太鼎生物科技/太鼎食安科技

PAP 免疫染色用筆 Super PAP Pen | Liquid Blocker PAP Pen Servicebio貨號G6100

產品介紹:

阻水筆 / 免疫阻化筆/免疫染色筆
使用前請檢查筆尖，上下搖晃筆使其均勻，按下筆尖時使液體向下流。
可在載玻片上形成疏水外圈, 廣泛用於免疫染色、和螢光抗體法、原位雜交、特殊染色。正確使用， 次數可達500次以上

PAP 免疫染色用筆 Super PAP Pen

Liquid Blocker PAP Pen是一種特殊的記號筆(marker pen)，在使用免疫組化筆在載玻片上的組織周圍畫圈時，可提供薄膜狀(thin film-like)疏水屏障(hydrophobic barrier)。在不影響實驗結果的情況下，可有效減少試劑用量，避免表面試劑流失造成的乾片和脫包(decapsulation)。適用於免疫組織染色(immunohistochemistry)、免疫螢光(immunohistochemistry)、原位雜交(immunohistochemistry)、特殊染色(immunohistochemistry)等。該產品採用優化的化學配方，可承受乙醇脫水(ethanol dehydration)和水化步驟(hydration steps)，以及原位雜交(in situ hybridization)變性(denaturation)所需的高溫操作，測試時的耐熱性低於120℃。本產品用於載玻片anti-dropping treatment。內含5毫升筆液，正確使用可畫圓約500次。

Servicebio台灣品牌介紹-BRAND/AGENCY/品牌介紹- Servicebio

Motic EASYSCAN 全景玻片掃描器

Motic EASYSCAN提供高解析度、高通量、低門檻的玻片掃瞄系統

Motic EASYSCAN 儀器特點

• 1. 使用高NA物鏡，提供最佳解析度 (0.25μm/pixel)
• 2. 6片式片夾，walk-away設計
• 3. 提供2種相機選擇
• 4. 高速、高精準自動對焦
• 5. 3D多層次掃描
• 6. 多種掃描模式，滿足每一個樣品需求
• 7. 提供完整的切片分享解決方案
• 8. 檔案格式相容於多種虛擬玻片觀看軟體

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6片式片夾，Walk-away自動化設計

高NA物鏡，提供高解析度掃描結果

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台灣代理廠商: 太鼎生物科技有限公司
公司電話: 02-86609496 /0920312382
公司傳真: 02-86609342
公司網站: www.biopioneer.com.tw
連絡業務: 許虹宜 0920312382
連絡信箱: taiding.biotech@msa.hinet.net

NEGATIVE CONTROL SERA

Negative control sera and isotype controls are employed in place of primary antibody for staining negative control slides. Negative control slides void of staining verifies the staining specificity of the primary antibody for the target antigen as stained in positive control and case slides.

Innovex Biosciences offers a wide selection of negative control sera and isotype controls for different sources of primary antibodies employed.

Negative Control Sera

Please click this icon to download the PDF file.

Item#	Product	Size	Price
NCP801R	Rabbit	7 ml	$95
NCM802M	Mouse (ascites fluid)	7 ml	$95
NC909	Sheep	7 ml	$95
NC912	Goat	7 ml	$89
NC914	Chicken	7 ml	$175
NC915	Rat	7 ml	$165

SIGNAL ENHANCING WASH BUFFERS

A Room temperature retrieval
and stain amplification without pre-treatments

ADVANCED FEATURES
• Simple to use, employ as the rinse buffer in place of PBS for rinsing in between incubation steps
• Amplify staining signal by 2-4 folds with no additional steps added to the immunostaining procedure
• Cost effective: Allows 2-4 folds increase for primary antibody dilution
• Time Saver : Allows for decrease in primary antibody incubation time
• Allows for decrease in chromogen/substrate incubation time
• Fixation independent; Amplifies staining regardless of the type of fixative employed
• Eliminates enzyme pre-digestion
• Minimizes the need for high heat antigen recovery when used with INNOVEX Staining Systems
• Minimizes false negative staining
• Automation compatible
• Applicable to ELISA and flow cytometric procedures

USER FRIENDLY PROTOCOL
Innovex Enhancing Wash Buffers only require 5 seconds rinse in between incubation steps when used with Innovex STAT-Q and HISTO-STAT ( 2-step) staining systems. Innovex Enhancing Wash Buffers may be employed with other systems, observe the manufacturer’s instruction for the required time and number of washes recommended for the system.

1. Rinse specimens with Innovex Enhancing Wash Buffer prior to applying primary antibody;
2. Incubate with primary antibody per manufacturer’s instructions;
3. Rinse with Innovex Enhancing Wash Buffer;
4. Incubate with secondary antibody;
5. Rinse with Innovex Enhancing Wash Buffer;
6. Incubate with Enzyme label;
7. Rinse with Innovex Enhancing Wash Buffer;
8. Incubate with substrate/chromogen of choice;
9. Rinse with water;
10. Counterstain and mount.

For ELISA assays
Innovex Enhancing Wash Buffers can be used for washing ELISA plates, however, some non-ionic detergent should be added to the solution.

SIGNAL ENHANCER WASH BUFFERS ARE AVAILABLE IN:

Enhancing Wash Buffer for retrieving
AEC and DAB stains

H&E Mount

HE染色/封片劑
No alcohol or xylene clearing pre-steps required

         太鼎生物科技有限公司 (02) 8660-9496 www.biopioneer.com.tw 

FOR PERMANENT MOUNTING OF H&E STAINS FROM WATER OR ALCOHOL

“H&E Mount” is a unique, permanent mounting media designed for coverslipping H&E stains from water or alcohol.
“H&E Mount” hardens quickly and mounted slides are ready for microscopic examination in 10 minutes.
H&E stained slides mounted with “H&E Mount” do not display any loss of eosin or hematoxylin staining that is often observed when H&E slides are mounted from xylene. Therefore, when mounting with “H&E Mount”, less number of dips
(5-10) in eosin is recommended.

www.innovexbiosciences.com

Fc Receptor Blocker

ADVANCED and UNIQUE FEATURES:
• 30 minute incubation step at room temperature
• Blocks Fc Receptors in both human and animal cells
• Eliminates false positive staining of white blood cells, lymphoid tissues, cytosmears (blood & bone marrow)
• Insures accurate lymphoma and leukemia typing
• Eliminates negative control staining of lymphomas, lymphoid tissues, and blood and bone marrow smears
• Eliminates false positive staining for Kappa and Lambda staining
• Eliminates false positive staining of Reed Sternberg cells
• A must for CD markers staining in immunohistochemistry, immunofluorescence
• A must for CD phenotyping via flow cytometry
• A must for Immunoglobulins (Igs) staining via immunohistochemistry, flow cytometry and immunofluorescence
• A must for kappa and lambda staining

Innovex Fc Receptor Blocker does not contain any antibodies, immunoglobulins or immunoglobulin fragments 

Now also available in azide-free for live cell/functional assays
Azide-Free Fc Blocker can be used on live cells and for functional assays without cytotoxicity
Ordering info, scroll down

Innovex Fc Receptor Block is a peerless technology designed to block Fc Receptors present on all leukocytes (white blood cells), lymphomas, and melanomas. Fc receptors are also expressed on a majority of tumors. Blocking Fc receptors is essential for accurate typing of lymphoid and tumor tissues and cells. Fc receptor staining occurs by the binding of Fc receptors present on cell to the Fc region of the primary and/or secondary antibody. Fc Receptor Block can be used for IHC (frozen and paraffin) flow cytometry, immunoflorescence and in-situ hybridization and for live cell functional assays.

Fc Receptor Blocker is a must for accurate lymphoma and leukemia typing.

ADVANCED and UNIQUE FEATURES:
• 30 minute incubation step at room temperature
• Blocks Fc Receptors in both human and animal cells
• Eliminates false positive staining of white blood cells, lymphoid tissues, cytosmears (blood & bone marrow)
• Insures accurate lymphoma and leukemia typing
• Eliminates negative control staining of lymphomas, lymphoid tissues, and blood and bone marrow smears
• Eliminates false positive staining for Kappa and Lambda staining
• Eliminates false positive staining of Reed Sternberg cells
• A must for CD markers staining in immunohistochemistry, immunofluorescence
• A must for CD phenotyping via flow cytometry
• A must for Immunoglobulins (Igs) staining via immunohistochemistry, flow cytometry and immunofluorescence
• A must for kappa and lambda staining

 

Please click this icon to download the data sheet in PDF file.

 

Item#	Size	# of Slides	Shelf life		Buy
NB309	60 ml	700-1000	3 yrs
NB309-15	15 ml	150 - 250	3 yrs
NB335 (NEW)	30 ml	Azide - Free	1 yr

 

 

【如何移除免疫組織染色的背景值】

在做mouse組織切片時，很容易發生backgroud的問題。

尤其是冷凍切片的組織。Innovex Fc Receptor Block跟您的背景值說再見!!

特別應用於IHC、IF、In situ hybridation實驗。

【Innovex Fc Receptor Block】 引先最新科技設計，針對白血球 leukocytes (white blood cells),淋色瘤 lymphomas, 及黑色素瘤 melanomas部份進行專一性阻斷。

組織內大量存在Fc receptors；Fc receptors是一種glycoproteins, 存在於血液中B細胞、單核細胞、顆粒細胞 dendritic細胞。組織內Fc receptors與抗體中Fc region具高親和力，FcRs-mediated Ig Fc binding 造成非專一性及背景值形成。Fc receptor也是大量存在大部份的腫瘤細胞組織中，因此在免疫組織染色IHC (frozen and paraffin)、流氏細胞分析 flow cytometry, 免疫螢光染色 immunoflorescence 及原位雜合反應試 in-situ hybridization，會使用Fc receptor Blocker 除去抗體Fc region所有非專一性及背景值的問題，尤其是處理血液、骨髓或淋巴的組織!!!

 Fc receptor Blocker 先在組織切片中作用15~30分鐘，之後加入一抗或二抗，就不會造成抗體中的Fc region與組織Fc receptor反應。

Fc receptors are also expressed on a majority of tumors. Blocking Fc receptors is essential for accurate typing of lymphoid and tumor tissues and cells. Fc receptor staining occurs by the binding of Fc receptors present on cell to the Fc region of the primary and/or secondary antibody. Fc Receptor Block can be used for IHC (frozen and paraffin) flow cytometry, immunoflorescence and in-situ hybridization and for live cell functional assays.

IHC過程中，若是使用biotin及avidin的系統， 建議使用biotin/avidin的blocking buffer，在抗體反應之前，先移去內源性biotin/avidin。如此就不會有非一性binding的問題。

【產品特點與規格】

• 30 minute incubation step at room temperature
• Blocks Fc Receptors in both human and animal cells
• Eliminates false positive staining of white blood cells, lymphoid tissues, cytosmears (blood & bone marrow)
• Insures accurate lymphoma and leukemia typing
• Eliminates negative control staining of lymphomas, lymphoid tissues, and blood and bone marrow smears
• Eliminates false positive staining for Kappa and Lambda staining
• Eliminates false positive staining of Reed Sternberg cells
• A must for CD markers staining in immunohistochemistry, immunofluorescence
• A must for CD phenotyping via flow cytometry
• A must for Immunoglobulins (Igs) staining via immunohistochemistry, flow cytometry and immunofluorescence
• A must for kappa and lambda staining

【如何降低免疫組織染色過程中背景值 第一部份】

IHC過程中，若是使用biotin及avidin的系統， 建議使用biotin/avidin的blocking buffer，在抗體反應之前，先移去內源性biotin/avidin。如此就不會有非一性binding的問題。原理:阻斷內源性biotin (rich in liver,kidney,spleen) 所導致背景值的問題 適用於任何human和animal組織 。

 適用於任何human和animal組織

去除不必要的背景，呈現組織真正面貌

Fc RECEPTOR BLOCK
Peptide Based Technology

N O _M O R E _Fc Receptor Staining
N O _M O R E  background

Innovex Fc Receptor Blocker does not contain any antibodies, immunoglobulins or immunoglobulin fragments 

Now also available in azide-free for live cell/functional assays
Fc Blocker can be used on live cells and for functional assays without cytotoxicity
Ordering info, scroll down

Innovex Fc Receptor Block is a peerless technology designed to block Fc Receptors present on all leukocytes (white blood cells), lymphomas, and melanomas. Fc receptors are also expressed on a majority of tumors. Blocking Fc receptors is essential for accurate typing of lymphoid and tumor tissues and cells. Fc receptor staining occurs by the binding of Fc receptors present on cell to the Fc region of the primary and/or secondary antibody. Fc Receptor Block can be used for IHC (frozen and paraffin) flow cytometry, immunoflorescence and in-situ hybridization and for live cell functional assays.

Fc Receptor Blocker is a must for accurate lymphoma and leukemia typing.

ADVANCED and UNIQUE FEATURES:
• 30 minute incubation step at room temperature
• Blocks Fc Receptors in both human and animal cells
• Eliminates false positive staining of white blood cells, lymphoid tissues, cytosmears (blood & bone marrow)
• Insures accurate lymphoma and leukemia typing
• Eliminates negative control staining of lymphomas, lymphoid tissues, and blood and bone marrow smears
• Eliminates false positive staining for Kappa and Lambda staining
• Eliminates false positive staining of Reed Sternberg cells
• A must for CD markers staining in immunohistochemistry, immunofluorescence
• A must for CD phenotyping via flow cytometry
• A must for Immunoglobulins (Igs) staining via immunohistochemistry, flow cytometry and immunofluorescence
• A must for kappa and lambda staining

                                                          BACKGROUND BUSTER

N O _M O R E _B A C K G R O U N D

Please click this icon to download the data sheet in PDF file.

• Mouse-On-Mouse
  
• Human and Animal IHC
  
• Immunofluorescence
  
• in-situ Hybridization
  
• Flow Cytometry

Background Buster is highly effective for quenching background fluorescence.

Easy to use:

• Apply Background Buster for 15 minutes prior to application of the first antibody (unlabeled or fluorescence-labeled antibody).
  
• Rinse with PBS for 1 minute.
  
• Apply unlabeled antibody for indirect method or fluorescence labeled antibody for direct method and continue with usual protocol.

Please click this icon to download the data sheet in PDF file.

Background Buster can be used for IHC:

Background or non-specific staining is often observed in a variety of immunoassays, in immunohistochemistry and other immunoassay types such as immunofluorescence, ELISA and flow cytometric assays, background staining can be prevented by the use of INNOVEX Recombinant Protein Technology, Background Buster.

Innovex Background Buster is a diverse and powerful RECOMBINANT PROTEIN TECHNOLOGY for eliminating general background staining in tissues and cell preparations stained with antibodies in immunohistochemistry or with DNA or RNA probes for in-situ procedures. It also makes possible the staining of mouse antibodies on mouse tissues.

Background Buster is also applicable to eliminating non-specific binding in immunofluorescence, ELISA and flow cytometric assays.

ADVANCED & UNIQUE FEATURES:

• Allows staining of identical species antibodies and tissues (e.g., mouse antibody on mouse tissue, rat-on-rat, rabbit-on-rabbit, etc.).
• Short 10 minute incubation step prior to applying primary antibody or in-situ probe at room temperature
• Delivers complete eradication of general background staining
• Replaces the use of normal serum, powdered milk, casein, and other blocking agents and renders complete success
• Excellent for both frozen and paraffin sections
• Excellent for in situ application
• A must for animal tissue staining

更多參考內容:

WWW.BIOPIONEER.COM.TW

太鼎生物科技有限公司(02)86609496

Total Proteins

分離自約200個不同成人與胎兒的正常組織、人類疾病或腫瘤組織，以及小鼠、大鼠、猴子與植物組織。研究不同組織的蛋白質表現時，總蛋白質是應用最廣泛的產品。

產品特色

組織總蛋白質由完整組織經均質化而得，經 SDS-PAGE 驗證具有相同的成分。

總蛋白質保存於具有綜合蛋白脢抑制劑的緩衝溶液中，其濃度為 5 m g/ml。

保存於 -70 oC 具有0.1mg/ 0.5m g/ 1m g包裝

應用範圍

電泳、西方墨點轉印、免疫沉澱
酵素活性分析

蛋白質分子間作用力分析

特定組織之基因表現研究

Total Protein Arrays

取自具文件紀錄之組織或細胞株，組織經切除後立即冷凍，進行病理鑑定。
利用 CytoMol 專屬技術分離出總蛋白質，並點於特殊硝化纖維薄膜上。
以 GAPDH 抗體進行免疫分析以確保品質與蛋白量一致。
本產品可立即使用，不需耗費時間與精力於自行尋找組織與取得所需蛋白樣本

產品特色

可使用微陣列掃瞄器或一般自動放射顯像法偵測

蛋白質預點，可立即使用

可配合組織陣列與 mRNA 陣列使用

適於放射性與非放射性偵測方式

可選擇隨附組織臨床病史

可選購相同組織之切片或 cDNA 以進行免疫化學、原位雜合或 PCR 偵測

單一陣列內涵括所有類型之成人與胎兒正常組織蛋白，以及人類腫瘤組織蛋白

應用範圍

微陣列掃描器的大量篩選或一般照片顯影

針對大範圍之正常成人、胎兒或腫瘤組織進行特定蛋白質之快速篩選

蛋白質表現 pattern 分析

目標蛋白質之表現數量比較

Proteins form human tissues
Adult Tissue
Adult and Fetal Tissues
Fetal Tissue
Tumor Tissue

Tissue Arrays

Tissue Arrays 組織陣列

將組織點於帶正電之玻片上
每片 slide 包含15種不同動物的正常組織

用以測試抗體的物種專一性
PAR34035 Multiple Species Frozen Tissue Array – Brain, 2 slides
PAR34036 Multiple Species Frozen Tissue Array – Brain, 5 slides
PAR34149 Multiple Species Frozen Tissue Array – Liver, 2 slides
PAR34150 Multiple Species Frozen Tissue Array – Liver, 5 slides

Total Protein Western Blots

產品特色
組織蛋白經過4-20% gradient SDS-PAGE 後轉印至PVDF membrane
每個 lane loading 一個 tissue 的 total proteins
內含人類胎盤蛋白作為internal standard 以判別蛋白表現量
帶有10KD-250KD的 prestained protein marker
本產品可立即使用，不需耗費時間與精力
於自行尋找組織與取得所需蛋白樣本

應用範圍

Identifying specific protein expression 

Determining protein size

Analyzing protein expression pattern

Comparing expression levels of novel proteins

Examining alternative splicing and premature termi-
nation of a specific protein

Proteins From Human Tissues
Adult Tissue
Fetal Tissue
Tumor Tissue (DD)
Tumor Tissue (SD)
Tumor Cell Lines

Proteins From Non-Human Tissues
Monkey (Cynomolgus)
Monkey (Rhesus)
Mouse
Plant
Rat
Other species

Mega Western Protein Arrays

total proteins 經 SDS-PAGE 分離後，每個 lane 分為 24 fractions, 點於特殊硝基纖維薄膜
以 GAPDH 抗體進行免疫分析以確保品質與蛋白質量一致

產品特色

品質穩定，結果具再現性
靈敏度更高，可用於放射性與非放射性檢測
操作容易，與一般 western blotting 相同
單一晶片可檢測30個不同組織
晶片大小為 2×1 inch2，節省抗體用量 (為傳統 western blot 的 ¼)