太鼎生物科技/太鼎食安科技

品牌abmgood 貨號LV999 規格100ML

Speedy Lentivirus Purification

  Recombinant lentiviral vectors have been shown to be a powerful
tool for stable gene transfer to both dividing and non-dividing cells in vitro
and in vivo.Through years of experience with lentiviral vectors, ABM has
developed its own proprietary pLenti-combo packaging mix and an efficient
protocol for rapid production of recombinant lentiviral vectors with titers up
to 10⁷IU/ml. The quickest and easiest way to purify and concentrate
lentiviral particles up to 100x. The concentrated viral preparations can be
used for in vivo injection or like applications that require high titer of
viral particles.

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Advantages of ABM's Lentiviral Expression
Vectors

1. stable plasmid; rare plasmid DNA re-arrangement

2. very high yielding plasmid

3. no special competent cells required

4. His and HA tag compatible

5. ABM is THE source for your lentiviral expression needs!

Lentivirus Purification Protocol Cat. No.: LV999

1. Harvest viral supernatant, spin at 2500g for 10 minutes and filter through a
0.45 um syringe filter.

2. Add 5ml of Lenti-binding solution to 45
ml of the viral supernatant and mix thoroughly by inversion.

3. Centrifuge at >= 5000g at 4C for 10 minutes.

4. Decant the supernatant. Be careful not
to disturbe the pellet.

5. Resuspend the viral pellet in 0.45ml ~
4.5ml of serum-containing medium, depending on the concentration of virus
needed.

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【代理廠商】: 太鼎生物科技有限公司

【公司電話】: 02-86609496 傳真 : 02-86609342
【公司網站】: www.biopioneer.com.tw
【連絡業務】: 許虹宜 0920312382
【連絡信箱】: taiding.biotech@msa.hinet.net