enter the citric acid cycle. Pyruvate can be converted to carbohydrates during gluconeogenesis or to fatty acids via acetyl CoA. High levels of pyruvate are associated with liver disease and genetic disorders. Pyruvate has also been used to stimulate metabolism leading to loss of body weight. BioVision provides a simple, direct and automation-ready procedure for measuring pyruvate concentration in various biological samples such as blood, cells, culture and fermentation media, etc. In the assay, pyruvate is oxidized by pyruvate oxidase via enzyme reactions to generate color (λ= 570 nm) and fluorescence (at Ex/Em = 535/587 nm). Since the color or fluorescence intensity is proportional to pyruvate content, the pyruvate concentration can be accurately measured. The kit detects 1-200 µM pyruvate.

Storage Conditions:
-20°C