The kit provides a simple method for detecting TC concentration in a variety of biological samples such as serum, plasma, tissues, cells, and bacteria. In the assay, esterase catalyzes the hydrolysis of cholesterol esters to produce free cholesterol (FC) and free fatty acids (FFA). Then Cholesterol oxidase catalyzes free cholesterol (FC) to produce △4-cholestenone and H2O2. Further, H2O2, 4-aminoantipyrine and phenol can be catalyzed by peroxidase to form red quinone compounds which has a characteristic absorption peak at 500 nm. The Total Cholesterol (TC) present in the sample is proportional to the signal obtained.Total cholesterol (TC) includes free cholesterol and cholesterol esters. Tissue total cholesterol (TC) refers to the sum of cholesterol contained in all lipoproteins in the tissue.
The kit provides a simple method for detecting TC concentration in a variety of biological samples such as serum, plasma, tissues, cells, and bacteria. In the assay, esterase catalyzes the hydrolysis of cholesterol esters to produce free cholesterol (FC) and free fatty acids (FFA). Then Cholesterol oxidase catalyzes free cholesterol (FC) to produce △4-cholestenone and H2O2. Further, H2O2, 4-aminoantipyrine and phenol can be catalyzed by peroxidase to form red quinone compounds which has a characteristic absorption peak at 500 nm. The Total Cholesterol (TC) present in the sample is proportional to the signal obtained.
Alternative
TC; cholesterol
Kit components
• TC Working solution
• Standard
Features & Benefits
• Determination of Total Cholesterol (TC) content in a variety of biological samples such as serum, plasma, tissues, cells, and bacteria.
• Detailed sample preparation and result calculation methods are provided.
• Simple operation steps.
Usage notes
• Do not mix or substitute reagents or materials from other kit lots or vendors.
• There are volatile substances in the kit, gloves and masks should be worn during the experiment, and the reagent bottle cap should be tightly closed in time after opening.
• Ensure all reagents and equipment are at the appropriate temperature before starting the assay.
Storage instructions
Storage at 4°C and protected from light upon receipt. Kit has a storage time of 12 months form receipt.
Shipping
Gel pack with blue ice.
Precautions
The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background
Total cholesterol (TC) includes free cholesterol and cholesterol esters. Tissue total cholesterol (TC) refers to the sum of cholesterol contained in all lipoproteins in the tissue.
Alternative
TC; cholesterol
客戶自備材料: Materials Required but Not Supplied ·Microplate reader or visible spectrophotometer capable of measuring absorbance at 500 nm ·96-well plate or microglass cuvette, precision pipettes, disposable pipette tips ·Ice maker, refrigerated centrifuge, water bath ·Anhydrous ethanol ·Homogenizer (for tissue samples)。
The kit provides a simple method for detecting TC concentration in a variety of biological samples such as serum, plasma, tissues, cells, and bacteria. In the assay, esterase catalyzes the hydrolysis of cholesterol esters to produce free cholesterol (FC) and free fatty acids (FFA). Then Cholesterol oxidase catalyzes free cholesterol (FC) to produce △4-cholestenone and H2O2. Further, H2O2, 4-aminoantipyrine and phenol can be catalyzed by peroxidase to form red quinone compounds which has a characteristic absorption peak at 500 nm. The Total Cholesterol (TC) present in the sample is proportional to the signal obtained.
Alternative
TC; cholesterol
Kit components
TC Working solution
• Standard
Features & Benefits
Determination of Total Cholesterol (TC) content in a variety of biological samples such as serum, plasma, tissues, cells, and bacteria.
• Detailed sample preparation and result calculation methods are provided.
• Simple operation steps.
Usage notes
Do not mix or substitute reagents or materials from other kit lots or vendors.
• There are volatile substances in the kit, gloves and masks should be worn during the experiment, and the reagent bottle cap should be tightly closed in time after opening.
• Ensure all reagents and equipment are at the appropriate temperature before starting the assay.
Storage instructions
Storage at 4°C and protected from light upon receipt. Kit has a storage time of 12 months form receipt.
Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl.
SEQUENCES The sequence of the target protein is listed below.
NP FKEQSFVYKK DGNFLKLPDT DCRQTPPFLV LLVTSSHKQL AERMAIRQTW GKERMVKGKQ LKTFFLLGTT SSAAETKEVD QESQRHGDII QKDFLDVYYN LTLKTMMGIE WVHRFCPQAA FVMKTDSDMF INVDYLTELL LKKNRTTRFF TGFLKLNEFP IRQPFSKWFV SKSEYPWDRY PPFCSGTGYV FSGDVASQVY NVSKSVPYIK LEDVFVGLCL ERLNIRLEEL HSQPTFFPGG LRFSVCLFRR IVACHFIKPR TLLDYWQALE NSRGEDCPPV
Endotoxin Level<1.0EU per 1µg (determined by the LAL method)
ResiduesAsn29~Val310 with two N-terminal Tags, His-tag and GST-tag
USAGEReconstitute in sterile PBS, pH7.2-pH7.4.
STORAGE AND STABILITYStorage: Avoid repeated freeze/thaw cycles. Store at 2-8oC for one month. Aliquot and store at -80oC for 12 months.
Stability Test:The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37oC for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is less than 5% within the expiration date under appropriate storage condition.
About the MARKER (complimentary)Effective Size Range: 10kDa to 70kDa.
Protein bands: 10kDa, 14kDa, 18kDa, 22kDa, 26kDa, 33kDa, 44kDa and 70kDa.
Double intensity bands:The 26kDa, 18kDa, 10kDa bands are at double intensity to make location and size approximation of proteins of interest quick and easy.
Ready-to-use: No need to heat, dilute or add reducing agents before use.
• Sample type- Cell and Tissue culture supernatants, urine, plasma and serum, as well as many other biological fluids
• Species reactivity- Mammalian
• Application- The assay detects total cholesterol (cholesterol and cholesteryl esters) in the presence of cholesterol esterase or free cholesterol in the absence of cholesterol esterase in the reaction.
Features & Benefits:
• Simple procedure; takes ~60 minutes
• Fast and convenient
Kit components:
• Cholesterol Assay Buffer
• Cholesterol Probe (in DMSO, anhydrous)
• Enzyme Mix (lyophilized)
• Cholesterol Esterase (lyophilized)
• Cholesterol Standard (2 µg/µl)
Description:
The Cholesterol/Cholesteryl Ester Quantitation Kit provides a simple method for sensitive quantification of free cholesterol, cholesteryl esters, or both by colorimetric or fluorometric methods. Majority of the cholesterol in blood is in the form of cholesteryl esters which can be hydrolyzed to cholesterol by cholesterol esterase. Cholesterol is then oxidized by cholesterol oxidase to yield H₂O₂which reacts with a sensitive cholesterol probe to produce color (λmax = 570 nm) and fluorescence (Ex/Em = 535/587 nm). The assay detects total cholesterol (cholesterol and cholesteryl esters) in the presence of cholesterol esterase or free cholesterol in the absence of cholesterol esterase in the reaction. Cholesteryl ester can be determined by subtracting the value of free cholesterol from the total (cholesterol plus cholesteryl esters).
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• Sample type- Cell and Tissue culture supernatants, urine, plasma and serum, as well as many other biological fluids, fermentation media, food samples etc.
• Species reactivity- Mammalian
• Application- The kit can detect glucose concentrations in the range of 20µM – 10mM .
Features & Benefits:
• Simple procedure; takes ~ 30 minutes to complete the assay
• Fast and convenient
Kit components:
• Glucose Assay Buffer
• Glucose Substrate Mix
• Glucose Enzyme Mix
• Glucose Standard ( 100 mM )
Description:
Glucose is an important fuel source to generate the universal energy molecule ATP. Serum glucose level is a key diagnostic parameter for many metabolic disorders. BioVision’s Glucose Assay Kit II provides direct measurement of glucose in various biological samples (e.g., serum, plasma, other body fluids, food, growth media, etc.). In this assay, glucose is specifically oxidized to generate a product which reacts with a dye to generate color (λ = 450 nm) whose intensity is proportional to glucose concentration. The method is rapid, simple, sensitive, and suitable for high throughput. This assay is particularly suitable for serum and urine samples since it is unaffected by reducing substances which can interfere with other suppliers offering oxidase-based kits. The assay is also suitable for monitoring glucose level during fermentation and glucose feeding in protein expression processes. The kit can detect glucose concentrations in the range of 20µM – 10mM .
Storage Conditions:
-20°C
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• Applications- The kit can detect glucose-1-phosphate concentrations in the range of 1 µM – 10 mM .
Sample Type:
Animal tissues: Liver, muscle and heart etc. Cell culture: Adherent or suspension cells.
Features & Benefits:
• Simple procedure; takes ~ 40 minutes
• Rapid, convenient and sensitive
• Kit contains all necessary reagents for accurate measurement of glucose-1-phosphate levels
Kit components:
• G1P Assay Buffer
• G1P Enzyme Mix (Lyophilized)
• G1P Developer (Lyophilized)
• G1P Substrate Mix (Lyophilized)
• G1P Standard (Lyophilized)
Description:
Glucose-1-phosphate (G1P) is an important carbohydrate intermediate in glucose metabolism and storage. In response to hormonal or neural signals, glycogenolysis occurs in liver and muscle tissues where Glucose-1-phosphate is released as the rate-limiting step in glycogen breakdown. Glucose-1-phosphate is subsequently converted to Glucose-6-phosphate by phosphoglucomutase and enters glycolysis. In glycogen synthesis, glucose is transferred to glycogen through the actions of Phosphoglucose isomerase (G6P -> G1P), UDPG-pyrophosphorylase (G1P -> UDP-glucose) and glycogen synthase (UDP-glucose + glycogen[n] -> UDP + glycogen[n+1]). Glucose-1-phosphate is found in virtually all organisms from bacteria to higher plants and animals. Measurement of intracellular G1P levels is crucial for analyzing the carbohydrate metabolic pathways and their kinetic properties. In Glucose-1-phosphate assay, G1P is converted to glucose-6-phosphate by phosphoglucomutase in the presence of Glucose 1,6-biphosphate; glucose-6-phosphate is subsequently oxidized by glucose-6-phosphate dehydrogenase to form NADH which reduces a colorless probe to a colored product with strong absorbance at 450 nm. BioVision’s Glucose-1-phosphate assay kit is rapid, sensitive & easy to use & can detect 1µM to 10 mM G1P. This G1P assay kit can be used for a variety of sample types.
Storage Conditions:
-20°C
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• Applications- The assay can detect less than 4 µg/ml of Glycogen in various tissues. Analysis of metabolism and cell signaling.
Sample Type:
• Animal tissues: Liver, muscle, etc.
• Cell culture: Adherent or suspension cells.
Features & Benefits:
• Simple, rapid, and high-throughput adaptable.
• Kit contains all necessary reagents for accurate measurement of Glycogen levels.
Kit Components:
• Glycogen Hydrolysis Buffer
• Gycogen Development Buffer
• Hydrolysis Enzyme Mix (lyophilized)
• Development Enzyme Mix (lyophilized)
• Probe (lyophilized)
• Glycogen Standard (2 mg/ml)
Description:
Glycogen serves as the main carbohydrate storage in animals and can be converted to glucose readily. It is primarily found in the liver and muscle tissues. Glycogen is a branched biopolymer comprising of α-1,4 linkage with α-1,6 linkages occurring every 8-10 glucose units along the backbone. Abnormal ability to utilize glycogen is found in diabetes and in several genetic glycogen storage diseases. Biovision’s Glycogen Assay kit II provides a simple, fast and robust way to measure Glycogen levels in various biological samples. This assay is suitable for measuring Glycogen levels in samples that contain reducing substances, which may interfere with the oxidase-based assays. In this assay, Glycogen is hydrolyzed into glucose, which is oxidized to form an intermediate that reduces a colorless Probe to a colored product with strong absorbance at 450 nm. This high-throughput suitable assay kit can detect less than 4 µg/ml of Glycogen in samples.
Storage Conditions:
-20°C
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For tissue(~100 mg),cells (~10 million) or other non-aqueous samples, homogenize in 1 ml solution containing 5 % NP-40 in water, slowly heat the samples to 80-100 °C in a water bath for 2-5 min or until the NP-40 becomes cloudy, then cool down to room temperature. Repeat the heating one more time to solubilize all triglyceride. Centrifuge for 2 min (top speed using a microcentrifuge) to remove any insoluble material. Dilute
• Sample type- Cell and Tissue culture supernatants, urine, plasma and serum, as well as many other biological fluids
• Species reactivity- Mammalian
• Applications- The kit can detect 2 pmol-10nmol (or 2-10000µM range) of triglyceride in various samples. The kit also detects monoglycerides and diglycerides.
產品特點
• Simple procedure; takes ~1 hour
• Fast and convenient
• Kit contains the necessary reagents for accurate measurement of triglyceride in various biological samples
產品特點
• Triglyceride Assay Buffer
• TriglycerideProbe (in DMSO, anhydrous)
• Lipase
• TriglycerideEnzyme Mix (lyophilized)
• Triglyceride Standard (1 mM)
Description:
Triglycerides (TG) are the main constituent of vegetable oil, animal fat, LDL and VLDL, and play an important role as transporters of fatty acids as well as serving as an energy source. TG are broken down into fatty acids and glycerol, after which both can serve as substrates for energy producing and metabolic pathways. High blood levels of TG are implicated in atherosclerosis, heart disease and stroke as well as in pancreatitis.The Triglyceride Quantification Kit provides a sensitive, easy assay to measure TG concentration in a variety of samples. In the assay, TG are converted to free fatty acids and glycerol. Theglycerol is then oxidized to generate a product which reacts with theprobe to generate color (spectrophotometry at λ = 570 nm) and fluorescence (Ex/Em = 535/587 nm). The kit can detect 2 pmol-10nmol (or 2-10000µM range) of triglyceride in various samples. The kit also detects monoglycerides and diglycerides.
Application & Usage: Western blotting (0.5-4 µg/ml) and Immunohistochemistry (10-20 µg/ml, frozen & paraffin). Recombinant GST-GSK-3α fusion protein (Cat.#: 7003-100) can be used as a positive control. However, the optimal conditions should be determined individually.
Storage Temp.: -20 °C
Shipping: gel pack
Background Descriptions: Glutathione S-transferase (GST) is a widely used protein fusion partner, since it provides an easily detectable Tag and also a simple purification process with little effect on the biological function of the protein of interest.
Handling: The antibody solution should be gently mixed before use.
Usage: For Research Use Only! Not to be used in humans.
Kit Summary: • Detection method- Absorbance (450 nm) • Sample type- Serum • Species reactivity- Mammalian • Applications-This human Visfatin ELISA Kit is an enzyme-linked immunosorbent assay (ELISA) for quantitative determination of visfatin. Works well with rat samples.
Features & Benefits: • Simple procedure • Fast and convenient
Kit components: • Antibody coated 96-well plate (12 x 8 well strips, with absorbed monoclonal antibody to human visfatin) • 5X Wash concentrate, 100 ml • 5X Diluent, 50 ml (for reagent dilution) • 1X Secondary antibody, 12 ml (Rabbit Polyclonal antibody against human visfatin) • 100X Detector, 150 µl (HRP conjugated anti-rabbit IgG) • Standard, recombinant human visfatin (16 ng), lyophilized • QC sample= 2 positive control of recombinant human visfatin (3 ng/ml, 0.5 ng/ml) • Substrate, 12 ml (chromogenic reagent) • Stop solution, 12 ml ( 1 M H3PO4) • Plate sealer (3 sealers)
Description: Visfatin, an adipocytokine that is highly enriched in the visceral fat of both humans and mice and whose expression level in plasma increases during the development of obesity. Visfatin corresponds to pre-B cell colony-enhancing factor (PBEF), a 52-kD cytokine expressed in lymphocytes. PBEF is an inflammatory cytokine that plays a requisite role in the delayed neutrophil apoptosis of sepsis. Visfatin exerted insulin-mimetic effects in cultured cells and lowered plasma glucose levels in mice. It was found that visfatin binds to and activates the insulin receptor. Plasma level of visfatin in patients with type 2 diabetes mellitus was elevated, suggesting that measurement of plasma visfatin provides a relevant tool for understanding metabolic diseases.
Hydroxyproline (4-hydroxyproline, 4-羥基脯胺酸) is a common nonproteinogenic amino acid. It is found only in collagen and elastin in mammals but exists in a number of other proteins in plants. Hydroxyproline is formed only as a post-translational modification in the peptide chain and proline hydroxylase does not hydroxylate free proline. Hydroxyproline in tissue hydrolysates is a direct measure of the amount of collagen or gelatin present. A variety of disease states are believed to affect collagen turnover and can cause elevated serum or urine hydroxyproline. Such conditions range from neoplastic, inflammatory, renal or bone disease to endocrine and autoimmune disorders. BioVision’s Hydroxyproline Assay Kit is designed to measure hydroxyproline in tissue or protein/peptide hydrolysates. It can be used to measure hydroxyproline from other biological samples such as serum or urine if they have undergone a prior purification process. It is an easy, convenient method which results in a chromogen with an absorbance maximum at 560 nm. The assay is useful over the range of 0.1-2 μg.
Kit Summary: • Detection method- Absorbance (560 nm) • Sample type- Cell and Tissue culture supernatants, urine, plasma and serum, as well as many other biological fluids • Species reactivity- Mammalian • Applications- The assay is useful over the range of 0.1-2 μg.
Features & Benefits: • Simple procedure; takes less than ~100 minutes • Convenient and easy to use
Kit components: • Oxidation Buffer • Chloramine T Concentrate • Perchloric acid/Isopropanol Solution • DMAB Concentrate (in DMSO) • Hydroxyproline Standard (1 mg/ml)
Storage Conditions: -20°C
Shipping Conditions: gel pack
一般操作注意事項:
Store the kit at +4°C and protect from light. Please read the entire protocol before performing the assay. The reagent concentrates are stable as supplied. Once the concentrates have been diluted to working concentration, they are only good for 2-3 hr so only make as enough each reagent as necessary for the number of samples and standards to be quantified.
Kit Summary: • Detection method- Absorbance (450 nm) • Sample type- Cell and Tissue culture supernatants, urine, plasma and serum, as well as many other biological fluids • Species reactivity- Mammalian • Applications- This human Adiponectin ELISA Kit is an enzyme-linked immunosorbent assay (ELISA) for quantitative determination of adiponectin in human serum, plasma or various tissue or cell culture supernatants
Features & Benefits: • Simple procedure • Fast and convenient
Kit components: • Antibody coated 96-well plate (12 x 8-well strips) • 5X Wash concentrate, 100 ml • 5X Diluent, 50 ml • Secondary antibody, 12 ml • 100X Detector, 150 µl • Standard, recombinant human adiponectin (64 ng), 1 vial, lyophilized • QC sample= positive control having 7-11 µg/ml human adiponectin, 1 vial, lyophilized • Substrate I, 6 ml • Substrate II, 6 ml • Stop solution, 12 ml
Description: Adipose tissue secretes a number of biologically active soluble factors (collectively named adipocytokines) that regulate glucose and fatty acid metabolism. Measurement of serum adiponectin levels gives us important information on the role of adiponectin in regulation of glucose and/or lipid metabolism. This human Adiponectin ELISA Kit is an enzyme-linked immunosorbent assay (ELISA) for quantitative determination of adiponectin in human serum, plasma or various tissue or cell culture supernatants. In the assay, monoclonal antibody specific for human adiponectin has been pre-coated onto 96 well microplate. Standards and samples are pipetted into the wells and adiponectin present is bound by immobilized antibody. The bound adiponectin is then captured by anti-human adiponectin polyclonal antibody. With HRP conjugated anti-rabbit IgG and a HRP substrate, the colors developed in proportion to the bound adiponectin, can be easily measured by Elisa plate reader.
Kit Summary: • Detection method- Absorbance (570 nm) or Fluorescence (Ex/Em 535/587 nm) • Sample type- Cell and Tissue culture supernatants, urine, plasma and serum, as well as many other biological fluids • Species reactivity- Mammalian • Applications-The kit can detect 0.001 – 10 mM Creatine.
Features & Benefits: • Simple procedure; takes ~ 1 hour • Fast and convenient • Kit contains all necessary reagents for accurate measurement of Creatine
Description: Creatine is an endogenous compound whose function is to maintain a high ATP/ADP ratio, by way of its phosphorylated form and creatine kinase. Creatine supplementation has been used in the treatment of muscular, neurological and neurodegenerative diseases, as well as a sport performance enhancer. Detection of creatine level has wide applications in research and development. BioVision’s Creatine Assay Kit provides an accurate, convenient measure of creatine in a variety of biological samples. In the assay, creatine is enzymatically converted to sarcosine which is then specifically oxidized to generate a product that converts a colorless probe to an intensely red color (λmax = 570 nm), and highly fluorescent (Ex/Em = 538/587 nm) product. Creatine is therefore easily detected by either colorimetric or fluorometric methods. Detection range 0.001 – 10 mM Creatine.
*Note: Sarcosine gives background for the assay. For samples which may contain a significant amount of sarcosine, do a background control. Prepare a reaction without the creatinase (replacing the creatinase with 2 μl assay buffer).
(5) Measure O.D. 570 nm for colorimetric assay or fluorescence at
Ex/Em = 535/590 nm in a micro-plate reader.
(6)計算fatty acid含量
Published References:
1) Bobrovnikova-Marjon E et al (2008) PNAS; 105: 16314 - 16319.
Kit Summary: • Detection method- Absorbance (570 nm) or Fluorescence (Ex/Em 535/587 nm) • Sample type- Cell and Tissue culture supernatants, urine, plasma and serum, as well as many other biological fluids • Species reactivity- Mammalian • Application- The kit has a detection limit 2 µM free fatty acid in variety samples.
Features & Benefits: • Simple procedure; takes ~40 minutes • Fast and convenient • Kit contains all necessary reagents for rapid, sensitive and accurate measurement of Adipolysis in cell culture samples
Description: Fatty Acids play very important roles in normal metabolism and many disease developments. They are precursors to a number of bioactive classes of compounds such as prostaglandins, leucotrienes and others, and have been implicated in diverse functions such as autism, immune system and inflammation response. BioVision’s Free Fatty Acid Quantification Kit provides a convenient, sensitive enzyme-based method for detecting the long-chain free fatty acids in various biological samples, such as serum, plasma and other body fluids, food, growth media, etc. In the assay, Fatty Acids are converted to their CoA derivatives, which are subsequently oxidized with the concomitant generation of color or fluorescence. C-8 (octanoate) and longer fatty acids can then be easily quantified by either colorimetric (spectrophotometry at λ = 570 nm) or fluorometric (at Ex/Em = 535/587 nm) methods with detection limit 2 µM free fatty acid in variety samples.
Kit Summary: • Detection method- Absorbance (570 nm) or Fluorescence (Ex/Em 535/587 nm) • Sample type- Cell and Tissue culture supernatants, urine, plasma and serum, as well as many other biological fluids • Species reactivity- Mammalian • Applications- The kit can detect 0.001 -10 mM of various Lactate samples.
Features & Benefits: • Simple procedure; takes ~40 minutes • Fast and convenient • Kit contains the necessary reagents for accurate measurement of Lactate colorimetrically and fluorometrically
Kit components:
• Lactate Assay Buffer • Lactate Probe (in DMSO) • Lactate Enzyme Mix • L(+)-Lactate Standard (100 nmol/µl)
Description: Abnormal high concentration of lactate has been related to disease states such as diabetes and lactate acidosis, etc. L(+)-Lactate is the major stereo-isomer of lactate formed in human intermediary metabolism and is present in blood. D(-)-Lactate is also present but only at about 1-5% of the concentration of L(+)-Lactate. In the Lactate Assay Kit, lactate specifically reacts with a enzyme mix to generate a product, which interacts with lactate probe to produce color (570 nm) and fluorescence (at Ex/Em = 535/587 nm). The kit provides a convenient means for detecting L(+)-Lactate in biological samples such as in blood circulation, in cells, in culture mediums, in fermentation mediums, etc. There is no need of pretreatment or purification of samples. The kit can detect 0.001 -10 mM of various Lactate samples.
Kit Summary: • Detection method-Absorbance (532 nm) or Fluorescence (Ex/Em 532/553 nm) • Sample type- Cell and Tissue culture supernatants, plasma and other biological fluids (optimized by end user). • Species reactivity- All • Applications- The kit detects MDA levels as low as 1 nmol/well colorimetrically and 0.1 nmol/well fluorometrically.
Features & Benefits: • Simple procedure • Fast and convenient • Sensitive assay for measuring lipid peroxidation (LPA) in a wide range of samples
Description: Quantification of lipid peroxidation is essential to assess oxidative stress in pathophysiological processes. Lipid peroxidation forms Malondialdehyde (MDA) and 4-hydroxynonenal (4-HNE), as natural bi-products. Measuring the end products of lipid peroxidation is one of the most widely accepted assays for oxidative damage. BioVision’s Lipid Peroxidation Assay Kit provides a convenient tool for sensitive detection of the MDA in a variety of samples. The MDA in the sample is reacted with Thiobarbituric Acid (TBA) to generate the MDA-TBA adduct. The MDA-TBA adduct can be easily quantified colorimetrically (λ = 532 nm) or fluorometrically (Ex/Em = 532/553 nm). This assay detects MDA levels as low as 1 nmol/well colorimetrically and 0.1 nmol/well fluorometrically.
Kit Summary: • Detection method- Absorbance (570 nm) or Fluorescence (Ex/Em 535/587 nm) • Sample type- Cell and Tissue culture supernatants, urine, plasma and serum, as well as many other biological fluids and growth medium • Species reactivity- Mammalian • Applications- The kit detects 1-10000 µM glucose samples.
Features & Benefits: • Simple procedure; takes ~40 minutes • Fast and convenient • Kit provides all necessary buffers and reagents for a accurate assay of glucose in various mammalian samples.
Description: Glucose (C₆H₁₂O₆; FW: 180.16) is a very important fuel source to generate universal energy molecules ATP. Glucose level is a key diagnostic parameter for many metabolic disorders. Measurement of glucose can be very important in both research and drug discovery processes. The Glucose Assay Kit provides direct measurement of glucose in various biological samples (e.g., serum, plasma, body fluid, food, growth medium, etc.). Glucose Enzyme Mix specifically oxidizes glucose to generate a product which reacts with a dye to generate color (λ = 570 nm) and fluorescence (Ex/Em = 535/587 nm). The generated color and fluorescence is proportionally to the glucose amount. The method is rapid, simple, sensitive, and suitable for high throughput. The assay is also suitable for monitoring glucose level during fermentation and glucose feeding in protein expression processes. The kit detects 1-10000 µM glucose samples.
1. 2種結果判讀: 呈色方式ELISA或是螢光讀值。 Detection method- Absorbance (570 nm) or Fluorescence (Ex/Em 535/587 nm) 2. 樣品型態: 可以是細胞、組織培養液、尿液、血漿、血清或是任何液體來源。Sample type- Cell and Tissue culture supernatants, urine, plasma and serum, as well as many other biological fluids 3. Species reactivity- Mammalian 4. 靈敏度: 1~200uM丙酮酸。Application- The kit detects 1-200 µM pyruvate.
【Pyruvate Assay Kit產品特點】
1. 實驗流程簡單: Simple procedure; takes ~40 minutes 2. 快速且方便: Fast and convenient 3. 套組包括全部分析試劑, 精確測量生物樣品中丙酮酸含量。Kit contains all necessary reagents for accurate measurement of pyruvate concentrations in biological samples
Pyruvate is a central molecule in metabolism through which sugars enter the citric acid cycle. Pyruvate can be converted to carbohydrates during gluconeogenesis or to fatty acids via acetyl CoA. High levels of pyruvate are associated with liver disease and genetic disorders. Pyruvate has also been used to stimulate metabolism leading to loss of body weight. BioVision provides a simple, direct and automation-ready procedure for measuring pyruvate concentration in various biological samples such as blood, cells, culture and fermentation media, etc. In the assay, pyruvate is oxidized by pyruvate oxidase via enzyme reactions to generate color (λ= 570 nm) and fluorescence (at Ex/Em = 535/587 nm). Since the color or fluorescence intensity is proportional to pyruvate content, the pyruvate concentration can be accurately measured. The kit detects 1-200 µM pyruvate.
Lipid Peroxidation (MDA) Assay Kit
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