Viagen DirectPCR® DNA Extraction System 太鼎生物科技有限公司代理 DirectPCR Lysis Reagent (mouse tail)尾巴gDNA萃取 @ 太鼎生物科技/太鼎食安科技

萃取gDNA很簡單

傳統萃取 “尾巴gDNA”，無法直接PCR；因組織內含有PCR反應抑制因子，抑制PCR反應。須經由有機溶劑或kit萃取gDNA。“專利研發” DirectPCR Lysis Reagents具有抑制PCR抑制物的反應，只要一個步驟反應，即可將DNA由組織中釋放，並且不須經由任何純化的步驟，溶液可以直接PCR，進行genotyping分析。 最經濟的萃取試劑, 500次尾巴, 只須6250元 ( 0.5c m尾巴加入200ul為例 )

「Genotyping基因型鑑定」是全面搜尋人類致病基因的重要分析技術。DirectPCR® DNA Extraction System，可以

快速有效萃取動物組織, 尾巴基因型DNA (genomic DNA)，不須經由Phenol/Chloroform萃取步驟, 不須再通過column

等繁瑣步驟純化，只要一個步驟，效率100%。如此方便快速的方法, 可以應用於尾巴genomic DNA萃取、細胞

genomic DNA萃取、耳朵genomic DNA萃取、卵黃囊genomic DNA萃取。

更多資料: http://www.viagenbiotech.com/

DirectPCR DNA Extraction Reagents Genotyping without DNA isolation
Description: Viagen DirectPCR® DNA Extraction System is a single-tube system for rapid preparation of DNA from mouse tails, ear pieces, yolk sac, and culture cells. The patent-pending components developed by scientists at Viagen Biotech Inc. allow that the resulting DNA extracts are compatible with genomic PCR for genotyping. (more) Simplified procedure: 1. Lyse tails in DirectPCR reagents. 2. Incubate for 45 min at 85°C . 3. PCR using 1 µl lysates. Detailed protocols: Tail, Ear, Yolk Sac, Cultured cells. Representative PCR results DirectPCR Products: Tail, Ear, Yolk Sac, Cultured cells.

DirectPCR DNA Extraction Reagents	Cat#	Comments
DirectPCR Lysis Reagent (mouse tail)	[101-T]	250 mouse tails (50 ml)
DirectPCR Lysis Reagent (mouse tail)	[102-T]	500 mouse tails (100 ml)
DirectPCR Lysis Reagent (yolk sac)	[201-Y]	yolk sacs (50 ml)
DirectPCR Lysis Reagent (yolk sac)	[202-Y]	yolk sacs (100 ml)
DirectPCR Lysis Reagent (cell)	[301-C]	cultured cells (50 ml)
DirectPCR Lysis Reagent (cell)	[302-C]	cultured cells (100 ml)
DirectPCR Lysis Reagent (mouse ear)	[401-E]	mouse ear (25 ml)
DirectPCR Lysis Reagent (mouse ear)	[402-E]	mouse ear (50 ml)
Proteinase K Solution	[501-PK]	US and Canada only