全血直接PCR-----不需萃取DNA





血液中大量存在 PCR inhibitors(抑制劑)PCR inhibitors抑制PCR polymerase反應,因為無法直接由全血中進行PCR反應。傳統全血PCR,全血需經由DNA萃取,才能進行PCR反應。Abmgood應用Error-Prone
PCR
技術,研發Bestag DNA Polymerase,克服PCR inhibitors之干擾,客戶僅將血液加入Bloodirect DNA
Polymerase
,即可進行PCR反應。(PCR反應液需額外購買)





若需Bloodirect
2x MasterMix
資料如下所示:  




http://tw.myblog.yahoo.com/taiding2000/article?mid=1077&prev=-1&next=1076




 












Cat.No.:




G 463            G 462




Quantity:




400 U (400 μl)    100 U (400 μl)







產品特點



1.     全血不需萃取DNA之步驟,即可進行PCR反應。


2.     20% (v/v)全血反應體積,不需前處理血液樣品


3.     血液樣品可來自新鮮血液、冷凍血液、EDTA保存之全血、FTA Elute Card之全血、Guthrie Cards之全血、或flter paper之全血。


4.     可應用於各哺乳動物之血液PCR,包括鳥類之血液PCR


5.     降低污染之可能性






產品介紹





 





Bestaq DNA
Polymerase
使用Error-Prone PCR技術,效能高,靈敏度佳。Bloodirect 2X PCR Mastermix 包含Bloodirect DNA
Polymerase, dNTPs,
buffer Ready-to-use混合液。.客戶直接使用全血,即可進行PCR反應。排除DNA萃取之需求。全血之使用量,可佔PCR反應體積之20%。在EDTACitrate、及10 % (v/v) heparin以下之存在,皆不受影響PCR之反應。乾血sample (Guthrie cards or 903 cards (Whatman, NJ)),取1 mm punch disc,即可直接PCR反應。





 





 


產品內容物





訂購貨號#G462----- (25個反應,反應體積50ul)





Bloodirect DNA Polymerase (1
U/μl)  100 μl  





5X Bloodirect Buffer, with
Mg2+     1 ml   





訂購貨號#G463-----(100個反應,反應體積50ul)





Bloodirect DNA Polymerase (1
U/μl)  400 μl  





5X
Bloodirect Buffer, with Mg2+     2ml  





buffer內容物





Storage
Buffer Components





50 mM Tris-HCl (pH 8.0), 100 mM NaCl, 0.1 mM EDTA, 5
mM DTT, 50 % glycerol and 1 % Triton
®X-100.





Mg離子濃度建議





Magnesium
Concentration





Final [1X] Bloodirect Buffer contains 3.0 mM Magnesium Sulfate, total Magnesium
Sulfate concentration of up to 6.0 mM
can be used. If necessary, Magnesium concentration can also be lowered with
EDTA solution to eliminate non-specific amplification.





產品資料內容





General Notes





1. Whole blood samples containing sodium citrate, sodium
EDTA, potassium EDTA or sodium heparin are recommended.





2. Blood volume higher than 10 % (v/v) would increase the
difficulties in recovering aqueous supernatant from blood cell debris after
PCR.





3. Dry blood stored on Guthrie cards or 903 cards ( Whatman , NJ )
can be used directly by adding 1 mm
punch disc to PCR reaction





4. For blood stored on FTA cards ( Whatman , NJ ),
first incubate a 1 mm punch
disc in 50 μl of water at 55
oC for 5 minutes, then
discard the water and use the disc directly in PCR reaction.





5. Up to 10 % (v/v) DMSO can be used for high-GC targets





 



















Concentration




1 U/μl




Storage




Store all
components at -20°C in a
non-frost-free freezer.




Notes




1. Whole
blood samples containing sodium citrate, sodium EDTA, potassium EDTA, or
sodium heparin are recommended.

2. Blood volume higher than 10 % (v/v) would increase the difficulties in
recovering aqueous supernatant from blood cell debris that remains after PCR.

3. Dry blood stored on Guthrie cards or 903 cards ( Whatman , NJ )
can be used directly by adding 1 mm
punch disc to PCR reaction

4. For blood stored on FTA cards ( Whatman ,
NJ ), first incubate a 1 mm punch disc in 50 μl of water at 55°C for 5 minutes, then
discard the water and use the disc directly in PCR reaction.

6. Up to 10% (v/v) DMSO can be used for high-GC targets.






 







 


【供應廠商】太鼎生物科技有限公司



【連絡人】許虹宜 0920-312382



【台北公司】02-86609496



【相關網址】 www.biopioneer.com.tw



【原廠網址】http://www.abmgood.com







 


 

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