太鼎生物科技/太鼎食安科技

【如何移除免疫組織染色的背景值】

在做mouse組織切片時，很容易發生backgroud的問題。

尤其是冷凍切片的組織。Innovex Fc Receptor Block跟您的背景值說再見!!

特別應用於IHC、IF、In situ hybridation實驗。

【Innovex Fc Receptor Block】 引先最新科技設計，針對白血球 leukocytes (white blood cells),淋色瘤 lymphomas, 及黑色素瘤 melanomas部份進行專一性阻斷。

組織內大量存在Fc receptors；Fc receptors是一種glycoproteins, 存在於血液中B細胞、單核細胞、顆粒細胞 dendritic細胞。組織內Fc receptors與抗體中Fc region具高親和力，FcRs-mediated Ig Fc binding 造成非專一性及背景值形成。Fc receptor也是大量存在大部份的腫瘤細胞組織中，因此在免疫組織染色IHC (frozen and paraffin)、流氏細胞分析 flow cytometry, 免疫螢光染色 immunoflorescence 及原位雜合反應試 in-situ hybridization，會使用Fc receptor Blocker 除去抗體Fc region所有非專一性及背景值的問題，尤其是處理血液、骨髓或淋巴的組織!!!

 Fc receptor Blocker 先在組織切片中作用15~30分鐘，之後加入一抗或二抗，就不會造成抗體中的Fc region與組織Fc receptor反應。

Fc receptors are also expressed on a majority of tumors. Blocking Fc receptors is essential for accurate typing of lymphoid and tumor tissues and cells. Fc receptor staining occurs by the binding of Fc receptors present on cell to the Fc region of the primary and/or secondary antibody. Fc Receptor Block can be used for IHC (frozen and paraffin) flow cytometry, immunoflorescence and in-situ hybridization and for live cell functional assays.

IHC過程中，若是使用biotin及avidin的系統， 建議使用biotin/avidin的blocking buffer，在抗體反應之前，先移去內源性biotin/avidin。如此就不會有非一性binding的問題。

【產品特點與規格】

• 30 minute incubation step at room temperature
• Blocks Fc Receptors in both human and animal cells
• Eliminates false positive staining of white blood cells, lymphoid tissues, cytosmears (blood & bone marrow)
• Insures accurate lymphoma and leukemia typing
• Eliminates negative control staining of lymphomas, lymphoid tissues, and blood and bone marrow smears
• Eliminates false positive staining for Kappa and Lambda staining
• Eliminates false positive staining of Reed Sternberg cells
• A must for CD markers staining in immunohistochemistry, immunofluorescence
• A must for CD phenotyping via flow cytometry
• A must for Immunoglobulins (Igs) staining via immunohistochemistry, flow cytometry and immunofluorescence
• A must for kappa and lambda staining