太鼎生物科技/太鼎食安科技

高遷移率族蛋白 1 (HMGB1) ELISA 試劑盒 | Cloud clone貨號SEA399Hu

ELISA Kit for High Mobility Group Protein 1 (HMGB1)

高遷移率族蛋白 1 (HMGB1) ELISA 試劑盒 (酶聯免疫吸附試驗法) | 貨號SEA399Hu，偵測的靈敏度62.5-4,000pg/mL、最低靈敏度28.3pg/mL。ELISA原理為利用抗體與抗原之間的高度專一性，做為定量標誌的方法；無論測量抗原或測量抗體都有很高的靈敏度跟專一性。Cloud-Clone corp提供高靈敏度之ELISA (酵素免疫分析套組/酶聯免疫吸附試驗法)，專門生產研究用檢測試劑的生技公司，於美國研發和生產，產品線包括ELISA Kit、CLIA Kit、Antibody及recombinant protein；ELISA產品系列超過6000種以上，物種齊全包括rat、mouse、rabbit、cavia、human、porcine、bovine、simian等多物種商品。品質保證。ELISA商品的型式多元， 提供三明治ELISA、競爭型ELISA、高靈敏度設計ELISA。Uncoated ELISA型式；包括Capture Antibody, Detection Antibody、Standard、Streptavidin-HRP、TMB Substrate, 96-well Plate。

Specificity

This assay has high sensitivity and excellent specificity for detection of High Mobility Group Protein 1 (HMGB1).
No significant cross-reactivity or interference between High Mobility Group Protein 1 (HMGB1) and analogues was observed.

Recovery

Matrices listed below were spiked with certain level of recombinant High Mobility Group Protein 1 (HMGB1) and the recovery rates were calculated by comparing the measured value to the expected amount of High Mobility Group Protein 1 (HMGB1) in samples.

Reagents and materials provided

Assay procedure summary

1. Prepare all reagents, samples and standards;
   2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
   3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
   4. Aspirate and wash 3 times;
   5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
   6. Aspirate and wash 5 times;
   7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
   8. Add 50µL Stop Solution. Read at 450nm immediately.

高遷移率族蛋白 1 (HMGB1) 相關之抗體 | 蛋白質 | ELISA | CLIA產品