太鼎生物科技/太鼎食安科技

TC總膽固醇檢測試劑盒 |  Total Cholesterol (TC) Colorimetric Assay Kit貨號KTB2220

CheKine™ Total Cholesterol (TC) Colorimetric Assay Kit-快速檢測血清，血漿，組織，細胞和細菌中總膽固醇(Total Cholesterol, TC)濃度

該試劑盒提供了一種簡單的方法來檢測各種生物樣品（例如血清，血漿，組織，細胞和細菌）中的總膽固醇(Total Cholesterol, TC)濃度。在測定中，酯酶(esterase)催化膽固醇酯(cholesterol esters)的水解，生成游離膽固醇（free cholesterol , FC）和游離脂肪酸（free fatty acids , FFA）。然後膽固醇氧化酶(Cholesterol oxidase)催化游離膽固醇（free cholesterol , FC）產生△4-膽固醇(△4-cholestenone)和過氧化氫(H2O2)。另外，過氧化物酶(peroxidase)可以催化H 2 O 2，4-氨基安替比林(4-aminoantipyrine)和苯酚(phenol)，形成在500nm處具有特徵吸收峰的紅色醌化合物(red quinone compounds)。樣品中的總膽固醇(Total Cholesterol, TC)與獲得的信號成比例。總膽固醇（Total Cholesterol, TC）包括游離膽固醇( free cholesterol )和膽固醇酯(cholesterol esters)。組織總膽固醇（Tissue total cholesterol, TC）是指組織中所有脂蛋白( lipoproteins )所含膽固醇( cholesterol )的總和。請使用500nm ELISA reader檢測; 若需使用分光光度計, 需更換為微量cuvette。

The kit provides a simple method for detecting TC concentration in a variety of biological samples such as serum, plasma, tissues, cells, and bacteria. In the assay, esterase catalyzes the hydrolysis of cholesterol esters to produce free cholesterol (FC) and free fatty acids (FFA). Then Cholesterol oxidase catalyzes free cholesterol (FC) to produce △4-cholestenone and H2O2. Further, H2O2, 4-aminoantipyrine and phenol can be catalyzed by peroxidase to form red quinone compounds which has a characteristic absorption peak at 500 nm. The Total Cholesterol (TC) present in the sample is proportional to the signal obtained.Total cholesterol (TC) includes free cholesterol and cholesterol esters. Tissue total cholesterol (TC) refers to the sum of cholesterol contained in all lipoproteins in the tissue.

客戶自備材料: Materials Required but Not Supplied ·Microplate reader or visible spectrophotometer capable of measuring absorbance at 500 nm ·96-well plate or microglass cuvette, precision pipettes, disposable pipette tips ·Ice maker, refrigerated centrifuge, water bath ·Anhydrous ethanol ·Homogenizer (for tissue samples)。

KTB2220試劑盒可以用於檢測雞蛋樣本，蛋白樣本可直接測定; 蛋黃樣本按操作手冊中，組織樣本處理方法製備即可。(如下所示) 樣品製備注意：建議使用新鮮樣品，如不立即檢測，樣品可在-80℃保存一個月。(一)動物組織：稱取約0.1g組織，加入1mL無水乙醇。在冰上均質化。 4℃ 8,000g 離心 10 分鐘。使用上清液進行測定，並將其置於冰上進行測試。(二) 細胞或細菌：收集 5×10^6.將細胞或細菌放入離心管中，離心後棄上清；加入1mL無水乙醇在冰浴中超聲破碎細胞或細菌5分鐘（功率20%或200W，超聲3s，間隔7s，重複30次）。 4℃ 8,000 g 離心 10 分鐘。使用上清液進行測定，並將其置於冰上進行測試。(三) 血清（血漿）樣品：直接檢測。注：如果以蛋白濃度計算含量，最好用Protein Quantification Kit (BCA Assay), Cat #: KTD3001對總蛋白進行定量。

TC總膽固醇檢測試劑盒 | 貨號KTB2220

CheKine™ Total Cholesterol (TC) Colorimetric Assay Kit

該試劑盒提供了一種簡單的方法來檢測各種生物樣品（例如血清，血漿，組織，細胞和細菌）中的總膽固醇(Total Cholesterol, TC)濃度。在測定中，酯酶催化膽固醇酯(cholesterol esters)的水解，生成游離膽固醇（free cholesterol , FC）和游離脂肪酸（free fatty acids , FFA）。然後膽固醇氧化酶(Cholesterol oxidase)催化游離膽固醇（free cholesterol , FC）產生△4-膽固醇(△4-cholestenone)和過氧化氫(H2O2)。另外，過氧化物酶(peroxidase)可以催化H 2 O 2，4-氨基安替比林(4-aminoantipyrine)和苯酚(phenol)，形成在500nm處具有特徵吸收峰的紅色醌化合物(red quinone compounds)。樣品中的總膽固醇(Total Cholesterol, TC)與獲得的信號成比例。

• Kit components
• TC Working solution
  • Standard
• Features & Benefits
• Determination of Total Cholesterol (TC) content in a variety of biological samples such as serum, plasma, tissues, cells, and bacteria.
  • Detailed sample preparation and result calculation methods are provided.
  • Simple operation steps.
• Usage notes
• Do not mix or substitute reagents or materials from other kit lots or vendors.
  • There are volatile substances in the kit, gloves and masks should be worn during the experiment, and the reagent bottle cap should be tightly closed in time after opening.
  • Ensure all reagents and equipment are at the appropriate temperature before starting the assay.
• Storage instructions

Storage at 4°C and protected from light upon receipt. Kit has a storage time of 12 months form receipt.

• Shipping

Gel pack with blue ice.

活細胞和死細胞雙染色套組  |  CCK-8細胞增殖和細胞毒性試劑盒  | LDH細胞毒性測定試劑盒 | 細胞衰老檢測試劑盒（β-半乳糖苷酶 | 細胞增殖EdU Image試劑盒 

葡萄糖酸呈色分析套組 gluconate acid Assays

3GalT-V; B3GalTx; B3T5; GLCT5; beta3Gal-T5; UDP-Gal:beta-GlcNAc beta-1,3-galactosyltransferase 5; UDP-galactose:beta-N-acetylglucosamine beta-1,3-galactosyltransferase 5

Formulation

Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl.

SEQUENCES The sequence of the target protein is listed below.
NP FKEQSFVYKK DGNFLKLPDT DCRQTPPFLV LLVTSSHKQL AERMAIRQTW GKERMVKGKQ LKTFFLLGTT SSAAETKEVD QESQRHGDII QKDFLDVYYN LTLKTMMGIE WVHRFCPQAA FVMKTDSDMF INVDYLTELL LKKNRTTRFF TGFLKLNEFP IRQPFSKWFV SKSEYPWDRY PPFCSGTGYV FSGDVASQVY NVSKSVPYIK LEDVFVGLCL ERLNIRLEEL HSQPTFFPGG LRFSVCLFRR IVACHFIKPR TLLDYWQALE NSRGEDCPPV

Endotoxin Level <1.0EU per 1µg (determined by the LAL method)

Residues Asn29~Val310 with two N-terminal Tags, His-tag and GST-tag

USAGE Reconstitute in sterile PBS, pH7.2-pH7.4.

STORAGE AND STABILITY Storage: Avoid repeated freeze/thaw cycles. Store at 2-8^oC for one month. Aliquot and store at -80^oC for 12 months.
Stability Test:The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37^oC for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is less than 5% within the expiration date under appropriate storage condition.

About the MARKER (complimentary) Effective Size Range: 10kDa to 70kDa.
Protein bands: 10kDa, 14kDa, 18kDa, 22kDa, 26kDa, 33kDa, 44kDa and 70kDa.
Double intensity bands:The 26kDa, 18kDa, 10kDa bands are at double intensity to make location and size approximation of proteins of interest quick and easy.
Ready-to-use: No need to heat, dilute or add reducing agents before use.

商品如下連結

D-Gluconate (D-Gluconic Acid) Assay Kit (Colorimetric)  

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相關諮詢請洽

台灣代理廠商: 太鼎生物科技有限公司
公司電話: 02-86609496 /0920312382
公司傳真: 02-86609342
公司網站: www.biopioneer.com.tw
連絡業務: 許虹宜 0920312382
連絡信箱: taiding.biotech@msa.hinet.net

膽固醇呈色/螢光分析套組

Cholesterol/Cholesteryl Ester Quantitation Colorimetric/Fluorometric Kit

Sensitive, Colorimetric & Fluorometric Assay



Catalog#: K603-100  | Size: 100 assays

Kit
Summary:

• Detection method- Absorbance (570 nm) or Fluorescence (Ex/Em 535/587 nm)

• Sample type- Cell and Tissue culture supernatants, urine, plasma and serum,
as well as many other biological fluids

• Species reactivity- Mammalian

• Application- The assay detects total cholesterol (cholesterol and cholesteryl
esters) in the presence of cholesterol esterase or free cholesterol in the
absence of cholesterol esterase in the reaction.

Features
& Benefits:

• Simple procedure; takes ~60 minutes

• Fast and convenient

Kit
components:

• Cholesterol Assay Buffer

• Cholesterol Probe (in DMSO, anhydrous)

• Enzyme Mix (lyophilized)

• Cholesterol Esterase (lyophilized)

• Cholesterol Standard (2 µg/µl)

Description:


The Cholesterol/Cholesteryl Ester Quantitation Kit provides a simple method for
sensitive quantification of free cholesterol, cholesteryl esters, or both by
colorimetric or fluorometric methods. Majority of the cholesterol in blood is
in the form of cholesteryl esters which can be hydrolyzed to cholesterol by
cholesterol esterase. Cholesterol is then oxidized by cholesterol oxidase to
yield H₂O₂which
reacts with a sensitive cholesterol probe to produce color (λmax = 570 nm) and
fluorescence (Ex/Em = 535/587 nm). The assay detects total cholesterol
(cholesterol and cholesteryl esters) in the presence of cholesterol esterase or
free cholesterol in the absence of cholesterol esterase in the reaction.
Cholesteryl ester can be determined by subtracting the value of free
cholesterol from the total (cholesterol plus cholesteryl esters).

Glucose葡萄醣呈色分析套組

Glucose Colorimetric Assay Kit II

For samples having reducing substances

Catalog#: K686-100  |
Size: 100 assays

G1P 呈色分析套組

Glucose-1-Phosphate (G1P) Colorimetric Assay Kit

Catalog#: K697-100| Size:100 Assays

葡萄糖-1 - 磷酸（G1P）是葡萄糖代謝和儲存的碳水化合物中，一種重要的中間體。測量細胞內含量，用於分析的碳水化合物代謝途徑和其動力學性質。葡萄糖-1 - 磷酸的測定，G1P轉換為葡萄糖-6 - 磷酸葡萄糖磷酸葡萄糖的存在下，1,6 - 二磷酸，葡萄糖-6 - 磷酸，其後由葡萄糖-6 - 磷酸脫氫酶氧化形成NADH，從而降低了有色產物具有較強的450nm處的吸光度為無色探針。 BioVision的G1P檢測試劑盒快速，靈敏和易於使用及可檢測1μm到10毫米G1P。此G1P檢測試劑盒可用於為多種類型的樣品。

產品資料

Assay
Kit Name:

Glucose-1-Phosphate (G1P) Colorimetric Assay Kit

Kit
Summary:

• Detection method- Absorbance (450 nm)

• Species reactivity- Mammalian

• Applications- The kit can detect glucose-1-phosphate concentrations in the
range of 1 µM – 10 mM .

Sample
Type:

Animal tissues: Liver, muscle and heart etc. Cell culture: Adherent or
suspension cells.

Features
& Benefits:

• Simple procedure; takes ~ 40 minutes

• Rapid, convenient and sensitive

• Kit contains all necessary reagents for accurate measurement of
glucose-1-phosphate levels

Kit
components:

• G1P Assay Buffer

• G1P Enzyme Mix (Lyophilized)

• G1P Developer (Lyophilized)

• G1P Substrate Mix (Lyophilized)

• G1P Standard (Lyophilized)

Description:


Glucose-1-phosphate (G1P) is an important carbohydrate intermediate in glucose
metabolism and storage. In response to hormonal or neural signals,
glycogenolysis occurs in liver and muscle tissues where Glucose-1-phosphate is
released as the rate-limiting step in glycogen breakdown. Glucose-1-phosphate
is subsequently converted to Glucose-6-phosphate by phosphoglucomutase and
enters glycolysis. In glycogen synthesis, glucose is transferred to glycogen
through the actions of Phosphoglucose isomerase (G6P -> G1P),
UDPG-pyrophosphorylase (G1P -> UDP-glucose) and glycogen synthase
(UDP-glucose + glycogen[n] -> UDP + glycogen[n+1]). Glucose-1-phosphate is
found in virtually all organisms from bacteria to higher plants and animals.
Measurement of intracellular G1P levels is crucial for analyzing the
carbohydrate metabolic pathways and their kinetic properties. In
Glucose-1-phosphate assay, G1P is converted to glucose-6-phosphate by phosphoglucomutase
in the presence of Glucose 1,6-biphosphate; glucose-6-phosphate is subsequently
oxidized by glucose-6-phosphate dehydrogenase to form NADH which reduces a
colorless probe to a colored product with strong absorbance at 450 nm.
BioVision’s Glucose-1-phosphate assay kit is rapid, sensitive & easy to use
& can detect 1µM to 10 mM
G1P. This G1P assay kit can be used for a variety of sample types.

Storage
Conditions:

-20°C

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【連絡人】許虹宜 0920-312382 Hung-Yi Hsu

【E-mail】taiding.biotech@msa.hinet.net

【公司電話】02-86609496【公司傳真】02-86609342

【公司網址】www.biopioneer.com.tw

【原廠網址】www.biovision.com

Glycogen 呈色分析套組

Glycogen Colorimetric Assay Kit II

Catalog#: K648-100| Size:100 Assays

產品資料

Assay
Kit Name:

Glycogen Colorimetric Assay Kit II

Kit Summary:

• Detection method- Absorbance (450 nm)

• Applications- The assay can detect less than 4 µg/ml of Glycogen in various
tissues. Analysis of metabolism and cell signaling.

Sample Type:

• Animal tissues: Liver, muscle, etc.

• Cell culture: Adherent or suspension cells.

Features & Benefits:

• Simple, rapid, and high-throughput adaptable.

• Kit contains all necessary reagents for accurate measurement of Glycogen
levels.

Kit Components:

• Glycogen Hydrolysis Buffer

• Gycogen Development Buffer

• Hydrolysis Enzyme Mix (lyophilized)

• Development Enzyme Mix (lyophilized)

• Probe (lyophilized)

• Glycogen Standard (2 mg/ml)

Description:

Glycogen serves as the main carbohydrate storage in animals and can be
converted to glucose readily. It is primarily found in the liver and muscle
tissues. Glycogen is a branched biopolymer comprising of α-1,4 linkage with
α-1,6 linkages occurring every 8-10 glucose units along the backbone. Abnormal
ability to utilize glycogen is found in diabetes and in several genetic
glycogen storage diseases. Biovision’s Glycogen Assay kit II provides a simple,
fast and robust way to measure Glycogen levels in various biological samples.
This assay is suitable for measuring Glycogen levels in samples that contain
reducing substances, which may interfere with the oxidase-based assays. In this
assay, Glycogen is hydrolyzed into glucose, which is oxidized to form an
intermediate that reduces a colorless Probe to a colored product with strong
absorbance at 450 nm. This high-throughput suitable assay kit can detect less
than 4 µg/ml of Glycogen in samples.

Storage Conditions:

-20°C

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Proliferation & Senescence

 Quick Cell Proliferation Assay Kit

 Senescence Detection Kit

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 Bioluminescence Cytotoxicity Assay Kit

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Staining Kit

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Damage & Repair

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Drug Discovery Kits

 HAT Colorimetric Assay Kit & Reagents

 DNA Damage Quantification Kit

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Nitric Oxide Fluorometric & Colorimetric Assay Kits

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Transduction

 cAMP & cGMP Assay Kits

 Akt & JNK Activity Assay Kits

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& Lipid Transfer

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【連絡人】許虹宜 0920-312382 Hung-Yi Hsu

【E-mail】taiding.biotech@msa.hinet.net

【公司電話】02-86609496【公司傳真】02-86609342

【公司網址】www.biopioneer.com.tw

【原廠網址】www.biovision.com

Triglyceride
Quantification Kit三酸甘油酯定量套組, 100個分析, 可應用於組織樣品脂肪定量, 以吸光值或螢光讀值。靈敏度2 pmol~10nmol範圍。

三酸甘油酯定量套組, 訂購貨號Catalog#: K622-100
| Size: 100 assays

【組織樣品準備】

For tissue(~100 mg),cells (~10 million) or other
non-aqueous samples, homogenize in 1 ml solution containing 5 % NP-40 in water,
slowly heat the samples to 80-100 °C in a water bath for 2-5 min or until the
NP-40 becomes cloudy, then cool down to room temperature. Repeat the heating
one more time to solubilize all triglyceride. Centrifuge for 2 min (top speed
using a microcentrifuge) to remove any insoluble material. Dilute

10 fold with
dH2O before the assay.

產品介紹

• Detection method- Absorbance (570 nm) or Fluorescence (Ex/Em 535/587 nm)

• Sample type- Cell and Tissue culture supernatants, urine, plasma and serum,
as well as many other biological fluids

• Species reactivity- Mammalian

• Applications- The kit can detect 2 pmol-10nmol (or 2-10000µM range) of
triglyceride in various samples. The kit also detects monoglycerides and
diglycerides.

產品特點

• Simple procedure; takes ~1 hour

• Fast and convenient

• Kit contains the necessary reagents for accurate measurement of triglyceride
in various biological samples

產品特點

•
Triglyceride Assay Buffer

• TriglycerideProbe (in DMSO, anhydrous)

• Lipase

• TriglycerideEnzyme Mix (lyophilized)

• Triglyceride Standard (1 mM)

Description:


Triglycerides (TG) are the main constituent of vegetable oil, animal fat, LDL
and VLDL, and play an important role as transporters of fatty acids as well as
serving as an energy source. TG are broken down into fatty acids and glycerol,
after which both can serve as substrates for energy producing and metabolic
pathways. High blood levels of TG are implicated in atherosclerosis, heart
disease and stroke as well as in pancreatitis.The Triglyceride Quantification
Kit provides a sensitive, easy assay to measure TG concentration in a variety
of samples. In the assay, TG are converted to free fatty acids and glycerol.
Theglycerol is then oxidized to generate a product which reacts with theprobe
to generate color (spectrophotometry at λ = 570 nm) and fluorescence (Ex/Em =
535/587 nm). The kit can detect 2 pmol-10nmol (or 2-10000µM range) of
triglyceride in various samples. The kit also detects monoglycerides and
diglycerides.

Storage
Conditions:

-20°C

Shipping
Conditions:

gel pack

其它脂肪代謝----相關產品資料