【Lentivirus慢病毒表現、純化】 @ 太鼎生物科技/太鼎食安科技

Oct 04 Tue 2022 16:15
PEG病毒力價濃縮套組 | 貨號K904-200 (5倍200個反應) /ab102538

PEG病毒力價濃縮套組 | 貨號K904-200 (5倍200個反應) /ab102538

PEG Virus Precipitation Kit

產品描述：病毒通常以低力價產生。Biovision提供低速簡單之方法，濃縮病毒力價和去除雜質，。PEG病毒沉澱試劑盒簡單，方便和省時，可以將病毒濃縮而無需超離心。該試劑盒可用於小實驗室樣品或大規模病毒製備，產量高，病毒滴度高。該試劑盒可用於將細胞培養基或環境樣品中的逆轉錄病毒(retroviruses)，桿狀病毒(baculoviruses)，慢病毒(baculoviruses)和噬菌體(lentiviruses)等進行濃縮。病毒可以集中在100倍以上。根據病毒類型和來源，提供優化的病毒再懸浮液，使病毒恢復最大化達40-100％。整個過程使用無毒試劑，濃縮病毒可用於感染，病毒DNA或RNA純化等應用。Virus was usually produced at a low titer. It often needs to be concentrated for storage or further applications. A quick, easy and inexpensive method is desired to concentrate virus and remove impurities. BioVision’s PEG Virus Precipitation Kit provides an easy, convenient and time-saving method to concentrate virus without ultra-centrifugation. The kit can be used for small lab samples or large scale virus preparation with high yield and high viral titer. The kit can be used to concentrate retroviruses, baculoviruses, lentiviruses, and phages etc. in cell culture medium or environmental samples. Virus can be concentrated over 100 folds. An optimized Virus Re-suspension Solution is provided to maximize viral recovery by 40-100% depending on the virus type and sources. The whole process uses non-toxic reagents. The concentrated virus can be used for infection, viral DNA or RNA purification, etc.

PEG (聚乙二醇) 病毒力價濃縮套組 |PEG Virus Precipitation Kit 貨號K904-200/ab102538

PEG病毒濃縮套組

PEG Virus Precipitation Kit PEG病毒濃縮產品，有效提高病毒力價。可應用於Lentivirus 病毒濃縮，登革熱病毒 (40~50nm)、流感病毒(~100nm)、腸病毒(20~30nm)之病毒顆粒之濃縮。應用於細胞培養之病毒液濃縮、環境sample病毒的濃縮等應用。

PEG病毒濃縮產品特點:

不須超高速離心
安全無毒性，濃縮完之病毒可直接感染細胞，及進行下游分析
可將病毒液濃縮10 至100 倍，回收率40~100%
適用於 Lentivirus、Phages、登革熱病毒、流感病毒、腸病毒之病毒顆粒之等病毒濃縮。
包裝為5 倍無菌濃縮液，整組可純化500ml/2000ml病毒液 (50或200reaction, k904-50/K904-200)

Applications	The kit can be used for small lab samples or large scale virus preparation with high yield and high viral titer.
Features & Benefits	• A quick, easy and inexpensive method is desired to concentrate virus and remove impurities • Easy, convenient and time-saving method to concentrate virus without ultra-centrifugation • Virus can be concentrated over 100 folds • The whole process uses non-toxic reagents
Kit Components	• PEG Solution (5X) • Virus Re-suspension Solution (1X)

引用文獻

Chanyang Kim, et al., (2018) IGF-1 protects SH-SY5Y cells against MPP+-induced apoptosis via PI3K/PDK-1/Akt pathway, Endocrine Connections, May 2018, 29459421

Andrews et al., High Preexisting Serological Antibody Levels Correlate with Diversification of the Influenza Vaccine Response. J. Virol., Mar 2015; 89: 3308 - 3317.

Lee et al., First Report of Oryctes rhinoceros nudivirus (Coleoptera: Scarabaeidae) Causing Severe Disease in Allomyrina dichotoma in Korea. J Insect Sci, Mar 2015; 15: 26.

Warnes et al., Inactivation of Murine Norovirus on a Range of Copper Alloy Surfaces Is Accompanied by Loss of Capsid Integrity. Appl. Envir. Microbiol., Feb 2015; 81: 1085 - 1091.

Busskamp et al., Rapid neurogenesis through transcriptional activation in human stem cells. Mol Syst Biol, Nov 2014; 10: 760.

最新消息!! 太鼎食安科技(bpfood)-成立新網站

https://www.bpfood.com.tw/

太鼎生物科技有限公司成立於生物科技始在台灣嶄露頭角之初，以提供學術單位最專業、最先進技術的實驗設備為我們的核心任務。成立至今，我們憑藉著累積而來的，是有關於自身的專業、經驗的累積，以及大環境的波折損益，一直到目前對於生物科技界能夠有所瞭解，太鼎兢兢業業的態度未曾懈怠。

你的食安太鼎把關！

太鼎是食品安全檢測設備的專業供應商，擁有實驗室等級的分子生物、光學實驗儀器，免疫分析研究領域所需的試劑和設備，技術領先、品質穩定、產品設備齊全。太鼎食安科技是食品安全檢測設備的專，提供3M Petrifilm 檢測試藥: 食品微生物檢測、大腸桿菌檢測、大腸菌群檢測、李氏特菌、3M Petrifilm 金黃色葡萄球菌、3M Petrifilm 沙門氏桿菌檢測等快檢片、ATP微生物冷光儀、水質微生物檢測。擁有實驗室等級的分子生物、光學實驗儀器，免疫分析研究領域所需的試劑和設備，技術領先、品質穩定、產品設備齊全。

我們的專業團隊能幫助研究單位計算出精確的數據、臨床檢測的及時需要，提供客戶專業的服務，是太鼎最大的一直以此為職志，並且自豪於此。太鼎的SLOGAN是：「我們或許不是最龐大的供應商，但我們希望成為你最可靠的及時雨」。

太鼎以追求專業自期，就如大鼎具深而見廣。太鼎將盡其所能，提供生物科技界眾多先輩後進之需求，為學術發展和良性互動貢獻一份力量。

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個人分類：【Lentivirus慢病毒表現、純化】

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Jul 13 Wed 2016 18:35
【TBX18－GFP Adenovirus】

TBX18－GFP Adenovirus（人類）

品牌abmgood/貨號#375888A/ 包裝250ul (病毒力價1x10⁶pfu/mL)各力價濃縮加購如下附件

TBX18-GFP Adenovirus (Human)

Service	Description	Cat. No.
Adenovirus Purification	Amplify and purify (2-3 ml of 10⁹ pfu/ml)	C070
	Amplify and purify (2-3 ml of 10¹⁰ pfu/ml)	C071
	Amplify and purify (5 x 200 μl of 10¹¹ pfu/ml)	C072
	Amplify and purify (5 x 200 μl of 10¹² pfu/ml)	C005

TBX18－GFP Adenovirus產品應用

This adenovirus is part of abm’s Adenoviral Expression System and can be used directly to transiently over-express your gene of interest in a wide range of host cells. This adenovirus can be used to amplify more adenovirus by transducing HEK293 cells. For enhanced transduction efficiency, the use of ViralPlus (G698) at 1:100 is recommended at the time of transduction.

Vector	pAdenoG-IRES-GFP
Vector Size	37302bp
Promoter	CMV
Insert	TBX18
Insert Size	1824 bp
Organism	Human
Accession #	BC132715
Chromosome	6Q14.1-Q15
Tags	GFP reporter (IRES)
Gene Type	Wild Type. Mutants can be constructed via custom services. Please refer to our custom service page or contact our technical support for more info.
Titer	1x10⁶pfu/mL
Storage Buffer	DMEM with 2.5% glycerol
Shipping & Storage	Adenoviruses are stable at room temperature for short term storage and are shipped with gel ice packs. Do not store at -20°C or 4°C for long term. For long term storage, it is recommended to store the viruses at -80°C and avoid repeated freeze-thaw cycles. Before storing at -80°C, it is advisable to make small aliquots to avoid repeated freeze-thaw cycles. Screw cap vials are recommended to decrease chances of the storage buffer desiccating which leads to decrease in infectivity. Diluting the virus is not recommended.
Disclaimer	1. Disclaimer for transcript variants: The provided accession number refers to the transcript (mRNA) sequence for this product. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. All sales are final. 2. The provided accession number refers to the transcript (mRNA) sequence for this product. Please verify that this is the desired transcript sequence by cross-referencing. This is important because a single gene can have multiple different transcripts owing to naturally occurring variations. All sales are final. 3. Disclaimer for intended use: All of abm's vectors and viral particles are for research use ONLY and NOT for therapeutic/diagnostic applications. abm is not liable for any repercussions arising from the use of its vector(s) in therapeutic/diagnostic application(s). 4. Disclaimer for extra nucleotides: Cloning may lead to the insertion of extra nucleotides at the 5' or 3' end of the gene of interest which, in most cases, is innocuous to the stability/functionality and the expression of that gene.
Guarantee	abm guarantees that the correct ORF construct is provided and the mRNA expression is displayed upon successful transduction. If this is not the case, we will provide a one-time replacement. Customers must provide adequate data to show >80% transfection efficiency with a positive control, plus additional qPCR data or a western blot to evaluate the level of gene expression. The replacement will not be covered by the same guarantee. Please note that due to the large number of variables applicable, any further expression analysis (e.g. protein expression) is not covered by the guarantee, as such analysis is dependent on the end user's experimental conditions.

引用文獻

Das, A et al. "Engulfment of apoptotic cells by macrophages: a role of microRNA-21 in the resolution of wound inflammation.." J Immunol 192(3):1120-9 (2014). DOI: 10.4049/jimmunol.1300613. PubMed: 24391209 . Application: Transduction.

Hai, B et al. "Transient activation of hedgehog pathway rescued irradiation-induced hyposalivation by preserving salivary stem/progenitor cells and parasympathetic innervation.." Clin Cancer Res 20(1):140-50 (2014). DOI: 10.1158/1078-0432.CCR-13-1434. PubMed: 24150232 . Application: Transduction.

Reineke, EL et al. "Steroid Receptor Coactivator-2 is a Dual Regulator of Cardiac Transcription Factor Function." J. Biol. Chem 289 (25):17721-17731 (2014). DOI: 10.1074/jbc.M113.539908. PubMed: 24811170. Application: Adenovirus Expression.

Gomes, AP et al. "Declining NAD(+) induces a pseudohypoxic state disrupting nuclear-mitochondrial communication during aging." Cell 155(7):1624-38 (2013). DOI: 10.1016/j.cell.2013.11.037. PubMed: 24360282 . Application: Transduction.

Hoffman, BE et al. "Development and characterization of a human articular cartilage-derived chondrocyte cell line that retains chondrocyte phenotype.." J Cell Physiol. 222(3):695-702 (2010). DOI: 10.1002/jcp.21994. PubMed: 20020445 . Application: Adenovirus Transduction Protocol; Transduction.

更多相關諮詢請洽

台灣代理廠商: 太鼎生物科技有限公司

公司電話: 02-86609496 /0920312382
公司傳真: 02-86609342
公司網站: www.biopioneer.com.tw
連絡業務: 許虹宜 0920312382
連絡信箱: taiding.biotech@msa.hinet.net

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個人分類：【Lentivirus慢病毒表現、純化】

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microRNA(miRNA)是一類有大約22個核苷酸組成的非編碼小分子RNA，其廣泛存在於真核生物中。miRNA在個體發育的不同時期及不同組織中有不同的表達模式，在發育和分化中具有重大的調控作用。miRNA的檢測方法主要有Northern Blot 等基於分子雜交的方法，這些方法敏感度低、耗時長、RNA的用量較大。應用Real-Time PCR技術來對miRNA進行檢測，具有快速、特異性強、靈敏度高等優點。Abmgood提供miRNA QPCR偵測套組，一系列產品包括有miRNA primer、universal primer、Evagreen Mastermix等。各種microRNA表現與偵測的產品；其種類包括有miRNA表現載體、miRNA分離與偵測套組，另提供更多研究miRNA的資料來源。　　

ABM miRNA表現載體

GFP miRNA Lentivirus/Vector
miRNA Lentivirus/Vector
iCumate miRNA Lentivectors
miRNA(microRNA) Adenovirus
miRNA Inhibitor Lentivirus/Vector
miRNA Inhibitor Adenovirus
3'UTR miRNA Reporter & Cell Line

miRNA萃取與偵測

: G269／G270 miRNA cDNA Synthesis Kit Work Flow－ABMgood

產品型號	產品名稱	包裝
G269 G270	miRNA cDNA Synthesis Kit	25 Reactions 100 Reactions
Human miRNA internal control primer
MPH00001	U6-2 Primers	150ul/10uM
MPH00003	SNORD44 Primers	150ul/10uM
MPH00004	SNORD47 Primers	150ul/10uM
MPH00005	SNORD48 Primers	150ul/10uL

<更多相關諮詢請洽>

【代理廠商】: 太鼎生物科技有限公司

【公司電話】: 02-86609496 傳真 : 02-86609342
【公司網站】: www.biopioneer.com.tw
【連絡業務】: 許虹宜 0920312382
【連絡信箱】: taiding.biotech@msa.hinet.net

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個人分類：【Lentivirus慢病毒表現、純化】

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Jul 05 Tue 2016 00:03
【30分/ 低速病毒濃縮套組】Abmgood#LV999。Speedy Lentivirus Purification。

品牌abmgood 貨號LV999 規格100ML

Speedy Lentivirus Purification

Recombinant lentiviral vectors have been shown to be a powerful
tool for stable gene transfer to both dividing and non-dividing cells in vitro
and in vivo.Through years of experience with lentiviral vectors, ABM has
developed its own proprietary pLenti-combo packaging mix and an efficient
protocol for rapid production of recombinant lentiviral vectors with titers up
to 10⁷IU/ml. The quickest and easiest way to purify and concentrate
lentiviral particles up to 100x. The concentrated viral preparations can be
used for in vivo injection or like applications that require high titer of
viral particles.

-----------------------------------------------

Advantages of ABM's Lentiviral Expression
Vectors

1. stable plasmid; rare plasmid DNA re-arrangement

2. very high yielding plasmid

3. no special competent cells required

4. His and HA tag compatible

5. ABM is THE source for your lentiviral expression needs!

Lentivirus Purification Protocol Cat. No.: LV999

1. Harvest viral supernatant, spin at 2500g for 10 minutes and filter through a
0.45 um syringe filter.

2. Add 5ml of Lenti-binding solution to 45
ml of the viral supernatant and mix thoroughly by inversion.

3. Centrifuge at >= 5000g at 4C for 10 minutes.

4. Decant the supernatant. Be careful not
to disturbe the pellet.

5. Resuspend the viral pellet in 0.45ml ~
4.5ml of serum-containing medium, depending on the concentration of virus
needed.

<更多相關諮詢請洽>

【代理廠商】: 太鼎生物科技有限公司

【公司電話】: 02-86609496 傳真 : 02-86609342
【公司網站】: www.biopioneer.com.tw
【連絡業務】: 許虹宜 0920312382
【連絡信箱】: taiding.biotech@msa.hinet.net

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個人分類：【Lentivirus慢病毒表現、純化】

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Jul 04 Mon 2016 23:45
【低速病毒純化套組】Lentivirus病毒純化、30分鐘內快速純化病毒。濃縮效果約100x

ABM Speedy Lentivirus Purification kit 病毒快速純化套組

傳統的病毒純化方法，大多利用超高速離心(ultracentrifuge)，搭配濃度梯度(CsCl)等方法進行病毒分離，需要純熟技術且過程費時。病毒純化套組可免除使用超高速離心，更簡單、快速取得高濃度病毒液。

30分鐘內快速純化病毒。
可達濃縮效果約100x。

: 293 cells 感染純化前（A）與純化後（B）的lentivirus 48hr後的結果

----------------------------------------------

ABM qPCR Lentivirus Titration(Titer) Kit

One-Step qPCR，2小時內可完成定量。
Primer經特殊設計，解決primer dimer干擾。
附有病毒濃度標準液兩組，準確定量病毒濃度。
搭配ABM qPCR Mastermix，效果更佳。

ABM_lentiviral_titer_workflow

--------------------------------------------

產品編號	產品名稱	包裝
LV999	Speedy Lentivirus Purification	100ml
LV900	qPCR Lentivirus Titration(Titer) Kit	100 Reactions
其他病毒純化相關產品
LV998	Ultra-Pure Lentivirus Purification Kit－可純化病毒濃度達10¹¹病毒顆粒	1 Kit
G171 G172 G173	PuRetro™ Lentivirus Purification Kit	2 Standard Units 5 Standard Units 2 Giga Units

---------------------------------------------------------------

<原廠資料連結>

<更多相關諮詢請洽>

【代理廠商】: 太鼎生物科技有限公司

【公司電話】: 02-86609496 傳真 : 02-86609342
【公司網站】: www.biopioneer.com.tw
【連絡業務】: 許虹宜 0920312382
【連絡信箱】: taiding.biotech@msa.hinet.net

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個人分類：【Lentivirus慢病毒表現、純化】

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Jul 03 Sun 2016 15:15
【Lentivirus病毒定量-快篩片】定量lentiviral capsule protein; 靈敏度可達1×105 IU/mL病毒濃度

Lentivirus病毒定量-快篩片

Instant Lentivirus Titration Card慢病毒定量卡使用橫向流方法（lateral flow immunoassay）偵測lentiviral capsule protein進行定量。比起傳統infection based assays需3-4天，Instant Lentivirus Titration Card 只要10分鐘即可得到定量結果。

簡單操作，10分鐘獲得結果－不需額外萃取步驟
目測觀察結果顏色變化
用途：評估培養液中適當的病毒濃度
靈敏度可達1×10⁵ IU/mL病毒濃度（或7.7 pg/ml的p24 viral capsid protein）

ABM_Lenti-Card-figure

產品編號	產品名稱	包裝
G614 G615	Instant Lentivirus Titration Card	10 Tests 20 Tests
病毒純化／定量相關產品
LV999	Speedy Lentivirus Purification	100ml
LV900	qPCR Lentivirus Titration(Titer) Kit	100 Reactions
LV998	Ultra-Pure Lentivirus Purification Kit	1 Kit

更多相關諮詢請洽

https://www.abmgood.com/Lentivirus-Instant-Titer-Card.html

台灣代理廠商: 太鼎生物科技有限公司

公司電話: 02-86609496 公司傳真: 02-86609342

公司網站: www.biopioneer.com.tw

連絡業務: 許虹宜 0920312382

連絡信箱: taiding.biotech@msa.hinet.net

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個人分類：【Lentivirus慢病毒表現、純化】

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rIRS1-HA Adenovirus

胰島素接受體受脢質-1，IRS-1 (Insulin
Receptor Substrate 1) 在”Insulin Receptor與PI 3-kinase之間的訊息傳遞”中扮演關鍵角色。Abmgood提供定量好之IRS1-HA腺病毒液。1x10⁶pfu/mL

IRS1-HA病毒培養液

【IRS1-HA病毒培養液】貨號#286878A。abmgood提供定量好之IRS1-HA adenovirus(NM_012969) 。胰島素接受體受脢質-1病毒培養液，1x10⁶pfu/mL

Cat.No.:	286878A
Quantity:	250ul
Vector	pAdenoG-HA
Vector Size	32908bp
Promoter	CMV
Insert	rIRS1-HA
Insert Size	3708 bp
Organism	Rat
Accession #	NM_012969
Alias	IRS1IRM
Protein Type	insulin receptor substrate 1
Chromosome	9q34
Tags	HA
Gene Type	Wild Type
Titer	1x106pfu/mL
Storage	DMEM with 2.5% glycerol
Shipping	On dry ice.
Caution	This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information.
Notes	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. All sales are final

[供應廠商】太鼎生物科技有限公司

【連絡人】許虹宜 0920-312382 taiding.biotech@msa.hinet.net

【臺北公司】02-86609496

【相關網址】www.biopioneer.com.tw

【原廠網址】www.abmgood.com

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個人分類：【Lentivirus慢病毒表現、純化】

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Dec 01 Sat 2012 17:35
【siRNA Apoptosis Vectors載體】Biovsion提供AIF、Bcl2 Family、Caspase Family、cIAPs、HSP-90 siRNA表現載體

siRNA Apoptosis Vectors載體

siRNA Apoptosis Vectors

BIOVISON提供Apoptosis之siRNA
Apoptosis vectors。包括AIF、Bcl2 Family、Caspase Family、cIAPs、HSP-90
siRNA表現載體

Small
interfering RNAs (siRNAs) are short, double-stranded RNA molecules that can
target and degradate specific complementary mRNAs. The target gene-specific
degradation is an effective means of gene suppression. BioVision's GeneBlocker

pGB siRNA expression vectors are designed to
provide efficient suppression of a target gene in cultured mammalian cells and
in vivo. The pGB vector has been optimized for suppressing expression
of target genes using the human U6 promotor (a RNA polymerase III promotor)
which generates large amounts of siRNA in mammalian cells. The pGB vector
provides neomycin resistance marker for the selection of stable cell lines,
permitting long term suppression of the target gene. BioVision offers siRNA
vectors targeting to important Apoptosis genes. The negative control siRNA
vector and pGB cloning vector for cloning in your own insert are also
available.

AIF

Catalog #	Product Name+	Size
9511-20	pGB AIF siRNA Vector Mix	20 µg
9511-60	pGB AIF siRNA Vector Mix	60 µg

Bcl-2 Family Members

Catalog #	Product Name+	Size
9512-20	pGB BAD siRNA Vector Mix	20 µg
9512-60	pGB BAD siRNA Vector Mix	60 µg
9513-20	pGB BAX siRNA Vector Mix	20 µg
9513-60	pGB BAX siRNA Vector Mix	60 µg
9514-20	pGB Bcl-2 siRNA Vector Mix	20 µg
9514-60	pGB Bcl-2 siRNA Vector Mix	60 µg
9515-20	pGB BID siRNA Vector Mix	20 µg
9515-60	pGB BID siRNA Vector Mix	60 µg

Caspase Family Members

Catalog #	Product Name+	Size
9501-20	pGB Caspase-1 siRNA Vector	20 µg
9501-60	pGB Caspase-1 siRNA Vector	60 µg
9503-20	pGB Caspase-3 siRNA Vector	20 µg
9508-20	pGB Caspase-8 siRNA Vector	20 µg
9509-20	pGB Caspase-9 siRNA Vector	20 µg

cIAPs

Catalog #	Product Name+	Size
9516-20	pGB CIAP-1 siRNA Vector Mix	20 µg
9516-60	pGB CIAP-1 siRNA Vector Mix	60 µg
9517-20	pGB CIAP-2 siRNA Vector Mix	20 µg
9517-60	pGB CIAP-2 siRNA Vector Mix	60 µg

HSP-90

Catalog #	Product Name+	Size
9518-20	pGB Hsp90 siRNA Vector Mix	20 µg
9518-60	pGB Hsp90 siRNA Vector Mix	60 µg

siRNA Cloning Vectors

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個人分類：【Lentivirus慢病毒表現、純化】

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Ultra-Pure Lentivirus Purification
Kit

lentivirus病毒純化及濃度套組，有效提高病毒純度及力價

重組慢病毒lentivirus，廣泛應用於細胞之轉導。轉染後的病毒上清液力價，約106 CFU菌落形成單位（菌落形成單位）/ ml。在高效能病毒力價及體內基因傳遞中，要求較高的病毒純度及力價，且於活體試驗中，避免病毒細胞培養上清液，所造成之污染可能性。因此，病毒上清需要進一步濃縮及純化。

Abmgood提供一套病毒純化套組。

傳統純化病毒，經由超速離心濃縮。雖然該方法效果很好，但需要特定的的超速離心設備，且技術要求高。此外，以濃縮的病毒上清總體積是有限的超速離心管的大小。報告指出，超速離心過程中，會造成一些不利的物理效應，會影響病毒顆粒的生物活性。

Abmgood發展 Ultra-Pure
Lentivirus Purification Kit (Cat. No.
LV998).，針對病毒濃縮技術及純度做改良。提供一個快速且有效，純化及濃縮病毒的產品。其設計原理，為利用離子交換的結合和獨特緩衝液，最大限度減少病毒顆粒的損壞，且提高病毒力價。

Ultra-Pure Lentivirus Purification Kit 套組內容:

(Cat. No. LV998)

• LentiBind Buffer A
(320ml)

• LentiBind Buffer B
(128ml)

• Lenti-Elution buffer
(32ml)

•
LentiBuffer (40ml)

病毒純化步驟

1. On both day 4
and day 5, centrifuge the collected viral supernatant

at 6000g for 10
minutes at 4°C to
pellet the cell debris. Transfer

supernatant to a new
centrifuge container without disturbing the cell

debris.

2. For every
100ml of viral supernatant to be purified, add 20ml of

LentiBind Buffer A and
8.0ml of LentiBind Buffer B. Mix well and shake

the mixture on a
rotating/orbital shaker (commonly used in Western

Blot applications) at
200-300rpm at 4°C
overnight (for day 4 collection).

3. For day 5
viral supernatant collection, allow the binding for 3

hours only and then
combine both collections before proceeding to

step 4.

4. Centrifuge at
7000g at 4°C for 30 mins. to collect the viral particles.

5. Discard
supernatant completely without disturbing the pellet,

and spin briefly again
if necessary to remove any residual liquid.

6. Add 8.0ml
Lenti-Elution buffer to resuspend the viral pellet completely

with a transfer
pipette and transfer the viral suspension to a 10ml

sterile tube that is
resistant to 10,000g during centrifugation in the following step (Sarstedt Cat
No. 62.515.006). Shake the tubes at 4°C for 30min to elute viral particles
from LentiBind Buffer A.

7. After
incubation, spin at 10,000rpm for 20 min to separate viral

particles from the
binding matrix.

8. Transfer the
supernatant (viral particles) to a 10ml syringe and

filter preparation
through a 0.45μM
filter (Millipore, Cat No. SLHV033RB)

to further clarify the
preparation.

9. Perform buffer
exchange for the viral preparation to get rid of

the high salt
concentrations carried over from LentiBind Buffer B using

a 100kDa cut-off
protein concentrating column (Sartorius stedim biotech,

Cat No. VS2042).

10. Spin the
column at 3000rpm at 20°C until the viral supernatant

volume is close to
1-2ml. To speed up the process, use 1ml transfer pipette to re-suspend the
viral preparation every 30 minutes.

11. Add 10ml
LentiBuffer and spin the column again at 3000rpm at

20°C to exchange for final
buffer until the final volume is 200μl-1.0ml

depending on viral
titre desired. To speed up the process, use 1ml

transfer pipette to
re-suspend the supernatant every 30 minutes.

12. Transfer the
final viral preparation from the buffer exchange column

to a 1-3ml syringe and
sterilize it through a 0.45μM filter. Make sure

to pre-wet the filter
with 100μl of
LentiBuffer to prevent virus loss caused

by residual liquid
left in the filter.

13. Final
lentivirus preparations can either be used immediately for

cell transduction or
aliquoted into smaller volumes (10-20μl) for longterm

storage at -75°C.

【供應廠商】太鼎生物科技有限公司

【連絡人】許虹宜 0920-312382 taiding.biotech@msa.hinet.net

【臺北公司】02-86609496 【台中公司】0935-229803

【相關網址】
www.biopioneer.com.tw

【原廠網址】www.abmgood.com

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個人分類：【Lentivirus慢病毒表現、純化】

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qPCR Lentivirus Titration(Titer) Kit

慢病毒qPCR病毒力價----定量套組

Cat.No.:	LV900
Quantity:	100 Reactions

qPCR病毒力價定量---產品介紹

Abmgood提供快速且方便之Lentivirus病毒力價定量套組。其結合一個步驟快速RNA萃取及QPCR技術 (SYBR system)，2個小時完成定量。套組內附有reagent mix, 及primer。primer以HIV based vetors為設計的偵測方式。快速完成病毒力價定量分析。不會有NTC量化問題，完全排除NTC訊號。精確定量Lentivirus titer之方法。

The Lentivirus Titration Kit provides a
fast and simple method for titrating lentivirus. This kit employs a quick RNA extraction
step and determines viral RNA using qRT-PCR. The whole assay could be completed
in only 2 hours. The titer primers supplied in this kit are designed for all
HIV-1 based vectors detection. With the help of the on-line titer calculator,
titration of any lentiviral preparation could be easier than ever before.

qPCR病毒力價定量

優點介紹

Advantages of ABM's Lentiviral qPCR Titer Kit

1. no RNA purification -- saves time and
eliminates inaccuracy

2. ready-to-use reagent mix -- reduces
variability

3. qRT-PCR in one step -- more sensitive
and accurate than other methods

4. no NTC amplification -- our titration
kit is the only one on the market that completely eliminates NTC signal due to
our unique primer design. Similar kits from other companies all give NTC
amplification, which compromise accuracy for viral titration

產品內容

1. Virus Lysis Buffer	800μl
2. Reagent-mix (RT reagent & Primer)	1.0ml
3. STD1 (lentivirus standard 1)	300μl
4. STD2 (lentivirus standard 2)	300μl

標準品STD為lentivirus病毒。

Standard
1濃度為 5x 10^7 IU/ml. Standard 2 濃度為6.25x10^6
IU/ml. 兩者standards 相差 2^3, 相當於Ct 值3次方。

相關商品

LentiVirus病毒濃縮套組

Ultra-Pure Lentivirus Purification Kit

lentivirus病毒純化及濃度套組，有效提高病毒純度及力價

重組慢病毒lentivirus，廣泛應用於細胞之轉導。轉染後的病毒上清液力價，約106 CFU菌落形成單位（菌落形成單位）/ ml。在高效能病毒力價及體內基因傳遞中，要求較高的病毒純度及力價，且於活體試驗中，避免病毒細胞培養上清液，所造成之污染可能性。因此，病毒上清需要進一步濃縮及純化。

Abmgood提供一套病毒純化套組。

傳統純化病毒，經由超速離心濃縮。雖然該方法效果很好，但需要特定的的超速離心設備，且技術要求高。此外，以濃縮的病毒上清總體積是有限的超速離心管的大小。報告指出，超速離心過程中，會造成一些不利的物理效應，會影響病毒顆粒的生物活性。

Abmgood發展 Ultra-Pure Lentivirus Purification Kit (Cat. No. LV998).，針對病毒濃縮技術及純度做改良。提供一個快速且有效，純化及濃縮病毒的產品。其設計原理，為利用離子交換的結合和獨特緩衝液，最大限度減少病毒顆粒的損壞，且提高病毒力價。

Ultra-Pure Lentivirus
Purification Kit

套組內容

(Cat. No. LV998)

• LentiBind Buffer A (320ml)

• LentiBind Buffer B (128ml)

• Lenti-Elution buffer (32ml)

•LentiBuffer (40ml)

【供應廠商】太鼎生物科技有限公司

【連絡人】許虹宜 0920-312382 taiding.biotech@msa.hinet.net

【臺北公司】02-86609496 【台中公司】0935-229803

【相關網址】 www.biopioneer.com.tw

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個人分類：【Lentivirus慢病毒表現、純化】

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Aug 30 Thu 2012 00:10
【3'UTR miRNA Reporter 】luciferase/GFP二種系統。另提供建立好之miRNA Reporter細胞株。Abmgood

3'UTR miRNA Reporter (luciferase/GFP二種系統)

另提供建立好之miRNA Reporter細胞株

One
of the most reliable, quantitative assays for the suppression of target genes
by a specific miRNA is the utilization of a reporter gene like luciferase or
GFP. Target site for a specific miRNA is subcloned downstream of the reporter
gene and co-transfected along with a miRNA expressing vector into cells.
Inhibition of reporter genes by the expressed miRNA as compared with valid
controls proves the regulation of genes with target site at 3'-UTR by the
specific miRNA. To facilitate your research on genes of your interests, we have
developed 3'-UTR library of lentiviral vectors for all human genes down-stream
a reporter gene of either luciferase or GFP. The advantage of lentiviral vector
is its flexibility for either transfection or viral vector infection

Luciferase

GFP

Stable Cells

3'UTR Vectors

Gene Name	Acce. No.	Vector	Size	Cat.No.
Blank (Control) Vector	N/A	pLenti-UTR-Luc-Blank	1ug	m012
A1BG 3'UTR Lenti-reporter-Luc Vector	NM_130786	pLenti-UTR-Luc	1ug	MT-h00001

3'UTR Virus

Gene Name	Acce. No.	Vector	Viral Titer	Size	Cat.No.
Blank (Control) Virus	N/A	pLenti-UTR-Luc-Blank	10⁶inf/ml	3.0ml	m013
A1BG 3'UTR Lenti-reporter-Luc Virus	NM_130786	pLenti-UTR-Luc	10⁶inf/ml	3.0ml	MV-h00001

3'UTR Vectors

Gene Name	Acce. No.	Vector	Size	Cat.No.
Blank (Control) Vector	N/A	pLenti-UTR-GFP-Blank	1ug	m014
A1BG 3'UTR Lenti-reporter-GFP Vector	NM_130786	pLenti-UTR-GFP	1ug	MT-h50001

3'UTR Virus

Gene Name	Acce. No.	Vector	Viral Titer	Size	Cat.No.
Blank (Control) Virus	N/A	pLenti-UTR-GFP-Blank	10⁶inf/ml	3.0ml	m015
A1BG 3'UTR Lenti-reporter-GFP Virus	NM_130786	pLenti-UTR-GFP	10⁶ifu/ml	3.0ml	MV-h50001

3'UTR
Luciferase Stable Cell Lines

Product Name	Acc. No.	Cell Line	Concentration	Size	Cat.No.
A1BG 3'UTR Luciferase Stable Cell Line	NM_130786	293 Cells	10^6 cells/ml	1.0ml	TU000001
Blank (Control) 3'UTR Luciferase Stable Cell Line	N/A	293 Cells	10⁶ cells/ml	1.0ml	m018

3'UTR GFP
Stable Cell Lines

Product Name	Acc. No.	Cell Line	Concentration	Size	Cat.No.
A1BG 3'UTR GFP Stable Cell Line	NM_130786	293 Cells	10^6 cells/ml	1.0ml	TU050001
Blank (Control) 3'UTR GFP Stable Cell Line	N/A	293 Cells	10⁶ cells/ml	1.0ml	m019

【供應廠商】太鼎生物科技有限公司

【連絡人】許虹宜 0920-312382

【臺北公司】02-86609496

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個人分類：【Lentivirus慢病毒表現、純化】

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Jul 04 Wed 2012 23:49
【Packaging System】Lentivirus病毒顆粒表現系統。2nd Generation 。貨號#LV003,

2nd Generation
Packaging System

Product	Vector Map (Click to View)	Select. Marker	Quantity	Cat. #
2^nd Generation Packaging System Mix	pLenti-P2A pLenti-P2B	N/A	200ul	LV003
GFP Control Lentivirus	pLenti-GFP	Neomycin	10ml	LV006
β-Gal Control Lentivirus	Map Unavailable	Neomycin	10ml	LV007

pLenti-P 2A

pLenti-P2B

Description

For the production of lentiviral particles, three components are
generally required: 1) a lentiviral vector containing your inserts of interests
(cDNA, shRNA, or miRNA), 2) one or two packaging vectors which contain all
necessary viral structure proteins, 3) an envelope vector expressing Vesicular
Stomatitis Virus (VSV) glycoprotein (G). In general, lentiviral vectors with a
wildtype 5' LTR need the 2^nd(second) generation packaging system because these vectors require TAT
for activation. However, the 2^nd(second) generation packaging mix will also support the production of 3^rd(third) lentivral expression vector with a
chimeric 5' LTR. All the lentiviral expression vectors market by ABM are 3^rd(third) generation with a chmeric of RSV
promoter upstream of 5'-LTR.

【供應廠商】太鼎生物科技有限公司

【連絡人】許虹宜0920-312382

【台北公司】02-86609496

【相關網址】www.biopioneer.com.tw

【原廠網址】http://www.abmgood.com

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個人分類：【Lentivirus慢病毒表現、純化】

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Jul 04 Wed 2012 01:24
【GFP hsa-miR-21 miRNA/microRNA Lentivector】貨號#mh10276, mir21 GFP microRNA 表現質體。

Abmgood提供miRNA
GFP表現載體、miRNA 3’ UTR
reporter-luciferase vector、miRNA inhibitor等產品。

GFP hsa-miR-21 miRNA/microRNA Lentivector

Cat.No.:	mh10276
Quantity:	500ng

miRNA ID	MI0000077
miRNA Name	hsa-mir-21
Accession No.	MI0000077
Genome Location	Chromosome 17:55273409:55273480 (+ Strand )
Species	Human
Insert Type	Pre-miRNA
Reporter	GFP
Vector Name	pLenti-III-mir-GFP
miRNA ID	MI0000077

pLenti-III-mir-GFP-Blank

Cat.No.:	m001
Quantity:	500ng
miRNA Name	Blank
Reporter	GFP
Vector Name	pLenti-III-mir-GFP-Blank

miRNA Lentivirus
相關產品

Kit Name	Size	Cat.No.
Complete Kit for 2nd Generation miRNA	1 Kit	MK001
Complete Kit for 3rd Generation miRNA	1 Kit	MK002
Partial Kit for 2nd Generation miRNA	1 Kit	MK003
Partial Kit for 3rd Generation miRNA	1 Kit	MK004
Ultra-Pure Lentivirus Purification Kit	1 Kit	LV998

【供應廠商】太鼎生物科技有限公司

【連絡人】許虹宜
0920-312382

【台北公司】02-86609496

【相關網址】
www.biopioneer.com.tw

【原廠網址】http://www.abmgood.com

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個人分類：【Lentivirus慢病毒表現、純化】

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Jul 04 Wed 2012 01:15
【GFP hsa-miR-16 miRNA/microRNA Lentivector】貨號mh11053, mir-16 GFP mircroRNA表現質體。

Abmgood提供miRNA
GFP表現載體、miRNA 3’ UTR
reporter-luciferase vector、miRNA inhibitor等產品。

GFP hsa-miR-16 miRNA/microRNA Lentivector

Cat.No.:	mh11053
Quantity:	500ng

miRNA ID	MIMAT0000069
miRNA Name	hsa-miR-16
Accession No.	MIMAT0000069
Species	Human
Insert Type	Mature miRNA
Reporter	GFP
Vector Name	pLenti-III-mico-GFP
miRNA ID	MIMAT0000069
miRNA Name	hsa-miR-16

pLenti-III-mir-GFP-Blank

Cat.No.:	m001
Quantity:	500ng
miRNA Name	Blank
Reporter	GFP
Vector Name	pLenti-III-mir-GFP-Blank

miRNA Lentivirus
相關產品

Kit Name	Size	Cat.No.
Complete Kit for 2nd Generation miRNA	1 Kit	MK001
Complete Kit for 3rd Generation miRNA	1 Kit	MK002
Partial Kit for 2nd Generation miRNA	1 Kit	MK003
Partial Kit for 3rd Generation miRNA	1 Kit	MK004
Ultra-Pure Lentivirus Purification Kit	1 Kit	LV998

【供應廠商】太鼎生物科技有限公司

【連絡人】許虹宜
0920-312382

【台北公司】02-86609496

【相關網址】
www.biopioneer.com.tw

【原廠網址】http://www.abmgood.com

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個人分類：【Lentivirus慢病毒表現、純化】

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Jul 04 Wed 2012 00:52
【PTEN 3'UTR Lenti-reporter luciferase vector產品資料】貨號#MT-h19140, PTEN 3'UTR reporter。

Abmgood提供miRNA
3’ UTR reporter-luciferase vector、miRNA inhibitor、miRNA表現載體等產品。

PTEN 3'UTR
Lenti-reporter-Luciferase Vector

Cat.No.:	MT-h19140
Quantity:	1ug

Full Name	phosphotriesterase related
Accession No.	NM_030664
Organism	Human
Chromosome Loc.	10p12
Vector	pLenti-UTR-Luc
Titer	N/A
Storage	-20°C for up to a year.
User Manual
Background Reading

HMGA1 3'UTR
Lenti-reporter-Luciferase Vector-Blank

Cat.No.:	m012
Quantity:	1ug

【供應廠商】太鼎生物科技有限公司

【連絡人】許虹宜
0920-312382

【台北公司】02-86609496

【相關網址】
www.biopioneer.com.tw

【原廠網址】http://www.abmgood.com

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個人分類：【Lentivirus慢病毒表現、純化】

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Jul 04 Wed 2012 00:42
【HMGA1 miRNA 3'UTR reporter-luciferase vector】貨號MT-h09952, mir-16 3'UTR reporter。

Abmgood提供miRNA
3’ UTR reporter-luciferase vector、miRNA inhibitor、miRNA表現載體等產品。

HMGA1 3'UTR
Lenti-reporter-Luciferase Vector

Cat.No.:	MT-h09952
Quantity:	1ug

Full Name	high mobility group AT-hook 1
Accession No.	NM_145899
Organism	Human
Chromosome Loc.	6p21
Vector	pLenti-UTR-Luc
Titer	N/A
Storage	-20°C for up to a year.
User Manual
Background Reading

HMGA1 3'UTR
Lenti-reporter-Luciferase Vector-Blank

Cat.No.:	m012
Quantity:	1ug

【供應廠商】太鼎生物科技有限公司

【連絡人】許虹宜
0920-312382

【台北公司】02-86609496

【相關網址】
www.biopioneer.com.tw

【原廠網址】http://www.abmgood.com

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Jul 04 Wed 2012 00:22
【hsa-let-7g mircoRNA Primer】貨號MPH01012, hsa-let-7g microRNA primer設計與合成。

Abmgood提供miRNA
QPCR偵測套組，一系列產品包括有miRNA primer、universal
primer、Evagreen Mastermix等產品。

miRNA Primer Results for: hsa-let -7g
primer

hsa-let-7g Primers	Human	MIMAT0000414	150ul/10uM	MPH01012
Universal 3' miRNA Primer	Universal	N/A	150ul/10uM	MPH00000

Cat.No.:	MPH01012
Size:	150ul/10uM
Gene Name	hsa-let -7g
miRNA ID	MIMAT0000414
miRNA Name	hsa-let -7g
Accession No.	MIMAT0000414
Species	Human
Storage conditions	-20°C
Background Reading

【U6-2 miRNA Primers對照組】miRNA(microRNA)
primer

U6-2 Primers	150ul/10uM	MPH00001
U6 snRNA Primers	150ul/10uM	MPM00002

【供應廠商】太鼎生物科技有限公司

【連絡人】許虹宜
0920-312382

【台北公司】02-86609496

【相關網址】
www.biopioneer.com.tw

【原廠網址】http://www.abmgood.com

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Jul 04 Wed 2012 00:12
【hsa-let -7a microRNA primer產品資料】貨號#MPH01006, hsa-let-7a primer設計與合成

Abmgood提供miRNA
QPCR偵測套組，一系列產品包括有miRNA primer、universal
primer、Evagreen Mastermix等產品。

miRNA Primer Results for: hsa-let -7a
primer

Product Name	Species	Access. No.	Size	Cat. No.
hsa-let-7a Primers	Human	MIMAT0000062	150ul/10uM	MPH01006
Universal 3' miRNA Primer	Universal	N/A	150ul/10uM	MPH00000

Cat.No.:	MPH01006
Size:	150ul/10uM
Gene Name	hsa-let -7a
miRNA ID	MIMAT0000062
miRNA Name	hsa-let -7a
Accession No.	MIMAT0000062
Species	Human
Storage conditions	-20°C
Background Reading

【U6-2 miRNA Primers對照組】miRNA(microRNA) primer 貨號#MPH0001。QPCR定量對照組。

http://tw.myblog.yahoo.com/taiding2000/article?mid=534&prev=535&next=517&l=f&fid=12

U6-2 Primers	150ul/10uM	MPH00001
U6 snRNA Primers	150ul/10uM	MPM00002

【供應廠商】太鼎生物科技有限公司

【連絡人】許虹宜
0920-312382

【台北公司】02-86609496

【相關網址】
www.biopioneer.com.tw

【原廠網址】http://www.abmgood.com

太鼎生物科技發表在痞客邦留言(0) 人氣()

個人分類：【Lentivirus慢病毒表現、純化】

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Jul 03 Tue 2012 16:09
【U6-2 miRNA Primers對照組】miRNA(microRNA) primer 貨號#MPH0001。QPCR定量對照組。

Abmgood提供miRNA QPCR偵測套組，一系列產品包括有miRNA primer、universal primer、Evagreen Mastermix等產品。

miRNA Primers

All our primers have been validated for accuracy,
extensively studied to obtain their optimal qPCR reaction conditions and
optimized to work with our qPCR miRNA Mastermix. Also, they cover every miRNA
characterized in the Sanger 16.0 database release (September 2010).

U6-2 Primers

Cat.No.:	MPH00001
Size:	150ul/10uM

Gene Name	U6-2
miRNA ID	NR_002752.1
miRNA Name	U6-2
Accession No.	NR_002752.1
Species	Human

【供應廠商】太鼎生物科技有限公司

【連絡人】許虹宜 0920-312382

【台北公司】02-86609496

【相關網址】
www.biopioneer.com.tw

【原廠網址】http://www.abmgood.com

更多microRNA問與答資料整理:

http://tw.myblog.yahoo.com/taiding2000/article?mid=535&next=534&l=f&fid=12

太鼎生物科技發表在痞客邦留言(0) 人氣()

個人分類：【Lentivirus慢病毒表現、純化】

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Jul 03 Tue 2012 14:29
【CD20 (MS4A1)抗體、抗原、siRNA、3'UTR reporter】abmgood CD20產品系列。

CD20 (MS4A1)抗體、抗原、siRNA、3'UTR reporter

【供應廠商】太鼎生物科技有限公司

【連絡人】許虹宜 0920-312382

MS 4A 1抗體

CD20
/ MS4A1 (C Terminus)

200ul

100µg/200ul

Y212762

MS 4A 1
siRNA lentivirus

Product	Accession Number	Size	Cat. No.
MS4A1-set	NM_021950	4x500ng	i014282
MS4A1	NM_021950	500ng	i 014282a
MS4A1	NM_021950	500ng	i014282b
MS4A1	NM_021950	500ng	i 014282c
MS4A1	NM_021950	500ng	i014282d

MS 4A 1 lentivirus

pLenti-CMV-m-Ms4a1	NM_007641.5	1ug	LV031490
pLenti-CMV-rat-Ms4a1	NM_001107578.1	1ug	LV054901

MS 4A 1
Lysate

hMS4A1 Positive Lysate	100ug	PL035497
hMS4A1 Positive Lysate with C-Ha Tag	100ug	PL035498
rMs4a1 Positive Lysate	100ug	PL138585

MS 4A 1
蛋白質

hMS4A1 Recombinant Protein	5ug	RP070993
hMS4A1-C HA Tag Recombinant Protein	5ug	RP070994
hMS4A1-N His Tag Recombinant Protein	5ug	RP070995
hMS4A1-C His Tag Recombinant Protein	5ug	RP070996
rMs4a1 Recombinant Protein	5ug	RP277169
rMs4a1-C HA Tag Recombinant Protein	5ug	RP277170
rMs4a1-N His Tag Recombinant Protein	5ug	RP277171
rMs4a1-C His Tag Recombinant Protein	5ug	RP277172