【Blood PCR Kit---不需萃取全血DNA】訂購貨號#G463。Bloodirect DNA Polymerase。可應用於各哺乳動物之血液PCR，包括鳥類之血液PCR－太鼎生物科技/太鼎食安科技

全血直接PCR-----不需萃取DNA

血液中大量存在 PCR inhibitors(抑制劑)，PCR inhibitors抑制PCR polymerase反應，因為無法直接由全血中進行PCR反應。傳統全血PCR，全血需經由DNA萃取，才能進行PCR反應。Abmgood應用Error-Prone
PCR技術，研發Bestag DNA Polymerase，克服PCR inhibitors之干擾，客戶僅將血液加入Bloodirect DNA
Polymerase，即可進行PCR反應。(PCR反應液需額外購買)

若需Bloodirect
2x MasterMix 資料如下所示:

http://tw.myblog.yahoo.com/taiding2000/article?mid=1077&prev=-1&next=1076

Cat.No.:	G 463 G 462
Quantity:	400 U (400 μl) 100 U (400 μl)

產品特點

1. 全血不需萃取DNA之步驟，即可進行PCR反應。

2. 20% (v/v)全血反應體積，不需前處理血液樣品

3. 血液樣品可來自新鮮血液、冷凍血液、EDTA保存之全血、FTA Elute Card之全血、Guthrie Cards之全血、或flter paper之全血。

4. 可應用於各哺乳動物之血液PCR，包括鳥類之血液PCR

5. 降低污染之可能性

產品介紹

Bestaq DNA
Polymerase使用Error-Prone PCR技術，效能高，靈敏度佳。Bloodirect 2X PCR Mastermix 包含Bloodirect DNA
Polymerase, dNTPs, 及 buffer 。Ready-to-use混合液。.客戶直接使用全血，即可進行PCＲ反應。排除DNA萃取之需求。全血之使用量，可佔PCR反應體積之20%。在EDTA、Citrate、及10 % (v/v) heparin以下之存在，皆不受影響PCR之反應。乾血sample (Guthrie cards or 903 cards (Whatman, NJ))，取1 mm punch disc，即可直接PCR反應。

產品內容物

訂購貨號#G462----- (25個反應，反應體積50ul)

Bloodirect DNA Polymerase (1
U/μl) 100 μl

5X Bloodirect Buffer, with
Mg2+ 1 ml

訂購貨號#G463-----(100個反應，反應體積50ul)

Bloodirect DNA Polymerase (1
U/μl) 400 μl

5X
Bloodirect Buffer, with Mg2+ 2ml

buffer內容物

Storage
Buffer Components

50 mM Tris-HCl (pH 8.0), 100 mM NaCl, 0.1 mM EDTA, 5
mM DTT, 50 % glycerol and 1 % Triton®X-100.

Mg離子濃度建議

Magnesium
Concentration

Final [1X] Bloodirect Buffer contains 3.0 mM Magnesium Sulfate, total Magnesium
Sulfate concentration of up to 6.0 mM
can be used. If necessary, Magnesium concentration can also be lowered with
EDTA solution to eliminate non-specific amplification.

產品資料內容

General Notes

1. Whole blood samples containing sodium citrate, sodium
EDTA, potassium EDTA or sodium heparin are recommended.

2. Blood volume higher than 10 % (v/v) would increase the
difficulties in recovering aqueous supernatant from blood cell debris after
PCR.

3. Dry blood stored on Guthrie cards or 903 cards ( Whatman , NJ )
can be used directly by adding 1 mm
punch disc to PCR reaction

4. For blood stored on FTA cards ( Whatman , NJ ),
first incubate a 1 mm punch
disc in 50 μl of water at 55oC for 5 minutes, then
discard the water and use the disc directly in PCR reaction.

5. Up to 10 % (v/v) DMSO can be used for high-GC targets

Concentration	1 U/μl
Storage	Store all components at -20°C in a non-frost-free freezer.
Notes	1. Whole blood samples containing sodium citrate, sodium EDTA, potassium EDTA, or sodium heparin are recommended. 2. Blood volume higher than 10 % (v/v) would increase the difficulties in recovering aqueous supernatant from blood cell debris that remains after PCR. 3. Dry blood stored on Guthrie cards or 903 cards ( Whatman , NJ ) can be used directly by adding 1 mm punch disc to PCR reaction 4. For blood stored on FTA cards ( Whatman , NJ ), first incubate a 1 mm punch disc in 50 μl of water at 55°C for 5 minutes, then discard the water and use the disc directly in PCR reaction. 6. Up to 10% (v/v) DMSO can be used for high-GC targets.